检索结果-南通市图书馆

Single-cell omics: experimental workflow, data analyses and applications

Science China Life Sciences 2025年第1期68卷 5-102页

作者： fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao qinqin Wang Xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Recent Advances in Fibrous Materials for Hydroelectricity Generation

引用

Nano-Micro Letters 2025年第2期17卷 109-133页

作者： Can Ge Duo Xu Xiao feng xing Yang Zheheng Song Yuhang Song Jingyu Chen Yingcun Liu Chong Gao Yong Du Zhe Sun Weilin Xu Jian Fang College of Textile and Clothing Engineering Soochow UniversitySuzhou 215123People’s Republic of China National Engineering Laboratory for Modern Silk Soochow UniversitySuzhou 215123People’s Republic of China State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan 430200People’s Republic of China Institute of Functional Nano&Soft Materials(FUNSOM) Soochow UniversitySuzhou 215123People’s Republic of China Department of Electrical and Computer Engineering University of MassachusettsAmherstMA 01003USA Department of Materials Imperial College LondonSouth Kensington CampusLondon SW72AZUK College of Textile Science and Engineering Zhejiang Sci-Tech UniversityHangzhou 310018People’s Republic of China School of Materials Science and Engineering Shanghai Institute of Technology100 Haiquan RoadShanghai 201418People’s Republic of China

Depleting fossil energy sources and conventional polluting power generation pose a threat to sustainable *** generation from ubiquitous and spontaneous phase transitions between liquid and gaseous water has been considered a promising strategy for mitigating the energy *** materials with unique flexibility,processability,multifunctionality,and practicability have been widely applied for fibrous materials-based hydroelectricity generation(FHG).In this review,the power generation mechanisms,design principles,and electricity enhancement factors of FHG are first ***,the fabrication strategies and characteristics of varied constructions including 1D fiber,1D yarn,2D fabric,2D membrane,3D fibrous framework,and 3D fibrous gel are ***,the advanced functions of FHG during water harvesting,proton dissociation,ion separation,and charge accumulation processes are analyzed in ***,the potential applications including power supply,energy storage,electrical sensor,and information expression are also ***,some existing challenges are considered and prospects for future development are sincerely proposed.

关键词： Hydroelectricity Fibrous material Streaming potential Ion diffusion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

椎管内原发Rosai-Dorfman病的MRI表现(附4例报告)

引用

磁共振成像 2015年第3期6卷 204-207页

作者：韩壮马军肖伏龙冯奇星石家庄市第一医院磁共振室石家庄050021 首都医科大学附属北京天坛医院放射科北京100050

目的总结椎管内Rosai-Dorfman病(RDD)的MRI影像特点,增加对该病的认识,提高鉴别诊断能力。材料与方法回顾性分析4例病理确诊的椎管内RDD患者的MRI特点。结果 4例患者行椎管MRI平扫加增强扫描;2例患者位于颈椎管内,1例患者位于胸椎管内、... 详细信息

目的总结椎管内Rosai-Dorfman病(RDD)的MRI影像特点,增加对该病的认识,提高鉴别诊断能力。材料与方法回顾性分析4例病理确诊的椎管内RDD患者的MRI特点。结果 4例患者行椎管MRI平扫加增强扫描;2例患者位于颈椎管内,1例患者位于胸椎管内、1例位于骶管内。4例患者MRI显示椎管内髓外硬膜下占位,与脊膜关系密切,呈等T1、等T2信号,其中3例明显较均匀弥漫强化,1例呈较弱强化;强化程度弱于相邻脊膜。1例颈椎管患者为多发病灶。结论 MRI检查发现椎管内髓外硬膜下与脊膜关系密切的单发或者多发病灶,应考虑到椎管内RDD的可能,加扫磁共振波谱有助于鉴别。

关键词：组织细胞增多症,窦椎管磁共振成像

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

矿井提升系统常见故障类型分析与诊断技术研究

引用

煤矿现代化 2025年第1期34卷 148-152页

作者：邢琪晋能控股煤业集团同忻煤矿山西有限公司山西大同037000

针对矿井提升系统故障形式复杂、故障类型多样、传统故障诊断结果不准确等问题,本文对矿井提升系统常见故障类型和特征进行分析,指出了矿井提升机常见电动机故障、齿轮箱故障和减速机故障的原因和故障主要特征,确定了不同故障异常特征... 详细信息

