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The use of machine learning to predict the effects of cryoprotective agents on the GelMA-based bioinks used in extrusion cryobioprinting

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Bio-Design and Manufacturing 2023年第4期6卷 464-477页

作者： qian qiao Xiang Zhang Zhenhao Yan Chuanyu Hou Juanli Zhang Yong He Na Zhao Shujie Yan Youping Gong qian Li School of Mechanics and Safety Engineering Zhengzhou UniversityZhengzhou 450001China State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou 310027China NMPA Key Laboratory for Quality Control of In Vitro Diagnostics Henan Institute of Medical Device InspectionsZhengzhou 450003China School of Mechanical Engineering Hangzhou Dianzi UniversityHangzhou 310018China National Center for International Joint Research of Micro-Nano Molding Technology Zhengzhou UniversityZhengzhou 450001China Key Laboratory for Micro Molding Technology of Henan Province Zhengzhou UniversityZhengzhou 450001China

Cryobioprinting has tremendous potential to solve problems to do with lack of shelf availability in traditional bioprinting by combining extrusion bioprinting and cryopreservation.In order to ensure the viability of cells in the frozen state and avoid the possible toxicity of dimethyl sulfoxide(DMSO),DMSO-free bioink design is critical for achieving successful cryobioprinting.A nontoxic gelatin methacryloyl-based bioink used in cryobioprinting is composed of cryoprotective agents(CPAs)and a buffer solution.The selection and ratio of CPAs in the bioink directly affect the survival of cells in the frozen state.However,the development of universal and efficient cryoprotective bioinks requires extensive experimentation.We first compared two commonly used CPA formulations via experiments in this study.Results show that the effect of using ethylene glycol as the permeable CPA was 6.07%better than that of glycerol.Two datasets were obtained and four machinelearning models were established to predict experimental outcomes.The predictive powers of multiple linear regression(MLR),decision tree(DT),random forest(RF),and artificial neural network(ANN)approaches were compared,suggesting an order of ANN>RF>DT>MLR.The final selected ANN model was then applied to another dataset.Results reveal that this machine-learning method can accurately predict the effects of cryoprotective bioinks composed of different CPAs.Outcomes also suggest that the formulations presented here have universality.Our findings are likely to greatly accelerate research and development on the use of bioinks for cryobioprinting.

关键词： Cryobioprinting Cryoprotective bioink 3D bioprinting Machine learning Artificial intelligence Prediction model

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走和平发展道路的现代化:理论渊源、价值向度与世界意义

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云南大学学报（社会科学版） 2024年第2期23卷 87-96页

作者：乔倩中国石油大学北京102249

走和平发展道路作为中国式现代化的五大特征之一,是中国共产党带领中国人民站在全球高度对中国式现代化宏伟蓝图做出的擘画,是以人为本的理念在世界范围内的丰富和拓展。首先,走和平发展道路的现代化是走和平、发展、合作、共赢的现代... 详细信息

走和平发展道路作为中国式现代化的五大特征之一,是中国共产党带领中国人民站在全球高度对中国式现代化宏伟蓝图做出的擘画,是以人为本的理念在世界范围内的丰富和拓展。首先,走和平发展道路的现代化是走和平、发展、合作、共赢的现代化之路,与人类命运共同体中所蕴含的“和平、发展、公平、正义、民主、自由”全人类共同价值高度契合。其次,人类命运共同体理念为和平发展道路的现代化提供全球层面发展观、安全观、生态观和文明观的价值引领,和平发展道路的现代化为构建人类命运共同体提供行之有效的实践路径,二者共同为化解世界发展难题、全球经济危机、生态环境危机等提供世界观和方法论的指导,也为人类社会进步、人类文明新形态的发展提供可资参考的道路模式。再次,走和平发展道路的现代化是对“中国威胁论”等谎言谬论的回击,深刻展现了大国责任和大国担当,对凝聚共促发展国际共识、构建新型全球发展伙伴关系等方面具有重大世界意义。

