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Environmental factors affecting regional differences and decadal variations in the buried flux of marine organic carbon in eastern shelf sea areas of China

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Acta Oceanologica Sinica 2021年第6期40卷 26-34页

作者： Qian Yang keming qu Shu Yang Yao Sun Yan Zhang Mingying Zhou Yellow Sea Fisheries Research Institute Chinese Fisheries Science AcademyQingdao 266071China Key Laboratory of Sustainable Development of Marine Fisheries Ministry of AgricultureQingdao 266071China Key Laboratory of Aquatic Product Processing Ministry of Agriculture and Rural AffairsChinese Academy of Fishery SciencesBeijing 100141China

To characterize environmental factors controlling decadal-scale variations in the buried flux of marine organic carbon(BFC_(m))in the eastern shelf sea areas of China(ECSS),four well preserved sediment cores collected from the central Yellow Sea mud(CYSM)area,the Yellow Sea Coastal Current(YSCC)area and the Changjiang River Estuary(CRE)were investigated in this *** the CYSM,variations in BFC_(m) were found to be dependent on variations in primary productivity and to exhibit a cyclical trend possibly related to fluctuations in the Pacific Decadal Oscillation(PDO)and the East Asian winter monsoon index(EAWM).In the YSCC,BFC_(m) likewise depends on primary *** to the 1950s,variations in BFC_(m) were similar to that of the *** the 1950s,BFC_(m) increased rapidly and exhibited maximum values in the surface layer,consistent with an increase in primary productivity caused by the input of terrestrial nutrients associated with China’s economic *** the CRE,variations in BFC_(m) were affected by several competing factors making it difficult to identify clear relationships between variations in BFC_(m) and primary *** contrast,long-term variability in BFC_(m) is more similar to changes in the Changjiang River sediment ***,it is speculated that the construction of dams along the Changjiang River may be the main cause of variations in BFC_(m) in this *** the disproportionate effects of human activities on marine environments and decadal variations in BFC_(m) in the ECSS,careful attention should be paid to regional differences in organic carbon preservation and environmental changes lest estimates of these values be made imprecise or inaccurate.

关键词： marine organic carbon buried flux primary productivity climate change human activity

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Burial fluxes and sources of organic carbon in sediments of the central Yellow Sea mud area over the past 200 years

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Acta Oceanologica Sinica 2015年第10期34卷 13-22页

作者： YANG Shu YANG Qian LIU Sai CAI Deling qu keming SUN Yao Key Laboratory for Sustainable Utilization of Marine Fisheries of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences

Long-term changes of composition, sources and burial fluxes of TOC （total organic carbon） in sediments of the central Yellow Sea mud area and their possible affecting factors are discussed in this paper. Firstly, similarity analysis is employed to confirm that the carbon burial features resulted from two collected cores are typical in the central Yellow Sea mud area where YSWC （Yellow Sea Warm Current） is prevalent. On this basis, the burial flux of TOC here was considered to be 235.5-488.4 pmol/（cm^2.a） since the first industrial revolution, accounting for about 70%-90% among burial fluxes of TC （total carbon） in the sediments. Compared TOC/TC ratio in the two cores with that in other marine sediments worldwide, we suggest that the growth of calcareous/non-calcareous organisms and dissolution of IC （inorganic carbon） are important factors controlling the TOC/TC ratio in sediment. Results of two-end mixed model based on fi13C data indicate that marine-derived organic carbon （OCa） is the main part among total burial organic carbon which accounts for a ratio over 85%. Due to the high TOC/TC ratio in the two cores, TC in the sediments also mainly exists as OCa, and the proportion of OCa is about 60%-80%. Away from the shore and relatively high primary production in upper waters are the main reasons that OCa is predominant among all burial OC in sediments of the central Yellow Sea mud area. Burial of OC in this mud area is probably mainly influenced by the human activities. Although the economic development during the late 19th century caused by the first industrial revolution in China did not obviously increase the TOC burial fluxes in the sediments, the rise of industry and agriculture after the founding of new China has clearly increased the TOC burial flux since 1950s. Otherwise, we also realize that among TC burial fluxes, TIC account for about 10%-30% in sediments of the central Yellow Sea mud area, so its burial could not be simply ignored here. Distinct from TOC

关键词： the central Yellow Sea mud area organic carbon burial flux source long-term changes human activities

