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Effects of resistin-like molecule β over-expression on gastric cancer cells in vitro

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World Journal of Gastroenterology 2012年第8期18卷 754-766页

作者： Li-Duan Zheng Ling tong Chun-Lei Yang Teng Qi Meng Qi qiang-song tong Department of Pathology Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Department of Surgery Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China

AIM: To investigate the effects of resistin-like molecule β (RELMβ) over-expression on the invasion, metastasis and angiogenesis of gastric cancer cells. METHODS: Human RELMβ encoding expression vec tor was constructed and transfected into the RELMβ lowly-expressed gastric cancer cell lines SGC7901 and MKN-45. Gene expression was measured by Western blotting, reverse transcription polymerase chain reaction (PCR) and real-time quantitative PCR. Cell proliferation was measured by 2-(4,5-dimethyltriazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetry, colony formation and 5-ethynyl-20-deoxyuridine incorporation assays. The in vitro migration, invasion and metastasis of cancer cells were measured by cell adhesion assay, scratch assay and matrigel invasion assay. The angiogenic capabilities of cancer cells were measured by tube formation of endothelial cells. RESULTS: Transfection of RELMβ vector into SGC-7901 and MKN-45 cells resulted in over-expression of RELMβ, which did not infl uence the cellular proliferation. However, over-expression of RELMβ suppressed the in vitro adhesion, invasion and metastasis of cancer cells, accompanied by decreased expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. Moreover, transfection of RELMβ attenuated the expression of vascular endothelial growth factor and in vitro angiogenic capabilities of cancer cells. CONCLUSION: Over-expression of RELMβ abolishes the invasion, metastasis and angiogenesis of gastric cancer cells in vitro, suggesting its potentials as a novel therapeutic target for gastric cancer.

关键词： Resistin-like molecule β Gastric cancer Invasion Metastasis Angio-genesis

Risk stratifi cation and therapeutics of neuroblastoma:the challenges remain

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World Journal of Pediatrics 2016年第1期12卷 5-7页

作者： Hong Mei Zhen-Yu Lin qiang-song tong Department of Pediatric Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Cancer Center Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

Pediatric oncology is a rapidly evolving and exciting *** advances have been made in this field through the integration of systematic basic laboratory and clinical research,treatment outcomes are still suboptimal for patients with a malignant solid ***,the most common extracranial solid tumor of childhood,accounts for 7%-8%of all cancers in children in the United States and United Kingdom.[1,2]It typically originates from the adrenal gland,sympathetic ganglia。

关键词： neuroblastoma clinical sympathetic

Stable knockdown of heparanase expression in gastric cancer cells in vitro

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World Journal of Gastroenterology 2009年第43期15卷 5442-5448页

作者： Li-Duan Zheng Guo-song Jiang Jia-Rui Pu Hong Mei Ji-Hua Dong Xiao-Hua Hou qiang-song tong Department of Pathology Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Department of Surgery Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Department of Central Laboratory Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Department of Gastroenterology Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China

AIM:To develop short hairpin RNA(shRNA)against heparanase,and to determine its effects on heparanase expression and the malignant characteristics of gastric cancer cells. METHODS:Heparanase-specific shRNA was constructed and transferred into cultured the gastric cancer cell line *** subclonal cells were screened by G418 *** expression was measured by reverse transcriptase-polymerase chain reaction(RT-PCR),real-time quantitative PCR and Western *** proliferation was detected by 2-(4,5-dimethyltriazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT)colorimetry and colony formation assay. The in vitro invasiveness and metastasis of cancer cells were measured by cell adhesion assay,wound healingassay and matrigel invasion *** angiogenesis capabilities of cancer cells were measured by tube formation of endothelial cells. RESULTS:Stable transfection of heparanase-specific shRNA,but not of scrambled shRNA and mock vector,resulted in reduced mRNA and protein levels of *** shRNA-mediated knockdown of heparanase did not affect the cellular proliferation of SGC-7901 ***,the in vitro invasiveness and metastasis of cancer cells were decreased after knockdown of ***,transfection of heparanase-specific shRNA decreased the in vitro angiogenesis capabilities of SGC-7901 cells. CONCLUSION:Stable knockdown of heparanase can efficiently decrease the invasiveness,metastasis and angiogenesis of human gastric cancer *** contrast,stable knockdown of heparanase does not affect the cell proliferation.

