检索结果-南通市图书馆

Deciphering the regulatory and catalytic mechanisms of an unusual SAM-dependent enzyme

Signal Transduction and Targeted Therapy 2019年第1期4卷 530-537页

作者： qiu Sun Yuehong hu Yijun Gu Jiangkun huang Jun He Lan Luo Yi Yang Shuo Yin Chao Dou Tianqi Wang Xianghui Fu Ling He Shiqian qi Xiaofeng Zhu Shengyong Yang Xiawei Wei Wei Cheng Division of Respiratory and Critical Care Medicine Center of Infectious DiseasesNational Clinical Research Center for Geriatrics and State Key Laboratory of BiotherapyWest China Hospital of Sichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041China Shanghai Synchrotron Radiation Facility Zhangjiang LabZhangheng Road 239Pudong DistrictShanghai 201203China Department of Medicinal Chemistry Key Laboratory of Drug-Targeting and Drug Delivery System of the Education MinistrySichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial TechnologyWest China School of PharmacySichuan UniversityChengdu 610041China West China School of Public Health Sichuan UniversityChengdu 610041China

S-adenosyl-1-methionine(SAM)-dependent enzymes regulate various disease-related behaviors in all ***,the leporin biosynthesis enzyme LepI,a SAM-dependent enzyme,was reported to catalyze pericyclic reactions in leporin biosynthesis;however,the mechanisms underlying LepI activation and catalysis remain *** study aimed to investigate the molecular mechanisms of ***,we reported crystal structures of LepI bound to SAM/5′-deoxy-5′-(methylthio)adenosine(MTA),S-adenosyl-homocysteine(SAH),and SAM/substrate *** and biochemical analysis revealed that MTA or SAH inhibited the enzyme activities,whereas SAM activated the *** analysis of the substrate-bound structure of LepI demonstrated that this enzymatic retro-Claisen rearrangement was primarily driven by three critical polar residues His133,Arg197,Arg295 around the active site and assisted by SAM with unclear *** present studies indicate that the unique mechanisms underlying regulatory and catalysis of the unusual SAM-dependent enzyme LepI,not only strengthening current understanding of the fundamentally biochemical catalysis,but also providing novel insights into the design of SAM-dependent enzyme-specific small molecules.

关键词： mechanism. catalysis unusual

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Taxonomic notes of theridiid spiders (Araneae: Theridiidae) from China and Vietnam

引用

Zoological Research(Diversity and Conservation) 2024年第2期1卷 141-168页

作者： Ye-Jie Lin Chang-hao hu Dinh-Sac Pham Shu-qiang Li Institute of Zoology Chinese Academy of SciencesBeijing 100101China The State Key Laboratory of Biocatalysis and Enzyme Engineering of China College of Life ScienceHubei UniversityWuhanHubei 430062China Vietnam National Museum of Nature(VNMN) Vietnam Academy of Science and Technology(VAST)18 Hoang Quoc VietCau GiayHanoi 11306Vietnam

Ten new species of the spider family Theridiidae Sundevall,1833 from China and Vietnam are described:Chrysso shantinggui ***.(♂♀),Gushangzao andaoquan ***.(♂),Moneta oupeng ***.(♂♀),Phycosoma yanshun ***.(♂♀),Spinembolia gaofani ***.(♀),*** ***.(♂♀),*** ***.(♂♀),Stemmops dengfei ***.(♂♀),Takayus xiaorang ***.(♂♀),Yaginumena weidingguo ***.(♀).The unknown sexes of Allothymoites sculptilis Gao&Li,2014,Gyro zeppeli Lin&Li,2024,Parasteatoda longiducta Zhu,1998 are described for the first time.

