检索结果-南通市图书馆

Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2022年第2期11卷 238-246页

作者： Yiteng qiao Zhichang Qiu Fengwei Tian Leilei yu Jianxin Zhao Hao Zhang Qixiao Zhai Wei Chen State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiJiangsu 214122China School of Food Science and Technology Jiangnan UniversityWuxiJiangsu 214122China College of Food Science and Engineering Shandong Agricultural UniversityTai’anShandong 271018China National Engineering Research Center for Functional Food Jiangnan UniversityWuxiJiangsu 214122China Wuxi Translational Medicine Research Center Jiangsu Translational MedicineResearch Institute Wuxi BranchWuxiChina

This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal *** *** strains with antibacterial activity(*** CCFM28 and CCFM18)were obtained based on the inhibition-zone *** produced components were identified as bacteriocins through protease treatment,pH adjustment and hydrogen peroxide ***-producing and non-bacteriocin-producing *** strains(*** CCFM28,CCFM18 and NT17-3)caused significant changes in serum immune factors and intestinal flora of normal *** 14 days of intervention,the relative abundance of Firmicutes was significantly decreased,but that of Proteobacteria was significantly increased at the phylum *** the genus level,the administration of three *** strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and ***,there were also different regulations on some probiotic strains,such as Bifidobacterium,Coprococcus and Akkermansia,which were closely related to the antibacterial ability of the bacteriocin and the type of *** results indicated that the intervention of different *** strains could differently change the structure of intestinal flora in normal mice,which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future.

关键词： Pediococcus acidilactici Bacteriocin Immune system Intestinal flora Regulation

在线全文

学校读者我要写书评

暂无评论

多体位医用X射线防护屏的研制

中国医疗设备 2014年第2期29卷 30-33页

作者：余巧生蒋均远仝青英樊水平米彦军张铁骑武警北京市总队第二医院北京100037 武警总医院医学工程科北京100039 北京美邦正悦科技有限公司北京100073

目的结合X线检查诊断的防护需求,研制一种新型多体位X射线防护屏。方法对防护屏的翻转支架、支撑轴、屏蔽布、活动架以及弹簧锁等装置进行技术设计,实现新型多体位X线防护屏的灵活操作。结果该防护屏占用空间小、移动灵活、操作方便、... 详细信息

目的结合X线检查诊断的防护需求,研制一种新型多体位X射线防护屏。方法对防护屏的翻转支架、支撑轴、屏蔽布、活动架以及弹簧锁等装置进行技术设计,实现新型多体位X线防护屏的灵活操作。结果该防护屏占用空间小、移动灵活、操作方便、性能可靠,能有效实现患者X射线投照时的防护。结论研制的新型多体位X射线防护屏可满足X射线受检者多体位的防护要求,避免穿脱传统铅防护衣、帽、裙的麻烦,同时防止附着在铅衣表面细菌的交叉传播,提高了医师操作的便捷性和服务质量。

关键词： X线机 X射线防护屏多体位防护 X射线

在线全文

学校读者我要写书评

暂无评论

黄连素对鸡球虫的防治效果研究

安徽农业科学 2015年第20期43卷 115-116,121页

作者：刘伟伟房志家宋洋乔彧朱倩刘红梅黄志伟东华大学化学化工与生物工程学院上海201620

[目的]探讨黄连素对鸡球虫的防治效果。[方法]将2周龄的雏鸡分为攻虫对照组、空白对照组、加药A组、加药B组,分别给予0、0、300和1 500μg/g体重剂量的黄连素,探讨黄连素的安全性和及其对鸡球虫的治疗效果。[结果]加药A组、加药B组和攻... 详细信息

[目的]探讨黄连素对鸡球虫的防治效果。[方法]将2周龄的雏鸡分为攻虫对照组、空白对照组、加药A组、加药B组,分别给予0、0、300和1 500μg/g体重剂量的黄连素,探讨黄连素的安全性和及其对鸡球虫的治疗效果。[结果]加药A组、加药B组和攻虫对照组雏鸡的相对增重率分别为55.56%、10.71%、26.43%。空白对照组、加药A组、加药B组和攻虫对照组的抗球虫指数依次为200、141.26、59.99、29.29。[结论]黄连素对鸡球虫具有明显的治疗效果,剂量以300μg/g体重为宜。

关键词：鸡球虫病黄连素治疗效果

hVEGFA基因重组腺病毒载体的构建及其转染效率的研究

在线全文

学校读者我要写书评

暂无评论

江苏大学学报（医学版） 2010年第5期20卷 382-385页

作者：李慧勇袁志诚陈波陆培松李巧玉曹侃江苏大学附属人民医院神经外科江苏镇江212002

目的:应用AdEasy复制缺陷型腺病毒载体系统构建携带hVEGFA基因的重组腺病毒载体。方法:采用分子克隆技术,从PCR2.1-hVEGFA质粒中克隆出hVEGFA基因的编码序列,酶切、连接后,插入腺病毒系统中的穿梭载体pAdTrack-CMV,获得重组质粒pAdTrack... 详细信息

