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CircRNA DICAR as a novel endogenous regulator for diabetic cardiomyopathy and diabetic pyroptosis of cardiomyocytes

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Signal Transduction and Targeted Therapy 2023年第4期8卷 1889-1900页

作者： Qiong Yuan Yunwei Sun Fan Yang Dan Yan Meihua Shen Zhigang Jin Lin Zhan Guangqi Liu Ling Yang qianyi zhou Zhijun Yu Xiangyu zhou Yang Yu Yong Xu Qingming Wu Jianfang Luo Xiamin Hu Chunxiang Zhang Department of Cardiology the Affiliated Hospital of Southwest Medical University and Key Laboratory of Medical ElectrophysiologyMinistry of EducationInstitute of Cardiovascular Research and Institute of Metabolic DiseasesSouthwest Medical UniversityLuzhou 646000China College of Medicine Wuhan University of Science and TechnologyWuhan 430065China Department of Emergency and Critical Care Medicine Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China China Resource&WISCO General Hospital Wuhan University of Science and TechnologyWuhan 430065China Department of Cardiology Guangdong Cardiovascular InstituteGuangdong Provincial Key Laboratory of Coronary Heart Disease PreventionGuangdong General HospitalGuangzhouChina College of Pharmacy Shanghai University of Medicine and Health SciencesShanghai 210000China

In this study,we identified that a conserved circular RNA(circRNA)DICAR,which was downregulated in diabetic mouse *** had an inhibitory effect on diabetic cardiomyopathy(DCM),as the spontaneous cardiac dysfunction,cardiac cell hypertrophy,and cardiac fibrosis occurred in DICAR deficiency(DICAR+/−)mice,whereas the DCM was alleviated in DICARoverexpressed DICARTg *** the cellular level,we found that overexpression of DICAR inhibited,but knockdown of DICAR enhanced the diabetic cardiomyocyte *** the molecular level,we identified that DICAR-VCP-Med12 degradation could be the underlying molecular mechanism in DICAR-mediated *** synthesized DICAR junction part(DICAR-JP)exhibited a similar effect to the entire *** addition,the expression of DICAR in circulating blood cells and plasma from diabetic patients was lower than that from health controls,which was consistent with the decreased DICAR expression in diabetic *** and the synthesized DICAR-JP may be drug candidates for DCM.

关键词： diabetic endogenous cardiomyopathy

MRTF-A-miR-1247-3p-caspase-2 signaling pathway regulates the apoptosis of cerebral neurons induced by cerebral stroke

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MRTF-A-miR-1247-3p-caspase-2 signaling pathway regulates the...

首届东方药理论坛暨2018年全国心脑血管药理学术会议、上海市药理学会第十九届学术年会

作者： Rong Zhang Weipin zhou Zhijun Yu qianyi zhou Qiong Yuan New Medicine Innovation and Development Institute Department of PharmacyCollege of MedicineWuhan University of Science and Technology Hubei Province Key Laboratory of Occupational Hazard Identification and Control Wuhan University of Science and Technology Department of Biomedical Engineering School of Medicine and School of EngineeringThe University of Alabama at Birmingham

Brain stroke is a leading cause of death ***-related transcription factor-A(MRTF-A) is a co-activator of serum response factor(SRF)-mediated gene expression by bounding with the DNA consensus sequence CC(A/T)6 GG which known as a CArG *** have recently revealed that MRTF-A is involved in the apoptosis of cortical neurons induced by ischemia/reperfusion(I/R).There is a CArG box located in the upstream DNA sequence of miR-1247-3 p by bioinformatics ***,caspase-2 may be a target gene of miR-1247-3 p predicted by ***,whether MRTF-A can regulate the expression of miR-1247-3 p and caspase-2 is a target of miR-1247-3 p remain *** this study,a mouse cerebral I/R injury model,caused by middle cerebral artery occlusion and reperfusion and oxygen-glucose deprivation/reoxygenation(OGD/R) model were *** brain impairment were analyzed by Longa's five score method and TTC *** apoptosis of neurons was measured by TUNEL staining and the level of cleaved caspase-3 protein ***-1247-3 p and pre-miR-1247-3 p mRNA were detected by qPCR and MRTF-A,cleaved caspase-3 and caspase-2 protein level were detected by western *** found that miR-1247-3 p was downregulated during I/R and that LV-miR-1247-3 p overexpression attenuated brain impairment induced by I/*** results were observed in N2 a cells treated with oxygen-glucose deprivation/reoxygenation(OGD/R).Caspase-2 was upregulated in the brain I/R and OGD/R *** miR-1247-3 p mimic inhibited caspase-2 expression and attenuated the apoptosis of N2 a cells induced by OGD/***-miR-1247-3 p overexpression downregulated the caspase-2 protein expression and reduced apoptosis of I/R ***-A overexpression upregulated pre-miR-1247-3 p and mature miR-1247-3 p levels and attenuated apoptosis induced by OGD/***,it's anti-apoptosis function could be blocked by miR-1247-3 p *** results suggest that the MRTF-A-miR-1247-3 p-caspase-2 s

