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GENE TRANSFER INTO PORCINE MYOCARDIUM VIA PERICARDIAL CAVITY BY HOMEMADE EASY PUNCTURE DEVICE

Chinese Medical Sciences Journal 2007年第2期22卷 119-122页

作者： Huai-min Guan peng Liu Jin-hong Xie Feng-ling Wang Lin-sheng Cao Qi-jun qian Department of Cardiology Union Hospital Affiliated to Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Department of Cardiology Henan Provincial Chest Hospital Zhengzhou 450008 Department of Gene Therapy Oriental Hepatobiliary Hospital the Second Military Medical University Shanghai 200433

Objective To explore the feasibility and safety of gene transfer into porcine myocardium via the pericardial cavity by a homemade easy device. Methods Replication-deficient recombinant adenoviral vector carrying LacZ report gene (Ad-LacZ) was constructed by the calcium phosphate precipitation method. Twelve healthy Chinese mini-swine were randomly divided into experimental group (n=6) and control group (n=6). Acute myocardial infarction (AMI) model was established by balloon occlusion of the distal part of D1 branch of left anterior descending (LAD) artery, at the same time the intra-pericardial cavity injections were performed through the small incision of the abdominal wall below the xyphoid appendix using a homemade device. Then gene transfer was performed using a central venous catheter. The pericardium was pretreated with injection of a mixture of collagenase (1 200 U) and hyaluronidase (3 000 U) in both groups. Then 2.0×109 plaque formation unit (PFU) Ad-LacZ was injected into the pericardial cavity in experimental group, while 1 mL of normal saline was injected in the control group. The β-galactosidase activity detection and X-gal staining of the ischemic myocardium were performed on the 3rd, 7th, and 28th day after injection. Results The LAD artery was occluded completely and infarction and ischemia were detected by histological assessment. In experimental group, the X-gal staining positive cells and β-galactosidase activity quantification were detectable on the 3rd day after injection, increased markedly on the 7th day, and then declined on the 28th day. The transfer efficiencies indicated by the positive myocardial cells were 16.7%, 45.6%, 22.8% on the 3rd, 7th, 28th day, respectively. In control group, no positive cells and β-galactosidase activity were observed. Conclusion Adenovirus can be transferred into ischemic myocardium and express target gene in the AMI model for four weeks with the homemade easy device via pericardial cavity pretreated by collag

关键词： adenovirus acute myocardial infarction model gene transfer pericardial cavity

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Assessment of iris volume in glaucoma patients with type 2 diabetes mellitus by AS-OCT

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International Journal of Ophthalmology(English edition) 2023年第5期16卷 743-747页

作者： Ying Su Qing-Shu Ge Zong-Yi Li qian-wen Bu Die Hu Long-Fang Zhou Xiao-Jing Pan School of Clinical Medicine Weifang Medical UniversityWeifang 261021Shandong ProvinceChina Eye Institute of Shandong First Medical University Qingdao Eye Hospital of Shandong First Medical UniversityQingdao 266071Shandong ProvinceChina State Key Laboratory Cultivation Base Shandong Provincial Key Laboratory of OphthalmologyQingdao 266071Shandong ProvinceChina School of Ophthalmology Shandong First Medical UniversityQingdao 266071Shandong ProvinceChina

AIM:To examine the change of iris volume measured by CASIA2 anterior segment optical coherence tomography(ASOCT)in glaucoma patients with or without type 2 diabetes mellitus(T2DM)and explore if there is a correlation between hemoglobin A1c(HbA1c)level and iris ***:In a cross-sectional study,72 patients(115 eyes)were divided into two groups:primary open angle glaucoma(POAG)group(55 eyes)and primary angle-closure glaucoma(PACG)group(60 eyes).Patients in each group were separately classified into patients with or without *** volume and glycosylated HbA1c level were measured and ***:In the PACG group,diabetic patients'iris volume was significantly lower than those of non-diabetics(P=0.02),and there was a significant correlation between iris volume and HbA1c level in the PACG group(r=-0.26,P=0.04).However,diabetic POAG patients'iris volume was noticeably higher than those of non-diabetics(P=0.01),and there was a significant correlation between HbA1c level and iris volume(r=0.32,P=0.02).CONCLUSION:Diabetes mellitus impact iris volume size,as seen by increased iris volume in the POAG group and decreased iris volume in the PACG *** addition,iris volume is significantly correlated with HbA1c level in glaucoma *** findings imply that T2DM may compromise iris ultrastructure in glaucoma patients.

