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Longitudinal proteomic investigation of cOVID-19 vaccination

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Protein & Cell 2023年第9期14卷 668-682页

作者： Yingrui Wang qianru zhu Rui Sun Xiao Yi Lingling Huang Yifan Hu Weigang Ge Huanhuan Gao Xinfu Ye Yu Song Li Shao Yantao Li Jie Li Tiannan Guo Junping Shi Marker Lab Westlake Laboratory of Life Sciences and BiomedicineKey Laboratory of Structural Biology of Zhejiang ProvinceSchool of Life SciencesWestlake University18 Shilongshan RoadHangzhou 310024China Institute of Basic Medical Sciences Westlake Institute for Advanced Study18 Shilongshan RoadHangzhou 310024China Research Centerfor Industries of theFuture WestlakeUniversity600D unyu RoadHangzhou 310030China Center for Infectious Disease Research Westlake University18 Shilongshan RoadHangzhou 310024China Westlake Omics(Hangzhou)Biotechnology Co. Ltd.Hangzhou 310024China Department of Translational Medicine Platform The Affiliated Hospital of Hangzhou Normal UniversityHangzhou 310015China The Fourth School of Clinical Medicine Zhejiang Chinese Medical UniversityHangzhou 310053China Medical college of Hangzhou Normal University Hangzhou 311121China Department of Infectious Diseases Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China Institute of Viruses and Infectious Diseases Nanjing UniversityNanjing 210093China Department of Infectious and Hepatology Diseases The Affiliated Hospital of Hangzhou Normal UniversityHangzhou 310015China

Although the development of covID-19 vaccines has been a remarkable success,the heterogeneous individual antibody generation and decline over time are unknown and still hard to *** this study,blood samples were collected from 163 participants who next received two doses of an inactivated COvVID-19 vaccine(CoronaVac)at a 28-day *** TMT-based proteomics,we identified 1,715 serum and 7,342 peripheral blood mononuclear cells(PBMCs)*** proposed two sets of potential biomarkers(seven from serum,five from PBMCs)at baseline using machine learning,and predicted the individual seropositivity 57 days after vaccination(AUC=0.87).Based on the four PBMC's potential biomarkers,we predicted the antibody persistence until 180 days after vaccination(AUC=0.79).Our data highlighted characteristic hematological host responses,including altered lymphocyte migration regulation,neutrophil degranulation,and humoral immune *** study proposed potential blood-derived protein biomarkers before vaccination for predicting heterogeneous antibody generation and decline after coVID-19 vaccination,shedding light on immunization mechanisms and individual booster shot planning.

关键词： COVID-19 vaccination proteomics neutralizing antibodies(NAbs) machine learning

The Effect of siRNA-VEGF on the Growth of REC in Retina Pigment Epithelial Cell and Retinal Endothelial Cell Co-culture System

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Eye Science 2011年第2期26卷 75-79页

作者： Jin Ma Zhaomeng Shen qianru Zhang Tiepei zhu Ke Yao Eye Center the Second Affiliated Hospital School of Medicine of Zhejiang UniversityHangzhou 310009 China

Purpose:To investigate the effect of small interfering RNA (siRNA)targeting VEGF of retinal pigment epithelium (RPE) cells on the growth activity of human retinal vascular endothelial cells (RECs) under a co-culture ***:By applying the vector.(pGPU6)-based siRNA plasmid gene silence system,we specifically silenced VEGF expression of RPE cells (ARPE-19) through plasmid (pG-PU6-VEGFA-siRNA)*** transcription polymerase chain reaction(RT-PCR) was applied for selecting the most efficient siRNA segment among three pGPU6-VEGF-siRNA groups (siRNA-1,siRNA-2 and siRNA-3).Treated RPE cells were co-cultured with RECs in a co-culture system made up of a 24-well culture plate and transwell inserts assembled inside During 7-day culture period,the growth ca-pacity of RECs were observed and tested in the form of cell counting *** groups were established in this study:RPE cells transfected with pGPU6-VEGF-siRNA and co-cultured with RECs (group A),RPE cells transfected with siR-NA null vector and co-cultured with RECs (group B),and RECs cultured alone (group C).Results:After transfection,VEGF expression levels of RPE cells in three pGPU6-VEGF-siRNA groups (siRNA-1,siR-NA-2 and siRNA-3)evaluated by RT-PCR were 2.56 ±0.45,1.17 ±0.38 and 4.39 ±0.51,respectively (n =10).siRNA-2 was selected as the foremost segment for transfection (P 0.05,dunnett's test).RECs in group A proliferated muchfaster during the first four days ***:Delivery of siRNA targeting VEGF plays an efficient role in down-regulating VEGF expression in RPE cells,therefore modulating the growth activity of RECs under a co-culture system in *** application of this techniqu

