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Increased expression of CSP and CYP genes in adult silkworm females exposed to avermectins

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Insect Science 2015年第2期22卷 203-219页

作者： Ning Xuan Xia Guo Hong-Yan Xie qi-Nian lou Xing-Bo Lu Guo-Xia Liu Jean-Francois Picimbon Biotechnology Research Center Shandong Provincial Key Laboratory for Genetic Improvement Cultivation Ecology and Physiology of Crops Shandong Academy of Agricultural Sciences Jinan Shandong Silkworm Institute Shandong Academy of Agricultural SciencesYantai plant Protection Institute Shandong Academy of Agricultural Sciences Jinan China

We analyzed 20 chemosensory protein （CSP） genes of the silkworm Bombyx mori. We found a high number of retrotransposons inserted in introns. We then analyzed expression of the 20 BrnorCSP genes across tissues using quantitative real-time polymerase chain reaction （PCR）. Relatively low expression levels of BmorCSPs were found in the gut and fat body tissues. We thus tested the effects of endectocyte insecticide abamectin （B 1 a and Blb avermectins） on BmorCSP gene expression. Quantitative real-time PCR experi- ments showed that a single brief exposure to insecticide abamectin increased dramatically CSP expression not only in the antennae but in most tissues, including gut and fat body. Furthermore, our study showed coordinate expression of CSPs and metabolic cytochrome P450 enzymes in a tissue-dependent manner in response to the insecticide. The function of CSPs remains unknown. Based on our results, we suggest a role in detecting xenobiotics that are then detoxified by cytochrome P450 anti-xenobiotic enzymes.

关键词： abamectin Bombyx mori chemosensory protein cytochrome P450 oxidase insecticide moth

Androgen signaling inhibits de novo lipogenesis to alleviate lipid deposition in zebrafish

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Dynamics of DNA in vitro evolution

动物学研究 2024年第2期 355页

作者： Jing-Yi Jia Guang-Hui Chen Ting-Ting Shu qi-Yong lou Xia Jin Jiang-Yan He Wu-Han Xiao Gang Zhai Zhan Yin

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Progress in Natural Science:Materials International 2009年第2期19卷 137-144页

作者： Xiaojing Yang 1,Xili Liu 1,Chunbo lou,qi Ouyang Center for Theoretical Biology,School of Physics,Peking University,Beijing 100871,China Center for Theoretical Biology School of Physics Peking University Beijing 100871 China

In vitro evolution has become a very important research area in recent years. From a practical point of view,it provides a powerful and reliable tool for engineering functional molecules (DNA,RNA or proteins) in reasonably short periods of time. From a theoretical point of view,since in vitro evolution is analogous to natural evolution in many respects,the study of the dynamic details of in vitro evolution may provide some instructive insights into the process of evolution. In this review,we summarize current theoretical and exper-imental studies,including several efforts made by our group,on the dynamics of DNA in vitro evolution.

关键词： DNA-TF interaction DNA in vitro evolution Natural evolution Crossover

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气红联用法研究标准桃金娘油的化学成分

光谱学与光谱分析 2007年第5期27卷 924-927页

作者：楼启正金华教育学院生化系浙江金华321000

采用毛细管气相色谱-傅里叶变换红外光谱联用技术法(气红联用,GC-FTIR),结合计算机检索对标准桃金娘油化学成分进行分析和鉴定,用气相色谱面积归一化法测定各组分的相对百分含量;经毛细管色谱从标准桃金娘油中分离出12个峰,并且采用FTI... 详细信息

采用毛细管气相色谱-傅里叶变换红外光谱联用技术法(气红联用,GC-FTIR),结合计算机检索对标准桃金娘油化学成分进行分析和鉴定,用气相色谱面积归一化法测定各组分的相对百分含量;经毛细管色谱从标准桃金娘油中分离出12个峰,并且采用FTIR法确认了所含的化合物。标准桃金娘油主要成分为三种单萜,分别为1,8-桉叶素、D-柠檬烯和α-蒎烯,含量分别为46.35%,36.83%和14.70%,三者占总量的97.88%,分析结果与GC-MS分析相一致。由于GC-FTIR分析能确定出经气相色谱分离后的有机物的异构体,故与GC-MS分析相比较,此法具有更广泛的应用价值。该研究可为综合利用开发桃金娘科植物提供科学依据。

