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Consecutive Monitoring of Interleukin-6 Is Needed for COVID-19 Patients

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Virologica Sinica 2021年第5期36卷 1093-1096页

作者： Xiaohua Chen Juan Zhou Chen Chen Baidong Hou Ashaq Ali Feng Li Zhaolin Hua Yingtao Wu Qin Yang Min Chen Rong Zhang qianchuan huang Jinya Ding Xian-En Zhang Dong Men Department of Laboratory Medicine General Hospital of Central Theater CommandPLAWuhan 430070China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Department of Laboratory Medicine General Hospital of Southern Theater CommandPLAGuangzhou 510000China State Key Laboratory of Virology School of Basic Medical SciencesWuhan UniversityWuhan 430071China Hubei Provincial Key Laboratory of Allergy and Immunology Wuhan 430071China University of Chinese Academy of Sciences Beijing 100039China

Dear Editor,The world is facing an immense challenge in the fight against coronavirus disease 2019(COVID-19),which is caused by severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)(Gorbalenya et al.2020).To date,tens of millions of people worldwide have been infected with SARS-Co V-2,and more than a million have died,and the situation continues.

关键词： Interleukin al. acute

RNAi抑制Bmi-1基因表达对Jurkat细胞的影响(英文)

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The Chinese-German Journal of Clinical Oncology 2012年第12期11卷 732-736页

作者： Shangen Zheng Qibin Jing Yaqiong Zheng Yinjuan Ding qianchuan huang Guoqiang Zhao Department of Clinical Transfusion Wuhan General Hospital of Guangzhou Millitary CommandWuhan 430070China Health Care Agency of Air Force Early Institute Wuhan 430019China Department of Microbiology Basic Medical Science SchoolZhengzhou UniversityZhengzhou 450000China

Objective: The aim of our study was to investigate the effects of down-regulation Bmi-1 by RNA interference (RNAi) in T Lymphocytic leukemia Jurkat cells. Methods: Two complementary oligonucleotide strands were synthesized based on the siRNA sequence targeting Bmi-1 gene. After annealing, siRNA strands were recombined into the pRNAT-U6.2 vector, and then DNA sequencing was carried out following transformation and amplification. The recombinant was transfected into Jurkat cells with liposomes. Positive colonies were obtained through G418 selection. The mRNA and protein expressions of Bmi-1 were detected by RT-PCR and Western-blot, respectively. Effects of Bmi-1 silence on cell proliferation, cell cycle and cell aging of Jurkat cells were detected by MTT assay, flow cytometry, colony formation assay and SA-β-Gal staining, respectively. Results: The siRNA recombinant targeting Bmi-1 gene was successfully constructed. All three siRNA recombinants could significantly inhibit the expression of Bmi-1. The siRNA targeting 825nt-843nt (GACCAGACCACTACT GAAT) has the strongest inhibitory effect on Bmi-1 expression, with almost complete inhibition on Bmi-1 mRNA and protein expressions. Compared with the non-transfection group and the empty vector group, growth velocity and colony formation ability were significantly decreased, while the proportion of cells in G1 phase and the percentage of senile cells were signifi-cantly increased in highly transfected group (P < 0.05). Conclusion: Down-regulation Bmi-1 by RNA interference (RNAi) could significantly inhibit the growth of Jurkat cells in vitro.

关键词： Jurkat细胞 RNA干扰 Western-blot siRNA 脂质体转染淋巴细胞白血病 RT-PCR RNAi

过表达人血管生成素样蛋白4对食管癌EC9706细胞生长的影响(英文)

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The Chinese-German Journal of Clinical Oncology 2010年第2期9卷 101-105页

作者： Shangen Zheng Fang Wang Yinjuan Ding Lifang Zhang qianchuan huang Guoqiang Zhao Department of Transfusion Wuhan General Hospital of Guangzhou Millitary Command Wuhan 430070 China Health Department Air Force Radar Academy Wuhan 430019 China Department of Microbiology Basic Medical Science School Zhengzhou University Zhengzhou 450000 China