关键词： Gastric cancer Heparanase RNA interference Invasion Metastasis

Helicobacter pylori Infection Facilitates the Expression of Resistin-like Molecule Beta in Gastric Carcinoma and Precursor Lesions

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Current Medical Science 2020年第1期40卷 95-103页

作者： Hong-jun LI Er-hu FANG Jian-qun WANG Li-duan ZHENG qiang-song tong Department of Pathology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Helicobacter pylori(***)was reported to be associated with gastric ***-like molecule beta(RELMβ),a recently described goblet cell-specific protein,was demonstrated to aberrantly express in gastric cancer and correlated with its clinicopathological *** study aimed to examine the association between *** and RELMp expression in gastric carcinoma and precursor *** infection and RELMβexpression were immunohistochemically evaluated in gastric biopsies from 230 *** biopsies consisted of normal gastric mucosa(w=20),mucosa with chronic gastritis(n=41),intestinal metaplasia(n=42),dysplasia(w=31),intestinal-type adenocarcinoma(n=56),and diffuse-type adenocarcinoma(n=40).RELMβ expression was measured in gastric biopsies after *** eradication therapy in a subgroup of 32 *** gastric cancer cell line SGC-7901 was infected with *** strains,and RELMp expression was detected by reverse transcription PCR,real-time PCR and Western *** RELMP immunoreactivity was observed in *** intestinal metaplasia(P=0.003),dysplasia(P=0.032),intestinal-type(P=0.037)and diffuse-type adenocarcinomas(P=0.001)than in ***-negative *** rates of RELMβ in dysplasia(CO.005),intestinal-type adenocarcinoma(P<0.001),and diffuse-type adenocarcinoma(P=0.001)were significantly correlated with the grade of *** *** addition,*** eradication reduced the RELMβintensity in intestinal metaplasia(P=0.001).Infection of gastric cancer SGC-7901 cells with cag pathogenicity island(PAI)-positive *** TN2,but not with its PAI totally deleted mutant(TN2-APAI)for 4-8 h,resulted in enhanced protein and transcript levels of RELMβ(P<0.05).In summary,our study suggested that *** infection facilitated the expression of RELMβ in gastric garcinoma and precursor lesions.

关键词： resistin-like molecule beta Helicobacter pylori gastric carcinoma immunohistochemistry

Gastric carcinoma with osteoclast-like giant cells:a case report and review of the literature

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第3期10卷 237-241页

作者： Li-duan ZHENG Xiu-ping YANG Hua-xiong PAN Xiu NIE Jun HE Qing LV qiang-song tong Department of PathologyUnion Hospital of tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Department of Surgery Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China

Gastric carcinoma with osteoclast-like giant cells （OGCs） is an extremely rare tumor. So far, only six cases have been reported in the literature. Here we report an additional case of this tumor in a Chinese 78-year-old man presented with abdominal pain, vomiting, and hematemesis. Physical examination and gastroscopy revealed a tumor in the gastric antrum. The biopsy and pathological findings indicated a gastric adenocarcinoma with OGCs, which were present in both the tumor and the metastatic lymph nodes. Further immunohistochemical staining indicated that OGCs were reactive with CD68, CD45, and vimentin protein, but not with pancytokeratin, carcinoembryonic antigen, or epithelial membrane antigen, suggesting the monocytic/histiocytic derivation of these OGCs. In situ hybridization for Epstein-Burr virus showed no nuclear positivity in either adenocarcinoma or OGCs. Postoperative follow-up showed that the patient had survived for at least 6 months without recurrence. Further investigation is warranted to clearly define the prognostic significance of OGCs in gastric carcinoma.

