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复合微生物菌剂在苏打盐碱土改良中的应用

吉林农业大学学报 2024年第2期46卷 290-296页

作者：庞宁张雪刘俊清胡琦琳张建峰吉林农业大学生命科学学院秸秆生物学与利用教育部重点实验室长春130118

为探究复合菌剂对盐碱土改良的影响,在分离筛选耐盐碱固氮菌寡养单胞菌属(Oligotrophomonas)和农杆菌属(Agrobacterium)基础上,与实验室现有的溶磷菌、纤维素降解菌进行组合,并通过水稻盆栽试验,验证了复合微生物菌剂在土壤理化性质、... 详细信息

为探究复合菌剂对盐碱土改良的影响,在分离筛选耐盐碱固氮菌寡养单胞菌属(Oligotrophomonas)和农杆菌属(Agrobacterium)基础上,与实验室现有的溶磷菌、纤维素降解菌进行组合,并通过水稻盆栽试验,验证了复合微生物菌剂在土壤理化性质、水稻形态及生理指标的作用效果。结果表明:不同处理均与对照(未处理盐碱土)差异显著(P<0.05),复合菌肥组土壤pH下降了0.92个单位,复合菌剂组电导率下降27.0%,生物炭基复合菌剂组HCO-3含量下降54.1%;生物炭基复合菌剂组有机质、速效氮磷钾等指标均明显增加;生物炭基复合菌剂组水稻的株高、根长、鲜质量、干质量分别比对照提高了391.5%,215.5%,1203.8%,1235.7%;生物炭基复合菌剂组水稻的SPAD、可溶性蛋白、SOD、根系活力等指标比对照分别增加了262.6%,138.3%,269.8%,298.7%,MDA下降了35.6%。总体来看,复合菌剂对苏打盐碱土有一定的改良效果,优于市面出售的菌剂,其与生物炭配施效果最佳,为苏打盐碱地的修复和改良提供技术支持。

关键词：复合菌剂水稻盐碱地生物炭

基于Hsp70基因的绵羊肺炎支原体TaqMan检测方法的建立及其遗传演化分析

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畜牧兽医学报 2024年第4期55卷 1684-1695页

作者：江锦秀张靖鹏林裕胜刘维巍胡奇林万春和福建省农业科学院畜牧兽医研究所福州350013 福建农林大学动物科学学院(蜂学学院) 福州350002

热休克蛋白70 (heat shock protein 70,Hsp70)是绵羊肺炎支原体(Mycoplasma ovipneumoniae, Movi)的重要膜蛋白,是机体内高度保守的生物分子,但在种间差异大,可作为分子生物学检测的候选靶区。为建立基于Hsp70基因的Movi通用型TaqMan实... 详细信息

热休克蛋白70 (heat shock protein 70,Hsp70)是绵羊肺炎支原体(Mycoplasma ovipneumoniae, Movi)的重要膜蛋白,是机体内高度保守的生物分子,但在种间差异大,可作为分子生物学检测的候选靶区。为建立基于Hsp70基因的Movi通用型TaqMan实时荧光定量PCR(qPCR)检测方法,并进一步了解其遗传变异情况,本研究基于GenBank中Movi的Hsp70基因特征,设计特异性的引物及探针,建立了基于Hsp70基因的绵羊肺炎支原体qPCR检测方法。应用建立的检测方法对88份山羊鼻拭子样品及43份疑似羊支原体性肺炎(Mycoplasmal pneumonia of sheep and goats, MPSG)病料进行检测。将检测结果为Movi阳性的肺组织样品进行分离鉴定,并对分离株Hsp70基因进行序列分析。结果显示,建立的qPCR检测方法其相关系数为1.00,扩增效率为96.0%,斜率为-3.411,Y轴截距为37.29。特异性强,与丝状支原体山羊亚种(Mycoplasma mycoides ***, Mmc)、山羊支原体山羊肺炎亚种(Mycoplasma capricolum ***, Mccp)、莱氏无胆甾原体(Acholeplasmalaidlawii, AL)、无乳支原体(Mycoplasma agalactiae, Maga)、伪结核棒状杆菌(Corynebacterium pseudotuberculosis, CP)、羊口疮病毒(orf virus, ORFV)、牛支原体(Mycoplasma bovis, Mb)等牛羊常见病原均无交叉反应;敏感性高,最低检测限为5.72 copies·μL^(-1);重复性优,组内变异系数和组间变异系数均小于1.00%。6株Movi分离株Hsp70基因全长均为1 818 bp,与其它Movi参考株核苷酸和氨基酸同源性分别为96.0%~99.4%和98.0%~100.0%;进一步分析发现,山羊源Movi均比绵羊源多1个N-糖基化位点。遗传演化分析表明,其均处于ClusterⅠA亚分支(均为山羊源)。综上,本研究建立了特异性强、敏感性高、重复性优的基于Hsp70基因的Movi的qPCR检测方法。序列分析发现,不同来源Movi的Hsp70基因核苷酸同源性高;遗传演化分析证实,Movi福建株与山羊源分离株遗传关系较近。本研究不仅为Movi临床诊断提供了技术支持,更为进一步了解Movi遗传演化规律提供参考。

