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OsMKKK70 regulates grain size and leaf angle in rice through the OsMKK4-OsMAPK6-OsWRKY53 signaling pathway

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Journal of Integrative Plant Biology 2021年第12期63卷 2043-2057页

作者： Zhiqi Liu Enyang Mei Xiaojie Tian Mingliang He Jiaqi Tang Min xu Jiali Liu Lu Song Xiufeng Li Zhenyu Wang Qingjie Guan qijiang xu Qingyun Bu Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040China College of Life Science Northeast Forestry UniversityHarbin 150040China Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design BreedingCASHarbin 150081China University of Chinese Academy of Sciences Beijing 100049China School of Laboratory Medicine Youjiang Medical University for NationalitiesBaise 533000China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Grain size and leaf angle are key agronomic traits that determine final yields in ***,the underlying molecular mechanisms are not well *** we demonstrate that the Oryza sativa Mitogen Activated Protein Kinase Kinase Kinase OsMKKK70 regulates grain size and leaf angle in *** OsMKKK70 caused plants to produce longer *** osmkkk62/70 double mutant and the osmkkk55/62/70 triple mutant displayed significantly smaller seeds and a more erect leaf angle compared to the wild type,indicating that OsMKKK70 functions redundantly with its homologs Os MKKK62 and Os *** analysis demonstrated that OsMKKK70 is an active kinase and that OsMKKK70 interacts with Os MKK4 and promotes Os MAPK6 *** addition,the osmkkk62/70 double mutant showed reduced sensitivity to Brassinosteroids(BRs).Finally,overexpressing constitutively active Os MKK4,Os MAPK6,and Os WRKY53 can partially complement the smaller seed size,erect leaf,and BR hyposensitivity of the osmkkk62/70 double *** together,these findings suggest that OsMKKK70 might regulate grain size and leaf angle in rice by activating Os MAPK6 and that OsMKKK70,Os MKK4,Os MAPK6,and Os WRKY53 function in a common signaling pathway that controls grain shape and leaf angle.

关键词： Brassinosteroids MAPK signaling OsMKKK70 rice seed size

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中国与哈萨克斯坦小麦生产比较分析

现代农业科技 2024年第9期 183-185,193页

作者：陈传信张永强聂石辉赛力汗·赛徐其江雷钧杰新疆农业科学院粮食作物研究所新疆乌鲁木齐830091 农业农村部荒漠绿洲作物生理生态与耕作重点实验室新疆乌鲁木齐830091

基于联合国粮食及农业组织提供的统计数据,比较中国和哈萨克斯坦两国小麦生产的差异。结果表明,中国小麦种植面积从1992年开始波动减少,2004年达到最低,2004年以后种植面积小幅增加并呈现平稳态势;哈萨克斯坦小麦种植面积出现波动,整体... 详细信息

基于联合国粮食及农业组织提供的统计数据,比较中国和哈萨克斯坦两国小麦生产的差异。结果表明,中国小麦种植面积从1992年开始波动减少,2004年达到最低,2004年以后种植面积小幅增加并呈现平稳态势;哈萨克斯坦小麦种植面积出现波动,整体趋于稳定。中国小麦单产从1992年开始在波动中逐渐提高,单产增加明显;哈萨克斯坦小麦单产虽有波动但整体比较平稳,未有明显提升。中国小麦总产从1992年开始在波动变化中基本表现为先增加后降低再增加的趋势,2021年总产达到最高,较1992年增加了34.80%;哈萨克斯坦小麦总产整体呈现减少并趋于稳定的态势。中国小麦种植面积、单产、总产均明显高于哈萨克斯坦。中国小麦总产的提高主要来源于单产的增加。中国小麦生产相关技术实践可为哈萨克斯坦小麦生产提供参考。

关键词：中国哈萨克斯坦小麦种植面积产量比较分析

Clinical application of intraperitoneal chemotherapy before surgery for ovarian cancer and its evaluation

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The Chinese-German Journal of Clinical Oncology 2009年第5期8卷 260-264页

作者： Dianyun Shi Mei Xiao Saiying Chen Bin Yang qijiang Zhu Pingping xu Chenfang Su Department of Obstetrics and Gynecology 81 Hospital of PLA Nanjing 210002 China