关键词： glaucoma diabetes mellitus iris volume glycosylated hemoglobin optical coherence tomography

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猪胞内劳森菌抗体间接ELISA检测方法的建立及应用

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中国预防兽医学报 2024年第1期46卷 55-60,69页

作者：裴艳艳张梦琳许浒相丽润张洪亮彭金美王倩田志军周国辉中国农业科学院哈尔滨兽医研究所动物疫病防控全国重点实验室黑龙江哈尔滨150069

猪增生性肠病(PPE),通常被称为猪回肠炎(PI),由胞内劳森氏菌(LI)感染引起。为建立检测LI抗体的间接ELISA方法,本研究构建LI外膜蛋白基因(LI0841)的重组表达质粒p ET28a-LI0841,经测序无误后转化BL21(DE3)宿主菌,经IPTG诱导表达,采用SDS-... 详细信息

猪增生性肠病(PPE),通常被称为猪回肠炎(PI),由胞内劳森氏菌(LI)感染引起。为建立检测LI抗体的间接ELISA方法,本研究构建LI外膜蛋白基因(LI0841)的重组表达质粒p ET28a-LI0841,经测序无误后转化BL21(DE3)宿主菌,经IPTG诱导表达,采用SDS-PAGE检测重组蛋白LI0841蛋白(rLI0841)的表达形式,经Ni柱纯化后采用western blot鉴定其反应原性。SDS-PAGE结果显示,在32 ku处出现目的条带,且其主要以可溶性形式表达;western blot结果显示,r LI0841能够与兔LI多克隆抗体特异性反应,表明纯化的r LI0841具有较强的反应原性,可作为包被抗原用于建立间接ELISA检测方法,经各反应条件优化初步建立LI抗体的间接ELISA检测方法。各反应条件的优化结果显示,4.38 ng/孔的r LI0841以4℃过夜包被最佳;血清最佳稀释度为1∶100,37℃反应0.5 h;羊抗猪Ig G-HRP最佳稀释度为1∶10000,37℃作用0.5 h;TMB底物37℃显色15 min。利用建立的间接ELISA方法检测猪繁殖与呼吸障碍综合征病毒、猪伪狂犬病病毒、副猪嗜血杆菌、猪链球菌、传染性胸膜肺炎放线杆菌及经美国Biostone PPE抗体检测试剂盒检测为阳性的猪血清,评估该方法的特异性;将LI阳性血清2倍倍比稀释(1∶100~1∶51200)后,采用本研究建立的间接ELISA方法检测,评估该方法的敏感性;以同一批次和不同批次包被的酶标板分别检测5份不同LI抗体水平的猪血清,评估该方法的重复性。结果显示,该方法除能检测到LI阳性血清外,其余相关病原的阳性血清均为阴性,特异性较强;LI阳性血清1∶800稀释时检测结果仍为阳性,敏感性较高;对5份不同抗体水平的LI阳性血清的批内、批间重复性试验的变异系数均小于10%,重复性较好。利用该ELISA方法与美国Biostone公司PPE抗体检测试剂盒同时检测104份临床猪血清样品,比较二者的检测结果,并计算二者的符合率;采用建立的间接ELISA方法检测黑龙江、吉林等地区413份临床猪血清样品,分析LI在上述地区的流行状况。结果显示,两种方法的阳性符合率为90.91%,阴性符合率为91.84%,总符合率为91.35%;413份临床猪血清样品中LI的阳性检出率为59.81%(247/413),表明LI在黑龙江、吉林等地区的猪群中普遍存在。本研究建立了检测LI抗体的间接ELISA方法,该方法特异性强、敏感性高、重复性与准确性均较好,为临床PI血清流行病学调查提供技术支持。

关键词：胞内劳森氏菌间接ELISA 抗体检测 LI0841重组蛋白

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Skin friction reduction characteristics of variable ovoid non-smooth surfaces