关键词： Diagnosis New combination New species Asia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

公共图书馆未成年人设施安全标准的若干思考

引用

图书馆 2015年第6期 99-102页

作者：陈琦胡芳杭州图书馆浙江杭州310016 杭州少年儿童图书馆浙江杭州310007

通过对国内公共图书馆未成年人设施的安全现状进行分析,从建筑安全、服务设施安全、环境安全等方面探讨如何通过标准化建设来改善我国公共图书馆未成年人设施的安全现状,使公共图书馆真正成为一个安全的场所。

关键词：公共图书馆未成年人设施安全标准

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于加权模板转换的复杂山区铁路站址寻优

引用

西南交通大学学报 2017年第5期52卷 877-885页

作者：蒲浩张洪李伟胡建平胡光常中南大学土木工程学院湖南长沙410075 高速铁路建造技术国家工程实验室湖南长沙410075 中铁二院工程集团有限责任公司四川成都610031

为解决无站址备择情况下山区铁路车站自动选址问题,基于线站耦合约束要求及灰色关联分析理论,提出了"加权模板转换方法".该方法首先将铁路车站站坪区域范围简化为一个给定长、宽的空间矩形模板,并通过移动、旋转该模板搜索车站站心位置... 详细信息

为解决无站址备择情况下山区铁路车站自动选址问题,基于线站耦合约束要求及灰色关联分析理论,提出了"加权模板转换方法".该方法首先将铁路车站站坪区域范围简化为一个给定长、宽的空间矩形模板,并通过移动、旋转该模板搜索车站站心位置(矩形模板中心)和站坪方向(矩形模板纵向中心线方向);然后建立以用地代价、土石方工程代价、与居民区距离、线站耦合程度为评价指标的评价体系,并基于灰色关联分析理论,计算各项评价指标权重;最后计算通过移动、旋转得到站址方案的综合代价,经排序比选实现连续空间内铁路站址的自动寻优.以我国西南山区某铁路为例对该方法进行了验证,验证案例共输出49个站址方案,并依据综合设站代价由小到大进行了排序,结果表明,该方法可在复杂山区环境下自动生成铁路站址方案,辅助设计人员进行车站选址设计.

关键词：山岭地区铁路车站选址加权模板转换关联分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expression patterns of plasma von Willebrand factor and serum interleukin-8 in patients with early-stage severe pulmonary contusion

引用

World Journal of Emergency Medicine 2011年第2期2卷 122-126页

作者： Jin-xian qian Shi-qi Lu Yi-ming Zhao Jun-hao Lu ICU the First Affiliated Hospital of Soochow University Soochow 215006 China Blood lnstitute Soochow University Soochow China Wujiang Affiliated Hospital Nantong University Wujiang 215200 China

BACKGROUND： von Willebrand factor （vWF） is only released from endothelial cells and platelets and is an in vivo and in vitro marker of endothelial injury in septic patients with acute lung injury （ALI）. Interleukin-8 （IL-8）, as a proinflammatory mediator causing recruitment of inflammatory cells, induces an increase in oxidant stress mediators and makes it as a key parameter for localized inflammation. However, it has not been well established whether the level of serum IL-8 is associated with the severity of lung injury and whether it is a prognosis marker for severe lung contusion. This study was to investigate the expression of plasma vWF and IL-8 and their association with the severity and outcomes of severe pulmonary ***： A total of 63 patients were divided into a severe pulmonary contusion with acute respiratory distress syndrome （ARDS） group and a non-ARDS group, or a survivor group and a non- survivor group, or an injury severity score （ISS）〈20 group and an ISS 〉20 group. Another 20 healthy volunteers served as controls. The levels of plasma vWF and serum IL-8 were measured by enzyme- linked immunosorbent assay （ELISA） at 1,3, 5 and 7 days after injury. The expression patterns of the plasma vWF and serum IL-8 were compared between different groups. RESULTS： The concentrations of plasma vWF and serum IL-8 were significantly increased in all severe pulmonary contusion patients at all time points in comparison with the control group. The concentrations of plasma vWF in patients with ARDS increased during the whole study period, but vWF in patients with non-ARDS increased gradually until day 5 and then decreased at day 7. The concentration of serum IL-8 showed a similar expression pattern in both groups, but the expression increased more significantly in the ARDS group than in the non-ARDS group. Interestingly, both plasma vWF and serum IL-8 levels steadily increased in the non-survivor group. Furthermore, the level of plasma vWF was higher in th

