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Single-cell analyses reveal cannabidiol rewires tumor microenvironment via inhibiting alternative activation of macrophage and synergizes with anti-PD-1 in colon cancer

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Journal of Pharmaceutical Analysis 2023年第7期13卷 726-744页

作者： Xiaofan Sun Lisha Zhou Yi Wang Guoliang Deng Xinran Cao Bowen Ke Xiaoqi Wu Yanhong Gu Haibo Cheng Qiang Xu qianming du Hongqi Chen Yang Sun Department of Rheumatology and Immunology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing UniversityNanjing210008China State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing210023China Colon and Rectal Surgery Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese MedicineNanjing210001China Laboratory of Anesthesia and Critical Care Medicine Department of AnesthesiologyTranslational Neuroscience CenterWest China Hospital and State Key Laboratory of BiotherapySichuan UniversityChengdu610044China Genergy Bio-technology(Shanghai)Co. LtdShanghai200241China The First Affiliated Hospital of Nanjing Medical University Jiangsu Province HospitalNanjing210029China Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine in Prevention and Treatment of Tumor The First Clinical College of Nanjing University of Chinese MedicineNanjing210023China General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjing210006China School of Basic Medicine&Clinical Pharmacy China Pharmaceutical UniversityNanjing210009China Department of General Surgery Shanghai Jiao Tong University Affiliated Sixth People's HospitalShanghai200030China Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy Xuzhou Medical UniversityXuzhouJiangsu221004China

Colorectal tumors often create an immunosuppressive microenvironment that prevents them from responding to ***(CBD)is a non-psychoactive natural active ingredient from the cannabis plant that has various pharmacological effects,including neuroprotective,antiemetic,anti-inflammatory,and antineoplastic *** study aimed to elucidate the specific anticancer mechanism of CBD by single-cell RNA sequencing(scRNA-seq)and single-cell ATAC sequencing(scATAC-seq)***,we report that CBD inhibits colorectal cancer progression by modulating the suppressive tumor microenvironment(TME).Our single-cell transcriptome and ATAC sequencing results showed that CBD suppressed M2-like macrophages and promoted M1-like macrophages in tumors both in strength and ***,CBD significantly enhanced the interaction between M1-like macrophages and tumor cells and restored the intrinsic anti-tumor properties of macrophages,thereby preventing tumor ***,CBD altered the metabolic pattern of macrophages and related anti-tumor signaling *** found that CBD inhibited the alternative activation of macrophages and shifted the metabolic process from oxidative phosphorylation and fatty acid oxidation to glycolysis by inhibiting the phosphatidylinositol 3-kinase-protein kinase B signaling pathway and related downstream target ***,CBD-mediated macrophage plasticity enhanced the response to anti-programmed cell death protein-1(PD-1)immunotherapy in xenografted *** together,we provide new insights into the anti-tumor effects of CBD.

关键词： scRNA-seq scATAC-seq Cannabidiol Colorectal cancer Tumor microenvironment Macrophage

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精胺氧化酶靶向调控硫氧还蛋白还原酶1促进结肠癌恶性进展

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实用肿瘤杂志 2024年第4期39卷 317-332页

作者：王慧刘超章春雪姚懿芹陈龙祁悦欣费禹翔赵树立胡容杜前明武汉东湖学院护理与健康管理学院(生命科学与化学学院) 湖北武汉430212 中国药科大学基础医学与临床药学学院江苏南京210009 南京医科大学附属南京医院南京市第一医院药学部江苏南京210006 中国药科大学药学院江苏南京210009 南京医科大学附属南京医院南京市第一医院临床医学研究中心实验室江苏南京210006

目的探讨精胺氧化酶(spermine oxidase,SMOX)靶向调控硫氧还蛋白还原酶1(thioredoxin reductase 1,TR1)对结肠癌恶性进展的影响。方法收集2018年9月至2019年6月就诊于南京市第一医院的30例结肠癌患者的结肠癌组织及其癌旁组织。采用免... 详细信息

