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Establishment of an efficient reverse genetic system of Mumps virus S79 from cloned DNA

World Journal of Pediatrics 2019年第5期15卷 499-505页

作者： Duo Zhou Meng-Ying Zhu Yi-Long Wang Xiao-qiang hao Dong-Ming Zhou Rong-Xian Liu Chu-Di Zhang Chu-Fan Qu Zheng-Yan Zhao Zhejiang University School of Medicine HangzhouChina Children's Hospital Zhejiang University School of MedicineHangzhou 310052China Department of Neurology Children's HospitalZhejiang University School of MedicineHangzhou 310052China

Background Mumps is a common type of respiratory infectious disease caused by mumps virus(MuV),and can be effectively prevented by *** this study,a reverse genetic system of MuV that can facilitate the rational design of safer,more efficient mumps vaccine candidates is *** MuV-S79 cDNA clone was assembled into a full-length plasmid by means of the GeneArtTM High-Order Genetic Assembly System,and was rescued via reverse genetic ***-PCR,sequencing,and immunofluorescence assays were used for rMuV-S79 *** replication kinetics and in vivo experimental models were used to evaluate the replication,safety,and immunogenicity of *** A full-length cDNA clone of MuV-S79 in the assembly process was generated by a novel plasmid assemble strategy,and a robust reverse genetic system of MuV-S79 was successfully *** established rMuV-S79 strain could reach a high virus titer in *** average viral titer of rMuV-S79 in the lung tissues was 2.68±0.14 log10PFU/g lung tissue,and rMuV-S79 group did not induce inflammation in the lung tissues in cotton *** antibody titers induced by rMuV-S79 were high,long-lasting and could provide complete protection against MuV wild strain *** We have established a robust reverse genetic system of MuV-S79 which can facilitate the optimization of mumps ***-S79 rescued could reach a high virus titer and the safety was proven in *** could also provide complete protection against MuV wild strain challenge.

关键词： Mumps virus Reverse genetics Plasmid Safety Immunogenicity

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Metabolomic-based analysis reveals bile acid-mediated ovarian failure induced by low temperature in zebrafish

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Zoological Research 2024年第4期45卷 791-804页

作者： Wen-hao Li Zhi-qiang Li Meng-Di Bu Jia-Zhen Li Liang-Biao Chen Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationCollege of Fisheries and Life SciencesShanghai Ocean UniversityShanghai 201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdaoShandong 266237China International Research Center for Marine Biosciences Ministry of Science and TechnologyCollege of Fisheries and Life SciencesShanghai Ocean UniversityShanghai 201306China

As ectotherms, fish are highly sensitive to temperature fluctuations, which can profoundly impact their reproductive cycles. In this study, we investigated the fertility and histological characteristics of zebrafish(Danio rerio) ovaries exposed to a temperature gradient ranging from the thermopreferendum temperature of the species,27℃, to lower temperatures of 22℃, 20℃, and 13℃ over a period of two weeks. Comparative metabolomic(six biological replicates for each temperature) and transcriptomic(four biological replicates for each temperature) analyses were conducted under the four temperature conditions. Results indicated that lower temperatures inhibited oocyte development and differential metabolites were involved in steroid hormone production,antioxidant function, and lipid and protein *** reproductive hormones, increased proteolysis,and lipid degradation significantly impeded oocyte development and egg maturation. Notably, a significant increase in bile acid content was noted in the ovaries of the cold-treated fish, indicating that bile acids play a critical role in ovarian failure. Overall, these findings provide valuable insights into the mechanisms governing the reproductive response of fish to cold stress.

关键词： old stress Ovarian failure Metabolome Zebrafish

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Multi-Objective Optimization Algorithm for Grouping Decision Variables Based on Extreme Point Pareto Frontier

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Computers, Materials & Continua 2024年第4期79卷 1281-1299页

作者： JunWang Linxi Zhang hao Zhang Funan Peng Mohammed A.El-Meligy Mohamed Sharaf qiang Fu College of Computer Science and Technology Shenyang University of Chemical TechnologyShenyang110142China Key Laboratory of Intelligent Technology for Chemical Process Industry of Liaoning Province Shenyang University of Chemical TechnologyShenyang110142China Industrial Engineering Department College of EngineeringKing Saud UniversityP.O.Box 800Riyadh11421Saudi Arabia

