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High-dose dexamethasone regulates microglial polarization via the GR/JAK1/STAT3 signaling pathway after traumatic brain injury

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Neural Regeneration Research 2025年第9期20卷 2611-2623页

作者： Mengshi Yang Miao Bai Yuan Zhuang Shenghua Lu qianqian ge Hao Li Yu Deng Hongbin Wu Xiaojian Xu Fei Niu Xinlong Dong Bin Zhang Baiyun Liu Beijing Tiantan Hospital Capital Medical UniversityBeijingChina Department of Neurology The First Hospital of Tsinghua UniversityBeijingChina Department of Neurotrauma and Beijing Key Laboratory of Central Nervous System Injury Beijing Neurosurgical InstituteCapital Medical UniversityBeijingChina Department of Critical Care Medicine Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Center for Nerve Injury and Repair Beijing Institute of Brain DisordersChina National Clinical Research Center for Neurological DiseasesBeijingChina

Although microglial polarization and neuroinflammation are crucial cellular responses after traumatic brain injury,the fundamental regulatory and functional mechanisms remain insufficiently *** potent anti-inflammato ry agents,the use of glucoco rticoids in traumatic brain injury is still controversial,and their regulatory effects on microglial polarization are not yet *** the present study,we sought to determine whether exacerbation of traumatic brain injury caused by high-dose dexamethasone is related to its regulatory effects on microglial polarization and its mechanisms of *** vitro cultured BV2 cells and primary microglia and a controlled cortical impact mouse model were used to investigate the effects of dexamethasone on microglial ***,dexamethasone,RU486(a glucocorticoid receptor antagonist),and ruxolitinib(a Janus kinase 1 antagonist)were ***-sequencing data obtained from a C57BL/6 mouse model of traumatic brain injury were used to identify potential targets of *** Morris water maze,quantitative reverse transcription-polymerase chain reaction,western blotting,immunofluorescence and confocal microscopy analysis,and TUNEL,Nissl,and Golgi staining were performed to investigate our ***-throughput sequencing results showed that arginase 1,a marker of M2 microglia,was significantly downregulated in the dexamethasone group compared with the traumatic brain injury group at3 days post-traumatic brain *** dexamethasone inhibited M1 and M2 microglia,with a more pronounced inhibitory effect on M2microglia in vitro and in *** receptor plays an indispensable role in microglial polarization after dexamethasone treatment following traumatic brain ***,glucocorticoid receptor activation increased the number of apoptotic cells and neuronal death,and also decreased the density of dendritic spines.A possible downstream receptor signaling mechanism is th

关键词： apoptosis BV2 microglia dexamethasone glucocorticoid receptor glucocorticoids innate immune system microglial polarization neuroinflammation primary microglia traumatic brain injury

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Exposure to Prorocentrum minimum Induces Oxidative Stress and Apoptosis in the Ridgetail White Prawn, Exopalaemon carinicauda

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Journal of Ocean University of China 2019年第3期18卷 727-734页

作者： MU Cuimin ge qianqian LI Jian Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China

Prorocentrum minimum is a bloom-forming, planktonic mixotrophic dinoflagellate, and can cause stress in shrimp ponds. In this study, healthy Exopalaemon carinicauda were exposed to 5 × 10^4 cells mL^-1 P. minimum for 72 hours to investigate the adverse effect of P. minimum on shrimps. Elevated superoxide dismutase(SOD) activity and malondialdehyde(MDA) content, reduced total antioxidant capacity(T-AOC) and catalase(CAT) activity, and regulatory glutathione peroxidase(GPX) activity were found in the hemolymph of E. carinicauda after exposure to P. minimum. In this study, P. minimum exposure induced oxidative stress and caused significant oxidative damage to E. carinicauda. P. minimum exposure increased the expression of HSP70 gene in the hemocyte, gills and hepatopancreas. Compared with the enhanced level of caspase-3 gene mR NA in the hemocyte and gills, the up-regulation of caspase-3 gene in the hepatopancreas was only observed from 3 to 6 h, and then the mRNA level of glutathione-S-transferase(GST) gene increased. These results indicated that GST might be involved in the shrimp hepatopancreas’ defense against P. minimum exposure. The present study demonstrates that exposure to P. minimum could induce oxidative stress and apoptosis in E. carinicauda. The SOD activity, HSP70 and GST(in the hepatopancreas) were evoked to protect cells from oxidative stress and apoptosis. This study will provide new insights into the toxic mechanism of P. minimum on shrimps.

