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羟基喜树碱/GA-PEI-PLGA纳米粒的制备及其工艺优化

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医药导报 2016年第11期35卷 1254-1258页

作者：梁劲康吴得天胡巧红吴雅琼广东药科大学药学院广州510006 广东药科大学广东省药物新剂型重点实验室广州510006

目的制备包载羟基喜树碱(HCPT)的甘草次酸修饰的PEI-PLGA(GA-PEI-PLGA)纳米粒。方法采用超声乳化-减压溶剂挥发法制备HCPT/GA-PEI-PLGA纳米粒。在单因素实验基础上,选取药物/载体比、油相/水相体积比、超声时间、超声功率为考察因素,以... 详细信息

目的制备包载羟基喜树碱(HCPT)的甘草次酸修饰的PEI-PLGA(GA-PEI-PLGA)纳米粒。方法采用超声乳化-减压溶剂挥发法制备HCPT/GA-PEI-PLGA纳米粒。在单因素实验基础上,选取药物/载体比、油相/水相体积比、超声时间、超声功率为考察因素,以包封率和载药量为考察指标,通过正交设计实验对载药纳米粒的制备工艺进行优化,并对其粒径及Zeta电位进行测定。结果超声乳化-减压溶剂挥发法成功制得HCPT/GA-PEI-PLGA纳米粒,其中药物/载体比对载药纳米粒制备的影响最大。优化后的处方工艺为:药物/载体比3∶10、油相/水相体积比1∶9、超声功率570 W、超声时间15 min。制得的载药纳米粒包封率高达(87.52±3.91)%,载药量为(20.10±4.72)%;平均粒径(218.1±3.23)nm,Zeta电位为(34.98±3.56)m V。结论超声乳化-减压溶剂挥发法适于制备HCPT/GA-PEI-PLGA纳米粒,制得的载药纳米粒包封率和载药量较高、粒径分布均匀、稳定性好。

关键词：羟基喜树碱/GA-PEI-PLGA纳米粒正交设计实验制备工艺

初中学生人乳头瘤病毒及其疫苗知识的知晓水平和疫苗接种意愿的影响因素

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国际流行病学传染病学杂志 2024年第3期51卷 193-198页

作者：赵湘黄玉吕巧红吴青青浙江省疾病预防控制中心健康教育所杭州310051

目的了解知晓HPV疫苗的初中学生对HPV及相关疫苗知识的知晓情况, 及其对该疫苗的接种意愿, 为在该人群中推广使用HPV疫苗, 预防HPV感染相关疾病提供科学依据。方法 2023年3—5月, 在抽取的浙江省3个城市6个县区6所初中学校中开展在线匿... 详细信息

目的了解知晓HPV疫苗的初中学生对HPV及相关疫苗知识的知晓情况, 及其对该疫苗的接种意愿, 为在该人群中推广使用HPV疫苗, 预防HPV感染相关疾病提供科学依据。方法 2023年3—5月, 在抽取的浙江省3个城市6个县区6所初中学校中开展在线匿名自填式问卷调查。调查内容包括人口统计学特征、HPV知识、HPV疫苗知识、感染风险及HPV预防教育。采用Logistic回归分析初中学生HPV疫苗接种意愿相关因素。结果共调查学生4 089人, 1 551人报告知晓HPV疫苗, 知晓率为37.9%。在知晓HPV疫苗的学生中, 1 352名表示愿意接种HPV疫苗, 199名不愿意接种。多因素分析结果显示, 女生、知晓周围有人接种过HPV疫苗、接种HPV疫苗效果测量得分分值高, 认为初中学校有必要开展HPV和HPV疫苗相关的健康教育、HPV感染后果测量得分5~7分是愿意接种HPV疫苗的促进因素。结论浙江省初中学生对HPV疫苗的知晓率低, 知晓HPV疫苗的初中学生HPV接种意愿虽然较高, 但HPV感染后果、HPV疫苗疾病预防知识水平有待提高。

