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Genome assembly of wild loquat (Eriobotrya japonica) and resequencing provide new insights into the genomic evolution and fruit domestication in loquat

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Horticulture Research 2023年第2期10卷 147-159页

作者： Danlong Jing Xinya Liu Qiao He Jiangbo Dang Ruoqian Hu Yan Xia Di Wu Shuming Wang Yin Zhang Qingqing Xia Chi Zhang Yuanhui Yu qigao guo guolu Liang Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China Academy of Agricultural Sciences of Southwest University State Cultivation Base of Crop Stress Biology for Southern Mountainous LandChongqing 400715China

Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved ***,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding *** of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its *** resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic *** with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic ***,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication ***-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development *** those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit *** high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat.

关键词： process insight breeding

Effects of Different Fertilization Treatments on Soil, Leaf Nutrient and Fruit Quality of Citrus grandis var. longanyou

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World Journal of Engineering and Technology 2017年第2期5卷 1-14页

作者： Rongfei Li Yaodong Chang Tao Hu Xueyou Jiang guolu Liang Zhiming Lu Youwen Yi qigao guo College of Horticulture and Landscape Architecture Southwest University Beibei Chongqing 400716 China Guang’an District Agricultural Bureau Guang'an Sichuan 638500 China Guang’an Science and Technology Development and Training Center Guang'an Sichuan 638500 China

The five-year-old “Longanyou” trees were used as the experimental material to study the effects of different fertilization treatments. The nutrient contents in soil and leaves, fruit yield and quality were determined, and then the correlations were analyzed. The results showed that: 1) The soil nutrient contents of 0 - 20 cm depth were more than the 20 - 40 cm, and the trends of nutrient contents of the 0 - 20 cm soil layers were as follows: treatment 2 (T2) > treatment 3 (T3) > treatment 4 (T4) > treatment 1(T1) > control (CK). However, the 20 - 40 cm depth had not significant difference between different treatments, but T2, T4 and T3 were higher than T1 and CK. It indicated that the soil effective nutrient content increased in T2 and T3. 2) Compared with the control, the content of K and B elements was improved obviously in leaves with the increase of organic manure application. The contents of P (1.60 g·kg-1), B (26.00 mg·kg-1) and Mg (1.18 g·kg-1) were the highest, and other nutrients contents were also higher, indicating that T2 could effectively improve the leaves’ nutrient contents. 3) The fruit yield per plant was the highest in T2 (95.40 kg plant-1), and the single fruit weight, total sugar, sugar and acid ratio, vitamin C were also the highest, but titratable acid was lower. It indicated that T2 effectively improved fruit yield and quality. 4) There were positive correlations between multiple factors of soil nutrients and the quality index, such as fruit peel thickness, total sugar, solid acid ratio, sugar and acid ratio, Vc content and single yield etc. There was significant correlation between K, B, Zn, Fe contents and fruit yield and quality index, and the contents of B, Zn and Fe in leaves were significantly correlated with soil nutrient, indicating that the contents of K, B, Zn, Fe in soil and leaf were closely related to fruit yield and quality. In sum, the T2 was the best fertilization scheme for orchard management practice of “Longanyou”.

关键词： Citrus grandis var. longanyou Fertilization Soil Nutrient Leaf Nutrient Fruit Quality

High-quality genome assemblies for two Australimusa bananas(Musa spp.)and insights into regulatory mechanisms of superior fiber properties

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Plant Communications 2024年第1期5卷 245-261页

作者： Run Zhou Shuo Wang Ni Zhan Weidi He Guiming Deng Tongxin Dou Xi-Tong Zhu Wen-Zhao Xie Yu-Yu Zheng Chunhua Hu Fangcheng Bi Huijun Gao Tao Dong Siwen Liu Chunyu Li Qiaosong Yang Lingqiang Wang Jia-Ming Song Jiangbo Dang qigao guo Ganjun Yi Ling-Ling Chen Ou Sheng Institute of Fruit Tree Research Guangdong Academy of Agricultural SciencesKey Laboratory of South Subtropical Fruit Biology and Genetic Resource UtilizationMinistry of Agriculture and Rural AffairsGuangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree ResearchGuangzhou 510640China College of Informatics Huazhong Agricultural UniversityWuhan 430070China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004China College of Horticulture and Landscape Architecture Southwest UniversityChongqing 400715China