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2017年第1期18卷 59-66页

作者： Xiao-wen SONG peng-zhe LIN Rui LIU Pei ZHOU State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang University Hangzhou 310027 China

The use of bionic non-smooth surfaces is a popular approach for saving energy because of their drag reduction property. Conventional non-smooth structures include riblets and dimples. Inspired by sand dunes, a novel variable ovoid non-smooth structure is proposed in this study. The body of the variable ovoid dimple was designed based on three size parameters, the radius, semimajor, and depth, and a 3D model was created based on UG software. The constructed variable dimples were placed in a rectangular array on the bottom of a square tube model. Following ANSYS meshing, the grid model was imported into FLUENT, where the flow characteristics were calculated. Results of skin friction reduction were achieved and the effect of the design parameters on different variable ovoid dimples was obtained by orthogonal testing. Various aspects of the skin friction reduction mechanism were discussed including the distribution of velocity vectors, variation in boundary layer thickness, and pressure distribution.

关键词： Variable ovoid dimple Non-smooth surface Numerical simulation Skin friction reduction

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改进自适应LBG矢量量化算法在干涉高光谱图像压缩中的应用

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光谱学与光谱分析 2011年第4期31卷 1033-1037页

作者：温佳马彩文水鹏朗中国科学院西安光学精密机械研究所陕西西安710119 西安交通大学人工智能与机器人研究所陕西西安710049

在基于点到线模型扩展LBG(linde-buzo-gray algorithm)矢量量化算法的基础上,提出了一种更为高效的新型自适应LBG矢量量化算法,并给出了该算法在干涉高光谱图像无损压缩中的实际压缩方案。该算法在LBG算法码书中利用点到线的垂线关系基... 详细信息

在基于点到线模型扩展LBG(linde-buzo-gray algorithm)矢量量化算法的基础上,提出了一种更为高效的新型自适应LBG矢量量化算法,并给出了该算法在干涉高光谱图像无损压缩中的实际压缩方案。该算法在LBG算法码书中利用点到线的垂线关系基础上进行了改进,执行进一步的自适应化迭代进而获得了更小的残差。将自适应LBG矢量量化算法应用于干涉高光谱LASIS(large aperture static imaging spectrome-ter)图像数据无损压缩中,实验结果表明,该方法比原有的扩展LBG矢量量化算法在无损压缩性能上有显著的提高,并且在与LASIS图像双向预测结合后,点到线模型扩展LBG矢量量化算法的压缩比相对于传统LBG算法有所下降,而采用本文提出算法的压缩比则有明显的提高。

关键词：干涉高光谱 LASIS 图像编码 LBG矢量量化

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基于Yolov5的交通信号灯智能识别程序开发

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智能城市 2024年第3期10卷 18-21页

作者：郑国荣张尊栋赵文芊柏卓茁贾菲儿北方工业大学电气与控制工程学院北京100144

交通信号检测是智能汽车识别交通环境的一项重要辅助技术,现有的算法能够解决单一交叉口环境下的信号检测问题,但需要在十字路口的复杂交通环境中提高算法的精度和干扰可靠性。文章以one-stage目标检测算法Yolov5的应用为研究基础,实现... 详细信息

交通信号检测是智能汽车识别交通环境的一项重要辅助技术,现有的算法能够解决单一交叉口环境下的信号检测问题,但需要在十字路口的复杂交通环境中提高算法的精度和干扰可靠性。文章以one-stage目标检测算法Yolov5的应用为研究基础,实现多场景下的交通信号灯自动检测与识别,使用Labeling进行图片标注,通过镜像、裁剪、反转、等运行增强数据集,不断地调参实验与迭代模型训练,目标检测精度达到80%。

关键词：目标检测 Yolov5 Labeling图片标注模型训练

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Soil CO_2 Flux in Different Types of Forests Under a Subtropical Microclimatic Environment

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Pedosphere 2014年第2期24卷 243-250页

