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A rice geranylgeranyl reductase is essential for chloroplast development

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Journal of Integrative Agriculture 2021年第10期20卷 2592-2600页

作者： LIU Xi YI Xin YANG Yan-rong huang qian-qian Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake Huaiyin Normal UniversityHuai’an 223300P.R.China

Chloroplasts are essential for plant photosynthesis and *** genes have been identified that regulate plant chloroplast ***,it is not known at a molecular level how these genes regulate chloroplast *** this study,we isolated a mutant ygl2(yellow-green leaf2)that exhibited a pigment-defective ***2 encodes a geranylgeranyl reductase,and in mutant ygl2,there was a single base change(T1361G)located in the third exon of YGL2 that resulted in a missense mutation(L454R)in the encoded *** electron microscopy revealed that chloroplast development was impaired in the ygl2 *** expression levels of plastid-encoded genes were significantly altered in the ygl2 ***,in a yeast two-hybrid assay,we found that YGL2 interacted with the RNA editing factor MORF8.

关键词： rice(Oryza sativa L.) chloroplast YGL2 geranylgeranyl reductase

Brown and beige fat： the metabolic function, induction, and therapeutic potential

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Frontiers of Medicine 2015年第2期9卷 162-172页

作者： Shuwen qian Haiyan huang Qiqun Tang Key Laboratory of Metabolism and Molecular Medicine the Ministry of Education Department of Biochemistry and Molecular Biology Fudan University Shanghai Medical College Shanghai 200032 China

Adipose tissue is an important organ for energy homeostasis. White adipose tissue stores energy in the form of triglycerides, whereas brown adipocytes and recently identified beige adipocytes are specialized in dissipating energy by thermogenesis or contribution to dispose glucose and clear triglycerides in blood. The inverse correlation between the brown adipose tissue activity and body mass suggests its protective role against body fat accumulation. Thus, recruitment and activation of brown or beige adipose tissue become particularly appealing targets for increasing energy expenditure. Angiogenesis and sympathetic nerve signals are the fundamental determinants for brown and beige adipose tissue development, as well as for their metabolic functions. Secretary factors including BMPs can induce the development, the activation of brown or beige adipose tissue, which seem to be promising for therapeutic development.

关键词： brown adipocyte beige adipocyte metabolism obesity

Experimental Study on Flow Boiling of Deionized Water in a Horizontal Long Small Channel

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Journal of Thermal Science 2018年第2期27卷 157-166页

作者： huang qian JIA Li DANG Chao YANG Lixin Institute of Thermal Engineering School of Mechanical Electronic and Control Engineering Beijing Jiaotong University Beijing 100044 China Beijing Key Laboratory of Flow and Heat Transfer of Phase Changing in Micro and Small Scale Beijing 100044 China

In this paper,an experimental investigation on the flow boiling heat transfer in a horizontal long mini-channel was carried out. The mini-channel was with 2 mm wide and 1 mm deep and 900 mm long. The material of the mini-channel was stainless. The working fluid was deionized water. The experiments were conducted with the conditions of inlet pressure in the range of 0.2~0.5 MPa, mass flux in the range of 196.57-548.96 kg/m2 s, and the outlet vapor quality in the range of 0.2 to 1. The heat flux was in the range of 292.86 kW/m2 to 788.48 kW/m2,respectively. The influences of mass flux and heat flux were studied. At a certain mass flow rate, the local heat transfer coefficient increased with the increase of the heat flux. If dry-out occurred in the mini-channel, the heat transfer coefficient decreased. At the same heat flux, the local heat transfer coefficient would depend on the mass flux. It would increase with the mass flux in a certain range,and then decrease if the mass flux was beyond this range. Experimental data were compared with the results of previous studies. Flow visualization and measurements were conducted to identify flow regime transitions. Results showed that there were eight different kinds of flow patterns occurring during the flow boiling. It was found that flow pattern had a significant effect on heat transfer.

关键词： Flow boiling Long mini-channel Visualization Evaporator Heat transfer coefficient

Thermal residual stress analysis of coated diamond grits

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International Journal of Minerals,Metallurgy and Materials 2009年第2期16卷 215-219页

作者： Zi-qian huang Bo Xian Yue-hui He Bai-yun huang State Key Laboratory of Powder Metallurgy Central South University Changsha 410083 China Zhejiang Huayou Cobalt Co. Ltd. Tongxiang 314500 China

Residual stresses of coated diamond grits were analyzed by a finite element unit cell model. Diamond grits coated with four types of metals, W, Mo, Ti, and Cr, were considered. The numerical results show that compressive stress occurs in the diamond particles and tensile stress occurs in the metal matrix; compressive stress is concentrated in the diamond sharp comer; interface stresses decrease by more than 1000 MPa with a metal interlayer; plastic deformation of the matrix begins near the sharp corner of diamond grits and extends toward the peripheral zone. Stress concentration dramatically decreases due to plastic deformation of the matrix. The deposition of transition metals on a diamond surface can dramatically promote the adhesion between diamond grits and the metal bond.

