检索结果-南通市图书馆

Application of Green Fluorescent Protein Gene (gfp) in the Symbiosis between Mesorhizobium Huakuii and Astragalus Sinicus

在线全文

学校读者我要写书评

暂无评论

Science Foundation in China 2002年第2期10卷 41-44页

作者： Zhou Junchu shi qiaojuan Xie Bo(Huazhong Agricultural University, Key Laboratory of Agricultural Microbiology, Wuhan 430070)

Green fluorescent protein (GFP) is a luminescent protein which was first discovered from A equoreavicto-ria[1]. The chromophore of wild type GFP consists of an imidazoione ring formed by cyclization of Ser65, dehydrat... 详细信息

关键词： green fluorescent protein gene (gfp) Mesorhizobiumhuakuii promoter-probe vector

Identification and expression of a gfpK79R mutant of green fluorescent protein

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2002年第7期 79-82页

作者： ZHOU Junchu, shi qiaojuan, MA Lixin and XIE Bo(The Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China)

A mutant strain of E. coli showing strong green fluorescence is obtained during the study of cloning and expression of blue fluorescent protein gene bfp. A recombinant plasmid pHN122 has been isolated. It is demonstrated by enzyme digestion, subcloning and sequence analysis that it contains both of bfp and gfpK79R mutant genes. The results of spectrometric analysis show that GFPK79R expressed by pHN122 is the same as the wild type GFP, but its fluorescent intensity is increased 1.25 - 2.44-fold.

关键词： green fluorescent protein g/pK79R mutant spectrometric characteristics.

半胱氨酰白三烯受体拮抗剂对全脑缺血再灌注慢性损伤的作用

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

浙江大学学报（医学版） 2018年第1期47卷 19-26页

作者：王浩郭红刚楼琦石巧娟浙江省立同德医院神经内科浙江杭州310012 浙江省医学科学院实验动物中心浙江杭州310013

目的:研究半胱氨酰白三烯受体(Cys LTR)拮抗剂普鲁司特和HAMI3379对全脑缺血再灌注慢性损伤的保护作用及相关作用机制。方法:40只体质量为45~65 g的清洁级健康雄性长爪沙鼠分为手术对照组、模型对照组、普鲁司特组和HAMI 3379组,每组10... 详细信息

目的:研究半胱氨酰白三烯受体(Cys LTR)拮抗剂普鲁司特和HAMI3379对全脑缺血再灌注慢性损伤的保护作用及相关作用机制。方法:40只体质量为45~65 g的清洁级健康雄性长爪沙鼠分为手术对照组、模型对照组、普鲁司特组和HAMI 3379组,每组10只。采用结扎双侧颈总动脉10 min再灌注法制作全脑缺血再灌注损伤模型。普鲁司特组和HAMI 3379组于术前30 min和术后30 min、4 h、12 h分别腹腔注射普鲁司特和HAMI 3379,第二天起每天分别给药一次,连续给药5 d。于全脑缺血再灌注24 h和14 d时对各组的神经症状和功能进行评分;尼氏染色法观察再灌注14 d时各组大脑皮层神经元的形态和数量;免疫组织化学染色检测再灌注14 d时各组大脑皮层小胶质细胞和星形胶质细胞激活情况。结果:30只长爪沙鼠中,21只造模成功,其中模型对照组7只,普鲁司特组6只,HAMI 3378组8只。与模型对照组比较,普鲁司特组和HAMI 3379组术后24 h神经症状评分降低(均P0.05);普鲁司特组和HAMI 3379组在这两个时间点的神经功能评分均高于模型对照组(P<0.05或P<0.01)。普鲁司特组和HAMI 3379组大脑皮层神经元损伤较模型对照组减轻,存活神经元密度与模型对照组差异有统计学意义(均P<0.01)。普鲁司特组和HAMI 3379组大脑皮层小胶质细胞和星形胶质细胞增生情况较模型对照组改善(均P<0.01)。结论:普鲁司特和HAMI 3379对长爪沙鼠全脑缺血慢性损伤模型具有较持久的神经保护作用。

关键词：白三烯拮抗剂/药理学半胱氨酸沙丁胺醇/治疗应用脑缺血/病理生理学再灌注损伤/药物作用神经元/药物作用疾病模型,动物沙鼠亚科

Circulating exo-miR-154-5p regulates vascular dementia through endothelial progenitor cell-mediated angiogenesis

在线全文

学校读者我要写书评

暂无评论

Circulating exo-miR-154-5p regulates vascular dementia throu...