The existing algorithms for solving multi-objective optimization problems fall into three main categories:Decomposition-based,dominance-based,and *** multi-objective optimization problemsmainly focus on objectives,treating decision variables as a total variable to solve the problem without consideringthe critical role of decision variables in objective *** seen,a variety of decision variable groupingalgorithms have been ***,these algorithms are relatively broad for the changes of most decisionvariables in the evolution process and are time-consuming in the process of finding the Pareto *** solvethese problems,a multi-objective optimization algorithm for grouping decision variables based on extreme pointPareto frontier(MOEA-DV/EPF)is *** algorithm adopts a preprocessing rule to solve the Paretooptimal solution set of extreme points generated by simultaneous evolution in various target directions,obtainsthe basic Pareto front surface to determine the convergence effect,and analyzes the convergence and distributioneffects of decision *** the later stages of algorithm optimization,different mutation strategies are adoptedaccording to the nature of the decision variables to speed up the rate of evolution to obtain excellent individuals,thusenhancing the performance of the *** validation of the test functions shows that this algorithmcan solve the multi-objective optimization problem more efficiently.

关键词： Multi-objective evolutionary optimization algorithm decision variables grouping extreme point pareto frontier

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A compact X-band backward traveling-wave accelerating structure

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Nuclear Science and Techniques 2024年第5期35卷 13-29页

作者： Xian-Cai Lin hao Zha Jia-Ru Shi qiang Gao Fang-Jun Hu Qing-Zhu Li Huai-Bi Chen Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Particle and Radiation Imaging(Tsinghua University) Ministry of EducationBeijing 100084China

Very high-energy electrons(VHEEs)are potential candidates for FLASH radiotherapy for deep-seated *** proposed a compact VHEE facility based on an X-band high-gradient high-power *** this study,we investigated and realized the first X-band backward traveling-wave(BTW)accelerating structure as the buncher for a VHEE facility.A method for calculating the parameters of single cell from the field distribution was introduced to simplify the design of the BTW ***-domain circuit equations were applied to calculate the transient beam parameters of the buncher in the unsteady state.A prototype of the BTW structure with a thermionic cathode-diode electron gun was designed,fabricated,and tested at high power at the Tsinghua X-band high-power test *** structure successfully operated with 5-MW microwave pulses from the pulse compressor and outputted electron bunches with an energy of 8 MeV and a pulsed current of 108 mA.

关键词： Backward traveling-wave accelerating structure Equivalent circuit model High-power test Very high-energy electron radiotherapy

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Effect of short-time low-temperature austenitizing on microstructure and mechanical properties of DT300 ultra-high strength steel fabricated by laser powder bed fusion

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Journal of Iron and Steel Research International 2024年第7期31卷 1763-1777页

作者： Chen-yang Jiang Xiao-qiang Li Jin-tao Wang hao Luo Sheng-qing Gao Sheng-guan Qu Chao Yang National Engineering Research Centre of Near-Net-Shape Forming for Metallic Materials South China University of TechnologyGuangzhou 510640GuangdongChina Institute of New Materials Guangdong Academy of ScienceGuangzhou 510650GuangdongChina

To address the inhomogeneous microstructure and improve the mechanical properties of DT300 ultra-high strength steel specimens fabricated by laser powder bed fusion,different post-heat treatment schedules are *** the increase in austenitizing temperature and time,the migration rate of austenite grain boundaries continuously increases with the dissolution of nano-carbides,and the formation of nano-oxides and twin martensite is also inhibited *** rapid growth in the size of prior austenite grains and martensite laths,as well as the decrease in the content of nano-oxides and twin martensite,led to a rapid decrease in the strength(yield strength and ultimate tensile strength)from HT2 to HTF *** HT1 specimens(austenitizing at 830℃for 30 min,then oil quenching and tempering at 300℃for 120 min and finally air cooling)display excellent mechanical properties of yield strength of 1572 MPa,ultimate tensile strength of 1847 MPa,elongation of 9.84%,and fracture toughness of 106 MPa m^(1/2),which are counterparts to those of conventional DT300 steel forgings after heat treatment.

关键词： DT300 ultra-high strength steel Laser powder bed fusion-Short-time low-temperature austenitizing Microstructure Mechanical property

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Efficient second and third harmonic generation in dual-layer lithium niobate microdisk resonator

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Science China(Physics,Mechanics & Astronomy) 2024年第5期67卷 33-38页

作者： Zhenzhong hao Xiao Wu qiang Luo Zhang Li Rui Ma Fang Bo Feng Gao Guoquan Zhang Jingjun Xu MOE Key Laboratory of Weak-Light Nonlinear Photonics TEDA Institute of Applied Physics and School of PhysicsNankai UniversityTianjin 300457China

Lithium niobate thin film frequency doubler has extensive applications in the preparation of classical and quantum *** this study,we successfully fabricated microdisk resonators with a quality factor of 2.2×10^(5) in reverse-polarization dual-layer x-cut lithium niobate for the first *** on the modal phase matching condition,efficient second harmonic generation with a record normalized conversion efficiency of~56000%W-1 and cascaded third harmonic generation with an efficiency of~6500%W-2 were obtained in the microdisk *** with the periodically poled lithium niobate microcavity,the complex domain structure preparation processes are *** work provides a scheme for achieving highly efficient second-order nonlinear effects in non-periodically poled microcavities.

