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A historical review of hybrid rice breeding

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Journal.of Integrative Plant Biology 2024年第3期66卷 532-545页

作者： Xiaoming Zheng Fei Wei Cheng Cheng qian qian State Key laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Yazhouwan National laboratory Sanya City 572024China

The development of germplasm resources and advances in breeding methods have led to steady increases in yield and quality of rice (oryza sativa l.). Three milestones in the recent history of rice breeding have contributed to these increases: dwarf rice breeding, hybrid rice breeding, and super rice breeding. on the 50th anniversary of the success of three-line hybrid rice,we highlight important scientific discoveries in rice breeding that were made by Chinese scientists and summarize the broader history of the field. We discuss the strategies that could be used in the future to optimize rice breeding further in the hope that China will continue to play a leading role in international rice breeding.

关键词： Chinese experience dwarf breeding hybrid rice breeding super rice breeding

Unveiling a half-century mystery of molecular bases for three-line hybrid rice breeding system

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Journal.of Integrative Plant Biology 2024年第1期66卷 3-6页

作者： Xiaoming Zheng qian qian State Key laboratory of Crop Gene Resources and Breeding Chinese Academy of Agricultural SciencesBeijing 100081China Yazhouwan National laboratory No.8 Huanjin RoadYazhou DistrictSanya 572024China

Rice(oryza sativa l.)isa staplecropforhalf theworld's Ipopulation and an important contributor to world food *** discovery and application of cytoplasmic male sterility(CMS)and fertility restoration(Rf)genetic materials have al.owed the production of three-line hybrid rice(Kim and Zhang,2018),successfully harnessing heterosis in *** major CMS and/or Rf systems are commonly used to produce three-line hybrid rice:Wild Abortive(CMS-WA),Boroll(CMS-BT),and Honglian(CMS-Hl).Among these,the CMS-WA system is the most widely employed(Sattari et al.,2007;Chen and liu,2014;Huang et al.,2014).Several Rf genes from different CMS systems have been cloned in rice,including Rf4 for CMS-WA(Tang et al.,2014),Rf1a and Rf1b for CMS-BT(Wang et al.,2006),Rf5,and Rf6 for CMS-Hl(Huet al.,2012;Huang et al.,2015),and Rf19for CMS-FA(Jiang et al.,2022).A molecular mechanism and evolutionary model for the CMS-WA gene WA352c have al.o been reported(l.o et al.,2013;Tang et al.,2017).

关键词： breeding fertility Wild

Existence and asymptotics of normalized solutions for the logarithmic Schrödinger system

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Science China Mathematics 2024年第9期67卷 2019-2048页

作者： qian Zhang Wenming Zou Department of Mathematical Sciences Tsinghua UniversityBeijing 100084China

This paper is concerned with the fol.owing logarithmic Schrodinger system:{-Δu_(1)+ω_(1)u_(1)=u_(1)u_(1)logu_(1)^(2)+2p/p+q|u_(2)|^(q)|u_(1)|^(p-2)u_(1),-Δu_(2)+ω_(2)u_(2)=u_(2)u_(2)log u_(2)^(2)+2q/p+q|u_(1)|^(p)|u_(2)|^(q-2)u_(2),∫_(Ω)|u_(i)|^(2)dx=ρ_(i),i=1,2,(u_(1),u_(2))∈H_(0)^(1)(Ω;R^(2)),where Ω=R^(N)or Ω■R^(N)(N≥3)is a bounded smooth domain,andω_(i)R,μ_(i),ρ_(i)>0 for i=1,***,p,q≥1,and 2≤p+q≤2^(*),where 2^(*):=2N/*** using a Gagliardo-Nirenberg inequality and a careful estimation of u log u^(2),firstly,we provide a unified proof of the existence of the normalized ground state solution for all 2≤p+q≤2^(*).Secondly,we consider the stability of normalized ground state ***,we analyze the behavior of solutions for the Sobolev-subcritical case and pass to the limit as the exponent p+q approaches 2^(*).Notably,the uncertainty of the sign of u log u^(2)in(0,+∞)is one of the difficulties of this paper,and al.o one of the motivations we are interested *** particular,we can establish the existence of positive normalized ground state solutions for the Brézis-Nirenberg type problem with logarithmic perturbations(i.e.,p+q=2^(*)).In addition,our study includes proving the existence of solutions to the logarithmic type Bréis-Nirenberg problem with and without the l^(2)-*** ∫_(Ω)|u_(i)|^(2)dx=ρ_(i)(i=1,2)by two different methods,*** results seem to be the first result of the normalized solution of the coupled nonlinear Schrodinger system with logarithmic perturbations.