A nonalcoholic fatty liver disease cirrhosis model in gerbil:the dynamic relationship between hepatic lipid metabolism and cirrhosis

第十六届中国实验动物科学年会

作者： Xue Han Li Zhou Yu Tu Jiajia Wei Jiajia Zhang Guojun Jiang qiaojuan shi Huazhong Ying Zhejiang Provincial Key Laboratory of Laboratory Animals and Safety Research Hangzhou Medical College School of Pharmaceutical Sciences Zhejiang Chinese Medical University Department of Pharmacy Affifiliated Xiaoshan HospitalHangzhou Normal University

Objective Vascular dementia(VaD) mainly results from cerebral vascular lesions and tissue changes,which contribute to neurodegenerative *** therapeutic approaches to targeting angiogenesis may reduce mortality of *** progenitor cells(EPCs) play a key role in postnatal *** exosomal microRNAs(exo-miRNAs)have been reported to involve in the development of *** present study was designed to investigate whether the expression profifile of the exomiRNAs is signifificantly altered in patients with VaD and to reveal the function of differentially expressed miRNAs and the relevant mechanisms in EPCmediated angiogenesis in VaD rat *** Exosomes isolated from serum of patients with VaD(n=7) and age-matched control subjects(n=7),and miRNA sequencing and bioinformatics analysis found that circulating exosome miRNA-155-5 p,miRNA-154-5 p,miR-132-5 p,and miR-1294 were upregulated in patients with *** expression of miRNA-154-5 p was further verifified to be upregulated in clinical samples(n=23)and 2-vessel occlusioninduced VaD rat model by reverse transcription quantitative PCR(RTqPCR).Notably,miRNA-154-5 p inhibition in bone marrow-EPCs(BMEPCs) from VaD rats improved EPC functions,including tube formation,migration,and adhesion,and elevated concentrations of vascular endothelial growth factor(VEGF) and stromal cell-derived factor-1α(SDF-1α).The mRNA levels of ICAM-1,VCAM-1,and MCP-1 were reduced in miRNA-154-5 p-inhibited *** addition,miRNA-154-5 p inhibition increased the level of superoxide dismutase(SOD),and decreased reactive oxygen species(ROS) in ***2 was chosen as a promising target gene of miR-154-5 p,and miRNA-154-5 p inhibition upregulated the protein expression of ***,upregulation of miR-154-5 p markedly diminished EPC functions and inhibited angiogenesis following EPC transplantation in VaD *** Circulating exo-miR-154-5 p was upregulated in patients with VaD,and miR-154-

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

A nonalcoholic fatty liver disease cirrhosis model in gerbil...

以绿荧光蛋白基因为报告基因的广宿主启动子探针载体的构建和应用

第十五届中国实验动物科学年会

作者： Wei Li Zheng Guan Jean C Brisset qiaojuan shi Qi Lou Yue Ma Su Suriguga Huazhong Ying Xiaoying Sa Zhenwen Chen Wim J Quax Xiaofeng Chu Laboratory Animal Center Zhejiang University Laboratory Animal Center Zhejiang Academy of Medical Sciences Departments of Chemical and Pharmaceutical Biology GUIDEUniversity of Groningen Department of Radiology New York University Departments of Pharmaceutical Technology and Biopharmacy GUIDEUniversity of Groningen Department of Laboratory Animal Science School of Basic Medical ScienceCapital Medical University

