检索结果-南通市图书馆

Efficient reduction ofβ-lactoglobulin allergenicity in milk using Clostridium tyrobutyricum Z816

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2023年第3期12卷 809-816页

作者： qianru zhao Yuwei Wang Zhengming Zhu Quanyu zhao Liying Zhu Ling Jiang College of Biotechnology and Pharmaceutical Engineering State Key Laboratory of Materials-Oriented Chemical EngineeringNanjing Tech UniversityNanjing 210009China College of Food Science and Light Industry Nanjing Tech UniversityNanjing 210009China School of Pharmaceutical Sciences Nanjing Tech UniversityNanjing 211816China School of Chemistry and Molecular Engineering Nanjing Tech UniversityNanjing 210009China

Milk allergy is one of the most common food allergies,affecting 6%of young children,andβ-lactoglobulin(β-LG)is the main milk allergen.Clostridium tyrobutyricum Z816 was selected for the degradation ofβ-LG,which was successfully reduced by about 90%using permeabilized bacteria under the optimized conditions.The hydrolyzed peptides were identified by liquid chromatography-tandem mass spectrometry(LC-MS/MS)and analyzed by molecular modeling,which indicated that C.tyrobutyricum Z816 could effectively degrade the antigenic epitopes ofβ-LG.Finally,the concentration and digestibility ofβ-LG in actual samples was quantified using enzyme-linked immunosorbent assay(ELISA)and gastrointestinal digestion simulation experiments.The results showed more than 92%ofβ-LG in actual samples was hydrolyzed,and the gastric and total digestibility of whey protein isolate(WPI)was improved by 85.96%and 64.51%,respectively.Therefore,C.tyrobutyricum Z816 offers an effective method to degradeβ-LG and reduce the occurrence of milk allergies,which has great significance for the development of hypoallergenic dairy products.

关键词： Milk allergy β-Lactoglobulin Clostridium tyrobutyricum Permeabilized bacteria

一种基于DX3型道口信号系统的平交道口防护方法

在线全文

学校读者我要写书评

暂无评论

铁路通信信号工程技术 2024年第5期21卷 42-45,69页

作者：赵茜茹中铁大桥勘测设计院集团有限公司武汉430074

基于DX3型道口信号系统提出一种新的道口防护方法,即在列车信号开放条件中加入道口看守员确认道口防护完成以及遮断信号机状态的条件,通过道口防护完全的状态来卡控列车信号机的开放条件。当所有条件均满足时,可以实现不需要延时开放列... 详细信息

基于DX3型道口信号系统提出一种新的道口防护方法,即在列车信号开放条件中加入道口看守员确认道口防护完成以及遮断信号机状态的条件,通过道口防护完全的状态来卡控列车信号机的开放条件。当所有条件均满足时,可以实现不需要延时开放列车信号就能够实现保证平交道口行车安全的目的。同时,大大减轻交通拥堵压力,提高铁路列车和道路车辆的运输效率,提升铁路平交道口的管理水平。本方法适用于不同牵引联锁或闭塞制式的单、双、多线的站内或区间道口。

关键词：平交道口 DX3型道口信号系统延时开放列车信号

维普期刊数据库博看期刊

高效液相色谱法在酱油食品添加剂含量测定中的应用

在线全文

学校读者我要写书评

暂无评论

食品安全导刊 2024年第7期 103-106页

作者：赵倩薷微山县检验检测中心山东济宁277600

为验证酱油中食品添加剂是否符合国家标准限量要求,本文建立了高效液相色谱法测定市售酱油中安赛蜜、苯甲酸、柠檬黄、山梨酸、糖精钠、苋菜红、咖啡因和日落黄等8种食品添加剂的含量。结果表明,8种食品添加剂在各自的线性范围内线性关... 详细信息

为验证酱油中食品添加剂是否符合国家标准限量要求,本文建立了高效液相色谱法测定市售酱油中安赛蜜、苯甲酸、柠檬黄、山梨酸、糖精钠、苋菜红、咖啡因和日落黄等8种食品添加剂的含量。结果表明,8种食品添加剂在各自的线性范围内线性关系良好,相关系数均大于等于0.999 0;平均加标回收率在93.58%~98.27%,RSD <2%;5种酱油中8种食品添加剂含量均符合国家限量要求。本方法操作简单、反应速度较快,且准确性较高,可用于食品添加剂快速筛查。

关键词：高效液相色谱法酱油食品添加剂含量测定

Scheduling Problems of Automated Guided Vehicles in Automated Container Terminals Using a Genetic Algorithm

在线全文

学校读者我要写书评

暂无评论

Scheduling Problems of Automated Guided Vehicles in Automate...

