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Fentanyl inhibits glucose-stimulated insulin release from β-cells in rat pancreatic islets

World Journal of Gastroenterology 2009年第33期15卷 4163-4169页

作者： Tao-lai qian Xin-Hua Wang Sheng Liu Liang Ma Ying Lu Department of Pain Management Shanghai East Hospital Tongji University Shanghai 200120 China Department of Anesthesia Shanghai East HospitalTongji University Shanghai 200120 China Cell Therapy Center Shanghai East Hospital Tongji University Shanghai 200120 China Central Laboratory Shanghai East Hospital Tongji University Shanghai 200120 China

AIM： TO explore the effects of fentanyl on insulin release from freshly isolated rat pancreatic islets in static culture. METHODS： Islets were isolated from the pancreas of mature Sprague Dawley rats by common bile duct intraductal collagenase V digestion and were purified by discontinuous Ficoll density gradient centrifugation. The islets were divided into four groups according to the fentanyl concentration： control group （0 ng/mL）, group I （0.3 ng/mL）, group I （3.0 ng/mL）, and group III （30 ng/mL）. In each group, the islets were co-cultured for 48 h with drugs under static conditions with fentanyl alone, fentanyl ＋ 0.1 μg/mL naloxone or fentanyl ＋ 1.0 μg/mL naloxone. Cell viability was assessed by the MTT assay. Insulin release in response to low and high concentrations （2.8 mmol/L and 16.7 mmol/L, respectively） of glucose was investigated and electron microscopy morphological assessment was performed. RESULTS： Low- and high-glucose-stimulated insulin release in the control group was significantly higher than in groups I and II （62.33 ± 9.67 μIU vs 47.75 ± 8.47 μIU, 39.67 ± 6.18 μIU and 125.5 ± 22.04 μIU vs 96.17 ± 14.17 μIU, 75.17 ± 13.57 μIU, respectively, P 〈 0.01） and was lowest in group III （P 〈 0.01）. After adding 1 μg/mL naloxone, insulin release in groups II and II was not different from the control group. Electron microscopy studies showed that the islets were damaged by 30 ng/ml fentanyl. CONCLUSION： Fentanyl inhibited glucose-stimulated insulin release from rat islets, which could be prevented by naloxone. Higher concentrations of fentanyl significantly damaged β-cells of rat islets.

关键词： Fentanyl Inhibition Insulin release Islets

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Managing immune checkpoint inhibitor-associated gastritis: Insights and strategies

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World Journal of Gastroenterology 2024年第24期30卷 3120-3122页

作者： Li-Li Yu Zhi-Lin He Xin-lai qian Department of Pathology The Third Affiliated Hospital of Xinxiang Medical UniversityXinxiang Medical UniversityXinxiang 453003Henan ProvinceChina Department of Basic Medical Sciences Xinxiang Medical UniversityXinxiang 453003Henan ProvinceChina

Immune checkpoint inhibitors(ICIs)are widely used due to their effectiveness in treating various ***-related adverse events(irAEs)are defined as adverse effects resulting from ICI *** irAEs are a common type of irAEs characterized by intestinal side effects,such as diarrhea and colitis,which may lead to the discontinuation of ICIs.

关键词： Immunotherapy Immune checkpoint inhibitor Immune checkpoint inhibitor-related gastritis Immune-related adverse events Autoimmune responses

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Ginkgo biloba extract improved cognitive and neurological functions of acute ischaemic stroke:a randomised controlled trial

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Stroke & Vascular Neurology 2017年第4期2卷 189-197页

作者： Shanshan Li Xinjiang Zhang Qi Fang Junshan Zhou Meijuan Zhang Hui Wang Yan Chen Biyun Xu Yanfeng Wu lai qian Yun Xu Department of Neurology Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingChina The State Key Laboratory of Pharmaceutical Biotechnology Nanjing UniversityNanjingChina Jiangsu Key Laboratory for Molecular Medicine Nanjing University Medical SchoolNanjingChina Nanjing Clinic Medicine Centre for Neurological and Psychiatric Diseases NanjingChina Department of Neurology Yangzhou No 1 People's HospitalYangzhouChina Department of Neurology The First Affiliated Hospital of Soochow UniversitySuzhouChina Department of Neurology The Affiliated Nanjing First Hospital of Nanjing Medical UniversityNanjingChina Department of Neurology The Second Affiliated Hospital of Nanjing Medical UniversityNanjingChina Departments of Analysis Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingChina

Purpose To evaluate the efficacy and safety of Ginkgo biloba extract(GBE)in acute ischaemic stroke and its impact on the recurrence of vascular *** We conducted a multicentre,prospective,randomised,open label,blinded,controlled clinical trial enrollingpatients with an onset of acute stroke within 7 days from five hospitals in China Jiangsu *** were assigned to the GBE group(450 mg GBE with 100 mg aspirin daily)or the control group(100 mg aspirin daily)for 6 *** primary outcome was the decline in the Montreal Cognitive Assessment score at 6 *** outcomes were other neuropsychological tests of cognitive and neurological function,the the incidence of adverse events and vascular *** 348 patients were enrolled:179 in the GBE group and 169 in the control *** 18 patients lost to follow-up,the dropout rate was 5.17%.Admission data between two groups were similar,but in the GBE group there was a marked slow down in the decline in the Montreal Cognitive Assessment scores(−2.77±0.21 vs−1.99±0.23,P=0.0116(30 days);−3.34±0.24 vs−2.48±0.26,P=0.0165(90 days);−4.00±0.26 vs−2.71±0.26,P=0.0004(180 days))compared with *** National Institutes of Health Stroke Scale scores at 12 and 30 days,the modified Rankin Scale scores for independent rate at 30,90 and 180 days,and the Barthel Index scores at 30,90 and 180 days in the GBE group were significantly improved compared with *** were also observedin GBE groups for Mini-Metal State Examination scores of 30,90 and 180 days,Webster's digit symbol test scores at 30 days and Executive Dysfunction Index scores at 30 and 180 *** significant differences were seen in the incidence of adverse events or vascular *** We conclude that GBE in combination with aspirin treatment alleviated cognitive and neurological deficits after acute ischaemic stroke without increasing the incidence of vascular events.

关键词： neurological random incidence

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Application of adaptive fuzzy control technology to pressure control of a pressurizer

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Journal of Marine Science and Application 2005年第1期4卷 39-43页

作者： YANGBen-kun BIANXin-qian GUOWei-lai SchoolofPowerandNuclearEngineering HarbinEngineeringUniversityHarbin150001China

A pressurizer is one of important equipment in a pressurized water reactor plant. It is used to maintain the pressure of primary coolant within allowed range because the sharp change of coolant pressure affects the security of reactor, therefor, the study of pressurizer’s pressure control methods is very important. In this paper, an adaptive fuzzy controller is presented for pressure control of a presurizer in a nuclear power plant. The controller can on-line tune fuzzy control rules and parameters by self-learning in the actual control process, which possesses the way of thinking like human to make a decision. The simulation results for a pressurized water reactor plant show that the adaptive fuzzy controller has optimum and intelligent characteristics, which prove the controller is effective.

关键词： fuzzy control pressurizer pressure control simulation

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Information tracing model based on PageRank

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网络与信息安全学报 2017年第8期3卷 68-76页

作者： LI qian lai Jia-wei XIAO Yun-peng WU Bin Beijing Key Laboratory of Intelligent Telecommunications Software and Multimedia Beijing University of Posts and Telecommunications Chongqing Engineering Laboratory of Network and Information Security Chongqing University of Posts and Telecommunications

In social network, original publisher and important nodes in the diffusion process can be traced by analyzing the spreading network of a hot topic. The participated users and spreading network structure of a hot topic build an information tracing model, which mines the source and important diffusion nodes. Firstly, it analyzed the development trend of a hot topic and extracts the users involved. Secondly, it established a user network according to the following relationship of the users involved. Thirdly, the contribution rate of users on the development of the hot topic was initialized, and the Page Rank algorithm was used to construct the information tracing model. Finally, the Top k users were selected as the information publisher and important users of the hot topic according to the contribution rate. Experimental results showed that our model can effectively discover the hot topic of the publisher and important users.

关键词：计算机网络网络管理应用程序信息安全

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RNA interference targeting mu-opioid receptors reverses the inhibition of fentanyl on glucose-evoked insulin release of rat islets

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Chinese Medical Journal 2010年第24期123卷 3652-3657页

作者： qian Tao-lai ZHANG Lei WANG Xin-hua LIU Sheng MA Liang LU Ying Department of Anesthesiology Shenzhen Shekou People's Hospital Shenzhen Guangdong 518067 China Pain Management Department Shanghai Dongfang Hospital Tongji University Shanghai 200120 China Department of Anesthesiology Shanghai Dongfang Hospital Tongji University Shanghai 200120 China Cell Therapy Center Shanghai Dongfang Hospital Tongji University Shanghai 200120 China Central Laboratory Shanghai Dongfang Hospital Tongji University Shanghai 200120 China

Background Mu opioid receptor plays an important role in many physiological functions. Fentanyl is a widely used opioid receptor agonist for analgesia. This study was conducted to test the role of mu-opioid receptor on insulin release by determining whether fentanyl affected insulin release from freshly isolated rat pancreatic islets and if small interfering RNAs （siRNA） targeting mu-opioid receptor in the islets could knock down mu-opioid receptor *** Islets were isolated from ripe SD rats＇ pancreas by common bile duct intraductal collagenase V digestion and purified by discontinuous Ficoll density gradient centrifugation. The siRNA knock-down of mu-opioid receptor mRNA and protein in islet cells was analyzed by semi-quantitative real time-PCR and Western blotting. After siRNA-transfection for 48 hours, the islets were co-cultured with fentanyl as follows： 0 ng/ml, 3 ng/ml and 30 ng/ml for 48 hours. Then glucose-evoked insulin release was performed. As a control, the insulin release was also analyzed in islets without siRNA-trasfection after being co-cultured with fentanyl for 48 *** After 48 hours of transfections, specific siRNA targeting of mu-opioid receptors produced significant reduction of mu-opioid receptor mRNA and protein （P ＜0.01）. Fentanyl significantly inhibited glucose-evoked insulin release in islets in a concentration dependent manner （P ＜0.01）. But after siRNA-transfection for 48 hours, the inhibition on glucose-evoked insulin reiease was reversed （P ＜0.01）.Conclusions RNA interference specifically reduces mu-opioid receptor mRNA and protein expression, leading to reversal of the fentanyl-induced inhibition on glucose-evoked insulin release of rat islets. The activation of opioid receptor induced by fentanyl functions to inhibit insulin release. The use of RNAi presents a promising tool for future research in diabetic mechanisms and a novel therapy for diabetes.