关键词：绵羊肺炎支原体 Hsp70基因 TaqMan实时荧光定量PCR方法序列分析

Ultrafast Carbonized Wood of Electrode-Scaled Aligned-Porous Structure for High-Performance Lithium Batteries

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Transactions of Tianjin University 2023年第5期29卷 387-394页

作者： Shaojie Chen Lu Nie Hongsheng Shi Xiangchen hu Zeyu Wang Xinshui Zhang Yuyao Zhang qilin hu Tianyi Gao Yi Yu Wei Liu School of Physical Science and Technology Shanghai Tech UniversityShanghai 201210China Shanghai Key Laboratory of High-Resolution Electron Microscopy Shanghai Tech UniversityShanghai 201210China Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) Nankai UniversityTianjin 300071China

The use of carbonized wood in various functional devices is attracting considerable attention due to its low cost,vertical channels,and high electrical ***,the conventional carbonization method requires a long processing time and an inert ***,a microwave-assisted ultrafast carbonization technique was developed that carbonizes natural wood in seconds without the need for an inert atmosphere,and the obtained aligned-porous carbonized wood provided an excellent electrochemical performance as an anode material for lithium-ion *** ultrafast carbonization technique simultaneously produced ZnO nanoparticles during the carbonization process that were uniformly distributed on the alignedporous *** hierarchical structure of carbonized wood functionalized with ZnO nanoparticles was used as a host for achieving high-performance lithium-sulfur batteries:the highly conductive carbonized wood framework with vertical channels provided good electron transport pathways,and the homogeneously dispersed ZnO nanoparticles effectively adsorbed lithium polysulfide and catalyzed its conversion *** summary,a new method was developed to realize the ultrafast carbonization of biomass materials with decorated metal oxide nanoparticles.

关键词： Lithium–Sulfur battery Shuttle effect Lithium-ion battery Biomass carbonization

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汽车发动机悬置用天然橡胶胶料配方的研究

橡胶工业 2024年第10期71卷 754-758页

作者：肖勇明吕秀凤胡淇淋郭春杰李晓武博戈橡胶塑料(株洲)有限公司湖南株洲412007

对汽车发动机悬置用天然橡胶(NR)胶料配方进行研究。结果表明:采用半有效硫化体系(硫黄/促进剂用量比为1.7/1.5)、炭黑N330/炭黑N774/白炭黑(用量比11/18/5)补强体系、防老剂4020/防老剂RD/防老剂MB/微晶石蜡(用量比2/1/1/1)防护体系的N... 详细信息

对汽车发动机悬置用天然橡胶(NR)胶料配方进行研究。结果表明:采用半有效硫化体系(硫黄/促进剂用量比为1.7/1.5)、炭黑N330/炭黑N774/白炭黑(用量比11/18/5)补强体系、防老剂4020/防老剂RD/防老剂MB/微晶石蜡(用量比2/1/1/1)防护体系的NR胶料的动静刚度比较小,耐热空气老化性能较好;硫化温度过高和硫化时间过长易导致NR胶料出现硫化返原,使其动静刚度比增大,耐热空气老化性能下降。