关键词： harmonic lithium niobate

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Reinforced SnO_(2) tensile-strength and“buffer-spring”interfaces for efficient inorganic perovskite solar cells

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Carbon Energy 2024年第6期6卷 282-291页

作者： Yuanyuan Zhao Lei Gao Qiurui Wang qiang Zhang Xiya Yang Jingwei Zhu hao Huang Jialong Duan Qunwei Tang College of Mechanical and Electronic Engineering Shandong University of Science and TechnologyQingdaoChina Institute of New Energy Technology College of Information Science and TechnologyJinan UniversityGuangzhouChina College of Materials Science and Engineering Sichuan UniversityChengduChina Guangxi Key Laboratory of Processing for Non-Ferrous Metals and Featured Materials School of ResourcesEnvironment and MaterialsGuangxi UniversityNanningChina Institute of Carbon Neutrality College of Chemical and Biological EngineeringShandong University of Science and TechnologyQingdaoChina

Suppressing nonradiative recombination and releasing residual strain areprerequisites to improving the efficiency and stability of perovskite solar cells(PSCs).Here,long-chain polyacrylic acid(PAA)is used to reinforce SnO_(2)film and passivate SnO_(2)defects,forming a structure similar to“reinforcedconcrete”with high tensile strength and fewer ***,PAA is also introduced to the SnO_(2)/perovskite interface as a“buffer spring”torelease residual strain,which also acts as a“dual-side passivation interlayer”to passivate the oxygen vacancies of SnO_(2)and Pb dangling bonds in *** a result,the best inorganic CsPbBr_(3)PSC achieves a championpower conversion efficiency of 10.83%with an ultrahigh open-circuit voltageof 1.674 *** unencapsulated PSC shows excellent stability under 80%relative humidity and 80℃over 120 days.

关键词： charge recombination defect passivation inorganic perovskite solar cells interfacial modification strain relaxation

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A　Conformational　Study　on　the　Interaction　of　Saporin-S6　with　Phospholipid　Vesicles

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Chemical Research in Chinese Universities 1998年第1期14卷 42-45页

作者： hao, qiang Guan, Zhong-hui Yan, Li Zhang, Yan Yan, Gang-lin Liu, Guo-hong Dong, Qing-chu Li, Qing-shan Wang, Yan-zhong Jilin Univ Dept Mol Biol Changchun 130023 Peoples R China Jilin Univ Natl Lab Enzyme Engn Changchun 130023 Peoples R China Peoples Hosp Jilin Prov Med Testing Dept Changchun 130021 Peoples R China

ＡＣｏｎｆｏｒｍａｔｉｏｎａｌＳｔｕｄｙｏｎｔｈｅＩｎｔｅｒａｃｔｉｏｎｏｆＳａｐｏｒｉｎ－Ｓ６ｗｉｔｈＰｈｏｓｐｈｏｌｉｐｉｄＶｅｓｉｃｌｅｓＨＡＯＱｉａｎｇ，ＧＵＡＮＺｈｏｎｇ－ｈｕｉ，ＹＡＮＬｉ，ＺＨＡＮＧＹａ... 详细信息

ＡＣｏｎｆｏｒｍａｔｉｏｎａｌＳｔｕｄｙｏｎｔｈｅＩｎｔｅｒａｃｔｉｏｎｏｆＳａｐｏｒｉｎ－Ｓ６ｗｉｔｈＰｈｏｓｐｈｏｌｉｐｉｄＶｅｓｉｃｌｅｓＨＡＯＱｉａｎｇ，ＧＵＡＮＺｈｏｎｇ－ｈｕｉ，ＹＡＮＬｉ，ＺＨＡＮＧＹａｎ，ＹＡＮＧａｎｇ－ｌｉｎ，ＬＩＵ...