关键词：血管内皮生长因子血管内皮细胞 siRNA 共培养体系人视网膜上皮细胞色素经济共同体

Beta-elemene inhibits the growth of KDM6A-null bladder cancer cells by suppressing the epithelial-mesenchymal transition

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Clinical Traditional Medicine and Pharmacology 2024年第2期5卷 15-24页

作者： Ruonan Zhang Jiao Feng Yintao Zheng qianru zhu Bo Xiang Qibiao Wu Xinbing Sui The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health Cancer Research Institute and School of Basic Medical SciencesCentral South UniversityChangsha 410008HunanChina School of Pharmacy Hangzhou Normal UniversityHangzhou 311121ZhejiangChina Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines Engineering Laboratory of Development and Application of Traditional Chinese MedicinesCollaborative Innovation Center of Traditional Chinese Medicines of Zhejiang ProvinceHangzhou Normal UniversityHangzhou 311121ZhejiangChina Department of Gastrointestinal and Pancreatic Surgery Key Laboratory of Gastroenterology of Zhejiang ProvinceZhejiang Provincial People’s HospitalHangzhou 310014ZhejiangChina School of Pharmaceutical Sciences Zhejiang Chinese Medical UniversityHangzhou 311402ZhejiangChina State Key Laboratory of Quality Research in Chinese Medicines Faculty of Chinese MedicineMacao University of Science and TechnologyMacao 999078China

Background:Bladder cancer is a highly prevalent and lethal malignant tumor characterized by frequent mutations/deletions of lysine-specific demethylase 6A(KDM6A),which is suggested to be a key event in cancer progression and ***-elemene has been shown to inhibit metastasis and growth of various tumors,but its effect on KDM6A-null bladder cancer cells remains ***:This study aimed to investigate the potential and molecular mechanism ofβ-elemene in inhibiting the growth of KDM6A-null bladder ***:This study examined the migration ability and viability of RT-4(KDM6A wild-type)and KU19-19(KDM6A-null)cell lines using wound healing assay and CCK-8,*** inhibitory effect ofβ-elemene on KU19-19 cell migration was evaluated using transwell and immunofluorescence assays,and the expression of transfer-related proteins and genes was analyzed through western blot and qRT-PCR,*** docking was performed to predict the targeting ofβ-elemene,and the effects were confirmed in KDM6Aknockdown RT-4 ***,the therapeutic effect ofβ-elemene on bladder cancer was tested in animal ***:The study observed that loss of KDM6A increased bladder cancer cell migration,with KU19-19 exhibiting significantly stronger migration than *** investigation revealed thatβ-elemene effectively inhibited KU19-19 cell migration,likely through targeting EZH2 as determined by molecular *** of KDM6A inhibited KU19-19 metastasis,while knockdown of KDM6A in RT-4 cells enhanced cell migration,which was reversed byβ-elemene ***,in vivo testing revealed a significant suppression of KU19-19 cell growth withβ-elemene administered at a dosage of 100 mg/***:β-elemene has the potential to suppress the growth of KDM6A-null bladder cancer by inhibiting epithelial-mesenchymal transition(EMT),which could make it a promising therapeutic option for patients with KDM6A-null bladder cancer.

关键词： β-Elemene Lysine-specific demethylase 6A(KDM6A) Bladder cancer Metastasis Epithelial-mesenchymal transition(EMT)

Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis

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Bioactive Materials 2022年第7期7卷 23-36页

作者： Ruonan Zhang Ting Pan Yu Xiang Mingming Zhang Han Xie Zimao Liang Bi Chen Cong Xu Jing Wang Xingxing Huang qianru zhu Ziming Zhao Quan Gao Chengyong Wen Wencheng Liu Weirui Ma Jiao Feng Xueni Sun Ting Duan Elaine Lai-Han Leung Tian Xie Qibiao Wu Xinbing Sui State Key Laboratory of Quality Research in Chinese Medicines Faculty of Chinese MedicineMacao University of Science and TechnologyMacao School of Pharmacy and Department of Medical Oncology The Affiliated Hospital of Hangzhou Normal UniversitySchool of MedicineHangzhou Normal UniversityHangzhou311121ZhejiangChina Key Laboratory of Elemene Class Anti-Cancer Chinese Medicines China Engineering Laboratory of Development and Application of Traditional Chinese Medicines China Collaborative Innovation Center of Traditional Chinese Medicines of Zhejiang Province Hangzhou Normal UniversityHangzhou311121ZhejiangChina Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health Guangzhou510006China