目的探讨精胺氧化酶(spermine oxidase,SMOX)靶向调控硫氧还蛋白还原酶1(thioredoxin reductase 1,TR1)对结肠癌恶性进展的影响。方法收集2018年9月至2019年6月就诊于南京市第一医院的30例结肠癌患者的结肠癌组织及其癌旁组织。采用免疫组织化学染色检测组织中的SMOX表达。采用Western blot实验检测结肠癌细胞株(HCT116、SW480、DLD-1、SW620、Caco-2、HCT-15、T84和LS123)和正常结肠细胞株NCM460的SMOX表达。利用基因表达谱互作分析(Gene Expression Profiling Interactive Analysis,GEPIA)网站查询SMOX在结肠癌组织与癌旁组织中的表达情况和SMOX表达水平与患者总生存的关系。采用Western blot实验检测上述细胞和组织中代谢产物亚精胺合成酶的水平。利用2',7'-二氯二氢荧光素二乙酸酯(2',7'-dichlorodihydrofluorescein diacetate,DCFH-DA)结合流式细胞术和组织免疫荧光实验分别检测结肠细胞和组织中代谢副产物活性氧(reactive oxygen species,ROS)水平。选取SMOX表达水平最高的结肠癌细胞株HCT116和SW480为实验对象,构建慢病毒载体,用PLKO.1-puro-shCtrl、PLKO.1-puro-shSMOX、PLKO.1-puro-shSMOX+pcDNA3.1和PLKO.1-puro-shSMOX+pcDNA3.1-TR1分别转染细胞,分别为shCtrl组、shSMOX组、shSMOX+pcDNA3.1组和shSMOX+TR1组。Western blot实验检测HCT116和SW480细胞shCtrl组和shSMOX组SMOX与TR1的表达情况,以及HCT116细胞shSMOX+pcDNA3.1组和shSMOX+TR1组的TR1表达水平。利用细胞计数试剂盒(Cell Counting Kit-8,CCK-8)检测细胞增殖活力。碘化丙啶(propidium iodide,PI)单染结合流式细胞术检测肿瘤细胞周期变化。PI/异硫氰酸荧光素-膜连蛋白(PI/fluorescein isothiocyanate-Annexin V,PI/FITC-Annexin V)双染结合流式细胞术检测细胞凋亡/坏死情况。Transwell体外侵袭实验分析细胞侵袭能力。shCtrl组、shSMOX组、shSMOX+pcDNA3.1组和shSMOX+TR1组的HCT116细胞稀释至浓度为1×107个/mL的细胞悬液分别向裸鼠右侧腋窝皮下注射0.2 mL细胞悬液,建立裸鼠结肠癌移植瘤模型。4周后处死裸鼠,分离组织,剥取肿瘤称重。运用免疫组织化学染色检测裸鼠结肠肿瘤组织中Ki-67阳性细胞数。结果SMOX在8种结肠癌细胞株和结肠癌组织中的表达水平均高于正常结肠细胞株和癌旁组织,且与不良预后有关(均P<0.05)。与正常结肠细胞和癌旁组织比较,8种结肠癌细胞和结肠癌组织中的代谢产物亚精胺合成酶和ROS水平随SMOX的高表达同步升高(均P<0.05)。与shCtrl组比较,shSMOX组G_(0)/G_(1)期细胞数目增多,PI/Annexin V双阳性细胞数目增加,细胞增殖活性降低,侵袭数量减少,SMOX和TR1表达水平降低(均P<0.05)。与shSMOX组和shSMOX+pcDNA3.1组比较,shSMOX+TR1组TR1表达水平升高,S期细胞数目增多,PI/Annexin V双阳性细胞数目减少,细胞增殖活性升高(均P<0.05)。裸鼠结肠癌移植瘤模型显示,注射后14 d,与shCtrl组比较,shSMOX组和shSMOX+pcDNA3.1组肿瘤体积和重量均降低,结肠肿瘤组织中Ki-67阳性细胞数减少,而shSMOX+TR1组肿瘤体积、重量和结肠肿瘤组织中Ki-67阳性细胞数则较shSMOX+pcDNA3.1组增加(均P<0.05)。结论SMOX在结肠癌细胞和组织中表达上调,可能通过靶向提高TR1的表达促进细胞周期运转,抑制细胞凋亡,促进细胞增殖和侵袭能力及肿瘤生长,进而促进结肠癌恶性进展。