针对矿井提升系统故障形式复杂、故障类型多样、传统故障诊断结果不准确等问题,本文对矿井提升系统常见故障类型和特征进行分析,指出了矿井提升机常见电动机故障、齿轮箱故障和减速机故障的原因和故障主要特征,确定了不同故障异常特征检测点的位置。建立了矿井提升机多维数据融合故障智能诊断系统,以现场实际应用的立式缠绕式双滚筒提升系统进行案例分析,在对应的部位安装传感器采集异常特征,对异常进行可视化分析,不同的故障类型得到振动曲线不同,通过对异常振动曲线可以判断提升系统故障类型。现场试验表明采集到的异常分析过程仅用时1.43 s,异常识别准确率高达98.4%,取得了满意的试验结果.

关键词：矿井提升系统可视化故障诊断远程运维

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

菊形团形成性胶质神经元肿瘤的MRI表现

引用

磁共振成像 2015年第4期6卷 241-245页

作者：冯奇星戴建平马军闵祥德首都医科大学附属北京天坛医院神经影像中心北京100050 华中科技大学同济医学院附属同济医院放射科武汉430030

目的：探讨菊形团形成性胶质神经元肿瘤（RGNT）的影像学特征，以提高对本病的认识。材料与方法回顾性分析10例（男∶女=3∶7，年龄介于8～45岁）经手术病理证实的RGNT患者的影像学资料。10例行MR平扫及增强扫描，其中3例行CT平扫。结... 详细信息

目的：探讨菊形团形成性胶质神经元肿瘤（RGNT）的影像学特征，以提高对本病的认识。材料与方法回顾性分析10例（男∶女=3∶7，年龄介于8～45岁）经手术病理证实的RGNT患者的影像学资料。10例行MR平扫及增强扫描，其中3例行CT平扫。结果10例肿瘤发生部位呈多样性，肿瘤位于小脑蚓部的4例；第四脑室内的2例；小脑半球、脑桥、颈髓、胸-腰髓各1例。肿瘤实性的稍多（6例），囊实性者略少（4例）。MR表现，肿瘤实性部分于T1WI肿瘤呈稍低-等信号、T2WI呈稍高信号，囊性部分于T1WI呈低信号、T2WI呈高信号。增强强化形式多样，可呈不均匀轻度-中度强化（6例），环形强化（1例），局限性结节样（2例）、斑片样（1例）。8例肿瘤周边可见轻度水肿，1例中度水肿。4例伴有幕上梗阻性脑积水，1例伴有脊髓空洞。肿瘤平扫CT表现实性部分呈稍低密度，囊性部分呈低密度，2例内可见出血。结论 RGNT好发于年轻患者，女性偏多；发生部位、影像表现具有一定特点，而术前鉴别诊断时应考虑到该病的可能。

关键词：神经元神经系统肿瘤磁共振成像病理学

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Updating conventional soil maps by mining soil–environment relationships from individual soil polygons

引用

Journal of Integrative Agriculture 2019年第2期18卷 265-278页

作者： CHENG Wei ZHU A-xing qiN Cheng-zhi qi feng State Key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101P.R.China University of Chinese Academy of Sciences Beijing 100049P.R.China Key Laboratory of Virtual Geographic Environment Ministry of EducationNanjing Normal UniversityNanjing 210023P.R.China Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application Nanjing 210023P.R.China Department of Geography University of Wisconsin-MadisonMadison 53706USA School of Environmental and Sustainability Sciences Kean UniversityUnion 07083USA

Conventional soil maps contain valuable knowledge on soil–environment *** knowledge can be extracted for use when updating conventional soil maps with improved environmental *** methods take all polygons of the same map unit on a map as a whole to extract the soil–environment *** approach ignores the difference in the environmental conditions represented by individual soil polygons of the same map *** paper proposes a method of mining soil–environment relationships from individual soil polygons to update conventional soil *** proposed method consists of three major ***,the soil–environment relationships represented by each individual polygon on a conventional soil map are extracted in the form of frequency distribution curves for the involved environmental ***,for each environmental covariate,these frequency distribution curves from individual polygons of the same soil map unit are synthesized to form the overall soil–environment relationship for that soil map unit across the mapped *** lastly,the extracted soil–environment relationships are applied to updating the conventional soil map with new,improved environmental data by adopting a soil land inference model(SoLIM)*** study applied the proposed method to updating a conventional soil map of the Raffelson watershed in La Crosse County,Wisconsin,United *** result from the proposed method was compared with that from the previous method of taking all polygons within the same soil map unit on a map as a *** results with independent soil samples showed that the proposed method exhibited better performance and produced higher accuracy.

