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Protective roles of trehalose in Pleurotus pulmonarius during heat stress response

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Journal of Integrative Agriculture 2019年第2期18卷 428-437页

作者： LIU Xiu-ming WU Xiang-li GAO Wei qu ji-bin CHEN Qiang HUANG Chen-yang ZHANG jin-xia Key Laboratory of Microbial Resources Ministry of Agriculture and Rural Affaris/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China

High temperature is one of the major abiotic stresses that limit edible mushroom growth and development. The understanding of physiological alterations in response to heat stress and the corresponding mechanisms involved is vital for the breeding of heat-resistant edible mushroom strains. Although trehalose functions as a protectant against abiotic stresses in fungi, the putative role of trehalose in thermotolerance remains to be elucidated. In this study, we found heat stress inhibited the growth of two Pleurotus pulmonarius strains, heat-sensitive and less-sensitive, and the inhibition was more significant for the sensitive strain. Heat stress leads to the increase of lipid peroxidation and intracellular trehalose accumulation, with a higher level in the heat-sensitive strain, and this effect is independent of exogenous trehalose application. In addition, a lower concentration of exogenous trehalose application in sensitive strain than in less-sensitive strain was found to alleviate the inhibition of mycelium growth and further increase the intracellular trehalose concentration by heat stress. Thus, the protective effects of trehalose were more remarkable in the sensitive strain. The activities of intracellular trehalose metabolic enzymes, i.e., trehalose-6-phosphate synthase, trehalose phosphorylase and neutral trehalase, were determined, and our data indicated that the changes of these enzymes activities in the sensitive strain were more beneficial to accumulate trehalose than that in the less-sensitive strain.

关键词： edible mushroom heat stress Pleurotus pulmonarius thermotolerance trehalose

Genetic variation and population structure of the mushroom Pleurotus ferulae in China inferred from nuclear DNA analysis

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Journal of Integrative Agriculture 2016年第10期15卷 2237-2246页

作者： ZHAO Meng-ran HUANG Chen-yang WU Xiang-li CHEN Qiang qu ji-bin LI Yan-chun GAO Wei ZHANG jin-xia Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences/Key Laboratory of Microbial Resources Ministry of Agriculture Key Laboratory of Plant Diversity and Biogeography of East Asia Institute of Botany Chinese Academy of Sciences

To investigate the genetic diversity of an edible fungus Pleurotus ferulae, a total of 89 wild samples collected from six geographical locations in the Xinjiang Uygur Autonomous Region of China and two geographical locations in Italy, were analyzed using three DNA fragments including the translation elongation factor（EF1α）, the second largest subunit of t he RNA polymerase II（RPB2） and the largest subunit of the RNA polymerase II（RPB1）. The results indicated relatively abundant genetic variability in the wild resources of P. ferulae. The analysis of molecular variance（AMOVA） showed that the vast majority of the genetic variation was found within geographical populations. Both the Chinese populations and the Italian populations of P. ferulae displayed a limited genetic differentiation. The degree of differentiation between the Chinese populations and the Italian populations was obviously higher than that between the populations from the same region, and moreover the genetic differentiation among all the tested populations was correlated to the geographical distance. T he phylogeny analyses confirmed that samples from China and Italy belonged to another genetic group separated from Pleurotus eryngii. They were closely related to each other but were clustered according to their geographical origins, which implied the Chinese populations were highly differentiated from the Italian populations because of distance isolation, and the two populations from different regions might be still in the process of allopatric divergence.

关键词： genetic variation population structure genetic differentiation

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最大度为7的边染色临界图的边数

中国矿业大学学报 2010年第4期39卷 627-630页

作者：曲积彬苗连英苗正科中国矿业大学理学院江苏徐州221116 中国科学院数学与系统科学研究院北京100190 徐州师范大学数学系江苏徐州221116

根据临界图的若干引理,利用Discharging方法,通过构造新的差值转移规则,在保持图的点的总权重不变的情况下,通过差值转移使每一点的权重都大于或等于一个阀值,从而证明了与Vizing猜想有关的一个结论:对于最大度为7的临界图G,m≥270n,其... 详细信息

根据临界图的若干引理,利用Discharging方法,通过构造新的差值转移规则,在保持图的点的总权重不变的情况下,通过差值转移使每一点的权重都大于或等于一个阀值,从而证明了与Vizing猜想有关的一个结论:对于最大度为7的临界图G,m≥270n,其中m和n分别是图G的边数和顶点数.