关键词： Interaction the Phospholipid Saporin-S6

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Selective photocatalytic oxidation of methane to C1 oxygenates by regulating sizes and facets over Au/ZnO

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Nano Research 2024年第5期17卷 3810-3818页

作者： qiang Zhou Xinyu Wang Xiaojie Tan Qinhua Zhang hao Yang Tao Xing Mingqing Wang Mingbo Wu Wenting Wu State Key Laboratory of Heavy Oil Processing College of Chemistry and Chemical Engineering&Institute of New EnergyChina University of Petroleum(East China)Qingdao 266580China National Engineering Research Center of Coal Gasification and Coal-Based Advanced Materials ShanDong Energy Group CO.LTDJinan 250101China

Photocatalytic oxidation of methane to value-added chemicals is a promising process under mild conditions,nevertheless confronting great challenges in efficiently activating C-H bonds and inhibiting ***,we propose a comprehensive strategy for the selective generation of reactive oxygen species(ROS)by regulating the sizes and facets of Au nanoparticles loaded on *** photocatalytic methane oxidation at ambient temperature,a high oxygenates yield of 36.4 mmol·g^(-1)·h^(-1) with a nearly 100%selectivity has been achieved over the optimized 1.0%Au/ZnO-9.6(1%Au with(111)facet and 9.6 nm size on ZnO)photocatalyst,exceeding most reported *** investigations reveal that 1.0%Au/ZnO-9.6 with the medium size and Au(111)facet guarantees the favourable formation of superoxide radicals(·OOH)through mild oxygen reduction,ultimately leading to excellent photocatalytic methane oxidation *** work provides some guidance for the delicate design of photocatalysts for efficient photocatalytic methane oxidation and oxygen utilization.

关键词： photocatalysis methane oxidation radical regulation oxygen activation nanomaterial

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Panax Notoginseng Saponins Inhibits Ventricular Remodeling after Myocardial Infarction in Rats Through Regulating ATF3/MAP2K3/p38 MAPK and NFκB Pathway

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Chinese Journal of Integrative Medicine 2020年第12期26卷 897-904页

作者： MA Ru-feng CHEN Guang LI Hong-zheng ZHANG Yun LIU Yong-mei HE hao-qiang LIU Chen-yue XIE Zi-cong ZHANG Zhen-peng WANG Jie Graduate School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing(100029)China Department of Cardiology Guang’anmen HospitalChina Academy of Chinese Medical SciencesBeijing(100053)China

Objective:To explore whether Panax notoginseng saponins(PNS)exhibits heart protective effect in myocardial infarction(MI)rats and to identify the potential signaling pathways ***:MI rats induced by ligating the left anterior descending(LAD)coronary artery were assigned to sham coronary artery ligation or coronary artery *** 36 Sprague-Dawley rats were randomly divided into sham group(distilled water,n=9),MI group(distilled water,n=9),PNS group(PNS,40 mg/kg daily,n=9)and fosinopril group(FIP,1.2 mg/kg daily,n=9)according to a random number *** left ventricular morphology and function were conducted by *** alterations were evaluated by the stainings of HE and *** serum levels of C reactive protein(CRP),tumor necrosis factorα(TNF-α),growth differentiation factor-15(GDF-15)and the ratio of metalloproteinase-9(MMP-9)and tissue inhibitor of MMP-9(TIMP-1)were determined by *** levels of activating transcription factor 3(ATF3),mitogen-activated protein kinase kinase 3(MAP2 K3),p38 mitogen-activated protein kinase(p38 MAPK),phosphorylation of p38 MAPK(p-p38 MAPK),transforming growth factor-β(TGF-β1),collagenⅠ,nuclear factor kappa B p65(NFκB p65),phosphorylation of NFκB p65(p-NFκB p65),and phosphorylation of inhibitory kappa Bα(p-IκBα)in hearts were measured by Western blot and immunohistochemical staining,***:PNS improved cardiac function and fibrosis in MI rats(P<0.05).The serum levels of CRP,TNF-α,GDF-15 and the ratio of MMP9/TIMP1 were reversed by PNS in MI *** expressions of TGF-β1,collagenⅠ,MAP2 K3,p38 MAPK,p-p38 MAPK,NFκB p65,p-NFκB p65,and p-IκBαwere down-regulated,while ATF3 increased with the treatment of PNS(P<0.05).Conclusions:PNS may improve cardiac function and fibrosis in MI rats via regulating ATF3/MAP2 K3/p38 MAPK and NFκB signaling *** results suggest the potential of PNS in preventing the development of ventricular remodeling in MI rats.

关键词： Panax notoginseng saponins myocardial infarction ventricular remodeling inflammation

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