Objective： To investigate the clinical efficacy of intraperitoneal chemotherapy before surgery for ovarian cancer. Methods： 60 patients with stages Ⅱ-Ⅳ of ovarian cancer were treated with intraperitoneal chemotherapy of CAP or TP regimen followed by a surgery treatment and another chemotherapy for 6 cycles. And then the efficiency of the therapy was evaluated by analyzing the changes of ascites, the serum CA-125 and CA-19-9 levels and the findings in the operation, and investigating the recurrence of cancer and the survival. Results： After 1-3 cycles of intraperitoneal chemotherapy, serum levels of CA-125 and CA-19-9 and carcinous ascites significantly reduced in all patients, ascites reduce was over 50% in 98.3% cases; all cases were successfully treated with cytoreduction and it was found during the operation that bulky tumor was reduced and looser so as to be easily isolated and removed; PFS of the patients was prolonged, while the toxicity and side-effects were not so serious as beyond the patient toleration. Conclusion： Intraperitoneal chemotherapy before surgery for ovarian cancer has an active efficacy in clinic, being able to improve conditions of surgery and increase the opportunity of maximal cytoreduction, and prolong survival of the patients, and should be a good selection for the treatment of advanced ovarian cancer.

关键词： ovarian cancer intraperitoneal chemotherapy clinical application evaluation

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临床生物化学检验课程的教学改革研究与实践

科技风 2024年第11期 133-135页

作者：罗艳红陆群凤唐玉莲徐启江董明右车志群右江民族医学院医学检验学院广西百色533000

临床生物化学检验作为医学领域重要的辅助手段,对于诊断、治疗和疾病预防发挥着关键作用。然而,在教学过程中,我们面临着一些挑战,如如何做好质量控制、提高学生的职业认同感、提倡科学精神、提高民族自豪感以及培养医者仁心等。因此,... 详细信息

临床生物化学检验作为医学领域重要的辅助手段,对于诊断、治疗和疾病预防发挥着关键作用。然而,在教学过程中,我们面临着一些挑战,如如何做好质量控制、提高学生的职业认同感、提倡科学精神、提高民族自豪感以及培养医者仁心等。因此,本文旨在探讨临床生物化学检验课程的教学改革,并提出相应的措施,以提高教学质量和学生的综合素养,为学生将来服务好社会打下坚实的基础,并为相关兄弟院校提供参考。

关键词：临床生物化学检验教学改革研究与实践

Detection of GLV, OYDV and LYSV in potato onion (Allium cepa L., Aggregatum group) by RT-PCR

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Journal of Northeast Agricultural University(English Edition) 2007年第1期14卷 9-13页

作者： xu qijiang CHEN Dian TONG Youli LI Yuhua Research Institute of Flower Biotechnology Northeast Forestry University Harbin 150040 China College of Horticulture Northeast Agricultural University Harbin 150030 China

Three pairs of primers were designed and synthesized from nucleotide sequences of garlic latent virus （GLV）, onion yellow dwarf virus （OYDV）, and leek yellow stripe virus （LYSV） by using PCR primer design software. The expected fragments about 170 bp, 287 bp, and 191 bp were amplified by RT-PCR for GLV, OYDV, and LYSV, respectively in disease-infected plants of potato onion （Allium cepa L., Aggregatum group）, but such fragments were not obtained from healthy-looking plants and virus-free seedlings of shoot-tips. The amplified products ofGLV, OYDV and LYSV were cloned into pGEM-T vectors, and transformed into Escherichia coli. JM109. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank by performing a NCBI BLAST. The analysis showed that their homology attained 75% to 90%,89.5% to 96.1%,and 91.6% to 96.3% in GLV, OYDV, and LYSV, respectively. The total RNA of 6.34 ug·uL^-1 from infected plants was diluted to a series of 10^-1 to 10^5 and the detection sensitivity of RT-PCR was 10^4 （about 4 ng）. Thus, a method of identification and detection by RT-PCR of GLV, OYDV, and SLYV was established.

关键词： garlic latent virus (GLV) onion yellow virus (OYDV) leek yellow stripe virus (LYSV) RT-PCR

The Formation and Evolution of the Lujiang-Zongyang Volcano-Structural Depression in Anhui and Its Relation to Mineralization

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Acta Geologica Sinica(English Edition) 1993年第4期67卷 411-427页

作者： Ren qijiang, Wang Dezi, xu Zhaowen, Dong Huogen, Pan Longquan, Yang Rongyong, Fang Changquan and Hu Jin’an Department of Earth Sciences, Nanjing University, Nanjing, Jiangsu Fei Zhenbi Department of Earth Sciences Nanjing University Nanjing Jiangsu

The Lujiang-Zongyang volcano-structural depression resulted from the combined action of tectonism and volcanism. Its evolution progressed through five stages from 190 to 105 Ma B.P., during which nine types of metal and pyrite deposits were formed. These deposits show obvious regularities in the temporal-spatial distribution and have certain genetic relation, forming a minerogenetic series related to shoshonite within or around the volcano-structural depression on the basement that sank for a long period of time. This paper suggests a four-dimensional model of the formation and evolution of a typical volcano-structural depression and its related mineralizalion in a transition belt from the active margins to the inner part of a plate under alternating shear-compressive and shear-extensional stress conditions, but predominantly shear-extensional stress conditions.

