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Selection and characterization of eight freshwater green algae strains for synchronous water purification and lipid production

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Frontiers of Environmental Science & Engineering 2016年第3期10卷 548-558页

作者： Jingjing zhAN qiao zhAng Momei QIN Yu HOng Beijing Key Laboratory for Source Control Technology of Water Pollution College of Environmental Science and Engineering Beijing Forestry University Beijing 100083 China State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology Shenzhen Key Laboratory of Drinking Water Source Safety Control Shenzhen Research Academy of Environmental Sciences Shenzhen 518001 China

The objective of this study is to select and characterize the candidate for synchronous water purification and lipid production from eight freshwater microalgae strains （Chlorella sp. HQ, C. emersonii, C. pyrenoidosa, C. vulgaris, Scenedesmus dimorphus, S. quadricauda, S. obiquus, Scenedesmus sp. LX1）. The strains Chlorella sp. HQ, C. pyrenoidesa, and S. obliquus showed superiority in biomass accumulation, while the top biomass producers did not correspond to the top lipid producers. S. quadricauda achieved higher lipid content （66.1%）, and Chlorella sp. HQ and S. dimorphus ranked down in sequence, with lipid content above 30%. Considering nutrient removal ability （total nitrogen （TN）： 52.97%; total phosphorus （TP）： 84.81%）, the newly isolated microalga Chlorella sp. HQ was the possible candidate for water purification coupled with lipid production. To further investigate the lipid producing and nutrient removal mechanism of candidate microalga, the ultra structural changes especially the lipid droplets under different water qualities （different TN and TP concentrations） were characterized. The results elucidate the nutrient-deficiency （TN： 3.0 mg·L^-1; TP： 0.3 mg·L^-1） condition was in favor of forming lipid bodies in Chlorella sp. HQ at the sub-cellular level, while the biomass production was inhibited due to the decrease in chloroplast number which could further suppress the nutrient removal effect. Finally, a two- phase cultivation process （a nutrient replete phase to produce biomass followed by a nutrient deplete phase to enhance lipid content） was conducted in a photo-bioreactor for Chlorella sp. HQ to serve for algae-based synchronous biodiesel production and wastewater purification.

关键词： freshwater microalgae biomass production lipid accumulation nutrient removal

Double-theodolite measurement systemusedinthei mage calibration of space photographic instrument

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Optoelectronics Letters 2005年第3期 59-62页

作者： LI Yan~(1,2**), qiao Yan-feng~1, SU Wan-xin~1, and LIU ze-xun~11.Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,Chi-na2.Graduate School of Chinese Academy of Sciences, Beijing 100039,China

The purpose of characterizing the image of space photographic instrument is to gain the space included angles from three coordinate axes in the three-dimensional coordinate of the image and the directionality of the three axes of coordinate in the frame of axes of the instrument. The two reference frames will keep in the same direction finally by adjusting according to space angles. This problem was solved by a new high-precision measurement system composed of a double-theodolite and a set of communication system. In the survey system, two TDA5005 total stations from Leica Company will be selected as the double-theodolite and the interdependence of both coordinate systems can be achieved by moving the stations only at one time. Therefore, this measurement system provides a highly efficient and high-precision surveying method to the image calibration of the space photographic instrument. According to the experiment, its measuring accuracy can reach arc-second level.

关键词： Double-theodolite measurement systemusedinthei mage calibration of space photographic instrument

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原发性肺动脉内膜肉瘤一例

海南医学 2024年第19期35卷 2853-2856页

作者：方珍乔树叶以敏柳州市工人医院病理科广西柳州545007

动脉内膜肉瘤是一种罕见恶性间叶肿瘤,其缺乏特征性的临床表现及影像学特点,极易误诊。该肿瘤目前以手术治疗为主,生存期短,预后差。本病例发生于肺动脉,术前超声提示肺动脉栓塞,CT提示肺动脉及其分支充盈缺损或闭塞;术后病理显示肿瘤... 详细信息

动脉内膜肉瘤是一种罕见恶性间叶肿瘤,其缺乏特征性的临床表现及影像学特点,极易误诊。该肿瘤目前以手术治疗为主,生存期短,预后差。本病例发生于肺动脉,术前超声提示肺动脉栓塞,CT提示肺动脉及其分支充盈缺损或闭塞;术后病理显示肿瘤与动脉内壁关系密切,细胞呈束状或不规则排列,细胞梭形或上皮样,异型性明显,可见瘤巨细胞、坏死及病理性核分裂象,免疫组化显示肿瘤细胞主要表达Vimentin、SMA,荧光原位杂交(FISH)检测显示MDM2基因扩增,病理诊断为肺动脉内膜肉瘤。

关键词：肺动脉内膜肉瘤病理学特征免疫组化 MDM2基因

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聚球藻PCC7002 phoR基因的鉴定及其在磷稳态中的功能

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水生生物学报 2024年第8期48卷 1393-1401页