关键词：走和平发展道路的现代化中国式现代化人类命运共同体全人类共同价值

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全固态风冷式激光器散热设计及优化

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激光技术 2024年第2期48卷 235-239页

作者：乔迁赵虎谢兰强北京东方锐镭科技有限公司北京100102

为了解决输出能量不小于220 mJ、重复频率40 Hz风冷激光器连续工作的散热问题,采用强迫空冷的散热方式,通过热电制冷器对巴条进行温度控制。设计了散热方案,构建了散热器的3维模型,利用热仿真软件对激光器散热情况进行热仿真,分析其优... 详细信息

为了解决输出能量不小于220 mJ、重复频率40 Hz风冷激光器连续工作的散热问题,采用强迫空冷的散热方式,通过热电制冷器对巴条进行温度控制。设计了散热方案,构建了散热器的3维模型,利用热仿真软件对激光器散热情况进行热仿真,分析其优化结果并进行了实验验证。结果表明,在环境温度为55℃、重复频率为40 Hz、激光器输出能量大于220 mJ的情况下,设计制作的紫铜翅片散热器可满足激光器连续工作的散热要求,且有15%左右的设计余量;此热仿真方法与实际情况有很好的匹配效果,可用于后续的散热器设计指导。该研究为激光器更深层次的热设计提供了有效参考。

关键词：激光器散热器热设计仿真热电制冷器

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The WRKY transcription factor PlWRKY65 enhances the resistance of Paeonia lactiflora(herbaceous peony)to Alternaria tenuissima

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Horticulture Research 2020年第1期7卷 2092-2103页

作者： Xue Wang Junjie Li Jing Guo qian qiao Xianfeng Guo Yan Ma College of Forestry Shandong Agricultural UniversityNo.61Daizong Road271018 Tai’anShandongChina Shandong Provincial Research Center of Demonstration Engineering Technology for Urban and Rural Landscapes 271018 Tai’anShandongChina Shandong Institute of Pomology 271000 Tai’anShandongChina

In this study,the disease resistance gene PlWRKY65 was isolated from the leaves of Paeonia lactiflora and analyzed by bioinformatics methods,and the localization of the encoded protein was explored.Quantitative real-time PCR(qRTPCR)was also used to explore the response of this gene to Alternaria tenuissima.The results showed that the gene sequence contained multiple cis-acting elements involved in the response to hormone signaling molecules belonging to the IIe subgroup of the WRKY family,and the encoded proteins were located in the nucleus.The PlWRKY65 gene has a positive regulatory effect on A.tenuissima infection.After silencing the PlWRKY65 gene via virus-induced gene silencing(VIGS),it was found that the gene-silenced plants were more sensitive to A.tenuissima infection than the wild plants,exhibiting more severe infection symptoms and different degrees of changes in the expression of the pathogenesis-related(PR)genes.In addition,we showed that the endogenous jasmonic acid(JA)content of P.lactiflora was increased in response to A.tenuissima infection,whereas the salicylic acid(SA)content decreased.After PlWRKY65 gene silencing,the levels of the two hormones changed accordingly,indicating that PlWRKY65,acting as a disease resistance-related transcriptional activator,exerts a regulatory effect on JA and SA signals.This study lays the foundation for functional research on WRKY genes in P.lactiflora and for the discovery of candidate disease resistance genes.

关键词： genes resistance VIGS

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Erbium-ytterbium co-doped lithium niobate single-mode microdisk laser with an ultralow threshold of 1μW

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Chinese Optics Letters 2024年第4期22卷 48-52页

作者：李明慧高仁宏黎春桃管江林张海粟林锦添赵光辉乔迁汪旻乔玲玲邓莉程亚 Chinese Academy of Sciences(CAS)Shanghai 201800China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China XXL—The Extreme Optoelectromechanics Laboratory School of Physics and Electronic ScienceEast China Normal UniversityShanghai 200241China State Key Laboratory of Precision Spectroscopy East China Normal UniversityShanghai 200062China School of Physical Science and Technology Shanghai Tech UniversityShanghai 200031China Shanghai Research Center for Quantum Sciences Shanghai 201315China Hefei National Laboratory Hefei 230088China