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Carbon and nitrogen isotopes analysis and sources of organic matter in surface sediments from the Sanggou Bay and its adjacent areas, China

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Acta Oceanologica Sinica 2014年第12期33卷 48-57页

作者： XIA Bin CUI Yi CHEN Bijuan CUI Zhengguo qu keming MA Feifei Key Laboratory of Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fisheries Sciences

Naturally existing stable carbon and nitrogen isotopes are important in the study of sedimentary organic matter sources. To identify the sources of sedimentary organic matter in Sanggou Bay and its adjacent areas, which is characterized by high-density shellfish and seaweed aquaculture, the grain size, organic carbon （OC）, total nitrogen （TN）, carbon and nitrogen isotopic composition （δ13C andδ15N） of organic matter in the surface sediment were determined. The results showed that, in August, sedimentary OC and TN ranged from 0.17% to 0.76% and 0.04% to 0.14%, respectively. In November, OC and TN ranged from 0.23% to 0.87% and 0.05% to 0.14%, respectively. There was a significant positive correlation between OC and TN （R=0.98, P＆lt;0.0001）, indicating that OC and TN were homologous. In August, theδ13C andδ15N of organic matter varied from -23.06‰ to -21.59‰ and 5.10‰ to 6.31‰, respectively. In November,δ13C andδ15N ranged from -22.87‰ to -21.34‰ and 5.13‰ to 7.31‰, respectively. This study found that the major sources of sedimentary organic matter were marine shellfish biodeposition, seaweed farming, and soil organic matter. Using a three-end-member mixed model, we estimated that the dominant source of sedimentary organic matter was shellfish biodeposition, with an average contribution rate of 65.53% in August and 43.00% in November. Thus, shellfish farming had a significant influence on the coastal carbon cycle.

关键词： carbon nitrogen stable isotopes organic matter sources sediment Sanggou Bay

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High-throughput measurements of ciprofloxacin, clomipramine and fexofenadine hydrochlorides with an 8-channel electrical titrator

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Chinese Chemical Letters 2018年第9期29卷 1391-1394页

作者： Xuzhi Zhang Qianqian Yang Xuchang Zhang Pingping Wang Zhihui Hao Jun Zhao keming qu Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao National Laboratory for Marine Science and Technology Department of Hematology Longgang District People’s Hospital School of Chemistry and Pharmaceutical Sciences Qingdao Agriculture University

High-throughput measurements of ciprofloxacin, clomipramine and fexofenadine hydrochlorides were performed by employing an automatic 8-channel electrical titrator. Silver nitrate （AgNO3） and sodium tetraphenylborate （NaTPB） were used as titrants. When AgNO3 was used for measuring the drugs in pure form, recoveries were 97.6%-102.0% with RSD values ≤1.0%; for measuring them in pharmaceutical formulations, recoveries were 96.6%-99.1% with RSD values ≤1.0%. Batch samples of eight could be measured simultaneously and maximally 30 measurements per minute could be completed. When NaTPB was used for measuring the drugs in pure form, the recoveries were 96.8%-102.6% with RSD values 〈0.8%; for measuring them in pharmaceutical formulations, the recoveries were 97.5%-102.7% with RSD values ≤0.9%. For all analyses, no auxiliary devices or chemicals were needed and there was no requirement for changing or cleaning working electrodes between measurements. The efficiency, accuracy and precision of the proposed method make it an alternative for routine quality control analyses.

关键词： Eight-channel electrical titrator Ciprofloxacin hydrochloride Clomipramine hydrochloride Fexofenadine hydrochloride High throughput measurement Pharmaceutical analysis

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Selective feeding of bay scallop Argopecten irradians on phytoplankton community revealed by HPLC analysis of phytopigments in Bohai Sea, China

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Journal of Oceanology and Limnology 2019年第5期37卷 1746-1755页

作者： JIANG Tao WANG Longhua ZHANG Fuchong FANG Xiao LU Lin ZHANG Jihong WANG Wei qu keming CHAI Chao Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural Aff airsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266200China Qingdao Engineering Research Center for Rural Environment Qingdao Agricultural UniversityQingdao 266109China Ocean Fisheries Science Research Institute of Hebei Province Qinhuangdao 066201China

Understanding the feeding selectivity on phytoplankton by shellfi sh is currently a big challenge. In order to investigate the feeding behavior of bay scallop ( Argopecten irradians ) on phytoplankton, we compared its compositions of phytopigments in digestive glands with those in the surrounding seawater, and conducted fi ve consecutive investigations between July and November 2016 in a bay scallop culture area along coast of Qinghuangdao City, northwest of the Bohai Sea, China. Phytopigments in four-size fractionated phytoplankton of seawater (micro-(20-200 μm);nano(L)-[10-20 μm];nano(S)-[2.7-10 μm], and pico-[<2.7 μm]) and digestive glands of A . irradians were examined to investigate the selective feeding of A . irradians . Results show that fucoxanthin and peridinin constituted the major part of taxonomically diagnostic carotenoids (TDCs) in the micro- and nano(L)-phytoplankton in seawater. Compared with total phytoplankton biomass of seawater (TPB, sum of the four sizes), a substantial decrease of fucoxanthin proportion to total DCs in digestive glands was observed while that of peridinin, 19′-butanoyloxyfucoxanthin, alloxanthin and 19′-hexanoyloxy-fucoxanthin showed an obvious increase when those pigments were mainly confi ned to micro-sized phytoplankton (20-200 μm). However, zeaxanthin and prasinoxanthin were mainly confi ned to nano(s)- and pico-phytoplankton, of which the proportions in digestive glands were usually lower in TPB. The contribution of lutein to total DCs in digestive glands (with an average of 7.23%) increased compared with TPB of seawater (with an average of 0.63%) during all fi ve sampling times.

关键词： phytoplankton pigments selective feeding Argopecten irradians aquaculture

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Detection of Target Genes in Viable Bacteria and Extracellular DNA Using Loop-Mediated Isothermal Amplification Assay

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渔业科学进展 2020年第2期41卷 41-50页

作者： YANG Qianqian ZHANG Xuzhi JIANG Xiaoyu LI Yang ZHAO Jun HAO Zhihui WANG Pingping qu keming College of Marine Sciences Shanghai Ocean UniversityShanghai 201306 Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071 School of Chemistry and Pharmaceutical Sciences Qingdao Agriculture UniversityQingdao 266109

When the loop-mediated isothermal amplification(LAMP)assay is used for detecting target genes,DNA extraction is unnecessary in many *** pretreatment(***)is enough to obtain rather sensitive *** test samples without any pretreatment can be used as *** feature suggests that LAMP is superior to PCR in developing point-of-care test *** this study,using Stx1 gene from *** as model,we verified that viable cells,dead cells and extracellular DNA could function as template in the LAMP *** the incubation at 63℃,viable bacteria in the LAMP reaction mixture lysed completely within 2 min,providing DNA template for nucleic acid *** Stx1 gene in diluted culture medium,spiked tap water,spiked seawater and real seawater all could be detected,with or without the step of DNA *** found that the complex substances in real sample(*** seawater)exhibited considerable inhibitory effect on the sensitivity of the LAMP *** outcomes are meaningful for building a point-of-care strategy by employing the LAMP assay for environmental monitoring,bio-resource surveys,food safety,*** particular those based on environmental DNA.

关键词： Loop-mediated isothermal amplification DNA extraction-free Direct gene detection Viable cell Extracellular DNA

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Sea　water　environment　copper　requirement　of　egg　hatching　and　naupliar　metamorphosis　of　Peaneus　chinensis

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Acta Oceanologica Sinica 1995年第3期14卷 421-428页

作者： Yuan Youxian qu keming Liu Libo Gao Chengnian and Zhang Duxi(Yellow Sea Fisheries Research institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China)(Jimo Fisheries Bureau, Jimo 266200, China)

A new method was used to remove heavy metals in sea water. The requirement of copper in sea water ofegg hatching and naupliar metamorphosis of Penaeus chinensis was investored. A certain amount of copper ion andchelator nitrilotriacetic acid (NTA) were added in the de-heavy-metal-ionized sea water and then the cupric ion actvitywas calculated. The effects of copper ion on egg hatching and naupliar metamorphosis were observed. It was firstfound by experiments that trace ionic copper (10-10.80-10-8.80 mol/dm3) was essential to the growth and development of egg and nauplii of penaeid shrimp and chelated copper had no actions for these living processes,but in highconcentrations (>10 -7.80 mol/dm3) ionic copper was toxic. The nauplii were more sensitive to necessity and tokicityof ionic copper than eggs.

关键词： Egg hatching copper ion Penaeus chinensis,naupliar metamorphosis

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海水单胞藻培养液中微量元素的最佳浓度

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中国水产科学 1998年第2期 46-52页

作者：袁有宪曲克明辛福言中国水产科学研究院黄海水产研究所

本文通过正交试验，研究确定了铜、锌、锰、钼、钴、镍为牟氏角毛藻（Ｃｈａｅｔｏｃｅｒｏｓｍｕｌｅｒｉ）和金藻（Ｉｓｏｃｈｒｙｓｉｓｇａｌｈａｎａ）培养的必需元素，并确定了最佳浓度，依次为（１～５）×１０－８、１０－９... 详细信息

本文通过正交试验，研究确定了铜、锌、锰、钼、钴、镍为牟氏角毛藻（Ｃｈａｅｔｏｃｅｒｏｓｍｕｌｅｒｉ）和金藻（Ｉｓｏｃｈｒｙｓｉｓｇａｌｈａｎａ）培养的必需元素，并确定了最佳浓度，依次为（１～５）×１０－８、１０－９、（５～１０）×１０－８、（１～５）×１０－８、１０－７～１０－９、１０－９ｍｏｌ／Ｌ。它们应用于牟氏角毛藻和金藻的培养，可使细胞浓度和叶绿素浓度分别提高２０４％～４３２％和１９７％～２５６％。本文的研究结果较著名的Ｍ－ｆ／２配方增加了镍，并对其他的五种元素的浓度进行了修订。铜、钼、钴的浓度和Ｍ－ｆ／２配方基本相同；锰的浓度低约１个数量级；锌的浓度低约２个数量级。

关键词：微量元素单胞藻海水养殖

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海水中痕量金属元素对海洋生物作用研究的进展

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水产学报 1995年第3期19卷 250-257页

作者：袁有宪曲克明中国水产科学研究院黄海水产研究所

海水中痕量金属元素对海洋生物作用研究的进展袁有宪，曲克明（中国水产科学研究院黄海水产研究所，青岛２６６０７１）ＡＤＶＡＮＣＥＳＯＦＩＮＶＥＳＴＩＧＡＴＩＯＮＯＮＴＨＥＡＣＴＩＯＮＯＦＴＲＡＣＥＭＥＴＡＬＩＮＳＥＡＷＡ... 详细信息

海水中痕量金属元素对海洋生物作用研究的进展袁有宪，曲克明（中国水产科学研究院黄海水产研究所，青岛２６６０７１）ＡＤＶＡＮＣＥＳＯＦＩＮＶＥＳＴＩＧＡＴＩＯＮＯＮＴＨＥＡＣＴＩＯＮＯＦＴＲＡＣＥＭＥＴＡＬＩＮＳＥＡＷＡＴＥＲＴＯＭＡＲＩＮＥＯＲＧＡＮＩ...

关键词：痕量金属海洋生物海水

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渔业环境面临形势与可持续发展战略研究

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中国工程科学 2018年第5期20卷 63-68页

作者：崔正国曲克明唐启升农业部海洋渔业可持续发展重点实验室山东省渔业资源与生态环境重点实验室中国水产科学研究院黄海水产研究所山东青岛266071 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室山东青岛266071

渔业环境是水生生物赖以生存和繁衍的场所,也是渔业可持续发展的基础。总体上,我国渔业水域环境质量恶化的趋势尚未得到根本遏制,无机氮和活性磷酸盐仍是渔业水域主要的污染指标。本文在分析渔业环境现状与演变趋势的基础上,揭示了建设... 详细信息

渔业环境是水生生物赖以生存和繁衍的场所,也是渔业可持续发展的基础。总体上,我国渔业水域环境质量恶化的趋势尚未得到根本遏制,无机氮和活性磷酸盐仍是渔业水域主要的污染指标。本文在分析渔业环境现状与演变趋势的基础上,揭示了建设项目活动开发、外源污染、生态灾害和污染事故等破坏、影响渔业环境可持续发展的主要因素,识别了重点渔业水域的主要环境问题,提出了我国渔业环境绿色低碳、环境友好的可持续发展思路,确立了渔业环境发展的总体目标和到2025年、2030年两个发展阶段的具体目标,提出严控渔业水域外源污染和养殖自身污染、划定渔业生态红线、完善渔业环境监测技术体系、加强渔业水域环境修复及实施渔业创新工程等方面的建议和措施,以期为我国渔业环境的保护与管理提供参考和借鉴。

关键词：渔业环境可持续发展环境质量战略规划

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