作者：孙巧伟冯苗苗陈思宁陈未中姜海波宁波大学海洋学院宁波315211 南方海洋科学与工程广东省实验室(珠海) 珠海519080

为探究蓝藻低磷适应机制,研究鉴定了海洋聚球藻PCC7002中可能的磷调控基因phoR(A2100),并通过生物信息学的方法对PhoR蛋白理化性质进行了分析,发现phoR基因长度为1296bp,编码431个氨基酸,PhoR蛋白由PAS、HisKA和HATPase_c三部分结构域组... 详细信息

为探究蓝藻低磷适应机制,研究鉴定了海洋聚球藻PCC7002中可能的磷调控基因phoR(A2100),并通过生物信息学的方法对PhoR蛋白理化性质进行了分析,发现phoR基因长度为1296bp,编码431个氨基酸,PhoR蛋白由PAS、HisKA和HATPase_c三部分结构域组成,在不同蓝藻物种中序列较为保守。随后通过原核表达获得了PhoR重组蛋白,并制备其鼠源多克隆抗体,检测了PhoR在聚球藻PCC7002中缺磷诱导时的表达变化,证明PhoR蛋白在磷缺乏时大量诱导表达。为探究PhoR在聚球藻PCC7002中的生理功能,通过缺失突变获得了Mut-phoR突变株,并分析了其生理表型,发现Mut-phoR突变株在磷限制条件下出现显著的生长缺陷及光合作用的损伤,证明PhoR是聚球藻PCC7002适应低磷环境不可缺少的。研究结果为深入认识蓝藻的低磷适应分子机制提供了科学基础。

关键词： phoR 原核表达基因敲除多克隆抗体制备聚球藻PCC7002

Effects of annealing treatment on microstructure and tensile behavior of the Mg-zn-Y-Nd alloy

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Journal of Magnesium and Alloys 2020年第3期8卷 601-613页

作者： L.Y.Sheng B.N.Du z.Y.Hu Y.X.qiao z.P.Xiao B.J.Wang D.K.Xu Y.F.zheng T.F.Xi Shenzhen Institute Peking UniversityShenzhen 518057China Jiangsu University of Science and Technology Zhenjiang 212003China Institute of Metal Research Chinese Academy of SciencesShenyang 110016China dShenzhen Yezhan Electronics Co. LtdShenzhen 518110China

In the present study,the Mg-4zn-0.6Y-0.5Nd alloy was hot extruded and annealed at 200℃,225℃ and 250℃ for different time to optimize microstructure and mechanical *** results exhibit that the dual-size grain structure and linearly distributed secondary phase are the main feature of the as-extruded Mg-zn-Y-Nd alloy,which can be described as the elongated grain is surrounded by the fine equiaxed ***,the as-extruded alloy shows strong{011^(-)0}fiber texture feature,especially for the large elongated *** annealing treatment results in static recrystallization,which increases fine equiaxed grains but decreases large elongated *** addition,the equiaxed grains formed during the annealing treatment demonstrate relative random orientations,which weaken the{011^(-)0}fiber texture of the ***,during the annealing at 225℃and 250℃,the extension twins begin to form in the alloy and weaken the{011^(-)0}fiber texture of the alloy *** annealing treatment has little influence on the linearly distributed secondary phase but promotes the coarsening of small precipitates at 250℃.The annealing treatment could increase the yield and ultimate strength,but the elongation decreases,especially at higher *** a variation can be ascribed to the evolution of texture,grain structure,twinning and precipitation during the annealing treatment.

关键词： Annealing treatment Mg-zn-Y-Nd alloy Microstructure Tensile property

Threshold Multiparty Controlled Teleportation of Arbitrary m-Qubit Quantum Information

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Communications in Theoretical Physics 2009年第10期52卷 593-596页

作者： YAng Yu-Guang WEN qiao-Yan College of Computer Science and Technology Beijing University of Technology Beijing 100124 China State Key Laboratory of Integrated Services Network Xidian University Xi＇an 710071 China State Key Laboratory of Information Security （Graduate University of Chinese Academy of Sciences） Beijing 100049 China State Key Laboratory of Networking and Switching Technology Beijing University of Posts and Telecommunications Beijing 100876 China

We present a （t, .n） threshold multiparty controlled quantum teleportation protocol of an arbitrary m-qubit quantum state between two remote parties. The unknown m-qubit quantum state can be recovered by the receiver under control of a subset of the n controllers if the number of the subset is larger than or equal to a threshold, say, t, but not for any t - 1 or fewer controllers. Our scheme seems to be more practical and more flexible than other existing protocols. The quantum resource required is just m Einstein-Podolsky-Rosen （EPR） pairs plus some single photons. The techniques required are only Bell state measurement, single-qubit unitary operation and yon Neumann measurement. So our scheme is also feasible with present-day technique.

