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不同饵料对中华绒螯蟹幼体发育和存活的影响

水产学报 2000年第5期24卷 442-447页

作者：江洪波陈立侨周忠良陈明朱钧华东师范大学生物系!上海200062 上海浦东孙桥名特水产开发有限公司!上海2001203

采用高度不饱和脂肪酸（HUFA）营养强化的轮虫、轮虫与卤虫组合投喂中华绒螯蟹幼体 ,以各期幼体的存活率为评判指标 ,对不同饵料及其组合的投喂效果进行了探讨。研究结果表明 :轮虫是中华绒螯蟹早期幼体（Ⅰ、Ⅱ期）的适口饵料 ,幼... 详细信息

采用高度不饱和脂肪酸（HUFA）营养强化的轮虫、轮虫与卤虫组合投喂中华绒螯蟹幼体 ,以各期幼体的存活率为评判指标 ,对不同饵料及其组合的投喂效果进行了探讨。研究结果表明 :轮虫是中华绒螯蟹早期幼体（Ⅰ、Ⅱ期）的适口饵料 ,幼体存活率随轮虫密度的增加而逐渐上升 ,但当轮虫数量超过最适密度时 ,幼体存活率反而有所下降。其中 ,未强化的轮虫最适投喂密度为 6 0ind·mL-1,强化轮虫最适密度为40ind·mL-1。状Ⅰ、Ⅱ期投喂 40ind·mL-1轮虫 ,从Ⅲ期开始投喂 10ind·mL-1卤虫无节幼体 ,能较好地满足中华绒螯蟹幼体发育的营养需求 ,提高大眼幼体的存活率。表明强化幼体饵料HUFA特别是EPA和DHA能有效地促进幼体的发育与存活和脱壳率

关键词：中华绒螯蟹幼体发育存活率饵料

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花椒叶在椒盐曲奇饼干制作工艺中的应用研究

食品研究与开发 2018年第14期39卷 103-109页

作者：张欢梁晶晶刘阳乔明锋胡军姜元华四川旅游学院四川成都610100 中国科学院山地生态恢复与生物资源利用重点实验室生态恢复与生物多样性保育四川省重点实验室中国科学院成都生物研究所四川成都610041

以汉源花椒的副产品花椒叶作为原料,感官评分作为依据,先后在原料配方,工艺方案上采用单因素和正交试验,探讨影响花椒叶椒盐曲奇饼干感官评分值的因素,最后采用质构分析,用科学理论的方法确定感官评分值。最终确定花椒叶椒盐曲奇饼干配... 详细信息

以汉源花椒的副产品花椒叶作为原料,感官评分作为依据,先后在原料配方,工艺方案上采用单因素和正交试验,探讨影响花椒叶椒盐曲奇饼干感官评分值的因素,最后采用质构分析,用科学理论的方法确定感官评分值。最终确定花椒叶椒盐曲奇饼干配方为:低筋面粉100%,黄油70%,食盐2.7%,花椒叶粉1.5%,糖粉20%,水15%。工艺优化最佳结果为搅拌时间8 min,烘烤时间13 mim,上火温度180℃,下火温度150℃。

关键词：花椒叶曲奇饼干单因素正交质构

以市场需求为导向的企业技术研发方向识别研究

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创新科技 2024年第7期24卷 63-74,F0002页

作者：张硕乔亚丽李荣荣北京工业大学经济与管理学院北京100124 清华大学公共管理学院北京100084 中国石油大学(华东)经济管理学院山东青岛266580

识别领域内重点技术研发方向,是企业技术创新活动中不可或缺的关键环节,不仅关乎资源优化配置和创新效率提升,而且是推动企业持续发展、实现技术突破的重要战略举措。面向市场(或用户)需求,选择从竞争者导向角度出发,构建企业技术研发... 详细信息

识别领域内重点技术研发方向,是企业技术创新活动中不可或缺的关键环节,不仅关乎资源优化配置和创新效率提升,而且是推动企业持续发展、实现技术突破的重要战略举措。面向市场(或用户)需求,选择从竞争者导向角度出发,构建企业技术研发方向识别模型。首先,利用文本挖掘技术对领域内相关专利文本进行SAO语义结构提取和专利权人提取,并以与技术相关的S或O概念层级实体为技术表征,构建企业—技术关联网络及企业合作网络;其次,将企业—技术关联矩阵进行z-score标准化得到企业—技术评分矩阵,并将社团划分算法应用于企业合作网络,综合社团属性和企业相似性分值选取Top-k项企业所涉及的技术,形成候选技术列表;最后,构建技术增长性和技术适用性指标,对识别出的候选技术进行定量评估,并结合企业自身技术布局为其推荐适合的发展方向。实证研究部分,以默沙东公司(MERI-C)为例,进行技术研发方向识别研究,为企业精准高效开展技术创新活动提供科学的决策依据。