关键词：气相色谱-傅里叶变换红外光谱法标准桃金娘油化学成分

Differences and identification on multi-time hydrocarbon generation of carboniferous-permian coaly source rocks in the Huanghua Depression,Bohai Bay Basin

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Petroleum Science 2024年第2期21卷 765-776页

作者： Jin-Jun Xu Xian-Gang Cheng Shu-Nan Peng Jun-Cai Jiang qi-Long Wu Da lou Fu-qi Cheng La-Mei Lin National Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)Qingdao 266580ShandongChina PetroChina Dagang Oilfield Company Tianjin300280China

Coal is a solid combustible mineral,and coal-bearing strata have important hydrocarbon generation potential and contribute to more than 12%of the global hydrocarbon ***,the deposition and hydrocarbon evolution process of ancient coal-bearing strata is characterized by multiple geological times,leading to obvious distinctions in their hydrocarbon generation potential,geological processes,and production,which affect the evaluation and exploration of hydrocarbon resources derived from coaly source rocks *** study aimed to identify the differences on oil-generated parent macerals and the production of oil generated from different coaly source rocks and through different oil generation *** with the analysis of previous tectonic burial history and hydrocarbon generation history,high-temperature and high-pressure thermal simulation experiments,organic geochemistry,and organic petrology were performed on the Carboniferous-Permian(C-P)coaly source rocks in the Huanghua Depression,Bohai Bay *** oil-generated parent macerals of coal's secondary oil generation process(SOGP)were mainly hydrogen-rich collotelinite,collodetrinite,sporinite,and cutinite,while the oil-generated parent macerals of tertiary oil generation process(TOGP)were the remaining small amount of hydrogen-rich collotelinite,sporinite,and cutinite,as well as dispersed soluble organic matter and unexhausted residual *** with coal,the oil-generated parent macerals of coaly shale SOGP were mostly sporinite and *** part of hydrogen-poor vitrinite,lacking hydrocarbon-rich macerals,and macerals of the TOGP,in addition to some remaining cutinite and a small amount of crude oil and bitumen from SOGP contributed to the oil *** results indicated that the changes in oil yield had a good junction between SOGP and TOGP,both coal and coaly shale had higher SOGP aborted oil yield than TOGP starting yield,and coaly shale TOGP peak oil yield was low

关键词： Thermal simulation Multi-time oil generation processes Coaly source rock Carboniferous-permian Huanghua Depression

High Power Passive Phase Locking of Four Yb-Doped Fiber Amplifiers by an All-Optical Feedback Loop

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Chinese Physics Letters 2011年第5期28卷 147-150页

作者： XUE Yu-Hao HE Bing ZHOU Jun LI Zhen FAN Yuan-Yuan qi Yun-Feng LIU Chi YUAN Zhi-Jun ZHANG Hai-Bo lou qi-Hong Shanghai Key Laboratory of All Solid-State Laser and Applied Techniques Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800 Graduate University of Chinese Academy of Sciences Beijing 100049

We report the passive phase locking of four high power Yb-doped fiber amplifiers with ring *** interference patterns at different output power are observed and the Strehl ratios are *** maximum coherent output power of the fiber array is up to 1062 W by multi-stage *** stable beam profiles of various phase relationships are observed by controlling the position of the feedback fiber,in good agreement with the calculated *** using master oscillator power-amplifier(MOPA)architecture and broadband operation of passively phased systems,higher power scaling with high beam quality appears to be feasible.