Stacking Position Allocation Model Based on Automatic Rail Crane

作者： Yufan Gan Shouwen Ji qianru zhao Dong Guo Beijing Jiaotong University Beijing Jiaotong University Haibin College Beijin University of City Engineering and Architecture

The automated guided vehicle（AGV） is adopted as horizontal transport equipment in automated container terminals.In order to improve the operation efficiency of the AGV,we constructed a mixed integer programming model for AGV scheduling optimization in unloading operations in an automated container terminal with a vertical layout.The model aimed at minimizing the end time of the last task and the time of AGV invalid operation.The constraints included the operation limit and the time window.We also designed a genetic algorithm to solve the model,and set three experiments to prove the superiority of the method.The experimental results show that the genetic algorithm can significantly improve the calculation efficiency in large-scale AGV scheduling problem,and the scheduling model we constructed can better improve the operation efficiency of the AGV.We also simply studied the problem of the optimal number of AGV configurations.

关键词： Automated guide vehicle （AGV） Scheduling Genetic algorithm Automated container terminals

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Stacking Position Allocation Model Based on Automatic Rail C...

The Turbulent Schmidt Number for Transient Contaminant Dispersion in a Large Ventilated Room Using a Realizable k-εModel

作者： Jia Rong Shouwen Ji qianru zhao Dong Guo Beijing Jiaotong University Beijing Jiaotong University Haibin College Beijin University of City Engineering and Architecture

In order to reduce the energy consumption of the automated container terminal,based on the operation of the container in the yard,this paper used the travel distance of the automatic rail crane to represent the energy consumption problem to study the problem of container allocation.Under the premise that the operating time of inbound container was known,this paper took the number of pressurized containers as a limiting condition,and established the allocation model of the storage space to minimize the travel distance of the automatic rail crane.Considering that the model had a large calculation scale and was an NPhard problem,a genetic algorithm was used to solve it.Example analysis results show that the model and algorithm can effectively reduce the travel distance of the automatic rail crane by 17.02% under the premise of controlling the number of pressure boxes.

关键词： Automated track crane Automated terminal Container allocation Genetic algorithm Terminal energy consumption

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Fluid Dynamics & Materials Processing 2024年第4期20卷 829-846页

作者： Fei Wang Qinpeng Meng Jinchi zhao Xin Wang Yuhong Liu qianru Zhang School of Environment and Architecture University of Shanghai for Science and TechnologyShanghai200093China

Buildings with large open spaces in which chemicals are handled are often exposed to the risk of explosions.Computational fluid dynamics is a useful and convenient way to investigate contaminant dispersion in such large spaces.The turbulent Schmidt number(Sc_(t))concept has typically been used in this regard,and most studies have adopted a default value.We studied the concentration distribution for sulfur hexafluoride(SF_(6))assuming different emission rates and considering the effect of Sc_(t).Then we examined the same problem for a light gas by assuming hydrogen gas(H_(2))as the contaminant.When SF_(6) was considered as the contaminant gas,a variation in the emission rate completely changed the concentration distribution.When the emission rate was low,the gravitational effect did not take place.For both low and high emission rates,an increase in S_(ct) accelerated the transport rate of SF_(6).In contrast,for H_(2) as the contaminant gas,a larger S_(ct) could induce a decrease in the H_(2) transport rate.

关键词： Large space CFD turbulent Schmidt number contaminant dispersion emission rate

超高效液相色谱-串联质谱法同时测定化妆品中83种糖皮质激素

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

色谱 2023年第12期41卷 1084-1094页

作者：赵倩茹刘骅孟雅萍李翔高瑞芳李祥胜安徽省食品药品检验研究院安徽合肥230051

近年来化妆品中非法添加糖皮质激素的恶性事件常有发生,因此建立一个高通量且简便高效的检测方法,对保障消费者的用妆安全具有现实意义。研究建立了超高效液相色谱-串联质谱法同时测定化妆品中83种糖皮质激素的分析方法。以化妆品中常... 详细信息