关键词：技术机会 SAO语义挖掘协同过滤算法技术研发方向

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Ovalbumin-stabilized gold nanoclusters with ascorbic acid as reducing agent for detection of serum copper

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Chinese Chemical Letters 2018年第3期29卷 366-370页

作者： Yifan Chen Juan qiao Qianrong Liu Li Qi Beijing National Laboratory of Molecular Sciences Key Laboratory of Analytical Chemistry for Living Biosystems Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China University of-Chinese Academy of Sciences Beijing 100049 China zhuhai College of Jilin University Zhuhai 519041 China Xi'an University of Science and Technology Xi'an 710054 China

Ovalbumin-stabilized gold nanoclusters （OVA@AuNCs） were prepared with ascorbic acid as a reducing agent. This strategy could realize the synthesis of water-soluble OVA@AuNCs within 20 min. The asprepared fluorescent probe showed a red fluorescence emission at 630 nm. Moreover, the properties of the OVA@AuNCs were characterized by transmission electron microscope, dynamic light scattering, ultraviolet-visible spectroscopy, fluorescent spectroscopy. Based on the surface electron density decrease-induced fluorescence quenching mechanism, the OVA@AuNCs provided high sensitivity and selectivity for sensing copper ions. A good linear relationship was obtained between the fluorescence intensity of OVA@AuNCs and the concentration of copper ions in the range of 5.0-100.0pumol/L （R2z0.999） with a detection limit of 640 nmol/L. Furthermore, the rat serum copper contents were determined by using the OVA@AuNCs based assay, indicating great potential of fluorescent probes for application in biological and clinical analysis.

关键词： Ovalbumin-stabilized gold nanoclusters Fluorescent probe Ascorbic acid Reducing agent Serum copper

清热解毒汤治疗哺乳期急性乳腺炎的回顾性研究

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浙江中医药大学学报 2024年第1期48卷 67-76页

作者：谢晓晨陈雯黄巧张董晓首都医科大学附属北京中医医院北京100010 北京中医药大学

[目的]探讨清热解毒汤治疗哺乳期急性乳腺炎的临床疗效。[方法]回顾性分析2019年1月至2020年12月就诊于首都医科大学附属北京中医医院乳腺科的哺乳期乳腺炎患者的临床资料,其中治疗组80例,内服清热解毒汤;对照组90例,无内服汤药。观察... 详细信息

[目的]探讨清热解毒汤治疗哺乳期急性乳腺炎的临床疗效。[方法]回顾性分析2019年1月至2020年12月就诊于首都医科大学附属北京中医医院乳腺科的哺乳期乳腺炎患者的临床资料,其中治疗组80例,内服清热解毒汤;对照组90例,无内服汤药。观察两组治疗前后乳腺炎的严重程度指数、疾病改善率、体温、乳房疼痛评分、乳汁通畅情况、乳头皲裂情况、中医全身症状评分等指标。[结果]在乳腺炎的严重程度指数、疾病改善率、乳房疼痛评分、乳汁通畅情况、中医全身症状方面组间差异有统计学意义(P<0.05),治疗组疗效优于对照组。[结论]清热解毒汤可有效改善哺乳期急性乳腺炎疼痛、肿块、红肿、乳汁堵塞等情况,疗效确切。

关键词：清热解毒汤哺乳期急性乳腺炎临床研究疼痛肿块疾病改善率

Effect of NaCl treatments on glucosinolate metabolism in broccoli sprouts

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Journal of zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第2期14卷 124-131页

作者： Rong-fang GUO Gao-feng YUAN qiao-mei WAng Department of Horticulture Zhejiang University School of Food and Pharmacy Zhejiang Ocean University Key Laboratory of Horticultural Plant Growth Development and Quality ImprovementMinistry of Agriculture