关键词：边染色临界图度边数

边色临界图的1-因子和几乎1-因子的存在性

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中国矿业大学学报 2008年第1期37卷 139-141页

作者：苗连英苗正科段滋明曲积彬中国矿业大学理学院江苏徐州221116 徐州师范大学数学系江苏徐州221116

根据Vizing邻接引理和关于临界图的独立数的一个结论,利用图的1-因子和几乎1-因子存在的充要条件,采用结构图论的方法证明了:1)若G是2n阶Δ-临界图,且Δ≥n,δ≥n-2,则G存在1-因子;2)若G是2n+1阶Δ-临界图,且Δ≥n+1,δ≥n-2,则G存在几... 详细信息

根据Vizing邻接引理和关于临界图的独立数的一个结论,利用图的1-因子和几乎1-因子存在的充要条件,采用结构图论的方法证明了:1)若G是2n阶Δ-临界图,且Δ≥n,δ≥n-2,则G存在1-因子;2)若G是2n+1阶Δ-临界图,且Δ≥n+1,δ≥n-2,则G存在几乎1-因子.

关键词： 1-因子几乎1-因子边色数临界图独立集

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银基催化剂调控及其甲醛催化氧化性能研究

大连理工大学学报 2025年第1期65卷 17-22页

作者：黄彬曲振平大连理工大学环境学院辽宁大连116024

银基催化剂具有较好的氧化能力,在环境催化领域具有良好的应用前景.采用等体积浸渍法制备银基催化剂,考察了助剂种类、预处理气氛和温度、载体孔道结构等制备工艺条件对银基催化剂上甲醛(HCHO)催化氧化性能的影响.实验结果表明,在6%的Ag... 详细信息

银基催化剂具有较好的氧化能力,在环境催化领域具有良好的应用前景.采用等体积浸渍法制备银基催化剂,考察了助剂种类、预处理气氛和温度、载体孔道结构等制备工艺条件对银基催化剂上甲醛(HCHO)催化氧化性能的影响.实验结果表明,在6%的Ag/SBA-15催化剂中添加3%的Fe助剂能显著提高催化剂的HCHO催化氧化性能,先后采用O_(2)和H_(2)处理有助于提高催化剂活性,载体孔道结构对HCHO催化氧化活性影响较小,采用共浸渍法制备的银基催化剂的性能优于两步浸渍法制备的催化剂的性能.其中采用共浸渍法制备的AgFe/SBA-15催化剂,在O_(2)(600℃)和H_(2)(400℃)先后处理后,表现出良好的HCHO催化氧化性能,在75℃时可将HCHO完全转化为CO_(2)和H_(2)O.

关键词：甲醛催化氧化银基催化剂制备工艺条件

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食用菌产业发展历史、现状与趋势

菌物学报 2015年第4期34卷 524-540,I0001-I0006页

作者：张金霞陈强黄晨阳高巍曲积彬中国农业科学院农业资源与农业区划研究所北京100081

本文回顾了人类对食用菌认识和利用的历史,介绍了我国对食用菌栽培利用的早期贡献和近代对食用菌产业发展的贡献,以及全球食用菌产业的基本情况、欧美双孢蘑菇和亚洲食用菌产业的发展历史与现状,分析了世界食用菌产业格局的变化、生产... 详细信息

本文回顾了人类对食用菌认识和利用的历史,介绍了我国对食用菌栽培利用的早期贡献和近代对食用菌产业发展的贡献,以及全球食用菌产业的基本情况、欧美双孢蘑菇和亚洲食用菌产业的发展历史与现状,分析了世界食用菌产业格局的变化、生产方式和技术的转型,简述了食用菌科学研究的进展,同时对中国食用菌产业发展历程和现状进行了梳理,提出了全球食用菌产业将继续从发达地区向欠发达地区转移以及我国的生产方式将加快向组织化、规模化、标准化和专业化方向转变的进展。