关键词：人乳头瘤病毒疫苗接种意愿影响因素知晓率

老年妇科恶性肿瘤患者化疗相关性恶心呕吐与癌因性疲乏的关系及其影响因素

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中华老年多器官疾病杂志 2024年第4期23卷 276-280页

作者：杜巧红廉莉吴瑞丽运城市中心医院放射治疗科山西运城044000 运城市中心医院乳腺科山西运城044000 运城市中心医院健康教育科山西运城044000

目的分析老年妇科恶性肿瘤患者化疗相关性恶心呕吐(CINV)现状、影响因素及其与癌因性疲乏(CRF)的关系。方法选取2021年3月至2022年9月运城市中心医院乳腺科收治的206例老年妇科恶性肿瘤化疗患者为研究对象。根据CINV发生情况,将患者分为... 详细信息

目的分析老年妇科恶性肿瘤患者化疗相关性恶心呕吐(CINV)现状、影响因素及其与癌因性疲乏(CRF)的关系。方法选取2021年3月至2022年9月运城市中心医院乳腺科收治的206例老年妇科恶性肿瘤化疗患者为研究对象。根据CINV发生情况,将患者分为CINV组92例及非CINV组114例。收集患者临床资料。采用多维疲劳量表(MFI-20)评估患者CRF情况,并分析其与CINV的关系。采用SPSS 22.0统计软件进行数据分析。根据数据类型,组间比较采用独立样本t检验、χ^(2)检验或秩和检验。采用logistic回归模型分析影响老年妇科恶性肿瘤患者CINV的危险因素。结果206例患者中,92例(44.66%)发生CINV。其中,52例(56.52%)为急性CINV,40例(43.48%)为延迟性CINV;34例(36.96%)为轻度CINV,35例(38.04%)为中度CINV,23例(25.00%)为重度CINV。多因素logistic回归分析显示,胃部疾病史(OR=2.438,95%CI 1.106~5.372)、晕车史(OR=3.051,95%CI 1.460~6.376)、含顺铂化疗方案(OR=3.678,95%CI 1.519~8.911)、焦虑(OR=2.542,95%CI 1.174~5.508)、抑郁(OR=2.333,95%CI 1.098~4.959)及CRF(OR=2.769,95%CI 1.232~6.221)均为CINV的独立危险因素(均P<0.05)。不同CINV程度患者的CRF程度、MFI-20评分比较,差异均有统计学意义(P<0.05)。结论有胃部疾病史、有晕车史、含顺铂化疗方案、焦虑、抑郁及CRF均为CINV的独立危险因素,其中CRF程度与CINV程度密切相关。

关键词：老年人妇科恶性肿瘤化疗相关性恶心呕吐危险因素

连续性肾脏替代治疗管路寿命风险预测模型的研究进展

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中华急危重症护理杂志 2024年第2期5卷 188-192页

作者：杨二明王巧红毛娅吴燕铭杨辉山西医科大学护理学院太原市030000 山西医科大学第一医院重症医学科山西医科大学第一医院护理部

连续性肾脏替代治疗是重症患者重要的生命支持技术,但其管路寿命过早衰竭严重降低了患者治疗的充分性。该文综述了连续性肾脏替代治疗管路寿命风险预测模型的适用人群、预测因子、结局指标、预测性能以及对临床实践的意义。连续性肾脏... 详细信息

连续性肾脏替代治疗是重症患者重要的生命支持技术,但其管路寿命过早衰竭严重降低了患者治疗的充分性。该文综述了连续性肾脏替代治疗管路寿命风险预测模型的适用人群、预测因子、结局指标、预测性能以及对临床实践的意义。连续性肾脏替代治疗管路寿命的风险预测模型各有侧重,泛化能力有待验证,医护人员在应用时需甄别模型适用范围与预测性能。

关键词：连续性肾脏替代疗法管路寿命模型,统计学质量控制综述文献(主题)