近年来化妆品中非法添加糖皮质激素的恶性事件常有发生,因此建立一个高通量且简便高效的检测方法,对保障消费者的用妆安全具有现实意义。研究建立了超高效液相色谱-串联质谱法同时测定化妆品中83种糖皮质激素的分析方法。以化妆品中常用的水、乳液、膏霜(o/w型)3种基质为研究对象,对样品前处理和色谱-质谱条件进行优化,最终确定样品经乙腈涡旋分散、超声提取、过滤后,采用Thermo Accucore PFP色谱柱(100 mm×2.1 mm,2.6μm)分离,以0.1%(v/v)乙酸乙腈和0.1%(v/v)乙酸水溶液为流动相梯度洗脱,在电喷雾正离子模式(ESI+)下以动态多反应监测(MRM)方式测定,外标法定量。结果表明,83种糖皮质激素在2~200μg/L范围内线性关系良好,相关系数(r)均大于0.995,在3个不同添加水平下,平均回收率为74.5%~112.4%,相对标准偏差(RSD,n=6)为0.8%~9.9%,方法的检出限(LOD,S/N≥3)和定量限(LOQ,S/N≥10)分别为0.001~0.023μg/g和0.002~0.076μg/g。采用该方法对市售的41批化妆品进行83种糖皮质激素筛查,共发现4批阳性样品,分别检出氟轻松、倍氯米松双丙酸酯、醋酸地索奈德及地索奈德,含量范围为0.53~634.27μg/g,其中醋酸地索奈德尚未列入法定检测标准的筛查范畴。该方法简便快捷,灵敏度高,重复性好,是对现行检测标准的补充,适用于多种基质类型化妆品中83种糖皮质激素的快速定性定量筛查,为化妆品质量的日常监督提供可靠的技术支持。

关键词：超高效液相色谱-串联质谱糖皮质激素化妆品非法添加

龙血素B碳桥结构修饰对其选择性调节离子通道蛋白功能的影响

在线全文

学校读者我要写书评

暂无评论

中南民族大学学报（自然科学版） 2022年第5期41卷 527-535页

作者：尹世金张旭赵倩茹骆滨王强戴康中南民族大学药学院武汉430074

为了对龙血素B碳桥结构进行改造,得到对钾、钠离子通道蛋白功能调节选择性更强的衍生物,以2,4,6-三甲氧基-4′-苄氧基查尔酮和2,4,6-三甲氧基苄胺为原料合成了OB(碳桥具有α,β-不饱和双键的龙血素B衍生物)和AB(碳桥具有酰胺键的龙血素... 详细信息

为了对龙血素B碳桥结构进行改造,得到对钾、钠离子通道蛋白功能调节选择性更强的衍生物,以2,4,6-三甲氧基-4′-苄氧基查尔酮和2,4,6-三甲氧基苄胺为原料合成了OB(碳桥具有α,β-不饱和双键的龙血素B衍生物)和AB(碳桥具有酰胺键的龙血素B衍生物),通过核磁共振与质谱技术对合成的化合物进行了结构表征,并在HEK-293T细胞和急性分离的小鼠背根神经节细胞(dorsal root ganglion,DRG)当中进行了全细胞膜片钳实验,观察改造的龙血素衍生物对钾通道和钠通道的作用.OB对Kv1.3通道的抑制活性与龙血素B相当,但对DRG细胞河豚毒素敏感型钠通道及Nav1.7通道的活性有所降低.AB对DRG钠通道和Nav1.7通道的作用相比龙血素B略有减弱,但几乎丧失了对Kv1.3通道的抑制活性.以上结果表明,对龙血素B的碳桥进行结构修饰,能够改善其相应产物的理化性质,提高部分产物对离子通道蛋白的选择性.

关键词：龙血素B 结构修饰选择性钾通道钠通道

心肌线粒体能量代谢在心血管疾病中的研究进展

在线全文

学校读者我要写书评

暂无评论

中国心血管杂志 2022年第1期27卷 90-93页

作者：孙侠赵倩茹袁伟江苏大学附属医院心内科镇江212000

目前心血管疾病已成为全球人类死亡的主要原因之一,线粒体作为三大营养物质经三羧酸循环产生ATP的主要场所,在心血管疾病的发生、进展过程中起着巨大影响作用。本文从心肌线粒体的能量代谢功能、相关调控途径、与心血管疾病的关系以及... 详细信息

目前心血管疾病已成为全球人类死亡的主要原因之一,线粒体作为三大营养物质经三羧酸循环产生ATP的主要场所,在心血管疾病的发生、进展过程中起着巨大影响作用。本文从心肌线粒体的能量代谢功能、相关调控途径、与心血管疾病的关系以及治疗药物方面入手,在心肌线粒体代谢方面为治疗心血管疾病提供思路与方向。

关键词：线粒体能量代谢活性氧心血管疾病