关键词： logarithmic Schrodinger system Brézis-Nirenberg problem normalized solution existence and stability behavior of solutions

Toward a rapid and convenient nanoplastic quantification method in laboratory-scale study based on fl.orescence intensity

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Frontiers of Environmental Science & Engineering 2024年第5期18卷 117-126页

作者： Ruiqi Yan Sen lin qian Ding lei Zhang Xia Yu Wentao Zhao qian Sui State Environmental Protection Key laboratory of Environmental Risk Assessment and Control.on Chemical Process School of Resources and Environmental EngineeringEast China University of Science and TechnologyShanghai200237China State Key laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai200092China Shanghai Institute of Pollution Control and Ecological Security Shanghai200092China

The thorough investigation of nanoplastics(NPs)in aqueous environments requires efficient and expeditious quantitative analytical methods that are sensitive to environmentally relevant NP concentrations and convenient to *** analysis-based quantitative methods have been acknowledged as effective and rapid approaches for quantifying NP concentrations in laboratory-scale ***,we compared three commonly used optical response indicators,namely fl.orescence intensity(FI),ultraviolet absorbance,and turbidity,to assess their performance in quantifying ***,orthogonal experiments were conducted to evaluate the influence of various water quality parameters on the preferred indicator-based quantification *** results revealed that FI exhibits the highest correlation coefficient(>0.99)with NP ***,the limit of quantification(loQ)for various types of NPs is exceptionally low,ranging from 0.0089 to 0.0584 mg/l in ultrapure water,well below environmentally relevant *** variations in water quality parameters such as pH,salinity,suspended solids(SS),and humic acid,a robust relationship between detectable FI and NP concentration was ***,an increased matrix,especially SS in water samples,results in an enhanced loQ for ***,the quantitative method remains applicable in real water bodies,especially in drinking water,with NP loQ as low as 0.0157–0.0711 mg/*** exceeds the previously reported detectable concentration for 100 nm NPs at 40µg/ml using surface-enhanced Raman *** study confirms the potential.of FI as a reliable indicator for the rapid quantification of NPs in aqueous environments,offering substantial advantages in terms of both convenience and cost-effectiveness.

关键词： Nanoplastic quantification Fl.orescence intensity Drinking water limit of quantification(loQ) Matrix effects

MIXED DISCoNTINUoUS GAlERKIN METHoD FoR QUASI-NEWToNIAN SToKES FloWS

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Journal.of Computational Mathematics 2024年第3期42卷 885-910页

作者： Yanxia qian Fei Wang Wenjing Yan School.of Mathematics and Statistics Xi'an Jiaotong UniversityXi'an 710049China School.of Mathematics and Statistics&State Key laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong UniversityXi'an 710049China

In this paper,we introduce and analyze an augmented mixed discontinuous Galerkin(MDG)method for a class of quasi-Newtonian Stokes *** the mixed formulation,the unknowns are strain rate,stress and velocity,which are approximated by a discontinuous piecewise polynomial triplet ■for k≥***,the discontinuous piecewise polynomial function spaces for the field of strain rate and the stress field are designed to be *** addition,the pressure is easily recovered through simple *** the benefit of the analysis,we enrich the MDG scheme with the constitutive equation relating the stress and the strain rate,so that the well-posedness of the augmented formulation is obtained by a nonlinear functional *** k≥0,we get the optimal convergence order for the stress in broken ■(div)-norm and velocity in l^(2)-***,the error estimates of the strain rate and the stress in-norm,and the pressure in l^(2)-norm are optimal under certain ***,several numerical examples are given to show the performance of the augmented MDG method and verify the theoretical *** evidence is provided to show that the orders of convergence are sharp.