Bananas(Musa spp.)are monocotyledonous plants with high genetic diversity in the Musaceae family that are cultivated mainly in tropical and subtropical *** fruits are a popular food,and the plants themselves have diverse *** genetic groups(genomes)are thought to have contributed to current banana cultivars:Musa acuminata(A genome),Musa balbisiana(B genome),Musa schizocarpa(S genome),and species of the Australimusa section(T genome).However,the T genome has not been effectively ***,we present the high-quality TT genomes of two representative accessions,Abaca(Musa textilis),with high-quality naturalfiber,and Utafun(Musa troglodytarum,Fe’i group),with abundant *** the Abaca and Utafun assemblies comprise 10 pseudochromosomes,and their total genome sizes are 613 Mb and 619 Mb,*** genome analysis revealed that the larger size of the T genome is likely attributable to rapid expansion and slow removal of *** with those of Musa AA or BB accessions or sisal(Agava sisalana),Abacafibers exhibit superior mechanical properties,mainly because of their thicker cell walls with a higher content of cellulose,lignin,and *** of MusaCesA cellulose synthesis genes peaks earlier in Abaca than in AA or BB accessions during plant development,potentially leading to earlier cellulose accumulation during secondary cell wall *** Abaca-specific expressed gene MusaMYB26,which is directly regulated by MusaMYB61,may be an important regulator that promotes precocious expression of secondary cell wall ***,MusaWRKY2 and MusaNAC68,which appear to be involved in regulating expression of MusaLAC and MusaCAD,may at least partially explain the high accumulation of lignin in *** work contributes to a better understanding of banana domestica-tion and the diverse genetic resources in the Musaceae family,thus providing resources for Musa genetic improvement.

关键词： Australimusa bananas T genome Musaceae evolution structural variations fiber

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植物多倍体诱导育种研究进展

生物学通报 2000年第2期35卷 8-10页

作者：郭启高宋明梁国鲁西南农业大学园艺系重庆400716

概述了多倍体育种领域中多倍体产生途径，特征、特性及鉴定方法等研究现状。

关键词：多倍体诱导育种植物特征特性鉴定

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园艺植物中三倍体的应用现状及育种前景分析

园艺学报 2018年第9期45卷 1813-1830页

作者：党江波宋琴李彩郭启高梁国鲁西南大学园艺园林学院重庆400716 重庆市涪陵区果品办公室重庆408000

对三倍体在园艺植物中的应用现状进行概述并对其育种前景进行分析。三倍体产生途径中,2n配子途径值得重视,自然条件下或经人工诱导少数植物能高频产生2n配子(超过80%),一些与2n配子产生相关的基因已被鉴定;此外,自然细胞融合、多精受精... 详细信息

对三倍体在园艺植物中的应用现状进行概述并对其育种前景进行分析。三倍体产生途径中,2n配子途径值得重视,自然条件下或经人工诱导少数植物能高频产生2n配子(超过80%),一些与2n配子产生相关的基因已被鉴定;此外,自然细胞融合、多精受精被用于植物三倍体的创制;培养内多倍体(Endopolyploid)细胞再生植株,有助于获得四倍体亲本。在三倍体筛选、鉴定方面,除传统的形态学、细胞学方法外,QF-PCR、SSR、SNP等分子标记也得到应用。目前三倍体已在40多种园艺植物中直接应用,除较为常规的无籽、高产、抗性强等特点外,营养成分含量高、树形矮化是三倍体在园艺植物中应用的新亮点。利用三倍体培育非整倍体对多样化材料的创制有重要意义;值得注意的是,三倍体杂种中亲本之一的基因组仅占1/3,与传统杂交育种结合,可能对缩短育种周期有利。

关键词：三倍体园艺植物无籽非整倍体育种周期

天然四倍体枇杷‘B431’减数分裂观察及育性分析

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园艺学报 2018年第10期45卷 1895-1904页

作者：梁森林党江波梁国鲁郭启高西南大学园艺园林学院重庆400715

通过流式细胞术和染色体制片确定枇杷天然实生筛选株系‘B431’为四倍体。采用改良涂片法观察了其花粉母细胞减数分裂过程,离体萌发法和人工杂交分析其育性,染色体计数法检测了其后代的染色体数。结果表明:天然四倍体枇杷‘B431’减数... 详细信息

通过流式细胞术和染色体制片确定枇杷天然实生筛选株系‘B431’为四倍体。采用改良涂片法观察了其花粉母细胞减数分裂过程,离体萌发法和人工杂交分析其育性,染色体计数法检测了其后代的染色体数。结果表明:天然四倍体枇杷‘B431’减数分裂过程中染色体存在异常行为;染色体配对以二价体为主,也可见少量单价体和部分多价体;减数分裂进程中出现板外染色体、早熟染色体、落后染色体、染色体桥、微核和极少的三极体等异常现象;四分体时期有二分体、三分体、多分体和微核等异常现象。四倍体枇杷‘B431’育性低于二倍体,但其花粉活力达到66.29%±2.22%,杂交结实率与二倍体差异不显著,仍有较高的育性,其与二倍体的杂交后代三倍体率达到66.67%,可以作为三倍体创制的核心亲本。

关键词：枇杷四倍体三倍体减数分裂花粉活力杂交倍性

涪陵磨盘沟自然保护区桫椤种群分布格局的分形特征

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生态学杂志 2005年第5期24卷 581-584页

作者：石胜友郭启高成明昊梁国鲁涪陵师范学院重庆408003 西南农业大学园艺园林学院重庆400716

利用分形理论中的计盒维数探讨了重庆市涪陵磨盘沟桫椤种群的分形特征。结果表明,桫椤种群的分布格局具有分形特征。桫椤种群在不同海拔高度分布格局的计盒维数各不相同,与生境密切相关。在6个不同植物群落中,桫椤种群的计盒维数大小依... 详细信息