Objective:The aim of the study was to construct the eukaryotic expression vector of human angiopoietin-like protein 4(ANGPTL4) and observe the effect of ANGPTL4 overexpression on the growth of esophageal carcinoma EC9706 cells.Methods:Total RNA was extracted from normal hepatic tissue,and ANGPTL4 cDNA was amplified by RT-PCR.The PCR product was doubly digested by XbaI and SalI,and then recombined into eukaryotic expression vector.Then,pIRES-GFP-ANGPTL4 was obtained by G418 selection,then pIRES-GFP-ANGPTL4 and pIRES-GFP were transfected into EC9706 cells with lipidosome-packaged method.Meanwhile,the transfected cells were selected by G418,and then stable transfected cell lines were obtained.ANGPTL4 mRNA levels,the cell cycles and growth curves of EC9706 cells in experiment group(transfected with pIRES-GFP-ANGPTL4),empty vector group(transfected with pIRES-GFP) and blank control group(EC9706 cells without transfection) were detected with RT-PCR,flow cytometry and MTT methods,respectively.Results:Eukaryotic ANGPTL4 expression vector pIRES-GFP-ANGPTL4 was successfully constructed.The ANGPTL4 mRNA level(0.21 ± 0.03) in experiment group was significantly higher than that of the empty vector group(0.04 ± 0.008) and the blank control group(0.05 ± 0.007),with significant differences(P < 0.01).The proportion of cells in S phase in experiment group was significantly different with those of the other two groups(P < 0.05).The cell growth of EC9706 cells in experiment group was slower than those of the other two groups.From the third day,the differences began to be significant.Conclusion:ANGPTL4 overexpression in esophageal carcinoma EC9706 cells could inhibit the growth of EC9706 cells.

关键词：血管生成素过度表达细胞生长食管癌蛋白

Correction:Lymphopenia predicts disease severity of COVID-19:a descriptive and predictive study

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1952-1952页

作者： Li Tan Qi Wang Duanyang Zhang Jinya Ding qianchuan huang Yi-Quan Tang Qiongshu Wang d Hongming Miao Department of Disease Control and Prevention General Hospital of Central Theater Command 430015 Wuhan Hubei province People's Republic of China. Department of Laboratory General Hospital of Central Theater Command 430015 Wuhan Hubei province People's Republic of China. MRC Laboratory of Molecular Biology Cambridge Biomedical Campus Cambridge CB2 0QH UK. 4Department of Biochemistry and Molecular Biology Third Military Medical University (Army Medical University) 400038 Chongqing People's Republic of China.

Correction to:Signal Transduction and Targeted Therapy https://***/10.1038/s41392-020-0148-4,published online 27 March 2020 Since the publication of this article,1 we noticed a minor mistake in the reference that need... 详细信息

关键词： severity Lymph Therapy

ACE2在SARS-CoV-2靶器官损伤中作用的研究进展

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国际呼吸杂志 2023年第5期43卷 516-520页

作者：刘莹刁波黄前川中部战区总医院检验科武汉430070 中部战区总医院基础医学实验科武汉430070 中部战区总医院中枢神经系统肿瘤的发生与干预湖北省重点实验室武汉430070

血管紧张素转换酶2(ACE2)在体内广泛分布, 是肾素-血管紧张素系统重要调控因子, 对维持人体血压和内环境稳定至关重要;同时, ACE2还是严重急性呼吸综合征冠状病毒2的受体, 介导病毒进入宿主细胞而引起的COVID-19, COVID-19可以表现为支... 详细信息

血管紧张素转换酶2(ACE2)在体内广泛分布, 是肾素-血管紧张素系统重要调控因子, 对维持人体血压和内环境稳定至关重要;同时, ACE2还是严重急性呼吸综合征冠状病毒2的受体, 介导病毒进入宿主细胞而引起的COVID-19, COVID-19可以表现为支气管炎、肺炎或严重的呼吸道疾病, 还可伴有肺、心、肾等多个器官的损伤。本文就ACE2在COVID-19中对靶器官的损伤作用进行总结, 为临床上保护COVID-19患者器官功能, 及抗病毒药物的研发提供参考。

关键词：新型冠状病毒感染新型冠状病毒血管紧张素转换酶2 肾素-血管紧张素系统多器官损伤

一种基于径向基函数神经网络的电子侦察无人机资源调度方法

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航天电子对抗 2023年第4期39卷 11-14,19页

作者：黄黔川刘盼盼电子信息控制重点实验室四川成都610036

针对电子侦察无人机的资源调度问题,将电子侦察资源调度问题转换成目标优先级分配问题,并提出了一种基于径向基函数神经网络的电子侦察资源调度方法,通过对目标分配优先级进行智能学习分配,达到侦察资源利用率最大化。仿真结果表明,该... 详细信息

针对电子侦察无人机的资源调度问题,将电子侦察资源调度问题转换成目标优先级分配问题,并提出了一种基于径向基函数神经网络的电子侦察资源调度方法,通过对目标分配优先级进行智能学习分配,达到侦察资源利用率最大化。仿真结果表明,该方法可以根据侦察目标的多维度信息自适应分配无人机侦察资源,相比传统传感器资源分配调度更加高效。

关键词：电子侦察无人机径向基函数神经网络资源调度

乳腺癌组织中DNA修复蛋白Ku80的表达及其生物学意义

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标记免疫分析与临床 2022年第4期29卷 677-681页