关键词： Gastric carcinoma Adenocarcinoma Osteoclast-like giant cell (OGC)

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Nitrofen suppresses cell proliferation and promotes mitochondria-mediated apoptosis in type Ⅱ pneumocytes

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Acta Pharmacologica Sinica 2007年第5期28卷 672-684页

作者： qiang-song tong~(2,5),Li-duan ZHENG~3,Shao-tao TANG~2,Guo-song JIANG~2,Qing-lan RUAN~2,Fu-qing ZENG~2,Ji-hua DONG~4~2 Department of Surgery,~3 Department of Pathology,and ~4 Department of Central Laboratory,Union Hospital of tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China Department of Surgery Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Pathology Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Central Laboratory Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To characterize the molecular mechanisms of nitrofen-induced ***:After administration of nitrofen to cultured type Ⅱ A549pneumocytes,cell proliferation and DNA synthesis were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetry,colony forma-tion assay,flow cytometry and[~3H]-thymidine incorporation *** measured by terminal transferase-mediated dUTP nick-end-labeling,acridineorange-ethidium bromide staining and flow *** of proliferatingcell nuclear antigen (PCNA) and apoptosis-related genes was assayed byimmunofluorescence,RT-PCR and Western ***:Nitrofen inhibited thecell proliferation of A549 cells in a dose-and time-dependent manner,accompa-nied by downregulation of *** a result,the DNA synthesis of nitrofen-treated A549 cells decreased,while cell cycle was arrested at G0/***,nitrofen induced apoptosis of A549 cells,which was not abolished by Z-Val-Ala-Asp(OCH3)-*** addition,nitrofen decreased the expressionof Bcl-xL,but not of Bcl-2,Bax,and Bak,resulting in a loss of mitochondrialmembrane potential and the nuclear translocation of apoptosis-inducing factor(AIF).Meanwhile,nitrofen strongly activated the p38 mitogen-activated proteinkinase (p38-MAPK).Pretreatment of cells with SB203580 (5 μmol/L) blockednitrofen-induced phosphorylation of p38-MAPK and abolished nitrofen-inducedAIF translocation and apoptosis in A549 ***:Nitrofen suppressesthe proliferation of cultured type Ⅱ pneumocytes accompanied by thedownregulation of PCNA,and induces mitochondria-mediated apoptosis involv-ing the activation of p38-MAPK.

关键词： nitrofen pulmonary hypoplasia proliferating cell nuclear antigen apoptosis-inducing factor

Study on the hydrogen absorption properties of a YGdTbDyHo rare-earth high-entropy alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 127-135页

作者： tongyue Li Ziliang Xie Wenjiao Zhou Huan tong Dawen Yang Anjia Zhang Yuan Wu Xiping song State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China Whole Win(Beijing)Materials Sci.&Tech.Co. Ltd.Beijing 100083China

This study investigated the microstructure and hydrogen absorption properties of a rare-earth high-entropy alloy(HEA),*** indicated that the YGdTbDyHo alloy had a microstructure of equiaxed grains,with the alloy elements distributed *** hydrogen absorption,the phase structure of the HEA changed from a solid solution with an hexagonal-close-packed(HCP)structure to a high-entropy hydride with an faced-centered-cubic(FCC)structure without any secondary phase *** alloy demonstrated a maximum hydrogen storage capacity of 2.33 H/M(hydrogen atom/metal atom)at 723 K,with an enthalpy change(ΔH)of-141.09 kJ·mol^(-1)and an entropy change(ΔS)of-119.14 J·mol^(-1)·K^(-1).The kinetic mechanism of hydrogen absorption was hydride nucleation and growth,with an apparent activation energy(E_(a))of 20.90 kJ·mol^(-1).Without any activation,the YGdTbDyHo alloy could absorb hydrogen quickly(180 s at 923 K)with nearly no incubation period *** reason for the obtained value of 2.33 H/M was that the hydrogen atoms occupied both tetrahedral and octahedral *** results demonstrate the potential application of HEAs as a high-capacity hydrogen storage material with a large H/M ratio,which can be used in the deuterium storage field.

