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ALKBH5 gene polymorphisms and risk of neuroblastoma in Chinese children from Jiangsu Province

Cancer Innovation 2024年第2期3卷 72-79页

作者： qian guan Xinxin Zhang Jiabin Liu Chunlei Zhou Jinhong Zhu Haiyan Wu Zhenjian Zhuo Jing He Department of Pediatric Surgery Guangzhou Institute of PediatricsGuangdong Provincial Key Laboratory of Research in Structural Birth Defect DiseaseGuangdong Provincial Clinical Research Center for Child HealthGuangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhouGuangdongChina Department of Pathology Children's Hospital of Nanjing Medical UniversityNanjingJiangsuChina Department of Clinical Laboratory BiobankHarbin Medical University Cancer HospitalHarbinHeilongjiangChina Laboratory Animal Center School of Chemical Biology and BiotechnologyPeking University Shenzhen Graduate SchoolShenzhenGuangdongChina

Background:Neuroblastoma is one of the most common extracranial malignant solid tumors in children.AlkB homolog 5(ALKBH5)is an RNA N6-methyladenosine(m6A)demethylase that plays a critical role in tumorigenesis and development.We assessed the association between single nucleotide polymorphisms(SNPs)in ALKBH5 and the risk of neuroblastoma in a case-control study including 402 patients and 473 non-cancer controls.Methods:Genotyping was determined by the TaqMan method.The association between ALKBH5 polymorphisms(rs1378602 and rs8400)and the risk of neuroblastoma was evaluated using the odds ratio(OR)and 95%confidence interval(CI).Results:We found no strong association of ALKBH5 rs1378602 and rs8400 with neuroblastoma risk.Further stratification analysis by age,sex,primary site,and clinical stage showed that the rs1378602 AG/AA genotype was associated with a lower risk of neuroblastoma in males(adjusted OR=0.58,95%CI=0.35–0.97,p=0.036)and children with retroperitoneal neuroblastoma(adjusted OR=0.58,95%CI=0.34–0.98,p=0.040).Conclusions:ALKBH5 SNPs do not seem to be associated with neuroblastoma risk.More studies are required to confirm this negative result and reveal the relationship between gene polymorphisms of the m6A modifier ALKBH5 and neuroblastoma.

关键词： ALKBH5 neuroblastoma polymorphism susceptibility

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Research progress on insomnia treated by traditional Chinese medicine and acupuncture based on microbial-gut-brain axis theory

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World Journal of Clinical Cases 2024年第18期12卷 3314-3320页

作者： Li-Min Pan Zhi-Bo Hong Rui-qian guan Department of Outpatient Deputy Chief Physician The First Affiliated Hospital of Heilongjiang University of Chinese MedicineHaerbin 150010Heilongjiang ProvinceChina School of Heilongjiang University of Chinese Medicine Graduate Heilongjiang University of Chinese MedicineHaerbin 150000Heilongjiang ProvinceChina Heilongjiang University of Chinese Medicine The Second Affiliated Hospital of Heilongjiang University of Chinese MedicineHaerbin 150010Heilongjiang ProvinceChina

Insomnia,as one of the emotional diseases,has been increasing in recent years,which has a great impact on people's life and work.Therefore,researchers are eager to find a more perfect treatment.The microbiome-gut-brain axis is a new theory that has gradually become popular abroad in recent years and has a profound impact in the field of insomnia.In recent years,traditional Chinese medicine(TCM)has played an increasingly important role in the treatment of insomnia,especially acupuncture and Chinese herbal medicine.It is the main method of TCM in the treatment of insomnia.This paper mainly reviews the combination degree of"microorganism-gut-brain axis"theory with TCM and acupuncture under the system of TCM.To explore the mechanism of TCM and acupuncture in the treatment of insomnia under the guidance of"microorganismgut-brain axis"theory,in order to provide a new idea for the diagnosis and treatment of insomnia.