关键词： RNA interference mu-opioid receptor fentanyl inhibition insulin release

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Novel PIKfyve/Tubulin Dual-target Inhibitor as a Promising Therapeutic Strategy for B-cell Acute Lymphoblastic Leukemia

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Current Medical Science 2024年第2期44卷 298-308页

作者： Zhen LU qian lai Zhi-feng LI Meng-ya ZHONG Yue-long JIANG Li-ying FENG Jie ZHA Jing-wei YAO Yin LI Xian-ming DENG Bing XU Department of Hematology The First Affiliated Hospital of Xiamen University and Institute of HematologySchool of MedicineXiamen UniversityXiamen361005China Key Laboratory of Xiamen for Diagnosis and Treatment of Hematological Malignancy Xiamen361005China Department of Oncology The First Affiliated Hospital of Jinan UniversityJinan UniversityGuangzhou510630China State Key Laboratory of Cellular Stress Biology Innovation Center for Cell Signaling NetworkSchool of Life SciencesXiamen UniversityXiamen361003China

Objective:In B-cell acute lymphoblastic leukemia(B-ALL),current intensive chemotherapies for adult patients fail to achieve durable responses in more than 50%of cases,underscoring the urgent need for new therapeutic regimens for this patient *** present study aimed to determine whether HZX-02-059,a novel dual-target inhibitor targeting both phosphatidylinositol-3-phosphate 5-kinase(PIKfyve)and tubulin,is lethal to B-ALL cells and is a potential therapeutic for B-ALL ***:Cell proliferation,vacuolization,apoptosis,cell cycle,and in-vivo tumor growth were *** addition,Genome-wide RNA-sequencing studies were conducted to elucidate the mechanisms of action underlying the anti-leukemia activity of HZX-02-059 in ***:HZX-02-059 was found to inhibit cell proliferation,induce vacuolization,promote apoptosis,block the cell cycle,and reduce in-vivo tumor *** of the p53 pathway and suppression of the phosphoinositide 3-kinase(PI3K)/AKT pathway and the downstream transcription factors c-Myc and NF-κB were responsible for these ***:Overall,these findings suggest that HZX-02-059 is a promising agent for the treatment of B-ALL patients resistant to conventional therapies.

关键词： B-cell acute lymphoblastic leukemia dual-target inhibitor NF-kB c-Myc PI3K/AKT p53

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脑白质异常聋儿人工耳蜗植入后效果初步分析

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中华耳科学杂志 2016年第2期14卷 202-207页

作者：赖倩钟时勋重庆医科大学附属第一医院耳鼻咽喉科重庆400016

目的探讨对脑白质异常的重度感音神经性聋患儿,人工耳蜗植入手术的可行性及听力言语康复效果分析。方法自2012年1月至2014年12月行人工耳蜗植入术的120例(120耳)重度感音神经性聋患儿中,有脑白质异常的患儿15例。随机抽取15例脑白质无... 详细信息

目的探讨对脑白质异常的重度感音神经性聋患儿,人工耳蜗植入手术的可行性及听力言语康复效果分析。方法自2012年1月至2014年12月行人工耳蜗植入术的120例(120耳)重度感音神经性聋患儿中,有脑白质异常的患儿15例。随机抽取15例脑白质无异常的聋儿作为对照组,比较两组患儿植入人工耳蜗后手术效果、开机调试结果及听力言语康复效果。结果 130例(30耳)电极全部植入耳蜗内;2所有患者术后无脑膜炎、面瘫、脑脊液漏等并发症;3所有脑白质异常患儿在人工耳蜗植入术后都有听觉;4脑白质异常儿童术后平均听阈与对照组无明显区别;5随访6至24月后,脑白质异常患儿的听觉行为分级标准(categories of auditory performance,CAP)和言语可懂度分级标准(speech intelligibility rating,SIR)与对照组比较,差异均无显著性意义(P>0.05)。结论经过严格术前评估后,人工耳蜗植入可应用于脑白质异常伴重度感音神经性聋的患儿,其术后效果与脑白质无异常的聋儿植入效果基本一致,术后的听力言语康复效果无明显差异。

关键词：感音神经性聋人工耳蜗脑白质异常听觉和言语康复

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