We demonstrate single-mode microdisk lasers in the telecom band with ultralow thresholds on erbium-ytterbium co-doped thin-film lithium niobate(TFLN).The active microdisk was fabricated with high-Q factors by photolithography-assisted chemomechanical etching.Thanks to the erbium-ytterbium co-doping providing high optical gain,the ultralow loss nanostructuring,and the excitation of high-Q coherent polygon modes,which suppresses multimode lasing and allows high spatial mode overlap between pump and lasing modes,single-mode laser emission operating at 1530 nm wavelength was observed with an ultralow threshold,under a 980-nm-band optical pump.The threshold was measured as low as 1μW,which is one order of magnitude smaller than the best results previously reported in single-mode active TFLN microlasers.The conversion efficiency reaches 4.06×10^(-3),which is also the highest value reported in single-mode active TFLN microlasers.

关键词： lithium niobate microcavities microdisk lasers

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城市综合开发3.0模式下的一级项目管理问题及研究——以临平国际未来产居生态城综合开发项目为例

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价值工程 2024年第3期43卷 12-15页

作者：钱乔峰广州中医药大学第一附属医院广州510000

“十四五”期间,我国新型城镇化持续推进,城市综合开发3.0越来越成为城市发展模式的主导方向。城市综合开发的一级业务是基础,在众多部门线条中项目管理又是核心,项目管理是否有成效对项目成败起到至关重要的作用。面对实际推行中出现... 详细信息

“十四五”期间,我国新型城镇化持续推进,城市综合开发3.0越来越成为城市发展模式的主导方向。城市综合开发的一级业务是基础,在众多部门线条中项目管理又是核心,项目管理是否有成效对项目成败起到至关重要的作用。面对实际推行中出现的问题分析,结合临平国际未来产居生态城综合开发项目实践,本文探索城市综合开发一级项目管理的要点,试图以此提升项目管理的质量与水平,实现项目的成功实施和高质量交付,促进城市综合开发模式的持续完善。

关键词：城市综合开发项目管理问题要点

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Signaling network controlling ROP-mediated tip growth in Arabidopsis and beyond

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Plant Communications 2023年第1期4卷 198-208页

作者： En Li Yu-Ling Zhang Zheng Qin Meng Xu qian qiao Sha Li Shan-Wei Li Yan Zhang State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai’anShandong 271018China College of Life Sciences Nankai UniversityTianjin 300071China

Cell polarity operates across a broad range of spatial and temporal scales and is essential for specific biological functions of polarized cells.Tip growth is a special type of polarization in which a single and unique polarization site is established and maintained,as for the growth of root hairs and pollen tubes in plants.Extensive studies in past decades have demonstrated that the spatiotemporal localization and activity of Rho of Plants(ROPs),the only class of Rho GTPases in plants,are critical for tip growth.ROPs are switched on or off by different factors to initiate dynamic intracellular activities,leading to tip growth.Recent studies have also uncovered several feedback modules for ROP signaling.In this review,we summarize recent progress on ROP signaling in tip growth,focusing on molecular mechanisms that underlie the dynamic distribution and activity of ROPs in Arabidopsis.We also highlight feedback modules that control ROPmediated tip growth and provide a perspective for building a complex ROP signaling network.Finally,we provide an evolutionary perspective for ROP-mediated tip growth in Physcomitrella patens and during plant–rhizobia interaction.

