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Hepatocellular carcinoma and macrophage interaction induced tumor immunosuppressionvia Treg requires TLR4 signaling

World Journal of Gastroenterology 2012年第23期18卷 2938-2947页

作者： Jing Yang Jin-Xiang Zhang Hui Wang Guo-Liang Wang qing-Gang Hu qi-chang zheng Department of Hepatobiliary Surgery Union Hospital of Tongji Medical Col- lege Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Department of Emergency Surgery Union Hospital of Tongji Medical College HuazhongUniversity of Science and Technology Wuhan 430022 HubeiProvince China Department of Medical Genetics Tongji Medical College Huazhong University of Science and Technology Wu- han 430030 Hubei Province China

AIM: To investigated the interaction between toll-like receptor 4 (TLR4)-activated hepatoma cells and macrophages in the induction of tumor-immune suppression mediated by CD4+CD25high family of transcription factor P3 (FOXP3) regulatory T cells (Tregs). METHODS: The proportion of FOXP3+ Tregs was identified in peripheral blood and tumor tissues of 60 hepatocellular carcinoma (HCC) patients. TLR4 expression was examined in tumor tissues and cell lines. The correlation was examined between FOXP3+ Tregs in peripheral blood and TLR4 expression of HCC tissues. Following activation of TLR4 in H22 murine hepatoma cells pre-incubated with lipopolysaccharide (LPS) and co-cultured with macrophage cell line RAW246.7, the synthesis of cytokines tumor necrosis factor-α, CCL22, and interleukin (IL)-10 by the two cell lines was detected and analyzed. RESULTS: FOXP3+ Tregs were enriched in tumor sites, and circulating FOXP3+ Tregs were increased in HCC patients in correlation with multiple tumor foci and up-regulated TLR4 expression in HCC tissues. Semi-quantitative analysis indicated that TLR4 was over-expressed in HCC compared with the matched normal tissues. Cell cultivation experiments indicated that the mRNAs of IL-10 and CCL22 were significantly up-regulated in the RAW246.7 cell line when co-cultured with LPS preincubated H22 cells. CONCLUSION: In hepatoma cell lines, TLR4 may indirectly facilitate the recruitment of Tregs to the tumor site and promote intrahepatic metastasis through its interaction with macrophages.

关键词： CD4+CD25^high FOXP3+ regulatory T cell Toll-like receptor Tumor immunity Hepatocellular car-cinoma Macrophage

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Protection against hepatic ischemia/reperfusion injury via downregulation of toll-like receptor 2 expression by inhibition of Kupffer cell function

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World Journal of Gastroenterology 2005年第28期11卷 4423-4426页

作者： Jin-Xiang Zhang He-Shui Wu Yang Wang Hui Wang Jin-Hui Zhang qi-chang zheng Department of Emergency Surgery Union Hospital Tongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030Habei Province China Hereditary Department Tongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030Habei Province China Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM： To elucidate the mechanism of liver protection by inhibition of Kupffer cells （KCs） function.METHODS： All the animals were randomly divided into three groups. Blockade group （gadolinium chloride solution （GdCl3） injection plus ischemia/reperfusion （I/R） injury）：GdCl3 solution was injected once every 24 h for 2 d via the tail vein before I/R injury. Non-blockade group （saline solution injection plus I/R injury）： saline instead of GdCl3 as a control was injected as in the blockade group. Sham group： saline was injected without I/R injury. Liver samples were collected 4 h after blood inflow restoration. The blockade of the function of KCs was verified by immunostaining with an anti-CD68 mAb. Toll-like receptor2 （TLR2） was immunostained with a goat antimouse polydonal anti-TLR2 antibody. Membrane proteins were extracted from the liver samples and TLR2 protein was analyzed by Western blot. Portal vein serum and plasma were taken respectively at the same time point for further detection of the levels of tumor necrosis factor-α （TNF-α） and alanine aminotransferase （ALT）, an indicator of liver function.I/R injury via downregulation of theexpression of TLR2.RESULTS： Compared to non-blockade group, CD^68＋ cells significantly reduced in blockade group （OPTDI, optical density integral）： 32.97±10.55 vs 185.65±21.88, P〈0.01） and the liver function impairment was relieved partially （level of ALT： 435.89±178.37 U/L vs 890.21±272.91 U/L,P〈0.01）. The expression of TLR2 protein in blockade group significantly decreased compared to that in non- group（method of immunohistochemistry, OPDTI： 75.74±17.44 vs 170.58±25.14, P〈0.01; method of Western blot, A value： 125.89±15.49 vs 433.91±35.53, P〈0.02）. The latter correlated with the variation of CD68 staining （r = 0.745,P〈0.05）. Also the level of portal vein TNF-α decreased in blockade group compared to that in non-blockade group （84.45±14.73 ng/L vs 112.32±17.56 ng/L, P〈0.05）, butwas still higher than that in sham group （84.45±14.73