关键词：结肠癌精胺氧化酶硫氧还蛋白还原酶1 恶性进展

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大数据时代高职数学课程教学改革研究

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科教导刊 2025年第1期 21-23页

作者：高前明江苏财经职业技术学院江苏淮安223003

文章通过分析当前高职数学教学的现状与挑战,提出了基于大数据的高职数学教学改革策略,包括数据驱动的课程设计、个性化学习路径的构建以及动态评价体系建设。文章展望了高职数学教学改革在大数据时代的发展趋势,旨在为高职数学教育工... 详细信息

文章通过分析当前高职数学教学的现状与挑战,提出了基于大数据的高职数学教学改革策略,包括数据驱动的课程设计、个性化学习路径的构建以及动态评价体系建设。文章展望了高职数学教学改革在大数据时代的发展趋势,旨在为高职数学教育工作者提供有益的参考与启示。

关键词：大数据高职数学教学教学改革数据驱动个性化学习

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The Effect of Geologic Structures on the Control of Floods in the Middle Yangtze River Valley

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Acta Geologica Sinica(English Edition) 2000年第2期74卷 344-348页

作者： Tong qianming Hunan Institute of Geology 389 Furong ZhongluChangsha 410007Hunan

This paper discusses the role of geologic structures in the occurrence of floods and how to prevent flood in the middle reaches of the Yangtze River, and gives the author's suggestion that the Luoshan Qiakou be expand... 详细信息

关键词： the middle Yangtze River valley structures flood flood control

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The cytokine network involved in the host immune response to periodontitis

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International Journal of Oral Science 2019年第4期11卷 276-288页

作者： Weiyi Pan Qingxuan Wang qianming Chen State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Chinese Academy of Medical Sciences Research Unit of Oral Carcinogenesis and Management West China Hospital of Stomatology Sichuan University

Periodontitis is an inflammatory disease involving the destruction of both soft and hard tissue in the periodontal *** dysbiosis of the local microbial community initiates local inflammation,over-activation of the host immune response directly activates osteoclastic activity and alveolar bone *** studies have reported on the cytokine network involved in periodontitis and its crucial and pleiotropic effect on the recruitment of specific immunocytes,control of pathobionts and induction or suppression of osteoclastic ***,particularities in the stimulation of pathogens in the oral cavity that lead to the specific and complex periodontal cytokine network are far from ***,in this review,we begin with an up-to-date aetiological hypothesis of periodontal disease and summarize the roles of cytokines in the host immune *** addition,we also summarize the latest cytokine-related therapeutic measures for periodontal disease.

关键词： cytokine stimulation inflammation

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Overview of power quality analysis and control technology for smart grid

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Journal of Modern Power Systems and Clean Energy 2016年第1期4卷 1-9页

作者： An LUO qianming XU Fujun MA Yandong CHEN National Electric Power Conversion and Control Engineering Technology Research Center(Hunan University) ChangshaChina

With the wide application of non-linear loads and the large-scale access of distributed energy generations based on power electronics equipments, power quality problems in the distribution network are increasingly serious with new characteristics. Further in-depth research is of great significance in theory and practice. This paper provides an overview of power quality analysis, compensators, and control technologies under the new situation of smart grid. It focuses on the topologies and control methods for power quality conditioners, especially new characteristics of power quality and applicable control technologies in microgrids and distributed power ***, trends and prospects of power quality control technology are introduced, which is important to achieve security and efficient operation of smart grid.