关键词： rare-earth high-entropy alloy hydrogen absorption capacity pressure–composition–temperature curves kinetics

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De novo synthesis of atropisomeric benzofurans via Cu/SPDO complex catalyzed asymmetric formal [3 + 2] annulation

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Science China Chemistry 2025年第1期68卷 174-180页

作者： Fan-Xiao Meng Xing-Yu Wang Ka Lu Ju-song Yang Fu-Min Zhang Ai-Jun Ma Bao Du Yong-qiang Tu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical Engineering Lanzhou University School of Chemistry and Chemical Engineering Shanghai Jiao Tong University School of Biotechnology and Health Sciences Wuyi University

Herein, the de novo synthesis of atropisomeric benzofurans through the Cu/spirocyclic-pyrrolidine-oxazoline(SPDO) complex catalyze asymmetric formal [3 + 2] cycloaddition between quinones and ynamides is explored, producing a range of structurally diverse axially chiral benzofurans in satisfactory yields with good to excellent enantioselectivities. Furthermore, density functional theory calculations were applied to explain the possible reaction mechanism.

关键词： benzofuran atropisomers novel de novo synthetic strategy asymmetric formal [3 + 2] cycladdition Cu/SPDO complex DFT calculations

Multilevel analysis of the central-peripheral-target organ pathway:contributing to recovery after peripheral nerve injury

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Neural Regeneration Research 2025年第10期20卷 2807-2822页

作者： Xizi song Ruixin Li Xiaolei Chu Qi Li Ruihua Li Qingwen Li Kai-Yu tong Xiaosong Gu Dong Ming Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Haihe Laboratory of Brain-Machine Interface and Human-Machine Fusion TianjinChina Department of Rehabilitation Tianjin University Tianjin HospitalTianjinChina Department of Hand Microsurgery Tianjin University Tianjin HospitalTianjinChina School of Exercise and Health Tianjin University of SportTianjinChina Department of Biomedical Engineering the Chinese University of Hong KongHong Kong Special Administrative RegionChina

Peripheral nerve injury is a common neurological condition that often leads to severe functional limitations and *** on the pathogenesis of peripheral nerve injury has focused on pathological changes at individual injury sites,neglecting multilevel pathological analysis of the overall nervous system and target *** has led to restrictions on current therapeutic *** this paper,we first summarize the potential mechanisms of peripheral nerve injury from a holistic perspective,covering the central nervous system,peripheral nervous system,and target *** peripheral nerve injury,the cortical plasticity of the brain is altered due to damage to and regeneration of peripheral nerves;changes such as neuronal apoptosis and axonal demyelination occur in the spinal *** nerve will undergo axonal regeneration,activation of Schwann cells,inflammatory response,and vascular system regeneration at the injury *** damage to target organs can occur,including skeletal muscle atrophy and sensory receptor *** then provide a brief review of the research advances in therapeutic approaches to peripheral nerve *** main current treatments are conducted passively and include physical factor rehabilitation,pharmacological treatments,cell-based therapies,and physical ***,most treatments only partially address the problem and cannot complete the systematic recovery of the entire central nervous system-peripheral nervous system-target organ ***,we should further explore multilevel treatment options that produce effective,long-lasting results,perhaps requiring a combination of passive(traditional)and active(novel)treatment methods to stimulate rehabilitation at the central-peripheral-target organ levels to achieve better functional recovery.

关键词： central nervous system central peripheral target organ multilevel pathological analysis nerve regeneration peripheral nerve injury peripheral nervous system target organs therapeutic approach

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