关键词： miR-1247-3p caspase-2 apoptosis myocardin-related transcription factor-A ischemia/reperfusion

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干旱胁迫下大蒜叶绿素合成基因家族鉴定分析

植物资源与环境学报 2022年第3期31卷 54-64,74页

作者：王紫彤周倩怡田洁青海大学农林科学院青海省蔬菜遗传与生理重点实验室青海西宁810016 青海大学省部共建三江源生态与高原农牧业国家重点实验室青海西宁810016

为探究干旱胁迫对大蒜(Allium sativum Linn.)叶绿素合成的影响,本研究基于大蒜转录组测序数据,筛选出响应干旱胁迫的大蒜叶绿素合成基因家族成员,利用实时荧光定量PCR(qRT-PCR)技术验证其表达情况后,采用生物信息学方法鉴定其序列属性... 详细信息

为探究干旱胁迫对大蒜(Allium sativum Linn.)叶绿素合成的影响,本研究基于大蒜转录组测序数据,筛选出响应干旱胁迫的大蒜叶绿素合成基因家族成员,利用实时荧光定量PCR(qRT-PCR)技术验证其表达情况后,采用生物信息学方法鉴定其序列属性。结果表明:从大蒜转录组数据中搜索得到的17个叶绿素合成基因家族中,筛选出6个积极响应干旱胁迫的大蒜叶绿素合成基因家族,即AsHEMA、AsHEMB、AsGSA、AsCHLD、AsCHLI和AsCAO。qRT-PCR和RNA-Seq结果显示:干旱胁迫6 d,AsHEMA、AsHEMB、AsGSA、AsCHLD、AsCHLI和AsCAO基因家族成员的表达量总体上显著增加。生物信息学分析结果显示:大蒜上述6个基因家族32个unigenes编码序列的氨基酸残基数为104~773,理论相对分子质量为11978~84411,理论等电点为pI 4.78至pI 9.86,不稳定指数为24.66~60.13,脂溶系数为80.04~104.59;除AsGSA家族外,其余5个家族成员的总平均亲水性为负值,即AsGSA家族成员为亲水蛋白,其余5个家族成员为疏水蛋白。保守基序分析结果表明:大蒜这6个叶绿素合成基因家族成员编码的氨基酸序列具有较高的保守性和相似性,其中,AsHEMA、AsGSA和AsCHLD家族高度保守;AsHEMB、AsCHLI和AsCAO家族保守性也较高,仅个别家族成员存在差异。进化树结果显示:大蒜同一叶绿素合成基因家族成员聚在一起,说明各基因家族的成员在进化上具有较高的保守性和相似性;AsHEMA、AsHEMB、AsCHLD、AsCHLI和AsCAO基因家族与单子叶植物小麦(Triticum aestivum Linn.)、石刁柏(Asparagus officinalis Linn.)和玉簪〔Hosta plantaginea(Lam.)Aschers.〕相关基因的同源性较高。上述研究结果显示:AsHEMA、AsHEMB、AsGSA、AsCHLD、AsCHLI和AsCAO基因家族的保守性和相似性较高,在大蒜响应干旱胁迫中发挥重要作用。

关键词：大蒜干旱胁迫叶绿素合成基因生物信息学分析

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基于互联网大数据的脱敏分析技术研究

数据分析与知识发现 2018年第2期2卷 58-63页

作者：周倩伊王亚民王闯西安电子科技大学经济与管理学院西安710126

【目的】基于现有的脱敏技术,改进匿名组的划分效果,得到较优的脱敏模型及算法。【方法】基于k-匿名技术,改进维度划分标准,以KD树作为存储结构,构造新算法。利用Python实现程序,比较所产生的匿名组数量、NCP百分比,验证算法的可行性与... 详细信息

【目的】基于现有的脱敏技术,改进匿名组的划分效果,得到较优的脱敏模型及算法。【方法】基于k-匿名技术,改进维度划分标准,以KD树作为存储结构,构造新算法。利用Python实现程序,比较所产生的匿名组数量、NCP百分比,验证算法的可行性与有效性。【结果】新算法能够使得脱敏后整个数据集所生成的匿名组个数达到最大。且NCP百分比低于同类算法。【局限】对于有某一属性离散程度显著的数据集,循环计算划分维度较为繁琐。【结论】新算法相比于传统算法增加了匿名组个数,相比于同类算法,信息损失较低。