关键词： Toll-like receptor 2 Reperfusion injury Kupffer cell Liver

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Initial 12-h Operative Fluid Volume is an Independent Risk Factor for Pleural Effusion after Hepatectomy

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2016年第6期36卷 859-864页

作者： Xiang CHENG Jia-wei WU Ping SUN Zi-fang SONG qi-chang zheng Department of Hepatobiliary Surgery Union Hospital Tongji Medical College Huazhong University of Seienee and Technology Wuhan 430022 China

Pleural effusion after hepatectomy is associated with significant morbidity and prolonged hospital *** studies have addressed the risk factors for postoperative pleural ***,there are no researches concerning the role of the initial 12-h operative fluid *** aim of this study was to evaluate whether the initial 12-h operative fluid volume during liver resection is an independent risk factor for pleural effusion after *** this study,we retrospectively analyzed clinical data of 470 patients consecutively undergoing elective hepatectomy between January 2011 and December *** prospectively collected and retrospectively analyzed baseline and clinical data,including preoperative,intraoperative,and postoperative *** and multivariate analyses were carried out to identify whether the initial 12-h operative fluid volume was an independent risk factor for pleural effusion after *** multivariate analysis identified 2 independent risk factors for pleural effusion：operative time [odds ratio（OR）=10.2] and initial 12-h operative fluid volume（OR=1.0003）.Threshold effect analyses revealed that the initial 12 h operative fluid volume was positively correlated with the incidence of pleural effusion when the initial 12-h operative fluid volume exceeded 4636 m *** conclude that the initial 12-h operative fluid volume during liver resection and operative time are independent risk factors for pleural effusion after *** intravenous fluids should be restricted properly.

关键词： hepatectomy pleural effusion

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The influence of Enteral Nutrition in postoperative patients with poor liver function

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World Journal of Gastroenterology 2003年第4期9卷 843-846页

作者： qing-Gang Hu qi-chang zheng Department of Surgery,Xiehe hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China Department of Surgery Xiehe hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China. mailbox_1@***

AIM: To investigate the safety, rationality and the practicality of enteral nutritional (EN) support in the postoperative patients with damaged liver function and the protective effect of EN on the gut ***: 135 patients with liver function of Child B or C grade were randomly allocated to enteral nutrition group (EN, 65 cases), total parenteral nutrition group (TPN, 40cases) and control group (CON, 30 cases). Nutritional parameters, hepatic and kidney function indexes were measured at the day before operation, 5th and 10th day after the operation respectively. Comparison was made to evaluate the efficacy of different nutritional support. Urinary concentrations of lactulose(L) and mannitol(M) were measured by pulsed electrochemical detection(HPLC-PED)and the L/M ratio calculated to evaluate their effectiveness on protection of gut ***: No significant damages in hepatic and kidney function were observed in both EN and TPN groups between pre- and postoperatively. EN group was the earliest one reaching the positive nitrogen balance after operation and with the lowest loss of body weight and there was no change in L/M ratio after the operation (0.026±0.004) at the day 1before operation, 0.030±0.004 at the day 5 postoperative and 0.027±0.005 at the day 10 postoperative), but the change in TPN group was significant at the day 5postoperative (0.027±0.003 vs 0.038±0.009,P＜0.01).CONCLUSION: EN is a rational and effective method in patients with hepatic dysfunction after operation and has significant protection effect on the gut barrier.