目的:应用AdEasy复制缺陷型腺病毒载体系统构建携带hVEGFA基因的重组腺病毒载体。方法:采用分子克隆技术,从PCR2.1-hVEGFA质粒中克隆出hVEGFA基因的编码序列,酶切、连接后,插入腺病毒系统中的穿梭载体pAdTrack-CMV,获得重组质粒pAdTrack-CMV-hVEGFA,将其转化含有腺病毒骨架载体pAdeasy-1的大肠埃希菌BJ5183,进行同源重组,获得重组子pAdeasy-1-hVEGFA。鉴定后,将pAdeasy-1-hVEGFA转染人胚肾细胞(HEK293A细胞),获得含hVEGFA基因的重组腺病毒(rAd-hVEGFA)。反复感染HEK293A细胞扩增腺病毒,50%组织培养感染量法(TCID50)检测病毒滴度,荧光显微镜观测绿色荧光蛋白(GFP)的表达。结果:成功构建出含有hVEGFA基因的腺病毒载体rAd-hVEGFA,转染HEK293A细胞后出现绿色荧光蛋白表达,证明腺病毒载体rAd-hVEGFA转染成功,通过反复感染HEK293A细胞获得大量病毒。结论:成功构建含有hVEGFA基因的腺病毒载体rAd-hVEGFA,可为缺血性脑血管疾病的基因治疗提供基因载体。

关键词： hVEGFA基因腺病毒载体转染缺血性脑血管疾病

A self-assembled framework that interpenetrates in crystal but does not interpenetrate in solution

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2021年第7期64卷 1228-1234页

作者： Bo Yang Jian-Wei Zhang Shang-Bo yu Ze-Kun Wang Pan-Qing Zhang Xiao-Di Yang qiao-Yan Qi Guan-yu Yang Da Ma Zhan-Ting Li College of Chemistry Zhengzhou UniversityZhengzhou 450001China Innovation Research Institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese MedicineShanghai 201203China Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China Department of Chemistry Fudan UniversityShanghai 200438China

A porous supramolecular framework has been for the first time revealed to undergo interpenetration in crystal and noninterpenetration in solution.A new supramolecular organic framework Bu-SOF has been constructed from the co-assembly of a tetracationic tetrahedral monomer and cucurbit[8]uril(CB[8])in water through the encapsulation of two anti-parallel n-butyl chains by CB[8].X-ray diffraction analysis reveals that Bu-SOF forms 3-fold interpenetrated networks in crystals grown by evaporation of its solution in water.1 H NMR,dynamic light scattering and isothermal titration calorimetric experiments confirm that Bu-SOF is also formed in *** samples,prepared by lyophilizing the aqueous solution of Bu-SOF,can adsorb nanoscaled organic dyes,supporting the porosity of the framework and thus non-interpenetration in *** avoidance of interpenetration of Bu-SOF in solution has been attributed to the filling of water inside the porous framework as well as the electrostatic repulsion of the appended bipyridinium units of the tetrahedral building block.

关键词： supramolecular organic framework cucurbituril crystal interpenetration non-interpenetration

选择性黄体期雌激素补充方案对体外受精-胚胎移植治疗结局的影响

在线全文

学校读者我要写书评

暂无评论

生殖医学杂志 2008年第1期17卷 34-37页

作者：张翠莲谢娟珂张少娣乔玉环王姣峰张静郑州大学第一附属医院郑州450052 河南省人民医院生殖医学研究所郑州450003 郑州大学公共卫生学院郑州450052

目的探讨黄体期添加小剂量雌激素的必要性及其对体外受精-胚胎移植(IVF-ET)胚胎种植率和临床妊娠率的影响。方法回顾性分析216个长方案控制性超促排卵(COH)IVF-ET周期的治疗结果,根据其黄体期支持用药情况分为两组:A组:97个周期,单用黄... 详细信息