关键词： Lujiang-Zongyang Anhui Province volcano-structural depression stress field mineralization four-dimensional model

基于人工鱼群优化的直推式支持向量机分类算法

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计算机应用与软件 2013年第3期30卷 294-296页

作者：齐芳冯昕徐其江天津体育学院体育文化传媒系计算机教研室天津300381 天津音乐学院网管中心天津300171 山东信息职业技术学院山东潍坊261061

提出基于人工鱼群优化的直推式支持向量机分类算法。该算法使直推式学习思想的优势得到充分的展现,在部分UCI标准数据集和20-Newgroups文本实验数据集上的对比实验表明,该算法较经典支持向量机算法和基于蚁群算法的直推式支持向量机算... 详细信息

提出基于人工鱼群优化的直推式支持向量机分类算法。该算法使直推式学习思想的优势得到充分的展现,在部分UCI标准数据集和20-Newgroups文本实验数据集上的对比实验表明,该算法较经典支持向量机算法和基于蚁群算法的直推式支持向量机算法具有更高的分类性能。

关键词：直推式学习支持向量机人工鱼群算法

大蒜MADS-box基因AsPI的克隆及表达分析

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园艺学报 2017年第1期44卷 69-79页

作者：徐熔任洪杰王海竹娄虎徐启江东北林业大学生命科学学院哈尔滨150040 杭州和壹基因科技有限公司杭州310052

B类MADS-box基因在调控显花植物的花瓣和雄蕊发育过程中发挥关键作用。为初步了解大蒜花发育的分子机制,以‘阿城紫皮’大蒜(Allium sativum)的花蕾为试材克隆了AsPI(Gen Bank登录号为KY272748)。AsPI的cDNA长939 bp,开放阅读框为615 bp... 详细信息

B类MADS-box基因在调控显花植物的花瓣和雄蕊发育过程中发挥关键作用。为初步了解大蒜花发育的分子机制,以‘阿城紫皮’大蒜(Allium sativum)的花蕾为试材克隆了AsPI(Gen Bank登录号为KY272748)。AsPI的cDNA长939 bp,开放阅读框为615 bp,编码204个氨基酸。推导的氨基酸序列显示,AsPI编码的蛋白质包含高度保守的MADS结构域(1~57氨基酸)和保守的K结构(76~140氨基酸),AsPI蛋白具有PI亚家族特有的序列特征:MADS区特征丝氨酸残基、K区高度保守序列KHExL以及C–末端的PI基序。序列比对、功能域分析及系统发育分析结果表明,AsPI属于单子叶植物B功能基因PI亚家族。半定量和实时RT-PCR检测AsPI在大蒜不同组织器官中的表达模式,结果表明,在花蕾中高丰度表达,在花茎中表达量中等,在根、假茎、嫩叶、保护叶等营养器官中表达量极低甚至不表达。

关键词：大蒜花器官发育 B功能MADS-box基因 AsPI基因

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分蘖洋葱与大蒜间作对大蒜开花的化感作用

园艺学报 2019年第3期46卷 464-472页

作者：徐墨航张冰伟娄虎刘学徐启江哈尔滨工业大学附属中学哈尔滨150086 东北林业大学生命科学学院哈尔滨150040

以大蒜(Allium sativum)‘伊宁红皮’和抽薹型分蘖洋葱(Allium cepa var. aggregatum)‘M-4’为试验材料,通过间作,分析分蘖洋葱对大蒜开花的影响。结果表明,分蘖洋葱与大蒜间作,可以显著提高大蒜植株生长势,平均株高和假茎粗分别为96.3... 详细信息

以大蒜(Allium sativum)‘伊宁红皮’和抽薹型分蘖洋葱(Allium cepa var. aggregatum)‘M-4’为试验材料,通过间作,分析分蘖洋葱对大蒜开花的影响。结果表明,分蘖洋葱与大蒜间作,可以显著提高大蒜植株生长势,平均株高和假茎粗分别为96.3 cm和10.6 mm,比对照分别增高6.1 cm,增粗2.4 mm。间作的分蘖洋葱可促进大蒜的生殖发育,抽薹期比对照提前4 d,功能性大蒜小花数增多,减少气生鳞茎的数和大小,而且上调花分生组织属性基因gaLFY和AsFUL的转录水平。抽薹型分蘖洋葱与大蒜间作具有促进大蒜功能花发育的化感效应,有利于实现大蒜育性恢复。

关键词：大蒜分蘖洋葱化感作用开花