作者：胡李珩黄前川洪伟湖北省咸宁市通城县人民医院湖北咸宁437400 武汉中核中同蓝博医学检验实验室湖北武汉430000

目的探讨乳腺癌组织中Ku80蛋白的表达及其生物学意义。方法选取武汉中核中核中同蓝博医学检验实验室2010年1月至2019年12月确诊的80例原发性乳腺癌患者的手术切除标本,采用免疫组织化学分析原发性乳腺癌组织Ku80的表达与细胞增殖相关活... 详细信息

目的探讨乳腺癌组织中Ku80蛋白的表达及其生物学意义。方法选取武汉中核中核中同蓝博医学检验实验室2010年1月至2019年12月确诊的80例原发性乳腺癌患者的手术切除标本,采用免疫组织化学分析原发性乳腺癌组织Ku80的表达与细胞增殖相关活性蛋白(Ki67)、基质金属蛋白酶2(MMP-2)、人表皮生长因子受体2(HER2)、P53的相关性;培养人乳腺癌细胞BT-474,分为siRNA-Ku80组、siRNA-阴性对照组和空白对照组,应用Western Blot、Transwell法和MTT法分别检测各组Ki67、MMP-2、P53和HER2水平、细胞侵袭能力和增殖情况。结果乳腺癌组织中Ku80与HER-2、Ki67和MMP-2的表达呈正相关(P0.05);Western Blot结果显示siRNA-Ku80组乳腺癌细胞HER2,Ki67和MMP-2蛋白的表达下降,与对照组和空白对照组差异有统计学意义(P0.05);Transwell结果显示siRNA-Ku80组侵袭细胞计数明显减少,与对照组和空白对照组差异有统计学意义(P<0.05);MTT结果显示siRNA-Ku80对乳腺癌细胞BT-474生长有抑制作用,在24h、48h和72h的抑制率分别为9.83%、39.71%和51.20%,与空白组和siRNA对照组比较差异有统计学意义(P<0.05)。结论乳腺癌组织中Ku80的表达与HER-2、Ki67和MMP-2的表达呈正相关;RNA干扰抑制Ku80后,乳腺癌细胞HER-2、Ki67和MMP-2的表达减少,乳腺癌细胞的增殖力和侵袭力降低;提示Ku80的异常表达可能与乳腺癌发生相关。

关键词： Ku80 乳腺癌细胞增殖侵袭力转移

单核-巨噬细胞在新冠肺炎患者炎症反应中的作用初探

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标记免疫分析与临床 2020年第7期27卷 1266-1269页

作者：洪伟黄前川北京中同蓝博医学检验实验室北京100070 中部战区总医院检验科湖北武汉430070

由SARS-CoV-2感染引起的COVID-19大流行已导致全世界54万多人死亡。多项研究证实,超级炎症反应是COVID-19严重程度和死亡的主要原因。单核-巨噬细胞是固有免疫细胞群,通过吞噬或释放炎性分子清除病原体并促进组织的修复,然而,单核-巨噬... 详细信息

由SARS-CoV-2感染引起的COVID-19大流行已导致全世界54万多人死亡。多项研究证实,超级炎症反应是COVID-19严重程度和死亡的主要原因。单核-巨噬细胞是固有免疫细胞群,通过吞噬或释放炎性分子清除病原体并促进组织的修复,然而,单核-巨噬细胞功能过度激活形成的超级炎症,却可导致COVID-19患者器官损害甚至死亡,因此,本文对单核-巨噬细胞在SARS-CoV-2感染中的潜在病理作用进行综述,并探讨了调控巨噬细胞活化在COVID-19中的治疗策略。

关键词：新冠肺炎单核-巨噬细胞超级炎症凝血异常治疗

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利用主客观集成赋权法的多目标威胁评估

电讯技术 2019年第8期59卷 956-961页

作者：侯思尧李永光陈思静黄黔川中国电子科技集团公司第二十九研究所

在利用逼近于理想解的排序法(Technique for Order Preference by Similarity to an Ideal Solution,TOPSIS)进行多目标威胁评估时,针对如何获取合理的目标威胁评估因子的权重比,提出了一种主客观集成赋权方法。该方法首先利用主观赋权... 详细信息

在利用逼近于理想解的排序法(Technique for Order Preference by Similarity to an Ideal Solution,TOPSIS)进行多目标威胁评估时,针对如何获取合理的目标威胁评估因子的权重比,提出了一种主客观集成赋权方法。该方法首先利用主观赋权法和客观赋权法获取两组权重值;然后,通过构造多目标规划模型,将两组权重值进行综合处理,得到更合理的权重值。仿真结果表明,相较于主观赋权法和客观赋权法,所提方法计算出的威胁评估因子的权重值,在用TOPSIS法计算目标威胁评估时,能够得到更加合理、有效的评估结果。

关键词：电子对抗多目标威胁评估权重比主客观集成赋权法