To understand the regulation mechanism of NaCI on glucosinolate metabolism in broccoli sprouts, the germination rate, fresh weight, contents of glucosinolates and sulforaphane, as well as myrosinase activity of broccoli sprouts germinated under 0, 20, 40, 60, 80, and 100 mmol/L of NaCI were investigated in our experiment. The results showed that glucoerucin, glucobrassicin, and 4-hydroxy glucobrassicin in 7-d-old broccoli sprouts were significantly enhanced and the activity of myrosinase was inhibited by 100 mmol/L of NaCI. However, the total glucosinolate content in 7-d-old broccoli sprouts was markedly decreased although the fresh weight was significantly increased after treatment with NaCI at relatively low concentrations （20, 40, and 60 mmol/L）. NaCI treatment at the concentration of 60 mmol/L for 5 d maintained higher biomass and comparatively higher content of glucosinolates in sprouts of broccoli with decreased myrosinase activity. A relatively high level of NaCI treatment （100 mmol/L） significantly increased the content of sulforaphane in 7-d-old broccoli sprouts compared with the control. These results indicate that broccoli sprouts grown under a suitable concentration of NaCI could be desirable for human nutrition.

关键词： Glucosinolates Brassica oleracea Sulforaphane Myrosinase NaCI

Effect of epidermal growth factor on follicle-stimulating hormone-induced proliferation of granulosa cells from chicken prehierarchical follicles

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Journal of zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第11期12卷 875-883页

作者： Jin-xing LIN Yu-dong JIA Cai-qiao zhAng Key Laboratory of Animal Epidemic Etiology ＆ Immunological Prevention of the Ministry of Agriculture Department of Veterinary Medicine College of Animal Sciences Zhejiang University Hangzhou 310058 China

The development of ovarian follicular cells is controlled by multiple circulating and local hormones and factors, including follicle-stimulating hormone （FSH） and epidermal growth factor （EGF）. In this study, the stagespecific effect of EGF on FSH-induced proliferation of granulosa cells was evaluated in the ovarian follicles of egg-laying chickens. Results showed that EGF and its receptor （EGFR） mRNAs displayed a high expression in granulosa cells from the prehierarchical follicles, including the large white follicle （LWF） and small yellow follicle （SYF）, and thereafter the expression decreased markedly to the stage of the largest preovulatory follicle. SYF represents a turning point of EGF/EGFR mRNA expression during follicle selection. Subsequently the granulosa cells from SYF were cultured to reveal the mediation of EGF in FSH action. Cell proliferation was remarkably increased by treatment with either EGF or FSH （0.1-100 ng/ml）. This result was confirmed by elevated proliferating cell nuclear antigen （PCNA） expression and decreased cell apoptosis. Furthermore, EGF-induced cell proliferation was accompanied by increased mRNA expressions of EGFR, FSH receptor, and the cell cycle-regulating genes （cyclins D1 and El, cyclin-dependent kinases 2 and 6） as well as decreased expression of luteinizing hormone receptor mRNA. However, the EGF or FSH-elicited effect was reversed by simultaneous treatment with an EGFR inhibitor AG1478. In conclusion, EGF and EGFR expressions manifested stage-specific changes during follicular development and EGF mediated FSH-induced cell proliferation and retarded cell differentiation in the prehierarchical follicles. These expressions thus stimulated follicular growth before selection in the egg-laying chicken.

关键词： Epidermal growth factor Follicle-stimulating hormone Granulosa cell Proliferation Chicken

XAF1 is frequently methylated in human esophageal cancer

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World Journal of Gastroenterology 2012年第22期18卷 2844-2849页

作者： Xiang-Yu Chen qiao-Yu He Ming-zhou Guo Department of Gastroenterology The First Affiliated Hospital of Zhengzhou University Zheng-zhou 450052 Henan Province China Department of Gastroenterology and Hepatol-ogy Chinese PLA General Hospital 13eijing 100853 China