利用分形理论中的计盒维数探讨了重庆市涪陵磨盘沟桫椤种群的分形特征。结果表明,桫椤种群的分布格局具有分形特征。桫椤种群在不同海拔高度分布格局的计盒维数各不相同,与生境密切相关。在6个不同植物群落中,桫椤种群的计盒维数大小依次为黄牛奶树淡竹叶>黄杞水麻>盐肤木腹水草>血桐芒>白栎扁穗莎草>毛叶木姜子火炭木。计盒维数定量地反映了该自然保护区桫椤种群占据生态空间的能力,随机型的桫椤种群对空间占有能力比聚集型的低。

关键词：桫椤分形特征计盒维数种群格局群落

三倍体枇杷花期调控相关的EjbHLH79基因序列和表达特性分析

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植物生理学报 2021年第6期57卷 1300-1310页

作者：胡若倩景丹龙郭启高梁国鲁南方山地园艺学教育部重点实验室西南大学南方山地作物逆境生物学国家级培育基地西南大学农业科学研究院园艺园林学院重庆400715 西南大学含弘学院重庆400715

以花蕾露白期的三倍体枇杷(Eriobotrya japonica)‘Q11’为材料,通过同源克隆技术获得bHLH转录因子家族基因EjbHLH79。该基因的编码区序列(CDS)长为867 bp,编码288个氨基酸,蛋白分子质量为31.32 kDa,等电点为8.26。蛋白质序列比对分析表... 详细信息

以花蕾露白期的三倍体枇杷(Eriobotrya japonica)‘Q11’为材料,通过同源克隆技术获得bHLH转录因子家族基因EjbHLH79。该基因的编码区序列(CDS)长为867 bp,编码288个氨基酸,蛋白分子质量为31.32 kDa,等电点为8.26。蛋白质序列比对分析表明,EjbHLH79与梨(Pyrus ussuriensis×***)、桃(Amygdalus persica)等植物的bHLH79蛋白质序列具有较高的同源性,均具有1个保守的碱性(basic)结构域和1个保守的螺旋-环-螺旋(HLH)结构域。系统进化树分析表明,EjbHLH79与梨、桃等蔷薇科植物bHLH79聚为同一分支。亚细胞定位分析显示,EjbHLH79定位在细胞核中,具有典型的bHLH转录因子亚细胞定位特性。通过实时荧光定量PCR分析进一步发现,二倍体和三倍体枇杷早、晚花材料的EjbHLH79表达量存在明显差异,其中在花发育前5个时期的4个材料中EjbHLH79表达量较低,但在花发育后3个时期(小花分化期、花蕾露白期和盛花期)表达量较高,表明EjbHLH79在花发育后期发挥重要作用。本研究为进一步阐明EjbHLH79基因参与三倍体枇杷花期调控提供理论参考。

关键词：三倍体枇杷 EjbHLH79 花期亚细胞定位表达分析

炭疽菌诱导的枇杷病程相关基因EjPR1的克隆及其表达分析

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园艺学报 2021年第2期48卷 367-376页

作者：邹晖赵亮袁婷郭启高吴頔梁国鲁西南大学园艺园林学院南方山地园艺学教育部重点实验室重庆400715 西南大学农业科学研究院西南大学南方山地作物逆境生物学国家级培育基地重庆400715

以炭疽病病原菌(Colletotrichum gloeosporioides)处理的枇杷高抗品种‘Peluches’的叶片为材料,利用RT-PCR和RACE克隆技术,获得枇杷病程相关蛋白1基因的cDNA全长序列,命名为EjPR1。该基因全长为731 bp(GenBank登录号为MN92775),5′端... 详细信息

以炭疽病病原菌(Colletotrichum gloeosporioides)处理的枇杷高抗品种‘Peluches’的叶片为材料,利用RT-PCR和RACE克隆技术,获得枇杷病程相关蛋白1基因的cDNA全长序列,命名为EjPR1。该基因全长为731 bp(GenBank登录号为MN92775),5′端非编码区79 bp,3′端非编码区91 bp。其中ORF阅读框为561 bp,编码186个氨基酸,蛋白质等电点为5.63,分子量为21485.34,其预测结果的理论值与原核表达结果一致。序列分析结果表明,该基因编码的氨基酸序列与其他6种蔷薇科植物PR1蛋白的氨基酸序列相似性在76.41%~94.36%之间。接种炭疽菌后时序表达分析结果表明,该基因在高抗品种‘Peluches’中受病原菌诱导后明显上调表达,在接种后96 h最高,但在高感品种‘森尾早生’中无明显变化,说明EjPR1可能参与了枇杷的抗病防御过程。

关键词：枇杷 EjPR1 基因克隆原核表达表达分析