Curcumenol,an effective ingredient of Wenyujin,has been reported that exerted its antitumor potential in a few cancer ***,the effect and molecular mechanism of curcumenol in lung cancer are largely ***,we found that curcumenol induced cell death and suppressed cell proliferation in lung cancer ***,we demonstrated that ferroptosis was the predominant method that contributed to curcumenol-induced cell death of lung cancer in vitro and vivo for the first ***,using RNA sequencing,we found that the long non-coding RNA H19(lncRNA H19)was significantly downregulated in lung cancer cells treated with curcumenol,when compared to untreated *** of lncRNA H19 eliminated the anticancer effect of curcumenol,while lncRNA H19 knockdown promoted ferroptosis induced by curcumenol ***,we showed that lncRNA H19 functioned as a competing endogenous RNA to bind to miR-19b-3p,thereby enhanced the transcription activity of its endogenous target,ferritin heavy chain 1(FTH1),a marker of *** conclusion,our data show that the natural product curcumenol exerted its antitumor effects on lung cancer by triggering ferroptosis,and the lncRNA H19/miR-19b-3p/FTH1 axis plays an essential role in curcumenol-induced ferroptotic cell ***,our findings will hopefully provide a valuable drug for treating lung cancer patients.

关键词： Curcumenol Ferroptosis Lung cancer lncRNA H19 miRNA-19b-3p FTH1

新文科背景下英语专业翻译课程多模态教学实践研究

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创新创业理论研究与实践 2022年第23期5卷 137-139页

作者：朱倩茹成都文理学院四川成都610400

新文科建设是国家教育战略之一,充分体现了教育改革的迫切性。在此背景下,各科教学活动都要与时俱进,顺应时代要求。该文从教学目标、课程设置和评价体系等方面对新文科背景下英语专业翻译课程面临的问题进行了分析,并结合英语专业翻译... 详细信息

新文科建设是国家教育战略之一,充分体现了教育改革的迫切性。在此背景下,各科教学活动都要与时俱进,顺应时代要求。该文从教学目标、课程设置和评价体系等方面对新文科背景下英语专业翻译课程面临的问题进行了分析,并结合英语专业翻译课程教学实践,采用多模态教学模式,从育人目标、师资团队、课程资源、教学方式和教学评价等方面探索教学改革实践,以提高翻译课程教学质量,并为类似教学改革提供借鉴。

关键词：新文科英语专业翻译教学多模态教学

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全程护理对心脏手术患者焦虑抑郁情绪的影响

国际精神病学杂志 2019年第4期46卷 737-739,747页

作者：朱倩如周芮伊郑州大学第一附属医院

目的探讨全程护理模式对心脏手术患者焦虑、抑郁情绪的改善效果。方法研究资料均为本院于2016年4月~2018年4月期间收治的行心脏手术治疗患者,共106例,随机分组,对照组和观察组分别开展常规护理服务、全程护理服务,各53例,评价两组焦虑... 详细信息

目的探讨全程护理模式对心脏手术患者焦虑、抑郁情绪的改善效果。方法研究资料均为本院于2016年4月~2018年4月期间收治的行心脏手术治疗患者,共106例,随机分组,对照组和观察组分别开展常规护理服务、全程护理服务,各53例,评价两组焦虑、抑郁情绪改善效果,观察术中应激状况及护理后护理满意度,并进行比较。结果术前1d,出院时观察组焦虑、抑郁情绪评分逐渐降低,且均显著低于同时间段对照组,P0.05;术中两组患者心率、肾上腺激素、收缩压、舒张压等指标均发生波动,其中观察组显著低于对照组,P<0.05;观察组护理满意度为96.23%,与对照组77.36%比较明显更高,P<0.05。结论针对开展心脏手术患者实施全程护理干预利于有效调节其焦虑、抑郁情绪,利于其稳定心率、血压,控制应激反应,护理服务满意度高。

关键词：全程护理模式心脏手术焦虑情绪抑郁情绪应激状况

原发性高血压患者高尿酸血症患病率及其影响因素分析

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临床医学研究与实践 2024年第14期9卷 17-20页