关键词： Prorocentrum minimum Exopalaemon carinicauda oxidative stress apoptosis HSP70

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Comparison of White Spot Syndrome Virus Infection Resistance Between Exopalaemon carinicauda and Litopenaeus vannamei Under Different Salinity Stresses

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Journal of Ocean University of China 2017年第6期16卷 1195-1205页

作者： ge qianqian YU ge SUN Ming LI Jitao LI Jian Key Laboratory of Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology

Exopalaemon carinicauda is one of the important economic shrimp species in China, and can tolerate a wide range of salinities. However, its disease resistance remains to be unclear in comparison with other shrimp species under salinity stress. In this study, the resistance to white spot syndrome virus(WSSV) of E. carinicauda and Litopenaeus vannamei was determined by comparing their hemocyanin(Hc) and phenoloxidase(PO) activities under different salinity stresses. In E. carinicauda, the PO activity and Hc gene transcript abundance showed a coherent pattern of increase and decrease while Hc content showed a slightly decrease with Vibrio anguillarum and WSSV infections. For both E. carinicauda and L. vannamei under salinity stress, the PO activity showed a positive correlation with the salinity while the Hc content and expression level of its gene increased significantly in salinities of 5, 15 and 25 g L^(-1). The survival rate of E. carinicauda with WSSV infection was higher than that of L. vannamei in the first 24 h under different salinity stresses. Drastic mortality of *** and L. vannamei appeared at 48 h and 3 h post-injection, respectively. Furthermore, compared with L. vannamei, E. carinicauda displayed higher PO activity, Hc content and abundance of Hc gene m RNA. The results collectively indicated that Hc and PO have obviously functional connection in resisting pathogens and tolerating salinity stress, and PO activity and Hc gene m RNA abundance may reflect the resistance of shrimp to disease. E. carinicauda has higher level of immune potential than L. vannamei, suggesting its greater capacity in resisting pathogens under salinity stresses.

关键词： Exopalaemon carinicauda Litopenaeus vannamei hemocyanin phenoloxidase activity WSSV salinity stress

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Effects of acute salinity stress on the survival and prophenoloxidase system of Exopalaemon carinicauda

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Acta Oceanologica Sinica 2020年第4期39卷 57-64页

作者： qianqian ge Zhengdao Li Jitao Li Jiajia Wang Jian Li Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture/Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The ridgetail white prawn Exopalaemon carinicauda is a euryhaline shrimp species in the estuarine and coastal areas of *** this study,survival rates,transcription levels of two prophenoloxidase system-related genes(Ec LGBP and Ecpro PO)and PO activity were determined quantitatively in juvenile and adult *** under different salinity *** results showed that *** juveniles could survive in a wider range of salinity conditions than *** juvenile ***,the expression levels of Ec LGBP and Ec Pro PO were upregulated in low salinities and showed no significant difference at 20–40,while PO activities in low salinities were higher compared to those in high *** adult ***,the expression profiles of Ec LGBP and Ecpro PO had a different trend of up-regulation in salinity stress treatments and no obvious difference was observed in the gene expression levels and PO activity between 30 and *** salinity tolerance range of immunity for juvenile and adult *** is 20–40 and 30–40,respectively.