关键词： Rho GTPases feedback polarization root hair pollen tube

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基于图像处理和改进DenseNet网络的小黄鱼新鲜度识别

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南方水产科学 2024年第3期20卷 133-142页

作者：张洪交张存喜王瑞王可乔倩浙江海洋大学海洋工程装备学院浙江舟山316002 浙江海洋大学船舶与海运学院浙江舟山316002

传统水产品新鲜度检测方法存在对样本破坏较大、操作步骤繁琐、检测准确率及效率较低等一系列问题。针对上述问题,以小黄鱼(Larimichthys polyactis)新鲜度高效、准确识别为目标,提出了一种基于改进DenseNet网络的小黄鱼新鲜度识别模型... 详细信息

传统水产品新鲜度检测方法存在对样本破坏较大、操作步骤繁琐、检测准确率及效率较低等一系列问题。针对上述问题,以小黄鱼(Larimichthys polyactis)新鲜度高效、准确识别为目标,提出了一种基于改进DenseNet网络的小黄鱼新鲜度识别模型。首先,在DenseNet网络结构中的每个Denseblock模块引入SENet注意力机制模块,实现特征通道特征重标定,加强网络对当前有益特征的提取,摒弃无作用的特征。其次,改进卷积层的第一层,增加网络的非线性能力和特征表达能力。为防止训练过程中出现梯度消失的现象,用PReLU激活函数代替原网络的ReLU激活函数。最后,与原DenseNet网络模型及其他经典神经网络模型进行对比实验。结果表明,构建的基于迁移学习的FishNet模型在自建的小黄鱼新鲜度数据集上识别准确率达91.53%,模型具有较高的识别准确率和较强的鲁棒性,解决了水产品新鲜度检测高效和精准识别问题,也为开发智能新鲜度识别系统提供了参考。

关键词：小黄鱼新鲜度检测 DenseNet 注意力机制图像识别

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Oil Content and Nervonic Acid Content of Acer truncatum Seeds from 14 Regions in China

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Horticultural Plant Journal 2019年第1期5卷 24-30页

作者： qian qiao Xue Wang Hongjian Ren Kai An Zhen Feng Tiantian Cheng Zhongkui Sun College of Forestry Shandong Agricultural University Shandong Institute of Pomology Taishan Forestry Science Institute Tai'an Shidai Technology Development Limited Company

Acer truncatum is considered a promising species as a raw material to produce nervonic acid(NA). The goal of this study was to explore the variation of oil content and nervonic acid content of 138 accessions native to 14 regions in China as well as provide guidance for establishing plantations and improving the production of nervonic acid. A large range of variation was found in oil content, nervonic acid per gram of oil, and nervonic acid per gram of seed(17.81%–36.56%, 3.90%–7.85%, and 0.84%–2.31%, respectively). Accessions YS-6, ABZ-6 and DQTL-8 were found as the most promising potential oil sources; PQ-2 was considered the optimal germplasm, and JY-6, NJ-8, LF-6, and CC-8 were found as promising potential sources for producing NA. A very significant difference(P < 0.01) among 14 regions was observed. DQTL and YS regions are considered the optimal farming regions for crops with high oil content, while the CC and PQ regions are regarded as the most suitable regions for nervonic acid production. A geographical trend from South to North was observed in which amount of seed oil increased but no such trend in nervonic acid content was observed. The data collected in the study on oil content and nervonic acid in accessions of A. truncatum in various regions can be utilized for establishing plantations of promising genotypes through clonal means.

关键词： Acer truncatum Seed oil content Nervonic acid Variation

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星三角降压启动电气线路检测系统开发

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山西电子技术 2024年第1期 52-55页

作者：燕秀秀乔倩山西工程职业学院山西太原030009

设计了一套基于单片机STM32的星三角降压启动的智能电气线路检测系统,能够不带高电压实操,智能辅助纠错线路,使得电气设备在实操过程中几乎零损耗,且支持多次反复训练。智能电气线路检测系统是基于实际教学需求,在STM32作为主控芯片、... 详细信息

设计了一套基于单片机STM32的星三角降压启动的智能电气线路检测系统,能够不带高电压实操,智能辅助纠错线路,使得电气设备在实操过程中几乎零损耗,且支持多次反复训练。智能电气线路检测系统是基于实际教学需求,在STM32作为主控芯片、智能交互屏、继电器检测模块、电机控制模块等硬件基础上,应用C编程语言程序设计,并进行功能模块化开发设计。智能电气线路检测系统具有可多次重复使用且使用灵活的特点。

关键词：电气线路检测 STM32F103ZET6 继电器检测智能交互屏

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