关键词： fiber passive amplifier

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有角点支座矩形板自振分析

振动与冲击 2013年第17期32卷 84-89页

作者：许琪楼郑州大学工学院郑州450002

撤去角点支座代之以角点力得板自由振动分析的基本结构。原结构振形函数表达式由基本结构所固有的基本振形和角点力所激发的附加振形组成,它应满足振动微分方程和板挠度与角点力间的微分关系。为表示板双向振动规律,基本振形在二个坐标... 详细信息

撤去角点支座代之以角点力得板自由振动分析的基本结构。原结构振形函数表达式由基本结构所固有的基本振形和角点力所激发的附加振形组成,它应满足振动微分方程和板挠度与角点力间的微分关系。为表示板双向振动规律,基本振形在二个坐标轴方向上有各自独立的振形曲线,分别符合相应方向边界所限定的、与微分方程直接关联的变形和受力特征:在支承边界上振幅为零而剪力分布不为零值;在自由边界上振幅不为零而剪力分布为零值;在自由角点处对应的振幅不为零而角点力为零值。附加振形在角点处要满足振幅与角点力的微分关系,在每条边界上要符合边界所限定的振幅与剪力分布的振动特征。并导出二邻边和对角点支承矩形板,一边支承和一角点或二角点支承矩形板的振形曲线,计算了不同边长比时板的自振频率。

关键词：弹性振动矩形板角点支承基本振形附加振形

Extraction and evolution of grader’s bionic morphological feature line using shape structure behavior function model

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International Journal of Agricultural and Biological Engineering 2024年第2期17卷 74-84页

作者： Bin qi Yubin Lan Xiaoming Sun He Zhu Yanhao Li Ke lou College of Agricultural Engineering and Food Science Shandong University of TechnologyZibo 255049ShandongChina Shandong Provincial Engineering Technology Research Center for Agricultural Aviation Intelligent Equipment Zibo 255049China

To improve the innovation of agricultural machinery product styling,this paper proposes a shape structure behavior function(SSBF)model suitable for the industrial design *** feature line evolution method combining shape grammar and genetic algorithm was used for modelling the of the grader,which not only maintains the product style characteristics but also reflects the typical identification characteristics of the bionic prototype and produces a new product modelling *** conducting cognitive and recognition experiments on product styling features,the ranking of product styling features and the contribution of each component to product styling were *** method of combining shape grammar and quadratic Bézier curve was used to express and encode feature lines,and genetic algorithm was used to evolve biomimetic forms to form product feature lines with typical biological morphological features;The extracted form bionic elements were integrated into the grader modelling design,and the interaction evaluation was carried out through the genetic algorithm evolution *** basic form elements were extracted and analyzed,and the deduction rules were formulated and *** derived feature line geometric data considered the product’s image features and the bio-inspired prototype,which can be used for the follow-up guidance of industrial design schemes.

关键词： bionic design shape-structure-behavior-function SSBF biologically inspired design BID shape grammars Quadratic Bézier curves

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四角点支承四边自由矩形板自振分析新方法

振动与冲击 2013年第3期32卷 83-86页

作者：许琪楼郑州大学工学院郑州450002

四角点支承四边自由矩形板振形函数表达式由四边自由板所固有的基本振形和角点力所激发的附加振形组成。振形函数须满足振动微分方程和板挠度与角点力间的微分关系。为表示矩形板双向振动规律,基本振形在二个坐标轴方向分别有各自的表达... 详细信息

四角点支承四边自由矩形板振形函数表达式由四边自由板所固有的基本振形和角点力所激发的附加振形组成。振形函数须满足振动微分方程和板挠度与角点力间的微分关系。为表示矩形板双向振动规律,基本振形在二个坐标轴方向分别有各自的表达式,并符合对应方向边界所限定的变形和受力特征:在对应自由边界上振幅不为零而剪力分布为零值,在自由角点处对应的四个角点力均为零值。而附加振形在角点处的振幅与角点力要符合板弯曲理论中的微分关系,在四个自由边界上对应的剪力分布均为零值。这种方法克服了现有解法中的理论缺陷,计算理念更合理。

关键词：弹性振动矩形板角点支承基本振形附加振形