作者：朱倩茹邹武松江汉大学湖北武汉430056 武汉市第四医院湖北武汉430000

目的调查原发性高血压患者高尿酸血症(HUA)的患病情况并分析其可能的影响因素。方法选取2022年9月至12月在武汉市第四医院住院治疗的265例原发性高血压患者进行横断面研究。按血清尿酸(SUA)水平将其分为HUA组和非HUA组,分析原发性高血... 详细信息

目的调查原发性高血压患者高尿酸血症(HUA)的患病情况并分析其可能的影响因素。方法选取2022年9月至12月在武汉市第四医院住院治疗的265例原发性高血压患者进行横断面研究。按血清尿酸(SUA)水平将其分为HUA组和非HUA组,分析原发性高血压患者发生HUA的可能影响因素。结果265例原发性高血压患者的HUA总患病率为30.19%,男性患病率高于女性(P<0.001)。不同年龄段原发性高血压患者的HUA患病率比较,差异有统计学意义(P<0.001)。两组年龄、性别、体质指数(BMI)、BMI分级、腰围、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)水平比较,差异有统计学意义(P<0.05)。Spearman相关性检验显示,原发性高血压患者的SUA水平与HDL-C呈负相关,与TG、总胆固醇(TC)呈正相关(P<0.05)。Logistic回归分析结果显示,年龄、性别、BMI和TG是原发性高血压患者发生HUA的影响因素(P<0.05)。结论原发性高血压患者HUA患病率较高,且存在性别差异;影响原发性高血压患者发生HUA的因素有年龄、性别、BMI和TG。

关键词：高血压高尿酸血症患病率影响因素

部分突发公共卫生事件监测与现场处置关键技术遴选研究

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中华流行病学杂志 2017年第6期38卷 789-793页

作者：朱茜如杨磊马海燕谢文倩丛黎明许亮文杭州师范大学医学院 310036 浙江省疾病预防控制中心杭州310051

目的探索传染病、食物中毒、医院感染这三类突发公共卫生事件监测与现场处置中具有重要作用且需要完善与发展的关键技术，有效提高突发公共卫生事件监测与现场处置能力。方法在文献研究与专家小组讨论的基础上构建传染病、食物中毒、... 详细信息

目的探索传染病、食物中毒、医院感染这三类突发公共卫生事件监测与现场处置中具有重要作用且需要完善与发展的关键技术，有效提高突发公共卫生事件监测与现场处置能力。方法在文献研究与专家小组讨论的基础上构建传染病、食物中毒、医院感染监测与现场处置关键技术遴选框架，运用德尔菲专家咨询法以及逼近理想解排序法综合评价法，遴选三类事件监测与现场处置的关键技术。结果构建了基于事件类别、事件处理周期、技术层次的关键技术遴选框架，选择来自公共卫生领域的26位专家进行2轮专家咨询，专家权威程度为0.796；遴选出对传染病、食物中毒、医院感染监测与现场处置具有重要意义的各10项关键技术，其中，预警制度是对三类事件均具有重要意义的关键技术，未知病原微生物检测、个人防护、基因测序与溯源、微生物分子分型和核酸检测技术是传染病与医院感染监测与现场处置均需要发展的关键技术，现场快速检测、预警预测以及数据安全与保密技术是传染病与食物中毒监测与现场处置均需要完善的关键技术。此外，传染病监测与现场处置的关键技术还包括数据交换，食物中毒监测与现场处置的关键技术还包括毒素检测等，医院感染监测与现场处置的关键技术还包括数据采集标准化等。结论本研究通过科学的方法遴选出符合传染病、食物中毒、医院感染监测与现场处置需求的关键技术，在2轮专家咨询中，专家意见的权威程度较高，可保证遴选结果的可靠性，为关键技术的进一步研究与优先发展提供了科学依据。

关键词：传染病食物中毒医院感染监测现场处置

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基于兴趣导向的分析化学课程改革方法初探

山东化工 2019年第18期48卷 244-245页

作者：何梦媛朱倩茹尚宁信阳师范学院化学化工学院

作为高等院校中化学及相关专业的一门重要的基础课,学生对分析化学课程的理解和掌握情况与后续课程的学习密切相关。只有充分激发学生的学习兴趣,提高学生自主学习能力,才能加强学生对分析化学相关理论和实践操作技能的掌握,本文就分析... 详细信息

作为高等院校中化学及相关专业的一门重要的基础课,学生对分析化学课程的理解和掌握情况与后续课程的学习密切相关。只有充分激发学生的学习兴趣,提高学生自主学习能力,才能加强学生对分析化学相关理论和实践操作技能的掌握,本文就分析化学课程教学方法和考核方式的改革进行了初步探究。

关键词：兴趣导向分析化学课程改革