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A Confocal Endoscope for Cellular Imaging

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Engineering 2015年第3期1卷 351-360页

作者： Jiafu wang Min Yang Li Yang Yun Zhang Jing Yuan qian Liu Xiaohua Hou Ling fu Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology MoE Key Laboratory for Biomedical Photonics Department of Biomedical Engineering Huazhong University of Science and Technology Division of Gastroenterology Union Hospital Tongji Medical College Huazhong University of Science and Technology Mo E Key Laboratory for Biomedical Photonics Department of Biomedical Engineering Huazhong University of Science and Technology

Since its inception, endoscopy has aimed to establish an immediate diagnosis that is virtually consistent with a histologic diagnosis. In the past decade, confocal laser scanning microscopy has been brought into endoscopy, thus enabling in vivo microscopic tissue visualization with a magnification and resolution comparable to that obtained with the ex vivo microscopy of histological specimens. The major challenge in the development of instrumentation lies in the miniaturization of a fiber-optic probe for microscopic imaging with micron-scale resolution. Here, we present the design and construction of a confocal endoscope based on a fiber bundle with 1.4-μm lateral resolution and 8-frames per second(fps) imaging speed. The fiber-optic probe has a diameter of 2.6 mm that is compatible with the biopsy channel of a conventional endoscope. The prototype of a confocal endoscope has been used to observe epithelial cells of the gastrointestinal tracts of mice and will be further demonstrated in clinical trials. In addition, the confocal endoscope can be used for translational studies of epithelial function in order to monitor how molecules work and how cells interact in their natural environment.

关键词： cellular resolution confocal endoscopy optical biopsy

Emerging insights into heterotrimeric G protein signaling in plants

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Journal of Genetics and Genomics 2016年第8期43卷 495-502页

作者： Quan Xu Mingzhu Zhao Kun Wu Xiangdong fu qian Liu Rice Research Institute of Shenyang Agricultural University Key Laboratory of Northern Japonica Rice Genetics and BreedingMinistry of Education and Liaoning ProvinceKey Laboratory of Northeast Rice Biology and Genetics and BreedingMinistry of Agriculture The State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Heterotrimeric guanine nucleotide-binding protein （G protein） signaling is an evolutionarily conserved mechanism in diverse eukaryotic organisms. In plants, the repertoire of the heterotrimeric G protein complex, which is composed of the Ga, Gβ, and Gy subunits, is much simpler than that in metazoans, and the identity of typical G protein-coupled receptors （GPCRs） together with their ligands still remains unclear. Comparative phenotypic analysis in Arabidopsis and rice plants using gain- and loss-of-function mutants of G protein components revealed that heterotrimeric G protein signaling plays important roles in a wide variety of plant growth and developmental processes. Grain yield is a complex trait determined by quantitative trait loci （QTL） and is influenced by soil nitrogen availability and environmental changes. Recent studies have shown that the manipulation of two non-canonical Gy subunits, GS3 （GRAIN SIZE 3） and DEP1 （DENSE AND ERECT PANICLE 1）, represents new strategies to simultaneously increase grain yield and nitrogen use efficiency in rice. This review discusses the latest advances in our understanding of the heterotrimeric G protein signal transduction pathway and its application in improving yield and stress tolerance in crops.

关键词： G protein signalingNon-canonical G subunitsDEP1GS3Yield potential

Comparison of the toxicity of silver nanoparticles and silver ions on the growth of terrestrial plant model Arabidopsis thaliana

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Journal of Environmental Sciences 2013年第9期25卷 1947-1955页

作者： Haifeng qian Xiaofeng Peng Xiao Han Jie Ren Liwei Sun Zhengwei fu College of Biological and Environmental Engineering Zhejiang University of Technology

Silver nanoparticles （AgNPs） are one of the most widely used nanomaterials, but the mechanism of AgNP toxicity in terrestrial plants is still unclear. We compared the toxic effects of AgNPs and Ag＋ on Arabidopsis thaliana at the physiological, ultrastructural and molecular levels. AgNPs did not affect seed germination; however, they showed stronger inhibitory effect on root elongation than Ag＋ . The results of transmission electron microscopy and metal content analysis showed that AgNPs could be accumulated in leaves. These absorbed AgNPs disrupted the thylakoid membrane structure and decreased chlorophyll content, which can inhibit plant growth. By comparison, a small amount of Ag＋ was absorbed by seedlings, and it did not pronouncedly affect chloroplast structure and other metal ion absorption as AgNPs did. Compared with Ag＋ , AgNPs could alter the transcription of antioxidant and aquaporin genes, indicating that AgNPs changed the balance between the oxidant and antioxidant systems, and also affected the homeostasis of water and other small molecules within the plant body. All the data from physiological, ultrastructural and molecular levels suggest that AgNPs were more toxic than Ag＋ .

关键词： silver nanoparticles Ag+ Arabidopsis thaliana gene transcript antioxidant enzyme aquaporins

Isotope Effect in Collision Between Helium Atom and Hydrogen Molecule

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Chinese Physics Letters 1998年第1期15卷 19-20页

作者： YANG Xiang-dong ZHANG Ji-yan JlNG fu-qian Laboratory for Shock Wave and Detonation Physics Research Southwest Institute of Fluid PhysicsP.O.Box 523Chengdu 610003 Institute of Atomic and Molecular Science under High Temperature and High Pressure Sichuan Union UniversityChengdu 610065

Rotationally inelastic He-H_(2),D_(2),T_(2) collision was studied *** cross sections were calculated by using the close coupling method for relative kinetic energies of 0.01 to 0.54 ***-Oppenheimer approximation was employed to determine the potential surface of He-D_(2),He-T_(2),and He-H_(2).Careful comparison between the cross sections of the isotopically substituted systems was carried out,and the regular changes of the total cross sections of the isotopically substituted He-H_(2) colliding systems were obtained.