关键词： Von Willebrand factor Interleukin-8 Pulmonary contusion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Unexpected right-sided sigmoid colon in laparoscopy: A case report and review of literature

引用

World Journal of Gastrointestinal Surgery 2024年第11期16卷 3606-3613页

作者： Shi-Fu hu Xiang-Yu Liu Han-Bo Liu Yuan-Yuan hao Department of General Surgery Tianjin Xiqing HospitalTianjin 300100China Department of Geriatrics Tianjin Xiqing HospitalTianjin 300100China

BACKGROUND The presence of a right-sided sigmoid colon is a rare anatomical variation usually discovered incidentally during surgical *** case report details an unexpected right-sided sigmoid colon identified during a laparoscopic appendectomy and examines the pertinent literature to explore its clinical *** SUMMARY A 71-year-old woman presented with acute appendicitis.A preoperative computed tomography(CT)scan showed peri-appendiceal inflammation but no significant anatomical *** laparoscopic surgery,an unexpected finding was encountered:The sigmoid colon was situated on the right side and exhibited an abnormal relationship with the cecum and ascending *** pathological examination confirmed appendicitis with no additional pathological *** right-sided sigmoid colon anomaly was verified through intraoperative assessment and later re-evaluation with CT and colonographic *** patient underwent a laparoscopic appendectomy and experienced a smooth postoperative *** This case highlights the necessity of being attentive to anatomical variations during laparoscopic surgery,particularly when managing appendicitis.A review of the literature indicated that the occurrence of a right-sided sigmoid colon is infrequent and may be associated with anomalies in midgut rotation during embryonic *** of this variation can help prevent surgical complications and inform future clinical practice.

关键词： Right-sided sigmoid colon Laparoscopy Anatomical variation Literature review Surgical implications Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Kinetic Characteristics of Neutralizing Antibody Responses Vary among Patients with COVID-19

引用

Biomedical and Environmental Sciences 2021年第12期34卷 976-983页

作者： LI Ling hua TU Hong Wei LIANG Dan WEN Chun Yan LI An An LIN Wei Yin hu Ke qi HONG Wen Shan LI Yue Ping SU Juan Zhao San Tao LI Wei YUAN Run Yu ZHOU Ping Ping hu Feng Yu TANG Xiao Ping KE Chang Wen KE Bi Xia CAI Wei Ping Guangzhou Eighth People’s Hospital Guangzhou Medical UniversityGuangzhou 510060GuangdongChina Guangdong Provincial Center for Diseases Control and Prevention Guangzhou 511430GuangdongChina School of Public Health Southern Medical UniversityGuangzhou 510515GuangdongChina Shantou University Medical College Shantou 515063GuangdongChina

Objective The coronavirus disease 2019(COVID-19) pandemic continues to present a major challenge to public health. Vaccine development requires an understanding of the kinetics of neutralizing antibody(NAb) responses to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Methods In total, 605 serum samples from 125 COVID-19 patients(from January 1 to March 14, 2020)varying in age, sex, severity of symptoms, and presence of underlying diseases were collected, and antibody titers were measured using a micro-neutralization assay with wild-type *** NAbs were detectable approximately 10 days post-onset(dpo) of symptoms and peaked at approximately 20 dpo. The NAb levels were slightly higher in young males and severe cases, while no significant difference was observed for the other classifications. In follow-up cases, the NAb titer had increased or stabilized in 18 cases, whereas it had decreased in 26 cases, and in one case NAbs were undetectable at the end of our observation. Although a decreasing trend in NAb titer was observed in many cases, the NAb level was generally still *** We demonstrated that NAb levels vary among all categories of COVID-19 patients. Longterm studies are needed to determine the longevity and protective efficiency of NAbs induced by SARS-CoV-2.