Nonalcoholic fatty liver disease(NAFLD) usually takes decades to develop into cirrhosis,which limits the longitudinal study of *** work aims at developing a NAFLD-caused cirrhosis model in gerbil and examining the dynamic relationship between hepatic lipid metabolism and *** fed gerbil a high-fat and high-cholesterol diet(HFHCD) for 24 weeks,and recorded the gerbil’s phenotype at 3,6,9,12,15,18,21,24 *** model’s pathological process,lipid metabolism,oxidative stress,liver collagen deposition and presence of relevant cytokines were tested and evaluated during the full-time frame of disease *** gerbil model can induce non-alcoholic steatohepatitis(NASH) within 9 weeks,and can develop cirrhosis after 21 weeks *** model’s lipids metabolism disorder is accompanied with the liver damage *** the NAFLD progression,triglycerides(TG) and free fatty acids(FFA) have presented distinct rise and fall tendency,and the turning points are at the fibrosis *** that,the ratios of total cholesterol(CHO) to high-density lipoprotein cholesterol(HDL-C) exhibited constant growth tendency,and have a good linear relationship with HSC(R2 = 0.802,P < 0.001).The gerbil NAFLD cirrhosis model has been developed and possesses positive correlation between lipids metabolism and *** compelling rise and fall tendency of TG and FFA indicated that the fibrosis progression can lead to impairment in lipoprotein synthesis and engender decreased TG ***/HDL-C ratios can imply the fibrosis progress and be used as a blood indicator for disease prediction and prevention.

关键词： Cirrhosis fibrosis hepatic lipid metabolism nonalcoholic fatty liver disease(NAFLD) gerbil

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

微生物学报 1999年第5期39卷 408-415页

作者：马立新史巧娟周俊初陈华癸湖北大学生命科学院农业部农业微生物重点实验室华中农业大学武汉430070

将以绿荧光蛋白基因（ｇｆｐ）的ｃＤＮＡ为模板，用人工合成引物经ＰＣＲ扩增获得的０－９ｋｂＤＮＡ片段克隆到表达载体ｐＥＴ１１Ｃ上构建成ｇｆｐ表达载体ｐＨＮ１１５。从ｐＨＮ１１５上切下的不含启动子，但保留了ＳＤ... 详细信息

将以绿荧光蛋白基因（ｇｆｐ）的ｃＤＮＡ为模板，用人工合成引物经ＰＣＲ扩增获得的０－９ｋｂＤＮＡ片段克隆到表达载体ｐＥＴ１１Ｃ上构建成ｇｆｐ表达载体ｐＨＮ１１５。从ｐＨＮ１１５上切下的不含启动子，但保留了ＳＤ序列的ｇｆｐ基因经克隆载体ＳＫ（＋）和ｐＩＪ２９２５亚克隆后再克隆到广谱稳定性质粒ｐＴＲ１０２上构建成广谱、稳定、可视的启动子探针载体ｐＨＮ１２７。并用它从费氏中华根瘤菌ＨＮ０１的总ＤＮＡ中成功地钓出组成型和诱导型表达的启动子。

关键词：绿荧光蛋白基因中华根瘤菌启动子探针载体

44代Z:ZCLA长爪沙鼠生长繁殖性能的研究

在线全文

学校读者我要写书评

暂无评论

医学研究杂志 2009年第7期38卷 58-61页

作者：周文伟石巧娟施张奎萨晓婴浙江省医学科学院杭州310013

目的通过对第44代Z:ZCLA长爪沙鼠生长繁殖性能的研究与分析,为长爪沙鼠在生物医学领域的研究和应用提供科学依据。对实验动物的开发和应用具有指导意义。方法选择繁殖性能优良,毛色具有光泽,体质健壮的亲本所产的仔鼠200只,雌雄各半,在... 详细信息

目的通过对第44代Z:ZCLA长爪沙鼠生长繁殖性能的研究与分析,为长爪沙鼠在生物医学领域的研究和应用提供科学依据。对实验动物的开发和应用具有指导意义。方法选择繁殖性能优良,毛色具有光泽,体质健壮的亲本所产的仔鼠200只,雌雄各半,在2.5～3月龄采用随机交配的一雌一雄长期同居法配种饲养。结果结果表明:每胎产仔数最少的1只,最多达14只,累计生产了455胎,生产仔数为3191只,每胎平均7.01只,每胎产仔数大多在5～9只,占总胎数的79.34%;观察100只母鼠不同生产胎次的间隔,胎间隔最短20天,最长127天,胎间隔大多在20～60天之间,占总数的72.16%。结论长爪沙鼠从出生到4月龄体重、体长、尾长的增长迅速,在离乳前雌、雄差异不明显,离乳后的整个发育期,雄鼠的体重、体长、尾长均大于雌鼠。