关键词： Insomnia Microorganism Intestine-brain axis Traditional Chinese medicine Acupuncture and moxibustion

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科学研究的数据生态及其模式演进研究

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科学学研究 2024年第4期42卷 673-682页

作者：夏义堃管茜南京大学数据管理创新研究中心江苏苏州215163 武汉大学信息管理学院湖北省武汉430072

数据是现代科学研究不可或缺的重要组成部分,数据生态及其模式演进对于促进科研创新、推动学术出版至关重要。基于概念回溯和比较分析,本文运用数据生态理论深入阐述了科研创新与学术出版的内在逻辑关联,搭建了以科研创新链-数据链-学... 详细信息

数据是现代科学研究不可或缺的重要组成部分,数据生态及其模式演进对于促进科研创新、推动学术出版至关重要。基于概念回溯和比较分析,本文运用数据生态理论深入阐述了科研创新与学术出版的内在逻辑关联,搭建了以科研创新链-数据链-学术出版链为核心的一体化分析框架。同时,结合不同研究范式的演进,分析其数据生态的主要表现,如不同科研范式下数据思维、数据制度、数据主体关系、数据管理结构、数据流通环境、数据管理方法等。最后,提出科研数据生态模式演进的理论模型,为加强数据驱动型研究范式下我国科研数据管理的深入推进提供新的思路与对策建议。

关键词：科研数据数据生态科研创新数据共享学术出版

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Applying RNA sequencing technology to explore repair mechanism of Tuina on gastrocnemius muscle in sciatic nerve injury rats

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Chinese Medical Journal 2022年第19期135卷 2378-2379页

作者： Taotao Lyu Zhifeng Liu Tianyuan Yu Mengqian Lu Yingqi Zhang Yi Jiao Hourong Wang Yajing Xu qian guan School of Acupuncture Moxibustion and TuinaBejing University of Chinese MedicineBeijing 100029China Tuina Research Center of Beijing University of Chinese Medicine Bejing 100029China

To the Editor:Peripheral nerve injury(PNI)refers to the injury of the peripheral nerve plexus,nerve trunk,or its branches undertheaction of externalforces.Skeletal muscle-derived cells servea strong secretory,function while they can differentiate into Schwann cell-like cells or other ceil types,so that it can,effectively promote regeneration'of peripheral nerves.llUsing some specific acupuncture points with mild mechanical stimulation on the surface can have a positive effect on nerve repair.2i Tuina helps relieve symptoms such as muscle spasms and pain caused by nerve damage.There are three points belonging to the Bladder meridian of foot Taiyang(BL)and the Gallbladder meridian of foot Shaoyang(GB),which usually are selected to carry out Tuina.According to the anatomical nerve position,Yinmen(BL37)is on the sciatic nerve trunk,Chengshan(BL57)is on the tibial nerve,and Yanglingquan(GB34)is on the common peroneal nerve;meanwhile,the three points are on the sciatic nerve or its branches.The three points are on the biceps femoris,gastrocnemius,and tibial anterior muscles.

关键词： meridian gastro acupuncture

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An investigation of the influence of intestinal flora in external traditional Chinese medicine therapy

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Journal of Traditional Chinese Medical Sciences 2023年第1期10卷 10-18页

作者： Hourong Wang Zhifeng Liu Tianyuan Yu Yingqi Zhang Yi Jiao Di Liu Yajing Xu qian guan Mengqian Lu School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese MedicineBeijing 102488China Dongzhimen Hospital Beijing University of Chinese MedicineBeijing 100700China Dongfang Hospital Beijing University of Chinese MedicineBeijing 100078China

External therapy,which has been used for millennia to treat disease,has now become the newest member of complementary and alternative therapies.A growing number of clinical studies and animal experiments have shown that external therapy exerts a positive effect on the intestinal flora.In this paper,we review the research related to external therapy on various systemic diseases and focus on changes in the intestinal flora.We found that studies on external therapy are dominated by acupuncture and tuina,primarily aimed at the treatment of digestive and neurological diseases.However,the deeper mechanism of regulation of the intestinal flora by external therapy has not yet been experimentally confirmed.

关键词： Traditional Chinese medicine External therapy Intestinal flora Digestive diseases Neurological diseases Acupoint Mechanism

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Effect of pediatric tuina on hypothalamic metabolites in young rabbits using liquid chromatography-mass spectrometry

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Journal of Traditional Chinese Medical Sciences 2022年第2期9卷 188-196页

作者： Zhifeng Liu Yi Jiao Tianyuan Yu Yingqi Zhang Di Liu Hourong Wang Yajing Xu qian guan Taotao Lv Jian Shu School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese MedicineBeijing102488China Department of Acupuncture Dongfang Hospital Beijing University of Chinese MedicineBeijing100078China Shu Acupuncture Clinic FayettevilleNY13066USA