关键词：汽车发动机悬置天然橡胶动静刚度比耐热空气老化性能

Detecting Double Mixed Compressed Images Based on Quaternion Convolutional Neural Network

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Chinese Journal of Electronics 2024年第3期33卷 657-671页

作者： Hao WANG Jinwei WANG Xuelong hu Bingtao hu qilin YIN Xiangyang LUO Bin MA Jinsheng SUN Department of Automation Nanjing University of Science and Technology Engineering Research Center of Digital Forensics Ministry of Education Department of Computer and Software Nanjing University of Information Science and Technology School of Management Nanjing University of Posts and Telecommunications Department of Computer Science and Engineering Guangdong Province Key Laboratory of Information Security Technology Sun Yat-sen University State Key Laboratory of Mathematical Engineering and Advanced Computing Qilu University of Technology

Detection of color images that have undergone double compression is a critical aspect of digital image *** the existence of various methods capable of detecting double Joint Photographic Experts Group(JPEG) compression,they are unable to address the issue of mixed double compression resulting from the use of different compression *** particular,the implementation of Joint Photographic Experts Group 2000(JPEG2000)as the secondary compression standard can result in a decline or complete loss of performance in existing *** tackle this challenge of JPEG+JPEG2000 compression,a detection method based on quaternion convolutional neural networks(QCNN) is *** QCNN processes the data as a quaternion,transforming the components of a traditional convolutional neural network(CNN) into a quaternion *** relationships between the color channels of the image are preserved,and the utilization of color information is ***,the method includes a feature conversion module that converts the extracted features into quaternion statistical features,thereby amplifying the evidence of double *** results indicate that the proposed QCNN-based method improves,on average,by 27% compared to existing methods in the detection of JPEG+JPEG2000 compression.

关键词： Image coding Image color analysis Quaternions Forensics Transform coding Color Feature extraction

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日本竞技体育发展策略研究

武汉体育学院学报 2017年第6期51卷 95-100页

作者：胡启林武汉理工大学体育部湖北武汉430070

21世纪日本实施了一系列竞技体育发展策略并取得显著成效,基本策略包括运动员训练与比赛补助机制、对举办国际赛事的地方团体资助机制、重视体育指导者的培训和国外进修、构筑运动员培养与选拔的一贯指导系统、发挥科技医学情报信息作... 详细信息

21世纪日本实施了一系列竞技体育发展策略并取得显著成效,基本策略包括运动员训练与比赛补助机制、对举办国际赛事的地方团体资助机制、重视体育指导者的培训和国外进修、构筑运动员培养与选拔的一贯指导系统、发挥科技医学情报信息作用。分析和总结21世纪日本竞技体育发展策略及其优势,对改革转型期的中国竞技体育制定适宜的发展策略有重要参考意义。

关键词：日本竞技体育发展策略体育事业体育政策

Detection and Identification of Avian, Duck, and Goose Reoviruses by RT-PCR: Goose and Duck Reoviruses Grouped the Same Specified Genogroup in Orthoreoviruses Genus SubgroupⅡ

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Detection and Identification of Avian, Duck, and Goose Reovi...

21世纪日本促进学生体质健康的举措及其启示

中国畜牧兽医学会家畜传染病学分会第六届全国会员代表大会暨第11次学术研讨会

作者： Yun Zhang Ming Liu Shuidong Ouyang qilin hu Zhou Han National Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Virology Divisions Fujian Husbandry and Veterinary Research Institute Veterinary Department Northeast Agriculture University