关键词： Sex Kinetics Neutralizing antibody SARS-CoV-2

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effectiveness of machine learning at modeling the relationship between Hi-C data and copy number variation

引用

Quantitative Biology 2024年第3期12卷 231-244页

作者： Yuyang Wang Yu Sun Zeyu Liu Bijia Chen Hebing Chen Chao Ren Xuanwei Lin Pengzhen hu Peiheng Jia Xiang Xu Kang Xu Ximeng Liu hao Li Xiaochen Bo Institute of Health Service and Transfusion Medicine BeijingChina College of Computer and Data Science Fuzhou UniversityFuzhouChina Beijing Institute of Radiation Medicine BeijingChina School of Life Sciences Northwestern Polytechnical UniversityXi’anChina School of Mathematics and Computer Science Shanxi Normal UniversityTaiyuanChina School of Software Shandong UniversityQingdaoChina

Copy number variation(CNV)refers to the number of copies of a specific sequence in a genome and is a type of chromatin structural *** development of the Hi-C technique has empowered research on the spatial structure of chromatins by capturing interactions between DNA *** utilized machine-learning methods including the linear transformation model and graph convolutional network(GCN)to detect CNV events from Hi-C data and reveal how CNV is related to three-dimensional interactions between genomic fragments in terms of the one-dimensional read count signal and features of the chromatin *** experimental results demonstrated a specific linear relation between the Hi-C read count and CNV for each chromosome that can be well qualified by the linear transformation *** addition,the GCN-based model could accurately extract features of the spatial structure from Hi-C data and infer the corresponding CNV across different chromosomes in a cancer cell *** performed a series of experiments including dimension reduction,transfer learning,and Hi-C data perturbation to comprehensively evaluate the utility and robustness of the GCN-based *** work can provide a benchmark for using machine learning to infer CNV from Hi-C data and serves as a necessary foundation for deeper understanding of the relationship between Hi-C data and CNV.

关键词： copy number variant deep learning graph convolution network Hi-C

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Experience of Objective Monitoring Management of Nosocomial Infection in ICU Ward

引用

麻醉与监护论坛 2013年第1期20卷 43-44页

作者： Jun Liu Jiang hao Ge-yuan Bian Ji-shen Luo qi Weng Department of Emergency ICU Kunming General Hospital of Chinese People's Liberation Army 650032

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

De novo mutation of NAXE(APOAIBP)-related early-onset progressive encephalopathy with brain edema and/or leukoencephalopathy-1:A case report

引用

World Journal of Clinical Cases 2023年第14期11卷 3340-3350页

作者： Le Ding Ting-Ting huang Guo-huan Ying Shang-Yu Wang Hai-Feng Xu hao qian Faiza Rahman Xiao-Peng Lu hu Guo Guo Zheng Gang Zhang Department of Neurology Children’s Hospital of Nanjing Medical UniversityNanjing 210008Jiangsu ProvinceChina Rehman Medical Institute Peshawar Peshawar 39250Pakistan

BACKGROUND Early-onset progressive encephalopathy with brain edema and/or leukoencephalopathy-1(PEBEL1)is a rare autosomal recessive severe neurometabolic *** aim of this study was to investigate the clinical characteristics and genetic pathogenicity of PEBEL1 caused by rare NAXE(or APOA1BP)-related *** SUMMARY The patient was a girl aged 2 years and 10 *** was hospitalized due to walking disorder for>40 *** clinical manifestations were ataxia,motor function regression,hypotonia,and eyelid *** 1 mo of hospitalization,she developed sigh breathing,respiratory failure,cerebellar edema and brain hernia,and finally she *** were found in cranial imaging,including cerebellar edema accompanied by symmetrical *** whole exome sequencing,we detected NAXE compound heterozygous variation(NM 144772.3)c.733A>C(***245Gln,dbSNP:rs770023429)and novel variation c.370G>T(***124Cys)in the germline *** clinical features and core phenotypes of this case were consistent with 18 previously reported cases of *** This is the first case of NAXE-related PEBEL1 with severe clinical phenotype in China' *** ***124Cys mutation discovered in this case has enriched the pathogenic variation spectrum of NAXE.

关键词： Encephalopathy Respiratory insufficiency Cerebral edema NAXE gene APOAIBP gene Novel variation Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：