Objective: To investigate the changes in the hypothalamic metabolites of lipopolysaccharide(LPS) febrile young rabbits after the treatment with pediatric tuina.Methods: A total of 30 young rabbits were randomly assigned into three groups: the normal group, the model group, and the tuina group. Both the model group and the tuina group were injected intravenously with LPS. “Six antipyretic manipulations”(pushing Tianmen, pushing Kangong, kneading Taiyang,kneading Erhougaogu, clearing Tianheshui, and pushing Jizhu) were administered 1 h after the LPS injection in the tuina group. The rectal temperatures of the young rabbits were monitored during the experiment to explore the antipyretic effect. Three hours after the injection, the content of interleukin-1β(IL-1β), tumor necrosis factor(TNF)-a, and prostaglandin E;(PGE;) in the serum was detected. In addition, liquid chromatography-mass spectrometry(LC-MS) was used for the hypothalamus metabolomics.Results: Compared with the model group, the rectal temperature of the tuina group was decreased at 2 h and 3 h after the LPS injection(P =.04, P =.03, respectively), and the content of IL-1β, TNF-a, and PGE2was decreased(P =.03, P =.003, and P =.008, respectively). The metabolomics results showed that there were 23 potential biomarkers after the tuina intervention, enriching 27 pathways. Lipid metabolites,especially glycerophospholipids, were a majority of the altered metabolites. The primary metabolic pathways affected by tuina included the arachidonic acid metabolism, the GABAergic synapse, D-glutamine and D-glutamate metabolism, and the glutamatergic synapse.Conclusion: Pediatric tuina reduced the temperature of the febrile rabbits and downregulated the expression of IL-1β, TNF-a, and PGE2, and the antipyretic mechanism may be related to changes in metabolites and metabolic pathways in the hypothalamus.

关键词： Fever Pediatric tuina Metabolomics Liquid chromatography coupled with mass spectrometry Hypothalamus Interleukin-1b Tumor necrosis factor-a Prostaglandin E_(2)

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Variant rs8400 enhances ALKBH5 expression through disrupting miR-186 binding and promotes neuroblastoma progression

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Chinese Journal of Cancer Research 2023年第2期35卷 140-162页

作者： qian guan Huiran Lin Wenfeng Hua Lei Lin Jiabin Liu Linqing Deng Jiao Zhang Jiwen Cheng Zhonghua Yang Yong Li Jun Bian Haixia Zhou Suhong Li Li Li Lei Miao Huimin Xia Jing He Zhenjian Zhuo School of Medicine South China University of TechnologyGuangzhou 510006China Department of Pediatric Surgery Guangzhou Institute of PediatricsGuangdong Provincial Key Laboratory of Research in Structural Birth Defect DiseaseGuangdong Provincial Clinical Research Center for Child HealthGuangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Faculty of Medicine Macao University of Science and TechnologyMacao 99078China Research Institute for Maternal and Child Health Guangdong Second Provincial General HospitalGuangzhou 510317China Department of Pediatric Surgery the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of Pediatric Surgery the Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710004China Department of Pediatric Surgery Shengjing Hospital of China Medical UniversityShenyang 110004China Department of Pediatric Surgery Hunan Children's HospitalChangsha 410004China Department of General Surgery Xi'an Children's HospitalXi'an Jiaotong University Affliated Children's HospitalXi'an 710003China Department of Hematology The Second Affliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhou 325027China Department of Pathology Children Hospital and Women Health Center of ShanxiTaiyuan 030013China Kunming Key Laboratory of Children Infection and Immunity Yunnan Key Laboratory of Children's Major Disease ResearchYunnan Institute of Pediatrics ResearchYunnan Medical Center for Pediatric DiseasesKunming Children's HospitalKunming 650228China Laboratory Animal Center School of Chemical Biology and BiotechnologyPeking University Shenzhen Graduate SchoolShenzhen 518055China