关键词： Power quality Smart grid Active control Passive control

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In-situ TEM investigation of MoS2 upon alkali metal intercalation

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Science China Chemistry 2018年第2期61卷 222-227页

作者： qianming Huang Lifen Wang Zhi Xu Wenlong Wang Xuedong Bai Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190 China Collaborative Innovation Center of Quantum Matter Beijing 100190 China School of Physical Sciences University of Chinese Academy of Sciences Beijing 100190 China

Phase transition in two dimensional molybdenum disulfide (MoS_2) can be induced by several methods and has been investigated for decades. Alkali metal insertion of MoS_2 had been proved an effective method to cause phase transition early in 1970s, and has been gaining renewed interest recently, due to the possible application of MoS_2 in energy storage. The alkali metal intercalation of MoS_2 has been studied by various techniques, among which in-situ transmission electron microscopy (TEM) provides unique capability of real time resolving the structural evolution of the materials at high spatial resolutions. Here by in-situ TEM technique we investigated the structural evolution of MoS_2 upon lithium and sodium intercalation, along with transformation of the nanosheet and variation of the electron diffraction patterns. The intercalation process is accompanied by emergence of superstructures, which exist in several forms. The ion intercalation results in phase transition of MoS_2 from 2H to 1T, and the driving mechanism of the phase transition are discussed. The work provides a more comprehensive understanding of ion intercalation induced phase transition of MoS_2.

关键词： molybdenum disulfide phase transition intercalation in-situ TEM

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聚落“双修”视域下传统村落的活态性及保护探索——以陇中黄土丘陵沟壑区古城村为例

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地域研究与开发 2025年第1期44卷 130-136页

作者：薛乾明宁雷兰州交通大学建筑与城市规划学院兰州730070 西昌学院旅游与城乡规划学院四川西昌615000

以陇中黄土丘陵沟壑区甘肃省榆中县古城村为例,通过田野调查,构建其活态性评价指标体系并对其进行类型划分,通过引入聚落“双修”理念,针对性地对村落的保护发展路径进行探索。结果表明:(1)当前村落处于失活状态,受地域生态环境、本土... 详细信息

以陇中黄土丘陵沟壑区甘肃省榆中县古城村为例,通过田野调查,构建其活态性评价指标体系并对其进行类型划分,通过引入聚落“双修”理念,针对性地对村落的保护发展路径进行探索。结果表明:(1)当前村落处于失活状态,受地域生态环境、本土文化保护、村落经济活力、保护发展机制及村民意愿诉求5类因子综合影响显著,其因子得分越高,村落的活态化发展水平越高。(2)影响村落活态发展的指标因素中,历史文化、村民诉求两个因素影响最大,而生态环境、经济产业及保护机制3个因素次之。(3)依据指标体系和村落现实困境,优先从生态、文化、经济、机制以及人口5个方面因地制宜地制定当前地区村落保护发展的路径策略。

关键词：聚落双修传统村落保护路径评价指标体系活态性

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Dental-craniofacial manifestation and treatment of rare diseases

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International Journal of Oral Science 2019年第1期11卷 10-24页

作者： En Luo Hanghang Liu Qiucheng Zhao Bing Shi qianming Chen State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology Sichuan University

Rare diseases are usually genetic, chronic and incurable disorders with a relatively low incidence. Developments in the diagnosis and management of rare diseases have been relatively slow due to a lack of sufficient profit motivation and market to attract research by companies. However, due to the attention of government and society as well as economic development, rare diseases have been gradually become an increasing concern. As several dental-craniofacial manifestations are associated with rare diseases,we summarize them in this study to help dentists and oral maxillofacial surgeons provide an early diagnosis and subsequent management for patients with these rare diseases.

关键词： usually genetic sufficient profit

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Diagnostic strategies for diseases with fever in dental clinics

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第4期24卷 352-358页

作者： Jian YUAN Chuanxia LIU Zaiye LI qianming CHEN Stomatology Hospital School of StomatologyZhejiang University School of MedicineClinical Research Center for Oral Diseases of Zhejiang ProvinceKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityHangzhou 310006China Department of Oral Medicine West China Hospital of StomatologySichuan UniversityState Key Laboratory of Oral DiseasesNational Clinical Research Center for Oral DiseasesChengdu 610041China

Fever is an increase in body temperature beyond the normal range,acting as a protective inflammatory *** article summarizes diseases with fever encountered in dental clinics,including what is known about pyrexia in coronavirus infection,and further proposes a“six steps in one”identification and analysis strategy to guide the clinical work of *** to the mechanism of occurrence,fever is divided into pyrogenic and nonpyrogenic types.

关键词： fever mechanism diseases

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