作者： YAN wen-De XU Wang-Ming CHEN Xiao-Yong TIAN Da-Lun peng Yuan-Ying ZHEN Wei ZHANG Cao XU Jie Forest Ecology Program College of Life Science and TechnologyCentral South University of Forestry and Technology National Engineering Laboratory for Applied Forest Ecological Technology in Southern China State Forestry Administration of China Division of Science College of Arts and SciencesGovernors State UniversityUniversity Park Natural Sciences Division College of DuPage

The flux of carbon dioxide （CO2） from soil surface presents an important component of carbon （C） cycle in terrestrial ecosystems and is controlled by a number of biotic and abiotic factors. In order to better understand characteristics of soil CO2 flux （FCO2） in subtropical forests, soil FCO2 rates were quantified in five adjacent forest types （camphor tree forest, Masson pine forest, mixed camphor tree and Masson pine forest, Chinese sweet gum forest, and slash pine forest） at the Tianjiling National Park in Changsha, Hunan Province, in subtropical China, from January to December 2010. The influences of soil temperature （Tsoil）, volumetric soil water content （0soiI）, soil pH, soil organic carbon （SOC） and soil C/nitrogen （N） ratio on soil FCO2 rates were also investigated. The annual mean soil FCO2 rate varied with the forest types. The soil FCO2 rate was the highest in the camphor tree forest （3.53 ± 0.51 μmol m-2 s-I）, followed by, in order, the mixed, Masson pine, Chinese sweet gum, and slash pine forests （1.53 ± 0.25 μmol m-2 sl）. Soil FCO2 rates from the five forest types followed a similar seasonal pattern with the maximum values occurring in summer （July and August） and the minimum values during winter （December and January）. Soil FCO2 rates were correlated to Tsoil and 0soil, but the relationships were only significant for Tsoil. No correlations were found between soil FCO2 rates and other selected soil properties, such as soil pH, SOC, and C/N ratio, in the examined forest types. Our results indicated that soil FCO2 rates were much higher in the evergreen broadleaved forest than coniferous forest under the same microclimatic environment in the study region.

关键词： broadleaved forest carbon coniferous forest soil temperature soil water content

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Value of urine IL-8, NGAL and KIM-1 for the early diagnosis of acute kidney injury in patients with ureteroscopic lithotripsy related urosepsis

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Chinese Journal of Traumatology 2022年第1期25卷 27-31页

作者： Dan Tan Liang Zhao Wei peng Fang-Hao Wu Guo-Bin Zhang Bo Yang wen-qian Huo Department of Urology The General Hospital of Chongqing Steel CompanyChongqing400081China

Purpose::To investigate the clinical value of urine interleukin-18(IL-8),neutrophil gelatinase-associated lipocalin(NGAL)and kidney injury molecule-1(KIM-1)for the early diagnosis of acute kidney injury(AKI)in patients with ureteroscopic lithotripsy(URL)related ***::A retrospective study was carried out in 157 patients with urosepsis after *** patients were divided into AKI group and non-AKI group according to the Kidigo guideline and urine IL-8,NGAL and KIM-1 levels were detected by enzyme-linked immunosorbent assay at 0,4,12,24 and 48 h after the *** operating characteristic curve(ROC)was used to evaluate the diagnostic value of these three biomarkers for postoperative ***::The level of urine IL-8,NGAL and KIM-1 in AKI group was significantly higher than that in non-AKI group at 4,12,24 and 48 h(p<0.01).The ROC analysis showed the combined detection of urine IL-8,NGAL and KIM-1 at 12 h had a larger area under curve(AUC)than a single marker(0.997,95%CI:0.991-0.998),and the sensitivity and specificity were 98.2%and 96.7%,*** correlation analysis showed that the levels of urine NGAL at 4,12,24 and 48 h in AKI patients were positively correlated with the levels of urine KIM-1 and IL-18(p<0.01).Conclusion::AKI could be quickly recognized by the elevated level of urine IL-8,NGAL and KIM-1 in patients with URL-related *** detection of the three urine biomarkers at 12 h after surgery had a better diagnostic performance,which may be an important reference for the early diagnosis of AKI.