关键词：肠内营养肝功能术后肾功能消化道屏障

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Serum carbohydrate antigen (CA) 19-9 as a prognostic factorin cholangiocarcinoma: A meta-analysis

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Frontiers of Medicine 2010年第4期4卷 457-462页

作者： Shang-Long LIU Zi-Fang SONG qing-Gang HU Duo SHAN Shao-Bo HU Jun LI qi-chang zheng Department of Hepatobiliary Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and PreventionBeijing 100050China

This study was performed to determine the prognostic role of preoperative serum carbohydrate antigen(CA)19-9 levels in the survival of patients with *** published up to June 1st,2010 that evaluated preoperative CA19-9 levels and the prognosis of cholangiocarcinoma were collected for *** required information for calculating indivi-dual relative risk(RR)was extracted from the studies,and a combined overall RR was *** eligible studies were *** study dealt with extra-hepatic cholangiocarcinoma,while the other eight studies analyzed intra-hepatic *** mean methodolo-gical quality score was 74.1%,ranging from 65.5%to 82.5%.The overall RR for the nine studies was 1.28(95%conﬁdence interval=1.10–1.46),and the Z-score for overall effect was 13.83(P<0.001).The association between serum CA19-9 level and lymph node involvement was also *** combined RR was 1.471(95%conﬁdence interval=0.411–5.264)and Z-score for overall effect was 0.59(P=0.553).CA19-9 levels were associated signiﬁcantly with the prognosis of patients with *** meta-analysis shows that elevation of preoperative CA19-9 levels is correlated with a poor prognosis of patients with ***,larger scale and randomized studies are needed to draw a more substantive conclusion.

关键词： cholangiocarcinoma prognostic factors serum carbohydrate antigen 19-9

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组合载荷下弹塑性圆管的不稳定界限解析

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陕西科技大学学报 1987年第4期 16-23页

作者：戚长政西北轻工业学院机械系

本文在对轴向力、内压、扭矩组合载荷作用下的弹塑性范围内的圆管,利用弹塑性理论,推导出了解本问题的虚功原理公式,采用等参二次线单元对该虚功原理公式离散化,再借助普兰特—雷斯(Prandtl—Reuss)方程导出了弹塑性有限元方程式。运用... 详细信息

本文在对轴向力、内压、扭矩组合载荷作用下的弹塑性范围内的圆管,利用弹塑性理论,推导出了解本问题的虚功原理公式,采用等参二次线单元对该虚功原理公式离散化,再借助普兰特—雷斯(Prandtl—Reuss)方程导出了弹塑性有限元方程式。运用折线负荷法和比例负荷法对圆管进行了各种变形动态解析,求出了两种负荷路径下的稳定界限应力,指出了不同负荷路径对圆管不稳定界限的影响,为设计、加工、使用弹塑性管材提供了参考依据。

关键词：组合载荷轴向力内压圆管金属管弹塑性体

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Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju zheng Yixin Li Ting Zhang Yanlin Fu Peiyan Long Xiao Gao zhengwei Wang Zhizhong Guan Xiaolan qi Wei Hong Yan Xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

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Multi boron-doping effects in hard carbon toward enhanced sodium ion storage

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Journal of Energy Chemistry 2025年第1期100卷 730-738页

作者： Peng zheng Wang Zhou Ying Mo Biao zheng Miaomiao Han qin Zhong Wenwen Yang Peng Gao Lezhi Yang Jilei Liu College of Materials Science and Engineering Hunan Joint International Laboratory of Advanced Materials and Technology for Clean Energy Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology Hunan University Greater Bay Area Institute for Innovation Hunan University changsha Research Institute of Mining & Metallurgy Co. LTD.