关键词： diamond grit residual stress interlayer finite element method

The parasitic dinoflagellate Hematodinium infects marine crustaceans

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Marine Life Science & Technology 2021年第3期3卷 313-325页

作者： Caiwen Li Meng Li qian huang CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao266071China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266237China Centre for Ocean Mega-Science Chinese Academy of SciencesQingdao266071China University of Chinese Academy of Sciences Beijing100049China

Hematodinium is a type of parasitic dinoflagellate that infects marine crustaceans *** parasite lives mainly in the hemolymph or hemocoels of affected hosts,and results in mortalities due to malfunction or loss of functions of major *** recent years,the parasite had developed into an emerging epidemic pathogen not only affecting wild populations of economically valuable marine crustaceans in western countries but also the sustainable yield of aquaculture of major crabs in *** epidemics of the parasitic diseases expanded recently in the coastal waters of China,and caused frequent outbreaks in aquaculture of major crab species,especially Portunus trituberculatus and Scylla *** addition,the pathogen infected two species of co-cultured shrimps and multiple cohabitating wild crabs,implying it is a significant threat to the sustainable culture of commercially valuable marine *** particular,the polyculture system that is widely used along the coast of China may facilitate the spread and transmission of the ***,to provide a better understanding of the biological and ecological characteristics of the parasitic dinoflagellate and highlight important directions for future research,we have reviewed the current knowledge on the taxonomy,life cycle,pathogenesis,transmission and epidemiology of Hematodinium ***,ecological countermeasures have been proposed for the prevention and control of the emerging infectious disease.

关键词： Parasitic dinoflagellate Diversity Life cycle Pathogenesis Outbreak mechanism Ecological control

The Chang’E-1 orbiter plays a distinctive role in China’s first successful selenodetic lunar mission

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Science China(Physics,Mechanics & Astronomy) 2011年第12期54卷 2130-2144页

作者： PING JinSong SU XiaoLi huang qian YAN JianGuo National Astronomical Observatory Chinese Academy of Sciences Beijing 100012 China Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China State Key Laboratory of lnformation Engineering in Surveying Mapping and Remote Sensing Wuhan University Wuhan 430070 China

The first Chinese lunar orbiter Chang'E-1 is a successful mission with many fruitful results obtained in various disciplines. The scientific data acquired by the Chang'E-1 payloads can benefit studies of the lunar origin and evolution, as well as other relevant research areas, after careful validation of the data. Among the new results, the Chang'E-1 selenodetic products are continually uncovering characteristics of the lunar surface, undersurface and inner structure. Successful lunar orbiters such as the Clementine, Lunar Prospector, KAGUYA/SELENE, Chang'E-1, Lunar Reconnaissance Orbiter and GRAIL have been revealing, with increasing clarity, global selenodetic characteristics with state-of-the-art fine resolution and high precision. In particular, the Chang'E-1 plays an important distinctive role in selenodetic exploration through enhancing lunar topography and gravity models. The gravity model has been successfully improved with a factor of two after applying the Chang'E-1 long-wavelength tracking data. Using the new models, some medium-scale lunar surface characteristics such as basins and volcanoes have been identified. Furthermore, the old mascon basins of Bouguer, gravity anomaly and craters have been discovered with the Chang'E-1 selenodetic data.

关键词： Chang’E-1 lunar orbiter lunar science selenodesy topography gravity

Identification and geochemical significance of unusual C24 tetracyclic terpanes in Shahejie Formation source rocks in the Bozhong subbasin,Bohai Bay Basin

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Petroleum Science 2022年第5期19卷 1993-2003页

作者： Ning Wang Yao-Hui Xu Fei-Long Wang Yan Liu qian huang Xing huang Key Laboratory of Exploration Technologies for Oil and Gas Resources Ministry of EducationYangtze UniversityWuhan430100China Hubei Key Laboratory of Petroleum Geochemistry and Environment(Yangtze University) Wuhan430100China Bohai Petroleum Research Institute Tianjin BranchCNOOC(China)Ltd.Tianjin300452China

C_(24)tetracyclic terpanes are common compounds in source rocks and crude oils,and C_(24)17,21-secohopane is the most common and widely used source-related *** this study,three unusual C_(24)tetracyclic terpanes were detected on the m/z 191 chromatogram of saturated hydrocarbons in the Shahejie Formation source rocks in the Bozhong *** on the mass spectra characteristics,diagnostic ion fragments,retention time and comparisons with published literature,three unusual C_(24)tetracyclic terpanes were identified as 10β(H)-des-A-oleanane,10β(H)-des-A-lupane and C_(24)*** the best of our knowledge,this is the first study to detect and publicly report these three compounds in source rock samples from the Shahejie Formation of the Bozhong subbasin,Bohai Bay *** results indicated that 10β(H)-des-A-oleanane and 10β(H)-des-A-lupane likely originated from terre strial angiosperms,while C_(24)des-A-hopane likely originated fro m prokaryotic o *** rrestrial angiosperms provide the material basis for the generation of compounds A and B,and the distribution and concentration of these two compounds are affected by thermal *** the low maturity stage(0.5%