关键词： Urosepsis Ureteroscopic lithotripsy Acute kidney injury Early diagnosis lnterleukin-18 Neutrophil gelatinase-associated lipocalin Kidney injury molecule-1

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Yield Evaluation of Twenty-Eight Alfalfa Cultivars in Hebei Province of China

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Journal of Integrative Agriculture 2014年第10期13卷 2260-2267页

作者： ZHANG Tie-jun KANG Jun-mei GUO wen-shan ZHAO Zhong-xiang XU Yu-peng YAN Xu-dong YANG Qing-chuan Institute of Animal Science Chinese Academy of Agricultural Sciences Cangzhou Technical College

Cultivar selection is important for alfalfa （Medicago sativa L.） hay production. From 2009 to 2012, a field study was conducted to evaluate the dry matter yield （DMY） of 28 cultivars in Cangzhou District of Hebei Province, China, and to determine the most suitable cultivars for this province and other zones with similar climate conditions. 28 alfalfa cultivars were sown in late March of 2009 and were harvested for hay four times in each subsequent year. The results showed that the climatic conditions resulted in significant differences in annual DMY among years, with the second year being the highest and the first year the lowest. The top five cultivars with the highest total DMY were L2750 （62.75 t ha^-1）, Hom （62.72 t ha^-1）, 86-266 （61.55 t ha^-1）, German （61.44 t ha^-1） and Zhongmu 1 （61.18 t ha^-1）, respectively. Across all four years, first harvest had the highest ratios to annual DMY except the cultivar of Rambler, while the fourth harvest had the lowest ratio. There were positive correlation relationships between DMY of each harvest and annual DMY, and the correlation coefficients were all significant in four years. And the path coefficients of first harvest were always the highest in four years. The qualities showed small variations among these cultivars and the cultivar L3750 presented the highest crude protein in both years. Crude protein had significant positive correlation with relative feed value （RFV） in both years while crude fiber had significant negative correlation with RFV and crude fiber.

关键词： alfalfa cultivars yield forage quality

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Serum levels of mi RNA in patients with hepatitis B virus-associated acute-on-chronic liver failure

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Hepatobiliary & Pancreatic Diseases International 2018年第2期17卷 126-132页

作者： Ya wen Shi-Fang peng Lei Fu Xiao-Yu Fu Da-Xian Wu Bing-Jie Liu De-Ming Tan Yi Ouyang Key Laboratory of Hunan Viral Hepatitis Department of Infectious Disease Xiangya Hospital Central South University

Background: Hepatitis B virus(HBV)-associated acute-on-chronic liver failure(HBV-ACLF) is a lifethreatening condition and its exact pathophysiology and progression remain unclear. The present study aimed to assess the role of serum mi RNAs in the evaluation of HBV-ACLF and to develop a model to predict the outcomes for ***: Serum was collected from 41 chronic hepatitis B and 55 HBV-ACLF patients in addition to30 chronic asymptomatic HBV carriers as controls. The mi RNAs expressions were measured by real-time quantitative PCR(q-PCR). Statistical analyses were conducted to assess the ability of differentially expressed mi RNAs and other prognostic factors in identifying ACLF prognosis and to develop a new predictive ***: Real-time q-PCR indicated that serum miR-146 a-5 p, mi R-122-3 p and mi R-328-3 p levels were significantly upregulated in ACLF patients compared to chronic hepatitis B and chronic asymptomatic HBV carriers patients. In addition, multivariate regression analyses indicated that Na+, INR, gastrointestinal bleeding and mi R-122-3 p are all independent factors that are reliable and sensitive to the prognosis of HBV-ACLF. Therefore, we developed a new model for the prediction of HBV-ACLF disease state: Y = 0.402 × Na+-1.72 × INR-4.963 × gastrointestinal bleeding(Yes = 0; No = 1)-0.278 ×(mi R-122-3 p) + 50.449. The predictive accuracy of the model was 95.3% and the area under the receiver operating characteristic curve(AUROC) was ***: Expression levels of these mi RNAs(miR-146 a-5 p, mi R-122-3 p and mi R-328-3 p) positively correlate with the severity of liver inflammation in patients with ACLF and may be useful to predict HBV-ACLF severity.

关键词： MicroRNA Hepatitis B virus Acute-on-chronic liver failure Prognosis

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