关键词： controlled teleportation threshold controlled teleportation entanglement

女性抑郁症患者应激感受和环状RNA在应激性生活事件与抑郁程度间的链式中介作用

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中华行为医学与脑科学杂志 2024年第4期33卷 317-322页

作者：卜天一乔可欣张计丽王岩邱晓惠乔正学周佳玮黄碧琳陈宇杨艳杰哈尔滨医科大学心理科学与健康管理中心哈尔滨150081

目的:探索应激感受和潜在生物标志物环状RNA hsa_circ_0006862的表达在应激性生活事件和抑郁程度之间的作用路径。方法:2019年1月至2020年8月选取30名女性抑郁症患者和30名健康女性作为研究对象,采集外周血作为环状RNA的来源,进行实时... 详细信息

目的:探索应激感受和潜在生物标志物环状RNA hsa_circ_0006862的表达在应激性生活事件和抑郁程度之间的作用路径。方法:2019年1月至2020年8月选取30名女性抑郁症患者和30名健康女性作为研究对象,采集外周血作为环状RNA的来源,进行实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)以验证hsa_circ_0006862的差异表达,并通过受试者工作特征(receiver operating characteristic,ROC)曲线评估其诊断价值。对30名女性抑郁症患者进行问卷调查,选用自编一般情况调查表、汉密尔顿抑郁量表、社会再适应评定量表、应激感受量表为评估工具。使用SPSS 23.0对数据进行分析,采用Spearman相关分析考察应激性生活事件、应激感受、hsa_circ_0006862表达量与抑郁程度之间的相关性。使用Bootstrap法进行中介效应检验,探究应激感受和hsa_circ_0006862表达量在应激性生活事件和抑郁程度之间的中介作用。结果:qRT-PCR结果表明抑郁组hsa_circ_0006862表达量高于对照组(z=-6.402,P2的曲线下面积为0.762(95%CI=0.637~0.887,P2表达量呈负相关(r=-0.78,P2表达量与应激感受(r=-0.69,P2(95%CI=0.271~0.753)。hsa_circ_0006862表达量在应激性生活事件和抑郁程度之间的中介效应显著,效应量为0.228(95%CI=0.076~0.365)。应激感受和hsa_circ_0006862表达量在应激性生活事件和抑郁程度之间的链式中介效应显著,效应量为0.100(95%CI=0.024~0.213)。结论:应激性生活事件会直接影响女性抑郁症患者的抑郁程度,也可以通过应激感受和hsa_circ_0006862表达间接影响其抑郁程度。

关键词：抑郁症环状RNA 应激性生活事件应激感受中介作用

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基于物理的植物器官实时变形模拟

系统仿真学报 2008年第24期20卷 6587-6591页

作者：陆声链赵春江郭新宇李长峰乔晓军国家农业信息化工程技术研究中心北京100097 上海交通大学数字农业实验室上海200030

近年来,各种方法被用来模拟可变形物体的实时变形。提出了一种基于质点-弹簧系统的植物器官变形模拟方法,并根据交互式设计的要求开发了参数化的植物器官几何造型和弹簧模型生成方法,简化了质点-弹簧模型的构造。实验结果表明,该方法能... 详细信息

近年来,各种方法被用来模拟可变形物体的实时变形。提出了一种基于质点-弹簧系统的植物器官变形模拟方法,并根据交互式设计的要求开发了参数化的植物器官几何造型和弹簧模型生成方法,简化了质点-弹簧模型的构造。实验结果表明,该方法能够获得具有较高真实感的变形效果,能够适用于多种植物器官一般情况下的变形模拟;同时,参数化的几何曲面和物理模型生成能够满足于交互式实时模拟的需要。

关键词：基于物理的模拟植物器官变形实时模拟

Synthesis and Crystal Structure of an Acetate Radical Bridged Dinuclear Copper(Ⅱ) Complex

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Chinese Journal of Structural Chemistry 2007年第3期26卷 347-351页

作者： DU qiao-Yun MA Liang QIN Jian-Hua Department of Chemistry Luoyang Normal University Luoyang 471022 China

A new acetate radical bridged dinuclear copper（ Ⅱ ） complex [（C8H9NO2）2- Cu2（CH3COO）4] has been synthesized by the reaction of Cu（OAc）2 with pyridine-3-ethyl formate and further characterized by X-ray single-crystal diffraction. Crystal data for the title compound： triclinic system, space group Pí, with a = 8.1654（14）, b = 9.7010（16）, c = 9.9005（17） A, α = 83.266（2）, β = 78.887（2）, γ = 72.881（2）°, V= 733.9（2） A^3, Mr = 665.58, z = 1, Dc = 1.506 g/cm^3, F（000） = 342,μ = 1.510 mm^-1, the final R = 0.0392 and wR = 0.1005. According to the structural determination, each unit of the title compound is composed of two Cu（ Ⅱ ） atoms, two pyridine-3-ethyl formate molecules and four acetate ions. Each Cu（Ⅱ） coordinates to oxygen atoms of acetate and nitrogen atoms of pyridine- 3-ethyl formate ligand to give a six-coordinate distorted octahedral geometry. It is worth mentioning that strong Cu-Cu bond can be observed in the title compound.

关键词： pyridine-3-carboxylic acetate radical bridge Cu( Ⅱ ) crystal structure