关键词： Unusual tetracyclic terpanes 10β(H)-des-A-oleanane 10β(H)-des-A-lupane Geochemical significance Shahejie Formation Bozhong subbasin

Ammonia volatilization from a Chinese cabbage field under different nitrogen treatments in the Taihu Lake Basin, China

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Journal of Environmental Sciences 2015年第12期27卷 14-23页

作者： Linan Shan Yunfeng He Jie Chen qian huang Hongcai Wang College of Environmental and Resource Science Zhejiang University Ministry of Agriculture Key Laboratory of Non-point Source Pollution Control

Ammonia（NH3） volatilization is a major pathway of nitrogen（N） loss from soil-crop *** vegetable cultivation is one of the most important agricultural land uses worldwide,a deeper understanding of NH3 volatilization is necessary in vegetable production *** therefore conducted a 3-year（2010-2012） field experiment to characterize NH3 volatilization and evaluate the effect of different N fertilizer treatments on this process during the growth period of Chinese *** volatilization rate,rainfall,soil water content,p H,and soil NH4~＋were measured during the growth *** results showed that NH3 volatilization was significantly and positively correlated to topsoil p H and NH4＋*** factors and fertilization method also significantly affected NH3 ***,organic fertilizer（OF） increased NH3 volatilization by 11.77%-18.46%,compared to conventional fertilizer（CF,urea）,while organic-inorganic compound fertilizer（OIF） reduced NH3 volatilization by 8.82%-12.67% compared to ***,slow-release fertilizers had significantly positive effects on controlling NH3 volatilization,with a 60.73%-68.80% reduction for sulfur-coated urea（SCU）,a 71.85%-78.97% reduction for biological Carbon Power~？ urea（BCU）,and a 77.66%-83.12% reduction for bulk-blend controlled-release fertilizer（BBCRF）relative to *** study provides much needed baseline information,which will help in fertilizer choice and management practices to reduce NH3 volatilization and encourage the development of new strategies for vegetable planting.

关键词： Ammonia volatilization Nitrogen fertilizer Slow-release fertilizer Vegetable soil

Neuropsychiatric Symptoms,Parenting Stress and Social Support in Chinese Mothers of Children with Autism Spectrum Disorder

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Current Medical Science 2019年第2期39卷 291-297页

作者： Xiao-qian huang Hong ZHANG Shan CHEN Department of Neurology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Teaching and Research Section of Neuropsychiatry Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Pediatrics Union Hospital of Fujian Medical UniversityFuzhou 350001China

Although little is known about the current situation regarding autism spectrum disorder(ASD)in main land China,psychiatric disorders are common among Chinese mothers of preschool children with *** studies showed ASD child's behavioral symptoms,maternal anxiety,and maternal depressive symptoms were associated with overall parenting stress in northern *** the present study,we retrospectively analyzed medical records at the hospital related to neuropsychiatric symptoms,parenting stress and social support in mothers of children with ASD from southern China.A total of 80 mothers of children with ASD were *** them,34 mothers were in low-functioning ASD group(L-ASD group)and 46 mothers were in highfunctioning ASD group(H-ASD group).Identification of the ASD cases was confirmed with a Revised Autism Diagnostic *** symptoms,parenting stress and social support were measured by neuropsychiatric inventory(NPI),parenting stress index short form(PSISF),and multi-dimensional scale of perceived social support(MSPSS).Total mean score of the NPI in the L-ASD group was significantly higher than that in the H-ASD group(P<0.01).The subscale scores of NPI,including depression,anxiety,apathy,irritability,agitation,night time behavior disturbances and change in appetite were significantly higher in the L-ASD group than those in the H-ASD group(P<0.01 or P<0.05).Meanwhile,the total PSI-SF scores and the scores of parental distress(PD),parental-child dysfunctional interaction(PCDI)and difficult child(DC)in the L-ASD group were significantly higher than those in the H-ASD group(P<0.01 or P<0.05).The total score of MSPSS was also higher in the L-ASD group than in the H-ASD group(P<0.01).This study goes further to show the neuropsychiatric symptoms and parenting stress are significantly higher in mothers of children with ASD,and more social supports are needed for mothers of children with ASD from southern China,especially for mothers of children

关键词： autism spectrum disorder neuropsychiatric symptoms parenting stress social support mothers