目的探讨黄体期添加小剂量雌激素的必要性及其对体外受精-胚胎移植(IVF-ET)胚胎种植率和临床妊娠率的影响。方法回顾性分析216个长方案控制性超促排卵(COH)IVF-ET周期的治疗结果,根据其黄体期支持用药情况分为两组:A组:97个周期,单用黄体酮(P)60mg/d及口服地屈孕酮20mg/d;B组:119个周期,对ET日血清雌二醇(E2)水平下降≥30%的89个周期,采用P60mg/d+戊酸雌二醇3mg/d。结果(1)216个治疗周期中,各组的取卵数目、受精率、卵裂率、人绒毛膜促性腺激素(hCG)日E2水平、优质胚胎数、内膜厚度和移植胚胎数目相比均无显著性差异(P>0.05)。(2)A组ET日血清E2下降≥30%时,胚胎种植率和临床妊娠率下降差异有显著性(P<0.05);B组E2下降≥40%时,胚胎种植率和临床妊娠率差异仍有显著性(P<0.05);说明给予补充3mg/d戊酸雌二醇,可改善E2下降30%～40%的患者,而下降≥40%的患者需更大剂量的雌激素补充。(3)两组整体相比,B组胚胎种植率明显高于A组(P<0.05),而妊娠率稍高于A组,但无统计学差异(P=0.15)。结论(1)长方案COH、IVF-EF中,当移植日血清E2水平下降≥30%时,黄体期支持应给予雌激素补充以改善胚胎种植率和临床妊娠率;(2)雌激素补充的剂量要根据E2下降幅度而定,E2下降越大补充剂量也应越大。

关键词：雌激素体外受精-胚胎移植黄体期妊娠率胚胎种植率

在线全文

学校读者我要写书评

暂无评论

不同生育期苜蓿叶蛋白的营养价值

石河子大学学报（自然科学版） 2005年第5期23卷 620-622页

作者：谭增权田丽萍刘青广姜红李玉巧曾凡枝石河子大学生命科学学院新疆石河子832003 石河子大学图书馆新疆石河子832003

利用直接加热法提取了不同生育期的苜蓿叶蛋白,并对其进行了营养价值评价。结果显示,苜蓿叶蛋白较适合少年的需要,其中初花期叶蛋白的营养价值最高。

关键词：苜蓿营养价值生育期

Study on the Rapid Method to Predict Longevity of Controlled Release Fertilizer Coated by Water Soluble Resin

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2008年第9期7卷 1127-1132页

作者： DAI Jian-jun FAN Xiao-lin yu Jian-gang LIU Fang ZHANG qiao College of Resources and Environment Northeast Agricultural University Harbin 150030 P.R.China Fertilizer and Balanced Fertilization Research Lab College of Resources and Environment South China Agricultural University Guangzhou 510642 P.R.China

The study discussed the rapid method to test and predict the longevity of controlled release fertilizers （CRFs） coated by water soluble resin by using the short-term leaching under higher temperature. Pure water dissolving incubation and higher temperature leaching were used to study the patterns of the nutrient release of the CRFs. The correlation analysis between the days at 25℃ and the hours at 80℃ of Trincote 1 and Trincote 2 for the same cumulative release rates were conducted. Patterns of cumulative nutrient release curve followed one factor quadratic regression equation at each given temperatures, and each of relative coefficient was bigger than 0.995. As the temperature increased, nutrients release of the CRFs increased. The longevity of resin coated CRFs were predicted by use of both the cumulative nutrients release equation at 80℃ and the regression equation of release time needed for the same cumulative release rates between 25 and 80℃. There were only 0.3-6.9% relative errors between the tested longevity and predicted one. In conclusion, the longevity of resin coated CRFs could be predicted more quickly and precisely by use of the higher temperature short-term leaching method than that of the traditional differential release rate. The longevity of resin coated CRF could be rapidly and precisely predicted in a few hours by application of the higher temperature shortterm leaching method.

关键词： controlled release fertilizer (CRF) water soluble resin coating longevity of CRF rapid test method

不同处理条件对洋葱鳞茎表皮细胞骨架形态影响的研究

在线全文

学校读者我要写书评

暂无评论

茂名学院学报 2009年第3期19卷 15-19页

作者：马云张妍妍王启钊王伍张巧玉陈改红信阳师范学院生命科学学院河南信阳464000

细胞骨架是活细胞的重要结构,研究细胞骨架的形态对于进一步研究其功能具有重要意义。本研究以洋葱为材料,设置了4个不同的1%Triton X-100抽提非骨架蛋白时间(10min、15min、25min、40min)对洋葱细胞骨架显微观察的关键条件进行了优化,... 详细信息

细胞骨架是活细胞的重要结构,研究细胞骨架的形态对于进一步研究其功能具有重要意义。本研究以洋葱为材料,设置了4个不同的1%Triton X-100抽提非骨架蛋白时间(10min、15min、25min、40min)对洋葱细胞骨架显微观察的关键条件进行了优化,同时分析了细胞环境温度(-18℃、4℃、20℃、50℃),紫外线照射活细胞(0 min6、0 min),0.1mol/L Ca2+处理及0.1%秋水仙素处理对洋葱细胞骨架形态观察效果的影响。结果表明:1%Triton X-100抽提非骨架蛋白25 min观察效果较好;-18℃、50℃,紫外线辐射60 min,0.1mol/L Ca2+和秋水仙素处理均会影响细胞骨架的观察。

关键词：细胞骨架温度紫外线洋葱鳞茎显微观察