A reverse transcription-polymerase chain reaction (RT-PCR) procedure for the detection of avian, duck and goose reovirus (ARV, DRV and GRV) RNA from cell culture supernatant and clinical samples was established. The RNA of the virus was purified by the Trizol reagent and reverse transcription made by using random hexamers. The amplified, cloned, and sequenced 598-base pairs products on the σA-encoding gene fragment from 24 isolates (5 DRV, 3 GRV, and 8 ARV ranging more than 30 years) indicated that the primer regions were well conserved. The sensitivity of this method was determined to be 10 -2 PFU. Specificity of the RT-PCR method was determined by testing specimens containing avian influenza A viruses, newcastle disease virus, and Infectious bronchitis virus, all of which yielded negative results with no discernible background. The efficiency of the system for detection of ARV, DRV, and GRV directly in 71/83 clinical samples was confirmed. The nucleotide sequence analysis indicates that all the duck and goose reoviruses isolated from China different locale and year were closely related, showing 97.4-100 % identity to each other, but only with 86.7-88.5 % identity to French MDRV 89026. The homology of amplified σA-encoding gene and amino acids sequences in this study and GenBank were varied from 74.2-78.4% and 87.4-92.5%, respectively, between duck/geese and chicken species. Reoviruses σA-encoding gene segment shared 88.6-100% and 87.3-100% nucleotide identities, respectively, within duck/geese and chicken species. Phylogenetic analysis based on σA-encoding gene indicated that GRV with DRV segregated into the same specified genogroup within orthoreoviruses genus subgroup II and more related to ARV than to Nelson Bay virus (NBV). Overall, this study developed a sensitive and specific technique for the identification ARV, DRV and GRV and sequencing analysis enhanced our understudying of ARV, DRV, and GRV evolution relationship.

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武汉体育学院学报 2013年第10期47卷 23-27页

作者：陈莉胡启林武汉理工大学体育部湖北武汉430070

分析认为,21世纪日本实施了一系列促进学生体质健康的举措并收到了显著效果,其基本措施包括法律保障、把提高体力视为首要目标、强化体育课程改革、重视地域体育俱乐部向学生开放。而我国学生的身体素质指标多年来持续下滑。通过分析日... 详细信息

分析认为,21世纪日本实施了一系列促进学生体质健康的举措并收到了显著效果,其基本措施包括法律保障、把提高体力视为首要目标、强化体育课程改革、重视地域体育俱乐部向学生开放。而我国学生的身体素质指标多年来持续下滑。通过分析日本提高学生体能素质的举措及其优势,可以为制定符合我国国情的增强学生体质的策略提供参考。

关键词：日本增强学生体质举措启示

锂离子电池正极材料LiMn_(1.95)Mg_(0.05)O_(3.9)F_(0.1)的制备及其性能研究

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化工新型材料 2016年第12期44卷 170-173页

作者：刘奇琳胡启山武夷学院茶与食品学院武夷山354300 四川文理学院化学与工程技术学院达州635000

通过固相法制备出锂离子电池正极材料LiMn_2O_4和LiMn_(1.95)Mg_(0.05)O_(3.9)F_(0.1)样品,并通过XRD、SEM、EDS、充放电测试、CV和EIS对其结构、形貌以及电化学性能进行了研究。结果发现,适量Mg、F的掺杂未改变LiMn_2O_4的尖晶石结构。... 详细信息

通过固相法制备出锂离子电池正极材料LiMn_2O_4和LiMn_(1.95)Mg_(0.05)O_(3.9)F_(0.1)样品,并通过XRD、SEM、EDS、充放电测试、CV和EIS对其结构、形貌以及电化学性能进行了研究。结果发现,适量Mg、F的掺杂未改变LiMn_2O_4的尖晶石结构。在0.2C倍率下,样品LiMn_2O_4和LiMn_(1.95)Mg_(0.05)O_(3.9)F_(0.1)的首次放电比容量分别为121.3mAh/g和123.7mAh/g,循环60次后,容量保持率分别为82.1%和91.4%。在5C倍率下,样品LiMn_(1.95)Mg_(0.05)O_(3.9)F_(0.1)的放电比容量为92.4mAh/g,而LiMn_2O_4的放电比容量仅为76.5mAh/g。Mg、F的共同掺杂,可以有效抑制锰酸锂晶体中JahnTeller效应导致的结构畸变,稳定尖晶石结构,明显改善其循环稳定性和倍率性能,并提高材料的初始放电比容量。

关键词：锂离子电池正极材料 LiMn2O4 掺杂电化学性能