Effects of sediment dredging on water quality and zooplankton community structure in a shallow of eutrophic lake

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Journal of Environmental Sciences 2010年第2期22卷 218-224页

作者： Shiyang Zhang qiaohong Zhou Dong Xu Jidong Lin Shuiping Cheng Zhenbin wu State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of Chinese Academy of Sciences Beijing 100049 China wuhan Water Authority Wuhan 430014 China

Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, total suspended solids, Chlorophyll a and Secchi transparency in the water column was found, while levels of water depth, electrical conductivity, total dissolved solids and NO3^--N concentration increased markedly post-dredging. The effects of dredging on dissolved oxygen, pH value and temperature were almost negligible. The zooplankton community structure responded rapidly to the environmental changes caused mainly by dredging. As a result, the abundance of rotifers decreased, while the density of zooplanktonic crustaceans increased markedly. The representative taxa were Brachionus angularis, B. budapestinensis, B. diversicornis, Synchaeta spp. and Neodiaptomus schmackeri. A distinct relationship between zooplankton taxa composition and their environment, unraveled by a redundancy analysis, indicating that the measured environment contributed to the variations in the zooplankton community structure to some extent. The first four synthetic environmental variables explained 51.7% of the taxonomic structure. Therefore, with the reduction of internal nutrient load and a shift in dominance by less eutrophic species, it inferred that dredging might be one of effective measures for environmental improvements of such lakes.

关键词： zooplankton community structure redundancy analysis internal nutrient load

Comparative study of microbial community structure in different filter media of constructed wetland

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Journal of Environmental Sciences 2010年第1期22卷 127-133页

作者： Ming Li qiaohong Zhou Min Tao Ying Wang Lijuan Jiang Zhenbin wu State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate University of Chinese Academy of Sciences Beijing 100039 China School of Resource & Environmental Engineering Wuhan University of Technology Wuhan 430070 China

Comparisons of microbial community structure, in eight filter media of zeolites, anthracite, shale, vermiculite, ceramic filter media, gravel, steel slag and bio-ceramic, were undertaken by analyzing the phospholipid fatty acid （PLFA） composition. A total of 20 fatty acids in the range of C18to C20 were determined but only 13 PLFAs were detected in steel slag. They consist of saturated fatty acids, branched fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids. The variation of fatty acids was revealed in the relative proportions of these fatty acids in different media. The aerobic prokaryotes were the predominant group in all media. The PLFA composition showed significant differences among the eight different media by Tukey＇s honestly test. It was found that steel slag was significantly different in the microbial community as compared to other filter media, probably due to its alkaline effluent. Steel slag alone is probably not a good choice of substratum in constructed wetlands. The principle components analysis （PCA） showed that zeolites, bio-ceramic, shale and vermiculite had a similar microbial community structure while steel slag and ceramic filter media were distinct from other media.

关键词： microbial community structure phospholipid filter media biomarkers constructed wetland steel slag

闽东地区畲族成年人H型高血压与亚甲基四氢叶酸还原酶基因 C677T多态性的关联

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中华高血压杂志 2023年第7期31卷 649-654页

作者：王志刚吴巧红黄声曾茜刘晓斌苏明宽朱雁洲范金茂福建医科大学附属闽东医院老年医学科福建福安355000 福建医科大学附属闽东医院检验科福建福安355000

目的探讨闽东地区畲族成年人H型高血压与亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性的关联性。方法选取福建省宁德市闽东医院2019年10月至2022年8月在高血压门诊就诊、住院和进行健康体检,且三代内未与其他民族通婚、居住在福安市境... 详细信息