关键词：认识利用历史产业格局技术转型发展趋势

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两个糙皮侧耳菌株的比较基因组学研究

菌物学报 2019年第12期38卷 2133-2143页

作者：王丽宁赵梦然邬向丽黄晨阳曲积彬中国农业科学院农业资源与农业区划研究所/农业农村部农业微生物资源收集与保藏重点实验室

随着糙皮侧耳基因组测序的完成,基因组数据的深入挖掘和利用成为研究重点。本研究基于较高质量的糙皮侧耳CCMSSC00389-1和CCMSSC03989-1基因组图谱,开展了种内比较基因组学分析。CCMSSC00389-1和CCMSSC03989-1的11条染色体序列表现出良... 详细信息

随着糙皮侧耳基因组测序的完成,基因组数据的深入挖掘和利用成为研究重点。本研究基于较高质量的糙皮侧耳CCMSSC00389-1和CCMSSC03989-1基因组图谱,开展了种内比较基因组学分析。CCMSSC00389-1和CCMSSC03989-1的11条染色体序列表现出良好的共线性,且分别含有89.92%和91.68%的保守基因。对菌株特有基因的GO富集分析表明,两菌株各自分化出一些独特的调控方式或途径。CCMSSC00389-1和CCMSSC03989-1之间共存在931542个单核苷酸多态性位点,231654个插入/缺失和9221个结构变异。对与遗传变异重叠基因的GO富集分析表明,碳水化合物降解、物质运输/催化和调控/蛋白活性相关的基因分别容易发生SNP、In/Del和SV变异。CCMSSC00389-1中50.326kb缺失序列的断点两侧含有双链断裂修复(DSB)信号,推测DSB介导50.326kb序列的缺失。

关键词：比较分析遗传变异 GO富集分析生物信息学

Boosting bidirectional sulfur conversion enabled by introducing boron-doped atoms and phosphorus vacancies in Ni2P for lithium-sulfur batteries

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Journal of Energy Chemistry 2025年第1期100卷 760-769页

作者： Lin Peng Yu Bai Hang Li Meixiu qu Zhenhua Wang Kening Sun Advanced Research Institute of Multidisciplinary Science Beijing Institute of Technology Beijing Key Laboratory of Chemical Power Source and Green Catalysis School of Chemistry and Chemical Engineering Beijing Institute of Technology

Lithium-sulfur （Li-S） batteries have gained great attention due to the high theoretical energy density and low cost,yet their further commercialization has been obstructed by the notorious shuttle effect and sluggish redox ***,we supply a strategy to optimize the electron structure of Ni2P by concurrently introducing B-doped atoms and P vacancies in Ni2P （Vp-B-Ni2P）,thereby enhancing the bidirectional sulfur *** study indicates that the simultaneous introduction of B-doped atoms and P vacancies in Ni2P causes the redistribution of electron around Ni atoms,bringing about the upward shift of d-band center of Ni atoms and effective d-p orbital hybridization between Ni atoms and sulfur species,thus strengthening the chemical anchoring for lithium polysulfides （LiPSs） as well as expediting the bidirectional conversion kinetics of sulfur ***,theoretical calculations reveal that the incorporation of B-doped atoms and P vacancies in Ni2P selectively promotes Li2S dissolution and nucleation ***,the Li-S batteries with Vp-B-Ni2P-separators present outstanding rate ability of 777 m A h g-1at 5 C and high areal capacity of 8.03 mA h cm-2under E/S of 5μL mg-1and sulfur loading of 7.20 mg *** work elucidates that introducing heteroatom and vacancy in metal phosphide collaboratively regulates the electron structure to accelerate bidirectional sulfur conversion.

关键词： Lithium sulfur batteries

Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si Wang Weiqi Zhang jing qu Guang-Hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis