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机器学习鉴定KDELR3作为骨关节炎缺氧特征基因的实验验证

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中国组织工程研究 2024年第21期28卷 3431-3437页

作者：徐文飞明春玉梅其杰袁长深郭锦荣曾超段戡广西中医药大学第一附属医院四肢骨伤科广西壮族自治区南宁市530023 广西中医药大学附属瑞康医院老年病科广西壮族自治区南宁市530011

背景:缺氧与骨关节炎软骨细胞损伤的发生、发展密切相关,但具体作用靶点及调控机制尚不清楚。目的:运用机器学习方法鉴定KDEL(Lys-Asp-Glu-Leu)受体3(KDELR3)作为骨关节炎缺氧的特征基因及免疫浸润分析,以期为骨关节炎的治疗提供新的思... 详细信息

背景:缺氧与骨关节炎软骨细胞损伤的发生、发展密切相关,但具体作用靶点及调控机制尚不清楚。目的:运用机器学习方法鉴定KDEL(Lys-Asp-Glu-Leu)受体3(KDELR3)作为骨关节炎缺氧的特征基因及免疫浸润分析,以期为骨关节炎的治疗提供新的思路与方法。方法:从GEO数据库下载骨关节炎相关的数据集和GSEA网站中获取缺氧相关基因;采用R语言对骨关节炎数据集进行批次校正及免疫浸润分析,并提取骨关节炎缺氧基因进行差异分析,对差异表达基因进行GO功能及KEGG信号通路分析;同时运用加权基因共表达网络分析(Weighted correlation network analysis,WGCNA)及机器学习筛选骨关节炎缺氧的特征基因,并进行体外细胞实验,运用数据集及qPCR验证表达并行相关免疫浸润分析。结果与结论:①经批次校正及主成分分析获得骨关节炎基因8492个,主要与Macrophages M2和Mast cells resting等免疫细胞密切相关;同时获得缺氧基因200个,进而得到41个骨关节炎缺氧差异表达基因。②GO分析主要涉及对营养水平、糖皮质激素反应等生物过程;涉及溶酶体腔、高尔基内腔等细胞组分;涉及14-3-3蛋白结合、DNA结合转录激活剂活性等分子功能。③KEGG分析骨关节炎缺氧差异表达基因与PI3K-Akt、FoxO及癌症中的微小RNA等信号通路有关。④运用WGCNA分析及机器学习筛选后获得特征基因KDELR3。⑤通过基因芯片验证后发现KDELR3基因在滑膜中实验组基因表达高于对照组(P=0.014),而半月板中实验组基因的表达却低于对照组(P=0.024)。⑥体外软骨细胞实验显示KDELR3基因在软骨中实验组表达高于对照组(P=0.005),同时KDELR3基因与Macrophages M0(P=0.014),T cells follicular helper(P=0.014)等密切相关。运用机器学习方法证实KDELR3可作为骨关节炎缺氧特征基因,可能通过改善缺氧来干预骨关节炎发病,期待能为更好地治疗骨关节炎提供新方向。

关键词：骨关节炎机器学习缺氧特征基因软骨细胞生物标志物免疫浸润分析

Monitoring landslide associated with reservoir impoundment using synthetic aperture radar interferometry: A case study of the Yalong reservoir

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Geodesy and Geodynamics 2022年第2期13卷 138-150页

作者： Zhe Liu Bing xu qijie Wang Wenyan Yu Zelang Miao School of Geosciences and Info-Physics Central South UniversityChangsha 410083China

The construction of large reservoirs can address the problem of uneven distribution of rivers in time and space,thereby meeting the needs of human production and living.However,the huge elevation of the water level in some areas may modify the distribution of the groundwater level,causing geological disasters,such as surface deformation and landslides.The Yalong reservoir supplies water to the downstream area of Shannan,Tibet;however,since the reservoir started storing water in 2017,the government has discovered two ancient landslides.In this study,to monitor the deformation of the Yalong reservoir since its construction in 2014,we first used synthetic aperture radar(SAR) data and the multidimensional small baseline subset(MSBAS) method to obtain the deformation in the east-west and vertical directions.The result indicated the presence of three large,slow-moving landslides:Landslides I and II,located on the right bank of the Yalong reservoir,which are consistent with the results obtained by the actual survey,and a new discovery,Landslide Ⅲ,located on the left side of the reservoir.Meanwhile,the experimental results indicated that the dam had undergone obvious deformation after impoundment,which should not be ignored.The global positioning system and interferometric SAR(InSAR) timeseries deformation residual data were used to verify the accuracy of the InSAR method.The results also showed that the deformation caused by the three landslides had te nded to accele rate after the rese rvoir’s impoundment,and that the failure mode was retrogressive landslide.We found that InSAR plays a vital role in landslide detection and failure mode research around reservoirs,and assists in providing early warning for subsequent landslide disasters.

关键词： Landslide monitoring Two-dimensional deformation Yalong reservoir SBAS-InSAR

Resource utilization of high concentration SO_(2) for sulfur production over La-Ce-O_(x)@ZrO_(2) composite oxide catalyst

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Journal of Rare Earths 2023年第12期41卷 1945-1952,I0005页

作者： Yao Lu qijie Jin Wenyu Ji Chengzhang Zhu Mutao xu Yingying Zhu Haitao xu School of Environmental Science and Engineering Nanjing Tech UniversityNanjing210009China State Key Laboratory of Materials-Oriented ChemicalEngineering Nanjing Tech University Nanjing20009China College of Materials Science and Engineering Nanjing Tech UniversityNanjing210009China

Sulfur dioxide is one of the main causes of air pollution such as acid rain and photochemical smog,and its pollution control and resource utilization become an important research direction of air pollution control,The active component La-Ce-O_(x) is loaded on SiO_(2),γ-Al_(2)O_(3),TiO_(2) and ZrO_(2),and the La-Ce-Ox@ZrO_(2)exhibits the best catalytic activity.By adjusting the loading amount of La-Ce-O_(x),La-Ce-Ox@ZrO_(2) with different mass fractions was prepared.The results show that the activity of 15%La-Ce-Ox@ZrO_(2)catalyst is the best.The SO_(2)conversion is 100%,and the S yield and S selectivity are more than 96% at 350℃.According to the analysis results of H_(2)-TPR,CO_(2)-TPD and NH_(3)-TPD,ZrO_(2) as a support not only reduces the acidity of the catalyst,but also improves the weak alkaline sites of the catalyst,which is conducive to the adsorption and activation of SO2molecules at low temperature.The incorporation of La and Ce increases the oxygen concentration adsorbed on the catalyst.The strong interaction between the support ZrO_(2) and the active component La-Ce-Oxis conducive to the electron transfer between the active component and the support,and improves the activity of the catalyst.For the 15%La-Ce-O_(x)@ZrO_(2),the main reaction intermediates are weakly adsorbed SO_(2)(SO_(3)^(2-)),bicoordinated CO_(3)^(2-),monodentate carbonate and CO in the gas phase.Therefore,the catalytic reaction follows both L-H and E-R mechanisms.

关键词： La-Ce-Ox@ZrO_(2) Sulfur dioxide Carbon monoxide Resource utilization Catalytic mechanism Rare earths

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深圳社区建设标准指导体系研究

城市建筑 2024年第6期21卷 73-78页

作者：徐祺杰张展鹏董恬深圳市城市规划设计研究院股份有限公司

社区是人民日常生活的基础空间,文章通过梳理国内外社区相关理论概念,发现其中包含基本的共性内容,同时各概念对社区的关注点亦各有侧重。结合对未来深圳社区建设发展趋势的研判,文章提出了更全面、可供选择的社区建设标准指导体系,包括... 详细信息

社区是人民日常生活的基础空间,文章通过梳理国内外社区相关理论概念,发现其中包含基本的共性内容,同时各概念对社区的关注点亦各有侧重。结合对未来深圳社区建设发展趋势的研判,文章提出了更全面、可供选择的社区建设标准指导体系,包括5大类16项,涵盖81分项建设内容。同时按照配置要求分级,把要素分为底线控制的基础保障型、可供选择的品质提升型和创新集成的特色引导型三种,覆盖不同社区需求,以打造更加美好宜居的未来社区。

关键词：深圳社区完整社区未来社区生活圈建设标准

WGCNA和机器学习识别骨关节炎铁死亡特征基因及实验验证

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中国组织工程研究 2024年第30期28卷 4909-4914页

作者：徐文飞明春玉段戡袁长深郭锦荣胡琪曾超梅其杰广西中医药大学第一附属医院四肢骨伤科广西壮族自治区南宁市530023 广西中医药大学附属瑞康医院老年病科广西壮族自治区南宁市530011

背景:铁死亡与骨关节炎发生、发展密切相关,但具体特征基因及调控机制尚不清楚。目的:运用WGCNA及多种机器学习方法识别骨关节炎铁死亡特征基因及免疫浸润分析。方法:从GEO数据库下载骨关节炎相关数据集,同时在FerrDb网站中获取铁死亡... 详细信息

背景:铁死亡与骨关节炎发生、发展密切相关,但具体特征基因及调控机制尚不清楚。目的:运用WGCNA及多种机器学习方法识别骨关节炎铁死亡特征基因及免疫浸润分析。方法:从GEO数据库下载骨关节炎相关数据集,同时在FerrDb网站中获取铁死亡相关基因,采用R语言对骨关节炎数据集进行批次校正、提取骨关节炎铁死亡基因并进行差异分析,对差异基因进行GO功能及KEGG信号通路分析;同时运用WGCNA分析及机器学习(随机森林、LASSO回归及SVM-RFE分析)筛选骨关节炎铁死亡特征基因,并进行体外细胞实验,将软骨细胞分为正常组和骨关节炎组,运用数据集及qPCR验证表达并行相关免疫浸润分析。结果与结论:①经批次校正及PCA分析获得骨关节炎基因12548个,同时获得铁死亡基因484个,进而得到24个骨关节炎铁死亡差异基因;②GO分析主要涉及对氧化应激反应、对有机磷反应等生物过程;涉及细胞顶端、顶端质膜等细胞组分;涉及血红素结合、四吡咯结合等分子功能;③KEGG分析显示,骨关节炎铁死亡差异基因与白细胞介素17信号通路、肿瘤坏因子信号通路等信号通路有关;④运用WGCNA分析及机器学习筛选后获得特征基因KLF2;通过基因芯片验证后发现实验组半月板组织中KLF2基因表达高于对照组(P=0.00014);⑤体外细胞实验显示,骨关节炎组软骨细胞中Ⅱ型胶原、KLF2基因表达低于对照组(P<0.05),同时在骨关节炎铁死亡中肥大细胞与树突状细胞密切相关(r=0.99),KLF2与自然杀伤细胞(r=-1,P=0.017)、滤泡辅助性T细胞(r=-1,P=0.017)等密切相关;⑥结果显示,运用WGCNA分析及机器学习方法证实KLF2可作为骨关节炎铁死亡的特征基因,可能通过干预KLF2来改善骨关节炎铁死亡。

关键词： WGCNA分析机器学习骨关节炎铁死亡特征基因免疫浸润分析体外细胞实验

Enhanced secondary organic aerosol formation during dust episodes by photochemical reactions in the winter in Wuhan

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Journal of Environmental Sciences 2023年第11期133卷 70-82页

作者： Kai xu Yafei Liu Chenlu Li Chen Zhang Xingang Liu qijie Li Min Xiong Yujun Zhang Shijie Yin Yu Ding State Key Laboratory of Water Environment Simulation School of EnvironmentBeijing Normal UniversityBeijing 100875China Wuhan Municipality Environmental Monitoring Center Wuhan 430015China College of Environment and Ecology Chongqing UniversityChongqing 400030China

To investigate the effect of frequently occurring mineral dust on the formation of secondary organic aerosol(SOA),106 volatile organic compounds(VOCs),trace gas pollutants and chemical components of PM_(2.5) were measured continuously in January 2021 in Wuhan,Central China.The observation period was divided into two stages that included a haze period and a following dust period,based on the ratio of PM_(2.5) and PM_(10) concentrations.The average ratio of secondary organic carbon(SOC)to elemental carbon(EC)was 1.98 during the dust period,which was higher than that during the haze period(0.69).The contribution of SOA to PM_(2.5) also increased from 2.75% to 8.64%.The analysis of the relationships between the SOA and relative humidity(RH)and the odd oxygen(e.g.,O_(X)=O_(3)+NO_(2))levels suggested that photochemical reactions played a more important role in the enhancement of SOA production during the dust period than the aqueous-phase reactions.The heterogeneous photochemical production of OH radicals in the presence of metal oxides during the dust period was believed to be enhanced.Meanwhile,the ratios of trans-2-butene to cis-2-butene and m-/p-xylene to ethylbenzene(X/E)dropped significantly,confirming that stronger photochemical reactions occurred and SOA precursors formed efficiently.These results verified the laboratory findings that metal oxides in mineral dust could catalyse the oxidation of VOCs and induce higher SOA production.

关键词： VOCs Mineral dust Metal oxides SOA Photochemical reactions

金属负载量对CuO/NiO-CeO_(2)催化CO-Prox性能的影响

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石油化工高等学校学报 2024年第2期37卷 42-49页

作者：徐琪杰王宏浩吕丽荣姜雅新侯晓宁张磊高志贤辽宁石油化工大学石油化工学院辽宁抚顺113001 北京市科学技术研究院资源环境研究所北京100095 北京中医药大学东方学院医学检验技术学院河北沧州061108 山西师范大学化学与材料科学学院山西太原030000

为深度去除富氢气中的CO,制备CO-Prox催化性能较好的催化剂是目前的研究热点。采用分步浸渍法制备了CuO/NiO-CeO_(2)催化剂,通过XRD、BET、H_(2)-TPR、HR-TEM等手段对催化剂进行表征,探究了金属Cu+Ni的负载量(金属负载量)对催化剂结构... 详细信息

为深度去除富氢气中的CO,制备CO-Prox催化性能较好的催化剂是目前的研究热点。采用分步浸渍法制备了CuO/NiO-CeO_(2)催化剂,通过XRD、BET、H_(2)-TPR、HR-TEM等手段对催化剂进行表征,探究了金属Cu+Ni的负载量(金属负载量)对催化剂结构、还原性能及其CO-Prox性能的影响。结果表明,CuO/NiO-CeO_(2)催化剂中均形成了Cu/Ni-O-Ce固溶体;催化活性主要与高度分散在载体表面的Cu物种和固溶体的浓度有关;当金属负载量为8%时,高度分散在载体表面的Cu物种和固溶体的浓度较高,催化剂表现出较好的催化活性;在CO/H_(2)/CO_(2)/O_(2)/Ar气氛下、反应温度为130℃、氧过量系数为1.2、质量空速为20266 mL/(g·h)的条件下,CO转化率为95.9%,CO氧化选择性为86.3%。

关键词：金属负载量 CuO/NiO-CeO_(2) CO-Prox催化性能高分散Cu物种固溶体

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Ag-Catalyzed decarboxylative acylation of pyridazines using a-keto acids in aqueous media

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Ag-Catalyzed decarboxylative acylation of pyridazines using ...

Study on undercooling of metal droplet in rapid solidification

河南省化学会2020年学术年会

作者： Shengqiang Wang Xiaobo xu qijie xu The College of Chemistry and Pharmaceutical Engineering Huanghuai University

Pyridazine scaffold is an important class of nitrogen-containing heterocycles which can be frequently found in agrochemistry, functional materials and supramolecular chemistry,meanwhile,the pyridazines motifs are present in an increasing numbers of pharmaceutical products. Acylated pyridazines represent versatile intermediates,the carboxyl can be converted to other different functional groups.In this paper,an efficient and general protocol for Ag-Catalyzed decarboxylative acylation of pyridazines usingα-keto acids as acylation reagent in aqueous media has been described.This method provides a new avenue for the synthesis of diverse array of acylated pyridazines with different substitution patterns.The reaction proceeds smoothly in water under mild conditions and exhibits a good functional group tolerance.

关键词： Pyridazines Ag-Catalyzed α-Keto acids Decarboxylative acylation

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Science China(Technological Sciences) 2005年第6期48卷 632-637页

作者： GAO Yulai GUAN Wanbing ZHAI qijie xu Kuangdi School of Materials Science and Engineering Shanghai University Shanghai 200072 China School of Materials Science and Engineering Shanghai University Shanghai 200072 China School of Materials Science and Engineering Shanghai University Shanghai 200072 China School of Materials Science and Engineering Shanghai University Shanghai 200072 China

A mathematical model for the undercooling of the metal droplet during the rapid solidification is established, by which the factors that influence the undercooling of the metal droplet during the rapid solidification are analyzed, and the parameter ζ=σSL3/ (TL?H 2 ) is defined as the impact factor of the undercooling for the droplet solidification. Different undercoolings of droplets induced by various rapid solidification conditions are mainly ascribed to the change of the impact factor. Moreover, it is shown that the larger of ζ, the higher the relative undercooling can be gained. Meanwhile, the parameters such as solid-liquid interfacial energy σSL and latent heat of solidification ?H also vary with the rapid solidification conditions of droplets.

关键词： droplet, rapid solidification, relative undercooling, impact factor.