关键词： Quasi-Newtonian fows Mixed discontinuous Galerkin method Symmetric strain rate Symmetric stress optimal convergence orders

lÉVY AREA ANAlYSIS AND PARAMETER ESTIMATIoN FoR FoU PRoCESSES VIA NoN-GEoMETRIC RoUGH PATH THEoRY

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Acta Mathematica Scientia 2024年第5期44卷 1609-1638页

作者： Zhongmin qian Xingcheng XU Mathematical Institute University of OxfordOxfordOX26GGUK Shanghai Artificial Intelligence laboratory Shanghai200232China School.of Mathematical Sciences Peking UniversityBeijing100871China

This paper addresses the estimation problem of an unknown drift parameter matrix for a fractional.ornstein-Uhlenbeck process in a multi-dimensional *** tackle this problem,we propose a novel approach based on rough path theory that al.ows us to construct pathwise rough path estimators from both continuous and discrete observations of a single *** approach is particularly suitable for high-frequency *** formulate the parameter estimators,we introduce a theory of pathwise Itôintegrals with respect to fractional Brownian *** establishing the regularity of fractional.ornstein-Uhlenbeck processes and analyzing the long-term behavior of the associated lévy area processes,we demonstrate that our estimators are strongly consistent and pathwise *** findings offer a new perspective on estimating the drift parameter matrix for fractional.ornstein-Uhlenbeck processes in multi-dimensional settings,and may have practical implications for fields including finance,economics,and engineering.

关键词： Itôintegration lévy area non-geometric rough path foU processes pathwise stability long time asymptotic high-frequency data

Brain circuits for retching-like behavior

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National Science Review 2024年第1期11卷 145-160页

作者： lifang Huo Zhimin Ye Meiling liu Ziqing He Meizhu Huang Dapeng li qian Wu qian Wang Xiaoqun Wang Peng Cao Ji Dong Congping Shang School.of Basic Medical Sciences Guangzhou National Laboratory The Fifth Afflliated Hospital Guangzhou Medical University Bioland laboratory (Guangzhou Regenerative Medicine and Health Guangdong laboratory) Department of Neurobiology School of Basic Medical Sciences Beijing Key Laboratory of Neural Regeneration and Repair Advanced Innovation Center for Human Brain Protection Capital Medical University State Key laboratory of Cognitive Neuroscience and learning IDG/McGovern Institute for Brain Research Beijing Normal University Changping life Science laboratory National Institute of Biological Sciences

Nausea and vomiting are important defensive responses to cope with pathogens and toxins that invade the body. The nucleus of the solitary tract(NTS) is important for initiating these responses. However, the molecular heterogeneities and cellular diversities of the NTS occlude a better understanding of these defensive responses. Here, we constructed the single-nucleus transcriptomic atlas of NTS cells and found multiple populations of NTS neurons that may be involved in these defensive responses. Among these, we identified Calbindin1-positive(Calb1+) NTS neurons that are molecularly distinct from Tac1+*** Calb1+neurons are critical for nausea and retching induced by cereulide; an emetic toxin secreted by Bacillus Cereus. Strikingly, we found that cereulide can directly modulate vagal sensory neurons that innervate Calb1+NTS neurons, a novel mechanism distinct from that for nausea and retching induced by Staphylococcal enterotoxin A. Together, our transcriptomic atlas of NTS neurons and the functional analyses revealed the neural mechanism for cereulide-induced retching-like behavior. These results demonstrate the molecular and cellular complexities in the brain that underlie defensive responses to the diversities of pathogens and toxins.