关键词： Exopalaemon carinicauda prophenoloxidase system salinity stress immunity tolerant range

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Antioxidant Response of Ridgetail White Prawn Exopalaemon carinicauda to Harmful Dinoflagellate Prorocentrum minimum Exposure and Its Histological Change

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Journal of Ocean University of China 2017年第2期16卷 285-293页

作者： MU Cuimin REN Xianyun ge qianqian WANG Jiajia LI Jian Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology

The dinoflagellate Prorocentrum minimum,one of the most widespread red tide causing species,affects marine aquaculture and ecosystems *** this study,ridgetail white prawn Exopalaemon carinicauda were exposed to *** cells(5 × 104 cells mL-1) to investigate its harmful effects on the *** activities and histological changes were used as indicators of health status of the *** 72 hours,the mortality of *** was not affected,but its antioxidant response and histology were statistically different from those of *** superoxide dismutase(SOD) and glutathione peroxidase(GPX) activities and depressed catalase(CAT) activity were observed in gill;while increased SOD,glutathione S-transferase(GST),CAT activities and modulated GPX activity were observed in ***,antioxidant activities in gill and hepatopancreas seem to respond differentially to harmful alga *** malondialdehyde(MDA) content in early a few hours indicates the damage of the antioxidant defense *** MDA content recovered to a low level thereafter,a series of histological abnormalities including accumulation or infiltration of hemocytes,tissue lesions and necrosis were discovered in gill and *** to *** induced sublethal effects on ***,including temporary oxidative damage and histological injury.

关键词： Prorocentrum minimum Exopalaemon carinicauda antioxidant response histological changes

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Isolation of Prawn(Exopalaemon carinicauda) Lipopolysaccharide and β-1,3-Glucan Binding Protein gene and Its Expression in Responding to Bacterial and Viral Infections

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Journal of Ocean University of China 2016年第2期15卷 288-296页

作者： ge qianqian LI Jian DUAN Yafei LI Jitao SUN Ming ZHAO Fazhen Fisheries College Ocean University of China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization of Ministry of Agriculture South China Sea Fisheries Research InstituteChinese Academy of Fishery Sciences

The pattern recognition proteins （PRPs） play a major role in immune response of crustacean to resist pathogens. In the present study, as one of PRPs, lipopolysaccharide and 13-1, 3-glucan binding protein （LGBP） gene in the ridge tail white prawn （Exopalaemon carinicauda）（EcLGBP） was isolated. The full-length cDNA of EcLGBP was 1338 bp, encoding a polypeptide of 366 amino acid residules. The deduced amino acid sequence of EcLGBP shared high similarities with LGBP and BGBP from other crus- taceans. Some conservative domains were predicted in EcLGBP sequence. EcLGBP constitutively expressed in most tissues at dif- ferent levels, and the highest expression was observed in hepatopancreas. With infection time, the cumulative mortality increased gradually followed by the proliferation of Vibrio parahaemolyticus and white spot syndrome virus （WSSV）. The expression of EcLGBP in response to E parahaemolyticus infection was up-regulated in hemocytes and hepatopancreas, and the up-regulation in hepatopancreas was earlier than that in hemocytes. EcLGBP expression after WSSV infection increased at 3 h, then significantly decreased in both hemocytes and hepatopancreas. The results indicated that EcLGBP was involved in the immune defense against bacterial and viral infections.

关键词： Exopalaemon carinicauda LGBP WSSV Vibrio parahaemolyticus immune response

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Ecosystem service values of gardens in the Yellow River Basin,China

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Journal of Arid Land 2022年第3期14卷 284-296页

作者： ge qianqian XU Wenjie FU Meichen HAN Yingxin AN Guoqiang XU Yuetong School of Land Science and Technology China University of Geosciences(Beijing)Beijing 100083China China Land Surveying and Planning Institute Beijing 100035China Shandong Urban and Rural Planning Design Institute Jinan 250013China Territorial Spatial Planning Institute of Shandong Province Jinan 250014China College of geography and Environment Shandong Normal UniversityJinan 250358China Key Laboratory of Land Use Ministry of Natural ResourcesShandong BasisJinan 250014China