AIM： To explore epigenetic changes in the gene encod- ing X chromosome-linked inhibitor of apoptosis-associ- ated factor 1 （XAF1） during esophageal carcinogenesis. METHODS： Methylation status of XAF1 was detected by methylation-specific polymerase chain reaction （MSP） in four esophageal cancer cell lines （KYSE30, KYSE70, BICl and partially methylated in TE3 cell lines）, nine cases of normal mucosa, 72 cases of pri- mary esophageal cancer and matched adjacent tissue. XAF1 expression was examined by semi-quantitative reverse transcriptional polymerase chain reaction and Western blotting before and after treatment with 5-aza- deoxycytidine （5-aza-dc）, a demethylating agent. To investigate the correlation of XAF1 expression and methylation status in primary esophageal cancer, immu- nohistochemistry for XAF1 expression was performed in 32 cases of esophageal cancer and matched adjacent tissue. The association of methylation status and clini-copathological data was analyzed by logistic regression. RESULTS： MSP results were as follows： loss of XAF1 expression was found in three of four esophageal cell lines with promoter region hypermethylation （com- pletely methylated in KYSE30, KYSE70 and BIC1 cell lines and partially in TE3 cells）; all nine cases of normal esophageal mucosa were unmethylated; and 54/72 （75.00%） samples from patients with esophageal can- cer were methylated, and 25/72 （34.70%） matched adjacent tissues were methylated （75.00% vs 34,70%, z2 = 23.5840, P = 0.000）. mRNA level of XAF1 mea- sured with semi-quantitative reverse transcription poly- merase chain reaction was detectable only in TE3 cells, and no expression was detected in KYSE30, KYSE70 or BIC1 cells. Protein expression was not observed in KYSE30 cells by Western blotting before treatment with 5-aza-dc. After treatment, mRNA level of XAF1 was detectable in KYSE30, KYSE70 and BIC1 cells. Protein expression was detected in KYSE30 after treatment with 5-aza-dc. Immunohistochemistry was performed on 3

关键词： X chromosome-linked inhibitor of apoptosis-associated factor 1 Esophageal cancer Methylation Methylation-specific polymerase chain reaction Semi-quantitative reverse transcriptional polymerase chainreaction

腹腔镜近端胃切除后Kamikawa吻合术与双通道吻合术的近期疗效的倾向性评分匹配分析

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中华胃肠外科杂志 2024年第3期27卷 261-267页

作者：杨海成贺加星杨莹韩卓张波周帅吴涛乔庆何显力王楠空军军医大学唐都医院普通外科西安710038

目的比较Kamikawa吻合术与双通道吻合(DTR)术在近端胃切除术后的近期疗效。方法本研究采用倾向性评分匹配研究方法和回顾性队列研究方法。病例纳入标准为年龄20~70岁;经术前内镜活检病理诊断为胃癌;肿瘤直径≤4 cm且位于胃上1/3(包括食... 详细信息

目的比较Kamikawa吻合术与双通道吻合(DTR)术在近端胃切除术后的近期疗效。方法本研究采用倾向性评分匹配研究方法和回顾性队列研究方法。病例纳入标准为年龄20~70岁;经术前内镜活检病理诊断为胃癌;肿瘤直径≤4 cm且位于胃上1/3(包括食管胃交界处);TNM分期为ⅠA、ⅠB或ⅡA期。回顾性收集2020年6月至2023年2月期间在空军军医大学唐都医院胃肠外科行腹腔镜近端胃癌根治术的73例患者临床资料,其中行Kamikawa吻合术19例(Kamikawa组),行DTR术54例(DTR组)。使用R语言进行1∶2倾向性评分匹配患者的一般资料,最终Kamikawa组纳入17例,DTR组纳入34例。比较两组患者的手术相关情况、术后生活质量以及术后并发症等。结果倾向匹配后,两组基线资料差异无统计学意义(均P>0.05)。与DTR组相比较,Kamikawa组手术时间更长[(321.5±15.7)min比(296.8±26.1)min,t=32.056,P2(82~148)ml,z=71.536,P0.05)。中位随访时间(6.1±1.8)个月。在术后生活质量评价中,Kamikawa组较DTR组上消化道造影反流比率较低[0比29.4%(10/34),χ^(2)=6.220,P=0.013],差异有统计学意义;而两组在生活质量评分、随访3个月和6个月胃食管反流病(GERD)量表等方面差异均无统计学意义(均P>0.05)。Kamikawa组的术后并发症发生率为2/17,明显低于DTR组的41.2%(14/34),差异有统计学意义(χ^(2)=4.554,P=0.033)。结论Kamikawa吻合和DTR均为理想的近端胃术后消化道重建方式。虽然Kamikawa吻合耗时略长,对手术团队要求更高,但术后抗反流效果可能更佳。

关键词：胃肿瘤,近端消化道重建 Kamikawa吻合术双通道吻合术