关键词： vomiting nausea nucleus of the solitary tract Calbindin1 neural circuits

Development of RPA-Cas12a-fl.orescence assay for rapid and reliable detection of human bocavirus 1

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Animal Models and Experimental Medicine 2024年第2期7卷 179-188页

作者： Weidong qian Xuefei Wang Ting Wang Jie Huang qian Zhang Yongdong li Si Chen School.of Food and Biological Engineering Shaanxi University of Science and TechnologyXi'anP.R.China Department of Dermatology Huazhong University of Science and Technology Union Shenzhen HospitalShenzhenP.R.China Ningbo Municipal Center for Disease Control and Prevention NingboP.R.China Medical College of Shenzhen University ShenzhenP.R.China

Human bocavirus(HBoV)1 is considered an important pathogen that mainly affects infants aged 6–24 months,but preventing viral transmission in resource-limited regions through rapid and affordable on-site diagnosis of individuals with early infection of HBoV1 remains somewhat ***,we present a novel faster,lower cost,reliable method for the detection of HBoV1,which integrates a recombinase polymerase amplification(RPA)assay with the CRISPR/Cas12a system,designated the RPA-Cas12a-fl.orescence *** RPA-Cas12a-fl.orescence system can specifically detect target gene levels as low as 0.5 copies of HBoV1 plasmid DNA per microliter within 40 min at 37℃without the need for sophisticated *** method al.o demonstrates excellent specificity without cross-reactivity to non-target ***,the method was appraised using 28 clinical samples,and displayed high accuracy with positive and negative predictive agreement of 90.9%and 100%,***,our proposed rapid and sensitive HBoV1 detection method,the RPA-Cas12a-fl.orescence assay,shows promising potential for early on-site diagnosis of HBoV1 infection in the fields of public health and health *** established RPA-Cas12a-fl.orescence assay is rapid and reliable method for human bocavirus 1 *** RPA-Cas12a-fl.orescence assay can be completed within 40 min with robust specificity and sensitivity of 0.5 copies/μl.

关键词： CRISPR-Cas12a detection human bocavirus 1 on-site diagnosis recombinase polymerase amplification

Porous TiFe_(2) intermetallic compound fabricated via elemental powder reactive synthesis

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International Journal.of Minerals,Metallurgy and Materials 2024年第4期31卷 764-772页

作者： qian Zhao Zhenli He Yuehui He Yue Qiu Zhonghe Wang Yao Jiang State Key laboratory of Powder Metallurgy Powder Metallurgy Research InstituteCentral South UniversityChangsha 410083China

Porous intermetallics show potential in the field of filtration and separation as well as in the field of ***,porous Ti Fe2intermetallics were fabricated by the reactive synthesis of elemental *** phase transformation and pore formation of porous TiFe2intermetallics were investigated,and its corrosion behavior and hydrogen evolution reaction(HER)performance in alkali solution were *** TiFe2intermetallics with porosity in the range of 34.4%-56.4%were synthesized by the diffusion reaction of Ti and Fe elements,and the pore formation of porous TiFe2intermetallic compound is the result of a combination of the bridging effect and the Kirkendall *** porous TiFe2samples exhibit better corrosion resistance compared with porous 316l stainless steel,which is related to the formation of uniform nanosheets on the surface that hinder further corrosion,and porous TiFe2electrode shows the overpotential.of 220.6 and 295.6 mV at 10 and 100 mA·cm-2,suggesting a good catalytic *** synthesized porous Fe-based intermetallic has a controllable pore structure as well as excellent corrosion resistance,showing its potential in the field of filtration and separation.

关键词： TiFe2 intermetallic compound porous materials reactive synthesis corrosion behavior hydrogen evolution reaction