关键词： 44代Z ZCLA长爪沙鼠生长繁殖体重

44代Z:ZCLA长爪沙鼠不同生长时期血清中抗氧化性能研究

在线全文

学校读者我要写书评

暂无评论

医学研究杂志 2009年第6期38卷 30-32页

作者：周文伟石巧娟施张奎萨晓婴浙江省医学科学院杭州310013

目的探讨第44代Z:ZCLA长爪沙鼠在不同生长时期血清中抗氧化作用。方法选择1月龄、3月龄、24月龄的44代Z:ZCLA长爪沙鼠各16只(雌雄各半),测定44代Z:ZCLA长爪沙鼠血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GsH-Px)的... 详细信息

目的探讨第44代Z:ZCLA长爪沙鼠在不同生长时期血清中抗氧化作用。方法选择1月龄、3月龄、24月龄的44代Z:ZCLA长爪沙鼠各16只(雌雄各半),测定44代Z:ZCLA长爪沙鼠血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GsH-Px)的含量变化。结果丙二醛(MDA)在3月龄最低,谷胱甘肽过氧化物酶(GsH-Px)在3月龄最高;超氧化物歧化酶(SOD)随着长爪沙鼠的月龄增长而升高。结论在44代Z:ZCLA长爪沙鼠在不同的生长过程中丙二醛(MDA)含量变化与谷胱甘肽过氧化物酶(GsH-Px)的含量变化有直接关系,而超氧化物歧化酶(SOD)含量变化与MDA含量变化没有直接关系。

关键词： 44代Z ZCLA长爪沙鼠抗氧化性能丙二醛超氧化物歧化酶谷胱甘肽过氧化酶

三羟异黄酮对去卵巢大鼠学习记忆及海马神经元细胞凋亡的影响

在线全文

学校读者我要写书评

暂无评论

浙江中西医结合杂志 2011年第3期21卷 153-155,158页

作者：姚守珍胡志英石巧鹃浙江省中西医结合医院妇产科杭州310003 浙江省医学科学院实验动物中心

目的:观察三羟异黄酮(GST)对去卵巢大鼠学习记忆能力及海马神经元细胞凋亡影响,探讨植物雌激素对中枢神经系统的保护作用及可能作用机制。方法:选取成熟雌性清洁级大鼠50只,随机分为假手术组,去卵巢对照组,GST低、中、高剂量组,每组10... 详细信息

目的:观察三羟异黄酮(GST)对去卵巢大鼠学习记忆能力及海马神经元细胞凋亡影响,探讨植物雌激素对中枢神经系统的保护作用及可能作用机制。方法:选取成熟雌性清洁级大鼠50只,随机分为假手术组,去卵巢对照组,GST低、中、高剂量组,每组10只。术后1周,GST低、中、高剂量组大鼠每天灌胃以二甲基亚砜为溶媒的GST溶液(剂量分别为25、50、100mg/kg体质量);去卵巢对照组和假手术组大鼠每天灌胃与受试药物等容积的溶媒二甲基亚砜。实验持续4周。水迷宫实验观察各组大鼠学习记忆能力,流式细胞液检测大鼠海马神经元凋亡;结果:与假手术比较,去卵巢对照组大鼠水迷宫学习记忆能力潜伏期明显延长(P0.05);去卵巢对照组海马内Annexin V含量较假手术对照组明显升高(P0.05)。结论:三羟异黄酮能明显改善去卵巢大鼠学习记忆能力,其作用机制可能与抑制去卵巢大鼠海马神经元细胞凋亡有关。

关键词：大鼠学习记忆海马细胞凋亡 Annexin V 三羟异黄酮