关键词：数据脱敏 k-匿名模型取整划分

非牧区以发热为首发症状18例布鲁菌病临床特征分析

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中华传染病杂志 2014年第4期32卷 229-232页

作者：周倩宜杨文杰天津市第一中心医院感染科 300192

目的回顾性分析非牧区以发热为首发症状布鲁菌病的临床特征,探讨早期诊断方法.方法天津市第一中心医院感染科2010年1月至2013年6月收治的布鲁菌病住院患者18例,分析患者临床症状、血常规、血培养、布鲁菌虎红玻片凝集试验等指标,结合... 详细信息

目的回顾性分析非牧区以发热为首发症状布鲁菌病的临床特征,探讨早期诊断方法.方法天津市第一中心医院感染科2010年1月至2013年6月收治的布鲁菌病住院患者18例,分析患者临床症状、血常规、血培养、布鲁菌虎红玻片凝集试验等指标,结合治疗和随访,分析其临床特征.结果以发热为首发症状布鲁菌病患者中年男性有烟酒史的多见,12例无基础疾病.12例无明确接触史,4例有牛羊接触史,1例进食过未煮熟牛羊肉,1例饲养犬.18例患者均有发热,其中高热15例,不规则热13例.血常规白细胞计数正常12例,白细胞计数减少6例;杆状细胞比例升高,细胞核左移6例;血小板计数减少3例;ALT、AST增高13例;C反应蛋白升高14例;血液或骨髓细菌培养为马耳他布鲁菌12例;布鲁菌虎红玻片凝集试验阳性18例.中性粒细胞比例和淋巴细胞比例均正常.治疗药物为莫西沙星、左氧氟沙星、异帕米星、依替米星、阿米卡星、利福平、米诺环素,选两种或三种药物联合治疗,随访4～11个月,预后好.结论非牧区以发热待查为首诊的患者要警惕布鲁菌病可能,接诊医师应对本病保持警惕性,详细问诊接触史,行实验室检查,明确诊断并给予及时治疗可避免严重并发症的发生.

关键词：布鲁菌病非牧区发热诊断

大蒜2个中性/碱性转化酶基因AsNI的克隆与表达分析

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华北农学报 2023年第4期38卷 54-64页

作者：周倩怡黄思杰田洁青海大学农林科学院青海省高原种质资源研究与利用实验室青海西宁810016 生态环境部南京环境科学研究所江苏南京210042

中性/碱性转化酶作为植物蔗糖代谢的关键酶,主要参与植物生长发育、逆境胁迫响应等过程。为探究大蒜AsNI对逆境胁迫的响应模式,以乐都紫皮大蒜为试验材料,克隆得到2个中性/碱性转化酶基因,并进行了生物信息学和表达特性分析。结果表明,A... 详细信息

中性/碱性转化酶作为植物蔗糖代谢的关键酶,主要参与植物生长发育、逆境胁迫响应等过程。为探究大蒜AsNI对逆境胁迫的响应模式,以乐都紫皮大蒜为试验材料,克隆得到2个中性/碱性转化酶基因,并进行了生物信息学和表达特性分析。结果表明,AsNI1和AsNI2的开放阅读框分别为522,1203 bp,编码173,400个氨基酸;AsNI1与AsNI2均为亲水性蛋白,预测定位于细胞质,具有Glyco_hydro_100结构域;但二者的氨基酸序列相似性仅为25.75%,AsNI2含有1个糖基化位点,而AsNI1未检测到糖基化位点,二者的亲缘关系较远。蛋白互作网络分析结果表明,AsNI2与AsNI1可能参与不同的生化过程。启动子序列分析发现,AsNI1和AsNI2的启动子区域含有多个与响应相关的顺式作用元件,其中AsNI2启动子的干旱和低温胁迫响应元件个数显著大于AsNI1。通过对启动子转录因子结合位点进行预测发现,二者含有不同种类及数量的结合位点,表明AsNI1和AsNI2可行使不同的基因功能。qRT-PCR检测发现,AsNI的表达具有明显的组织特异性,AsNI1和AsNI2分别在根和鳞茎中表达量最高。同时,逆境胁迫能够诱导AsNI表达,低温和干旱处理下均以AsNI2的响应程度强于AsNI1。其中,低温胁迫以诱导叶片AsNI2的表达为主,干旱胁迫以诱导根系AsNI2的表达为主。通过分析AsNI的序列特征和表达模式,验证了AsNI的抗逆功能。

关键词：大蒜中性/碱性转化酶基因基因克隆表达分析逆境胁迫