关键词： update conventional soil map soil–environment relationships knowledge extraction individual soil polygons

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

EDTA修复肉芽肿组织中荧光定量PCR检测结核杆菌的应用

引用

临床与实验病理学杂志 2016年第6期32卷 665-668页

作者：王旭洲谢飞来许文静郑巧玲齐兴峰郑智勇南京军区福州总医院病理科福州350025

目的观察EDTA热修复炎性肉芽肿组织石蜡切片,能否提高荧光定量PCR结核/非结核分枝杆菌的检出率。方法对125例炎性肉芽肿组织石蜡切片结核/非结核分枝杆菌同时进行常规荧光定量PCR检测和EDTA热修复后荧光定量PCR检测。结果常规荧光定量PC... 详细信息

目的观察EDTA热修复炎性肉芽肿组织石蜡切片,能否提高荧光定量PCR结核/非结核分枝杆菌的检出率。方法对125例炎性肉芽肿组织石蜡切片结核/非结核分枝杆菌同时进行常规荧光定量PCR检测和EDTA热修复后荧光定量PCR检测。结果常规荧光定量PCR检测检出分枝杆菌75例(60%)阳性,其中结核杆菌74例(59.2%),非结核分枝杆菌1例(0.8%),阳性病例平均阳性基因拷贝数6.35×105/ml/例;EDTA修复后荧光定量PCR检测检出分枝杆菌88例(70.4%),其中结核杆菌83例(66.4%),非结核分枝杆菌5例(4%),阳性病例平均阳性基因拷贝数7.36×106/ml/例。两组间差异均有统计学意义(P<0.05,P<0.01)。结论 EDTA热修复后荧光定量PCR检测可以大幅度提高炎性肉芽肿组织石蜡切片的结核/非结核分枝杆菌的阳性检出率。

关键词：结核肉芽肿组织 EDTA修复荧光定量PCR检测结核/非结核分枝杆菌

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Boosting Oxygen Evolution Reaction Performance on NiFe‑Based Catalysts Through d‑Orbital Hybridization

引用

Nano-Micro Letters 2025年第1期17卷 281-292页

作者： xing Wang Wei Pi Sheng Hu Haifeng Bao Na Yao Wei Luo State Key Laboratory of New Textile Materials and Advanced Processing Technology Key Laboratory of New Textile Materials and Applications of Hubei ProvinceSchool of Materials Science and EngineeringWuhan Textile UniversityWuhan 430200People’s Republic of China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072People’s Republic of China

Anion-exchange membrane water electrolyzers(AEMWEs)for green hydrogen production have received intensive attention due to their feasibility of using earth-abundant NiFe-based *** introducing a third metal into NiFe-based catalysts to construct asymmetrical M-NiFe units,the d-orbital and electronic structures can be adjusted,which is an important strategy to achieve sufficient oxygen evolution reaction(OER)performance in ***,the ternary NiFeM(M:La,Mo)catalysts featured with distinct M-NiFe units and varying d-orbitals are reported in this *** and theoretical calculation results reveal that the doping of La leads to optimized hybridization between d orbital in NiFeM and 2p in oxygen,resulting in enhanced adsorption strength of oxygen intermediates,and reduced rate-determining step energy barrier,which is responsible for the enhanced OER *** critically,the obtained NiFeLa catalyst only requires 1.58 V to reach 1 A cm^(−2) in an anion exchange membrane electrolyzer and demonstrates excellent long-term stability of up to 600 h.