Hard carbon （HC） has been considered as promising anode material for sodium-ion batteries （SIBs）.The optimization of hard carbon’s microstructure and solid electrolyte interface （SEI） property are demonstrated effective in enhancing the Na+storage capability,however,a one-step regulation strategy to achieve simultaneous multi-scale structures optimization is highly ***,we have systematically investigated the effects of boron doping on hard carbon’s microstructure and interface chemistry.A variety of structure characterizations show that appropriate amount of boron doping can increase the size of closed pores via rearrangement of carbon layers with improved graphitization degree,which provides more Na+storage ***-situ Fourier transform infrared spectroscopy/electrochemical impedance spectroscopy （FTIR/EIS） and X-ray photoelectron spectroscopy （XPS） analysis demonstrate the presence of more BC3and less B–C–O structures that result in enhanced ion diffusion kinetics and the formation of inorganic rich and robust SEI,which leads to facilitated charge transfer and excellent rate *** a result,the hard carbon anode with optimized boron doping content exhibits enhanced rate and cycling *** general,this work unravels the critical role of boron doping in optimizing the pore structure,interface chemistry and diffusion kinetics of hard carbon,which enables rational design of sodium-ion battery anode with enhanced Na+storage performance.

关键词： X ray photoelectron spectroscopy

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Greedy Lattice Paths with General Weights

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Acta Mathematica Sinica,English Series 2024年第9期40卷 2213-2222页

作者： Yin Shan chang An qi zheng College of Mathematics Sichuan UniversityChengdu 610065P.R.China

Let{X_(v):v∈Z^(d)}be *** *** S(π)=Σ_(v∈π)X_(v)be the weight of a self-avoiding lattice pathπ.Let M_(n)=max{S(π):πhas length n and starts from the origin}.We are interested in the asymptotics of Mn as n→∞.This model is closely related to the first passage percolation when the weights{X_(v):v∈Z^(d)}are non-positive and it is closely related to the last passage percolation when the weights{X_(v):v∈Z^(d)}are *** general weights,this model could be viewed as an interpolation between first passage models and last passage ***,this model is also closely related to a variant of the position of right-most particles of branching random *** the two assumptions that∃α>0,E(X_(0)^(+))^(d)(log^(+)X_(0)^(+))^(d+α)0,E(X_(0)^(+))^(d)(log^(+)X_(0)^(+))^(d+α)<+∞and that E[(X_(0)^(−))^(4)]<+∞,we prove that M_(n)/n converges to the same constant M almost surely as n tends to infinity.

关键词： Last passage percolation first passage percolation greedy lattice path

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肿瘤标志物CA_(19-9)和CA_(242)联合检测在胆管癌中的诊断价值

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肝胆外科杂志 2005年第3期13卷 203-205页

作者：熊俊郑启昌胡俊华华中科技大学附属协和医院武汉430022

目的研究多肿瘤标志物蛋白质芯片联合检测CA19-9和CA242在胆管癌中的应用。方法应用多肿瘤标志物蛋白质芯片诊断系统检测75例胆管疾病患者,经术中和(或)术后病理学证实,其中胆管癌40例、胆管良性病变35例。检测两种肿瘤标志物糖类抗原19... 详细信息

目的研究多肿瘤标志物蛋白质芯片联合检测CA19-9和CA242在胆管癌中的应用。方法应用多肿瘤标志物蛋白质芯片诊断系统检测75例胆管疾病患者,经术中和(或)术后病理学证实,其中胆管癌40例、胆管良性病变35例。检测两种肿瘤标志物糖类抗原19-9(CA19-9),糖类抗原242(CA242)的改变。结果75例患者中,糖类抗原19-9和糖类抗原242同时阳性者39例,其中胆管癌患者33例,其中糖类抗原19-9大于500U/ml者28例;胆管良性病变中,有6例为糖类抗原19-9和糖类抗原242同时阳性,一例糖类抗原19-9大于500U/ml者。当糖类抗原19-9和糖类抗原242同时阳性时,诊断胆管癌敏感性为82.5%,特异性为82.9%。结论应用多肿瘤标志物蛋白质芯片诊断系统检测胆管癌,当糖类抗原19-9和糖类抗原242同时阳性时,敏感性和特异性均较好,可作为临床诊断胆管癌的较好指标。

关键词：肿瘤标志物蛋白质芯片胆管癌

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