关键词： substituted collision cross

Separation of mandelic acid and its derivatives with new immobilized cellulose chiral stationary phase

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第7期14卷 615-620页

作者： Jie ZHOU qian LIU Guang-jun fu Zhen-zhong ZHANG Key Laboratory of State Ministry of Education for Pharmaceutical Technology School of Pharmacy Zhengzhou University

A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of mandelic acid and their derivatives 2-chloromandelic acid, 4-hydroxymandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid. The enantiomers were separated by a CHIRALPAKIC (250 mm×4.6 mm, 5 μm). Mandelic acid, 4-methoxymandelic acid, and 3,4,5-trismethoxymandelic acid were baseline resolved (resolution factor (RS )=2.21, RS =2.14, and RS =3.70, respectively). In contrast, the enantioselectivities between CHIRALPAKIC and 2-chloromandelic acid and 4-hydroxymandelic acid investigated were low. By comparing the chromatographs of mandelic acid enantiomers and mandelic acid spiked with (R)-mandelic acid, it was determined that the first effluent was (R)-mandelic acid.

关键词： CHIRALPAKIC Enantiomeric separation High-performance liquid chromatography (HPLC) Mandelic acid Derivatives

Malicious Code Detection Model Based on Behavior Association

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Tsinghua Science and Technology 2014年第5期19卷 508-515页

作者： Lansheng Han Mengxiao qian Xingbo Xu Cai fu Hamza Kwisaba Lab.of Information Security School of Computer ScienceHuazhong University of Science and Technology

Malicious applications can be introduced to attack users and services so as to gain financial rewards, individuals＇ sensitive information, company and government intellectual property, and to gain remote control of systems. However, traditional methods of malicious code detection, such as signature detection, behavior detection, virtual machine detection, and heuristic detection, have various weaknesses which make them unreliable. This paper presents the existing technologies of malicious code detection and a malicious code detection model is proposed based on behavior association. The behavior points of malicious code are first extracted through API monitoring technology and integrated into the behavior; then a relation between behaviors is established according to data dependence. Next, a behavior association model is built up and a discrimination method is put forth using pushdown automation. Finally, the exact malicious code is taken as a sample to carry out an experiment on the behavior＇s capture, association, and discrimination, thus proving that the theoretical model is viable.

关键词： malicious code behavior monitor behavior association pushdown automation

Epigenetic remodeling by DNA glycosylases during rice reproduction

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Molecular Plant 2021年第9期14卷 1433-1435页

作者： Mingzhuo Li Qirui Cui Xiang-qian Zhang Tzung-fu Hsieh Department of Plant and Microbial Biology North Carolina State UniversityRaleighNC 27695USA Plants for Human Health Institute North Carolina State UniversityNorth Carolina Research CampusKannapolisNC 28081USA College of Forestry and Landscape Architecture South China Agricultural University510642 GuangzhouChina

Cytosine methylation is a covale nt modificatio n of DNA that regulates important processes in eukaryotic genomes,including gene transcription,transposon silencing,and genomic imprinting(Law and Jacobsen,2010).DNA methylation patterns are faithfully duplicated upon cell division to ensure genome integrity and to maintain lineage-specific cell ***,DNA methylation also needs to be dynamically reprogramed or reconfigured during development to allow establishment of new cellular identity and transcriptional state,which plays a prominent role in animal development and reproduction,and is increasingly being appreciated for reproductive success in flowering plants(Walker et al.,2018;Ono and Kinoshita,2021).

关键词： al. remodeling maintain

Green Revolution DELLAs: From translational reinitiation to future sustainable agriculture

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Molecular Plant 2021年第4期14卷 547-549页

作者： qian Liu Kun Wu Nicholas P.Harberd Xiangdong fu State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Department of Plant Sciences University of OxfordSouth Parks RoadOxford 0X13RBUK College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

The Green Revolution(GR)of the 1960s saw spectacular increases in cereal crop yields,enabled by widespread adoption of high-yielding semi-dwarf varieties and increased fertilizer ***-dwarfism is caused by mutant wheat Reduced height-1(Rht-1)and rice semi-dwarf1(sd1)alleles,respectively,which confer increased harvest index(grain to straw ratio)and reduced yield loss from“lodging”(flattening of crops by wind or rain).However,despite their obvious global food security benefits,the underlying mechanisms via which GR dwarfing alleles actually work remained *** began to change with the molecular discovery of Rht1(Peng et al.,1999).

关键词： dwarf crops cereal

A Privacy-Preserving Distributed Subgradient Algorithm for the Economic Dispatch Problem in Smart Grid

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IEEE/CAA Journal of Automatica Sinica 2023年第7期10卷 1625-1627页

作者： qian Xu Chutian Yu Xiang Yuan Zao fu Hongzhe Liu State Grid Zhejiang Economic Research Institute Hangzhou 310008China Beijing Yinshan Technology CO. LTD.Beijing 100871China

Dear Editor,This letter aims to establish a privacy-preserving distributed optimization algorithm by combining the consensus iteration by subgradients, which not only enables the privacy preservation of optimization but also guarantees the optimality of solutions with some bias *** the setting of distributed optimization, a network of nodes, having their own objective functions depending on the global agents' state, would like to distributedly optimize the sum of all objective functions through the local agent-to-agent information change.

关键词： optimization iteration preserving