目的探讨闽东地区畲族成年人H型高血压与亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性的关联性。方法选取福建省宁德市闽东医院2019年10月至2022年8月在高血压门诊就诊、住院和进行健康体检,且三代内未与其他民族通婚、居住在福安市境内的畲族成年人共309人为受试者。根据血压和同型半胱氨酸(Hcy)水平分为4组:Hcy正常非高血压组58例;Hcy升高非高血压组59例;Hcy正常高血压组77例;H型高血压组115例。Hcy≥10μmol/L定义为升高。采用Sanger测序法检测MTHFR基因C677T多态性,生化分析仪测定Hcy水平。比较4组患者MTHFR基因C677T多态性的差异,通过logistic回归分析MTHFR C677T基因多态性与H型高血压的关联。结果Hcy正常非高血压组中MTHFR C677T基因CC、CT、TT基因型频率分别为70.7%、25.9%、3.4%;Hcy升高非高血压组中分别为54.2%、42.4%、3.4%;Hcy正常高血压组中分别为51.9%、41.6%、6.5%;H型高血压组中分别为36.5%、47.8%、15.7%。H型高血压组CT、TT基因型频率高于其他3组,而CC基因型频率低于其他3组(χ^(2)=24.790,P<0.001);其他3组基因型频率差异无统计学意义(χ^(2)=6.004,P=0.199)。H型高血压组不同基因型患者Hcy差异有统计学意义[TT基因型21.15(14.22~28.40)比CT基因型15.10(12.00~17.30)比CC基因型15.40(12.75~16.60)μmol/L,Z=9.866,P=0.007]。多因素logistic回归分析显示,MTHFR基因C677T TT基因型发生H型高血压的风险增加[OR(95%CI)=2.666(1.719~4.134)]。结论闽东地区畲族成年人H型高血压与MTHFR基因C677T多态性可能存在关联。

关键词：畲族宁德市亚甲基四氢叶酸还原酶基因 C677T 同型半胱氨酸 H型高血压

Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater

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Journal of Environmental Sciences 2008年第10期20卷 1210-1217页

作者： DU, Cheng wu, Zhenbin XIAO, Enrong ZHOU, qiaohong CHENG, Shuiping LIANG, Wei HE, Feng State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate University Chinese Academy of Sciences Beijing 100039 China

The bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCBI for identification purposes. The 90.6% of the clones were a?l...

关键词： bacterial diversity restriction fragment length polymorphism (RFLP) submerged membrane bioreactor (SMBR) 16S rDNA clone library

Structural and metabolic responses of microbial community to sewage-borne chlorpyrifos in constructed wetlands

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Journal of Environmental Sciences 2016年第6期28卷 4-12页

作者： Dan Zhang Chuan Wang Liping Zhang Dong Xu Biyun Liu qiaohong Zhou Zhenbin wu State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

Long-term use of chlorpyrifos poses a potential threat to the environment that cannot be ignored, yet little is known about the succession of substrate microbial communities in constructed wetlands（CWs） under chlorpyrifos stress. Six pilot-scale CW systems receiving artificial wastewater containing 1 mg/L chlorpyrifos were established to investigate the effects of chlorpyrifos and wetland vegetation on the microbial metabolism pattern of carbon sources and community structure, using BIOLOG and denaturing gradient gel electrophoresis（DGGE） approaches. Based on our samples, BIOLOG showed that Shannon diversity（HV） and richness（S） values distinctly increased after 30 days when chlorpyrifos was added. At the same time, differences between the vegetated and the non-vegetated systems disappeared. DGGE profiles indicated that H Vand S had no significant differences among four different treatments. The effect of chlorpyrifos on the microbial community was mainly reflected at the physiological level. Principal component analysis（PCA） of both BIOLOG and DGGE showed that added chlorpyrifos made a difference on test ***, there was no difference between the vegetation and no-vegetation treatments after addition of chlorpyrifos at the physiological level. Moreover, the vegetation had no significant effect on the microbial community at the genetic level. Comparisons were made between bacteria in this experiment and other known chlorpyrifos-degrading bacteria. The potential chlorpyrifos-degrading ability of bacteria in situ may be considerable.

关键词： Constructed wetland Chlorpyrifos BIOLOG DGGE