Objective:AlkB homolog 5(ALKBH5)has been proven to be closely related to tumors.However,the role and molecular mechanism of ALKBH5 in neuroblastomas have rarely been reported.Methods:The potential functional single-nucleotide polymorphisms(SNPs)in ALKBH5 were identified by National Center for Biotechnology Information(NCBI)dbSNP screening and SNPinfo software.TaqMan probes were used for genotyping.A multiple logistic regression model was used to evaluate the effects of different SNP loci on the risk of neuroblastoma.The expression of ALKBH5 in neuroblastoma was evaluated by Western blotting and immunohistochemistry(IHC).Cell counting kit-8(CCK-8),plate colony formation and 5-ethynyl-2'-deoxyuridine(EdU)incorporation assays were used to evaluate cell proliferation.Wound healing and Transwell assays were used to compare cell migration and invasion.Thermodynamic modelling was performed to predict the ability of miRNAs to bind to ALKBH5 with the rs8400 G/A polymorphism.RNA sequencing,N6-methyladenosine(mA)sequencing,mA methylated RNA immunoprecipitation(MeRIP)and a luciferase assay were used to identify the targeting effect of ALKBH5 on SPP1.Results:ALKBH5 was highly expressed in neuroblastoma.Knocking down ALKBH5 inhibited the proliferation,migration and invasion of cancer cells.miR-186-3p negatively regulates the expression of ALKBH5,and this ability is affected by the rs8400 polymorphism.When the G nucleotide was mutated to A,the ability of miR-186-3p to bind to the 3'-UTR of ALKBH5 decreased,resulting in upregulation of ALKBH5.SPPI is the downstream target gene of the ALKBH5 oncogene.Knocking down SPP1 partially restored the inhibitory effect of ALKBH5 downregulation on neuroblastoma.Downregulation of ALKBH5 can improve the therapeutic efficacy of carboplatin and etoposide in neuroblastoma.Conclusions:We first found that the rs8400 G>A polymorphism in the m6A demethylase-encoding gene ALKBH5 increases neuroblastoma susceptibility and determines the related mechanisms.The aberrant regulation of ALKBH5 by miR-186-3

关键词： Neuroblastoma risk ALKBH5 polymorphism progression

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不同转速对采煤机截割滚筒受力的影响研究

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机械管理开发 2024年第6期39卷 104-106页

作者：管乾晋能控股集团有限公司山西太原038000

利用多用途离散元素法建模软件EDEM构建有限元模型,模拟分析了不同转速下采煤机截割滚筒的受力情况。以MG500/1180-WD型采煤机为研究对象,探讨了转速变化对滚筒截割能力、受力分布的影响。研究发现,在滚筒转速一定的情况下,滚筒各方向... 详细信息

利用多用途离散元素法建模软件EDEM构建有限元模型,模拟分析了不同转速下采煤机截割滚筒的受力情况。以MG500/1180-WD型采煤机为研究对象,探讨了转速变化对滚筒截割能力、受力分布的影响。研究发现,在滚筒转速一定的情况下,滚筒各方向的受力呈现先增大后减小的趋势,转速为30r/min时滚筒受力最大。随着转速的增加,剩余黏结键数量逐渐降低,破煤率相应提高。从截割滚筒受力和破煤率层面出发,可以适当提升滚筒转速,但同时要考虑提高转速引发的其他问题。该研究为采煤机的优化设计、合理使用提供了理论支撑和实践指导。

关键词：滚筒转速采煤机滚筒受力 EDEM软件

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基于ABAQUS软件的采煤机行走轮应力分析

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机械管理开发 2024年第5期39卷 117-118,126页

作者：管乾晋能控股集团有限公司山西太原030000

介绍了一种四行走轮采煤机行走轮机构设计方案,并以此为基础,通过ABAQUS进行仿真模型构建,通过有限元分析方法,从弯曲应力和接触应力两个角度验证四行走轮采煤机行走轮机构运行时行走轮应力分布特点,初步确认设计方案的有效性。同时,为... 详细信息

介绍了一种四行走轮采煤机行走轮机构设计方案,并以此为基础,通过ABAQUS进行仿真模型构建,通过有限元分析方法,从弯曲应力和接触应力两个角度验证四行走轮采煤机行走轮机构运行时行走轮应力分布特点,初步确认设计方案的有效性。同时,为进一步确认四行走轮采煤机行走轮机构设计方案的应用成效,研究中还会将设计方案应用于工程实践。

关键词：采煤机行走轮应力分析 ABAQUS

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遥感找矿技术在地质矿产勘探中的应用探究

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世界有色金属 2024年第7期 79-81页

作者：陈晨宫贯乾华北地质勘查局第四地质大队河北秦皇岛066000

随着时代的发展,科技的不断进步,当前地质工作中对勘探环节的技术方法也有了一些变化。遥感找矿技术作为一种新兴的、先进的、智能的技术,将其应用于地质勘查中,能够有效地提升地质勘查的总体工作质量,并能有效地保护施工人员的生命安... 详细信息

随着时代的发展,科技的不断进步,当前地质工作中对勘探环节的技术方法也有了一些变化。遥感找矿技术作为一种新兴的、先进的、智能的技术,将其应用于地质勘查中,能够有效地提升地质勘查的总体工作质量,并能有效地保护施工人员的生命安全。本文根据目前的情况,探讨了利用遥感找矿技术开展地质勘探的方法。

关键词：遥感找矿技术地质矿产勘探策略

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