Studies on the ecosystem service value(ESV)of gardens are critical for informing evidence-based land management practices based on an understanding of the local *** analyzing equivalent value factors(EVFs),this paper evaluated the values of 11 ecosystem services of gardens in the Yellow River Basin of China in ***-precision land use survey data were used to improve the accuracy of the land use classification,garden areas,and spatial distribution of the ESVs of *** results showed that garden ecosystem generally had high ESVs,especially in terms of the ESV of food production,which is worthy of further research and application to the practice of land use planning and ***,the value of one standard EVF of ecosystem services in 2019 was 3587.04 CNY/(hm^(2)·a),and the ESV of food production of gardens was much higher than that of *** ecosystem provided an ESV of 1348.66×10^(8)CNY/a in the Yellow River *** areas with the most concentrated ESVs of gardens were located in four regions:downstream in the Shandong-Henan zone along the Yellow River,mid-stream in the Shanxi-Shaanxi zone along the Yellow River,the Weihe River Basin,and upstream in the Qinghai-Gansu-Ningxia-Inner Mongolia zone along the Yellow *** spatial correlation of the ESVs in the basin was significant(global spatial autocorrelation index Moran's I=0.464),which implied that the characteristics of high ESVs adjacent to high ESVs and low ESVs adjacent to low ESVs are *** the Yellow River Basin,the contribution of the ESVs of gardens to the local environment and economy varied across *** also put forward some suggestions for promoting the construction of ecological civilization in the Yellow River *** findings of this study provide important contributions to the research of ecosystem service evaluation in the Yellow River Basin.

关键词： ecosystem service evaluation garden ecosystem equivalent value factors spatial autocorrelation analysis Yellow River Basin

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Pathological Networks Involving Dysmorphic Neurons in Type ⅡFocal Cortical Dysplasia

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Neuroscience Bulletin 2022年第9期38卷 1007-1024页

作者： Yijie Shao qianqian ge Jiachao Yang Mi Wang Yu Zhou Jin-Xin Guo Mengyue Zhu Jiachen Shi Yiqi Hu Li Shen Zhong Chen Xiao-Ming Li Jun-Ming Zhu Jianmin Zhang Shumin Duan Jiadong Chen Center for Neuroscience and Department of Neurosurgery of the Second Affiliated Hospital NHC and CAMS Key Laboratory of Medical NeurobiologyMOE Frontier Science Center for Brain Research and Brain-Machine IntegrationSchool of Brain Science and Brain MedicineZhejiang University School of MedicineHangzhou310058China Department of Neurosurgery of the Second Affiliated Hospital Zhejiang University School of MedicineHangzhou310009China The MOE Key Laboratory of Biosystems Homeostasis&Protection and Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology Life Sciences InstituteZhejiang UniversityHangzhou310058China Department of Orthopedic Surgery School of Medicinethe Second Affiliated HospitalZhejiang UniversityHangzhou310009China Hangzhou Innovation Center Zhejiang UniversityHangzhou310058China Institute of Pharmacology&Toxicology NHC and CAMS Key Laboratory of Medical NeurobiologyCollege of Pharmaceutical SciencesSchool of Basic Medical SciencesZhejiang UniversityHangzhou310058China Key Laboratory of Neuropharmacology and Translational Medicine of Zhejiang Province College of Pharmaceutical SciencesZhejiang Chinese Medical UniversityHangzhou310058China Center for Neuroscience and Department of Neurology of the Second Affiliated Hospital Zhejiang University School of MedicineHangzhou310058China NHC and CAMS Key Laboratory of Medical Neurobiology Center for Brain Science and Brain-Inspired IntelligenceJoint Institute for Genetics and Genome Medicine betweenGuangdong Hong Kong Macao Greater Bay AreaZhejiang University and the University of TorontoZhejiang University School of MedicineHangzhou310058China

Focal cortical dysplasia(FCD)is one of the most common causes of drug-resistant *** neurons are the major histopathological feature of typeⅡFCD,but their role in seizure genesis in FCD is *** we performed whole-cell patch-clamp recording and morphological reconstruction of cortical principal neurons in postsurgical brain tissue from drug-resistant epilepsy *** analyses revealed distinct morphological and electrophysiological characteristics of the upper layer dysmorphic neurons in typeⅡFCD,including an enlarged soma,aberrant dendritic arbors,increased current injection for rheobase action potential firing,and reduced action potential firing ***,the upper layer dysmorphic neurons received decreased glutamatergic and increased GABAergic synaptic inputs that were coupled with upregulation of the Na^(+)-K^(+)-Cl^(−)*** addition,we found a depolarizing shift of the GABA reversal potential in the CamKⅡ-cre::PTENflox/flox mouse model of drug-resistant epilepsy,suggesting that enhanced GABAergic inputs might depolarize dysmorphic ***,imbalance of synaptic excitation and inhibition of dysmorphic neurons may contribute to seizure genesis in typeⅡFCD.