关键词： NiFe-based catalysts d-orbital coupling Oxygen evolution reaction Anion exchange membrane electrolyzer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Controllable synthesis of biomimetic wood stem nanoporous high entropy oxides catalysts for oxygen evolution reaction

引用

Nano Research 2025年第1期18卷 107-117页

作者： Wei Wang Weiqi Wang Huihui Wang xing Lu Jinwen Zhang Yunzhuo Lu School of Materials Science and Engineering Dalian Jiaotong UniversityDalian 116028China School of Intelligence and Electronic Engineering Dalian Neusoft University of InformationDalian 116023China Guangdong Key Laboratory of Materials and Equipment in Harsh Marine Environment Guangzhou Maritime UniversityGuangzhou 510725China

Bubbles generated during the oxygen evolution reaction(OER)in water splitting readily adhere to the electrode surface,thereby impeding contact between the electrolyte and the active sites,thereby increasing the ***,it is imperative to elucidate the bubble release behavior and engineer electrodes that facilitate faster bubble *** this study,inspired by the wood stem of the Norway spruce,we synthesized a gradient nanoporous high-entropy oxide(GNP-HEO)electrode with a controllable pore *** GNP-HEO,featuring a well-controlled gradient in pore size,is achieved through a straightforward strategy combining selective laser melting with selective phase *** unique gradient nanoporous structure not only facilitates bubble release but also diminishes the bubble shielding effect in OER,thereby enhancing electrocatalytic *** affect interaction of Al,Co,Cr,Fe,and Ni,coupled with the gradient nanoporous structure,yields exceptional OER performance,evidenced by an overpotential of 250 mV at a current density of 50 mA/cm^(2) and a Tafel slope of 38.0 mV/dec in 1 M *** functional theory calculations confirm that the GNPHEO adheres to the adsorbate evolution mechanism reaction pathway and exhibits significant *** work highlights a promising approach for the design and synthesis of high-performance OER electrocatalysts.

关键词： oxygen evolution reaction high-entropy oxides selective phase dis-solution selective laser melting gradient nanoporous

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

原发性肺淋巴上皮癌41例临床病理及分子遗传学分析

引用

临床与实验病理学杂志 2022年第12期38卷 1469-1474页

作者：马善宝郑巧灵延永琴齐兴峰刘庆宏曲利娟解放军联勤保障部队第九〇〇医院病理科福州350003 厦门大学附属福州市第二医院病理科福州350007 福建医科大学附属协和医院病理科福州350001

目的探讨肺淋巴上皮癌(lymphoepithelial carcinoma,LEC)的临床病理学特征及PD-1/PD-L1在肺LEC中的表达及临床意义。方法采用免疫组化法对41例肺LEC进行检测;回顾性分析41例肺LEC的临床病理学特征及随访结果,并复习相关文献。结果41例肺... 详细信息

目的探讨肺淋巴上皮癌(lymphoepithelial carcinoma,LEC)的临床病理学特征及PD-1/PD-L1在肺LEC中的表达及临床意义。方法采用免疫组化法对41例肺LEC进行检测;回顾性分析41例肺LEC的临床病理学特征及随访结果,并复习相关文献。结果41例肺LEC中,男性22例,女性19例,发病年龄27~77岁,中位年龄54岁,4例有吸烟史。临床症状主要表现为咳嗽、咳痰、咯血及胸闷,或体检发现肺部结节。免疫表型:肿瘤细胞表达CK5/6(41/41)、p40(41/41)、p63(41/41)、PD-L1(37/41),其中16例(16/41)肿瘤细胞PD-L1呈高表达;17例(17/41)肿瘤间质淋巴细胞表达PD-1。PD-L1和PD-1表达与LEC患者年龄、性别、吸烟状况、肿瘤分期等临床病理特征均无相关性(P>0.05)。11例行基因检测,均未发现RET、ROS1、EGFR、HER-2、PIK3CA、BRAF、KRAS和ALK基因突变。41例患者均行手术切除术,术后随访6~54个月,均存活,其中3例分别出现胸腔、肝和骨部位转移。结论肺LEC是一种罕见的恶性肿瘤,常见于非吸烟的年轻患者,大多数患者缺乏ALK和EGFR等常见基因突变,肿瘤细胞及间质淋巴细胞PD-L1、PD-1阳性率高,表明LEC的发生发展可能与PD-L1、PD-1表达有关,可为临床开展免疫治疗提供理论基础。

关键词：肺肿瘤淋巴上皮癌 PD-L1 PD-1 免疫治疗

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：