关键词： Focal cortical dysplasia Dysmorphic neuron Whole-cell patch-clamp recording Morphological reconstruction Excitation-inhibition balance

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离子交换膜的发展态势与应用展望

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化工进展 2016年第6期35卷 1774-1785页

作者：葛倩倩葛亮汪耀明徐铜文中国科学技术大学化学与材料学院安徽合肥230026

基于离子交换膜的电膜技术,由于其独特的离子传递特性,可以用于离子物系的分离、分级,在清洁生产、节能减排、能量转换等方面有着广泛的应用前景。本文综述了离子交换膜的制备、应用过程以及组件设计等方面的前沿性进展,并对亟待解决的... 详细信息

基于离子交换膜的电膜技术,由于其独特的离子传递特性,可以用于离子物系的分离、分级,在清洁生产、节能减排、能量转换等方面有着广泛的应用前景。本文综述了离子交换膜的制备、应用过程以及组件设计等方面的前沿性进展,并对亟待解决的问题和未来的发展方向作了展望。关于膜的制备,提出了从二相到三相、从致密到微孔的新型离子交换膜结构,开发了电纳滤膜并用于一价或多价离子的分离,通量和选择性均得到提高,实现了膜功能的多样化。在应用过程中,实现了扩散渗析和电渗析过程的集成,分离效果优越,生产成本降低。同时对膜组件进行优化,设计开发出新型的卷式组件,克服了传统板式组件的诸多缺陷。值得一提的是,离子分离膜的应用领域也由初级水处理扩展到复杂物料的分离与纯化。以上研究成果将为离子交换膜的发展提供指导,加快其工业化进程。

关键词：离子交换电渗析扩散渗析电纳滤传质分离

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基于微胶囊技术的超疏水自修复涂层研究进展

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中国表面工程 2022年第4期35卷 102-112页

作者：葛倩倩鲁浈浈梁杨魏鹏重庆交通大学土木工程学院重庆400074 重庆交通大学交通土建工程材料国家地方联合工程实验室重庆400074 重庆市政设施管理局重庆400014

超疏水涂层在自清洁、防腐蚀、抗凝冰、减阻等领域具有广阔的应用前景,但其耐久性还有待提高。由于微胶囊在修复剂的封装、制备等方面具备独特优势,利用微胶囊技术将修复剂封装,均匀分散于超疏水涂层后,涂层可同时具有疏水性和自修复性... 详细信息

超疏水涂层在自清洁、防腐蚀、抗凝冰、减阻等领域具有广阔的应用前景,但其耐久性还有待提高。由于微胶囊在修复剂的封装、制备等方面具备独特优势,利用微胶囊技术将修复剂封装,均匀分散于超疏水涂层后,涂层可同时具有疏水性和自修复性,但目前对微胶囊技术与超疏水涂层结合并进行归纳总结的文献较少。总结了微胶囊技术在超疏水材料自修复领域的研究现状,归纳了微胶囊超疏水涂层自修复的修复机理与常用制备方法,对不同刺激响应方式下微胶囊超疏水涂层的性能(响应速度、自修复效果、耐久性、耐磨性)进行对比。结果表明,以紫外光照射为修复手段的涂层机械耐久性较好,近红光刺激响应是微胶囊超疏水自修复涂层中响应修复效率最快、修复效果最好的修复方式,并且以紫外光和近红光双重刺激响应的超疏水涂层的耐久性、耐化学腐蚀性远超过单一刺激响应型超疏水自修复涂层。最后,提出当前基于微胶囊技术的超疏水自修复涂层存在的问题并对该涂层的应用前景进行展望。

关键词：新材料微胶囊超疏水涂层自修复刺激响应

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