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转录因子NKX2.1促进诱导多能干细胞向肺干细胞的分化

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中国组织工程研究 2025年第36期29卷 7790-7796页

作者：邓丽刘洋王慧杨秋董明清成都市第五人民医院医学研究与转化中心四川省成都市611130

目的:探究NKX2.1对诱导多能干细胞向肺干细胞方向分化的影响。方法:体外培养人诱导多能干细胞,采用实时荧光定量PCR和免疫荧光检测多能干细胞特异基因的表达;通过瞬时转染向人诱导多能干细胞中过表达NKX2.1,再诱导其向肺干细胞方向分化... 详细信息

目的:探究NKX2.1对诱导多能干细胞向肺干细胞方向分化的影响。方法:体外培养人诱导多能干细胞,采用实时荧光定量PCR和免疫荧光检测多能干细胞特异基因的表达;通过瞬时转染向人诱导多能干细胞中过表达NKX2.1,再诱导其向肺干细胞方向分化,诱导分化7 d采用实时荧光定量PCR、免疫荧光检测FoxA2、SOX9和P63的表达,诱导分化13 d采用免疫荧光检测肺泡细胞标记分子SPB和SPC的表达。结果与结论:(1)诱导多能干细胞紧密集结呈典型克隆样生长,显著高表达干细胞特异表达基因OCT-4、SOX2和NANOG;(2)与未转染对照组相比,过表达NKX2.1组人诱导多能干细胞中NKX2.1表达显著升高(P<0.000 1);(3)诱导分化7 d,与未转染对照组相比,过表达NKX2.1组肺干细胞相关标记物FoxA2、SOX9和P63的表达显著升高(P<0.000 1);(4)诱导分化13 d,与未转染对照组相比,过表达NKX2.1组肺泡细胞标记分子SPB和SPC荧光强度显著增加。结果表明NKX2.1可以促进诱导多能干细胞向肺干细胞方向分化。

关键词：诱导多能干细胞肺干细胞 NKX2.1 定型内胚层前肠内胚层肺损伤工程化干细胞

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Wdr63 Deletion Aggravates Ulcerative Colitis Likely by Affecting Th17/Treg Balance and Gut Microbiota

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生物化学与生物物理进展 2025年第1期52卷 209-222页

作者： ZHU Hao ZHU Meng-Yuan CAO yang-yang yang qiu-Bo FAN Zhi-Peng Beijing Institute for Dental Research Capital Medical University School of StomatologyBeijing 100050China Beijing Laboratory of Oral Health Capital Medical UniversityBeijing 100069China Research Unit of Tooth Development and Regeneration Chinese Academy of Medical SciencesBeijing 100069China

Objective Ulcerative colitis is a prevalent immunoinflammatory ***17/Treg cell imbalance and gut microbiota dysregulation are key factors in ulcerative colitis *** actin cytoskeleton contributes to regulating the proliferation,differentiation,and migration of Th17 and Treg ***63,a gene containing the WD repeat domain,participates in the structure and functional modulation of actin *** research indicates that WDR63 may serve as a regulator of cell migration and metastasis via actin polymerization *** article aims to explore the effect of Wdr63 deletion on Th17/Treg cells and ulcerative *** We constructed Wdr63-/-mice,induced colitis in mice using dextran sulfate sodium salt,collected colon tissue for histopathological staining,collected mesenteric lymph nodes for flow cytometry analysis,and collected healthy mouse feces for microbial diversity *** Compared with wild-type colitis mice,Wdr63-/-colitis mice had a more pronounced shortening of colonic tissue,higher scores on disease activity index and histological damage index,Treg cells decreased and Th17 cells increased in colonic tissue and mesenteric lymph nodes,a lower level of anti-inflammatory cytokine IL-10,and a higher level of pro-inflammatory cytokine *** addition,WDR63 has shown positive effects on maintaining intestinal microbiota *** maintains the balance of Bacteroidota and Firmicutes,promoting the formation of beneficial intestinal bacteria linked to immune *** Wdr63 deletion aggravates ulcerative colitis in mice,WDR63 inhibits colonic inflammation likely by regulating Th17/Treg balance and maintains intestinal microbiota homeostasis.

关键词： Wdr63 Th17/Treg ulcerative colitis inflammation immune microbiology

Method for Evaluating Digital Video Electromagnetic Information Leakage from Video Cable

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Chinese Journal of Electronics 2021年第5期30卷 978-985页

作者： WANG Sen qiu yang TIAN Jin AECC Aero Engine Control System Institute Electromagnetic Compatibility Laboratory Xidian University

Electromagnetic emissions from electrical information equipment may contain useful information and lead to information leakage. In order to detect the electromagnetic information leakage of digital video cable,a rapid testing method for computer video information leakage detection without reconstructing the displayed image is proposed. In this method, the electromagnetic radiation spectrum of typical image transmitted by HDMI cable is simulated. Then the electromagnetic radiation spectrum of the computer when transmitting the same image as the typical image is obtained by conduction and radiation EMC test methods. Finally, the correlation between the spectrum of simulation and the test spectrum is analyzed, and the correlation coefficient is used to judge whether the computer has produced the electromagnetic leakage of video information. The analysis and test results show that when the correlation coefficient is greater than 0.5, the electromagnetic leakage of computer video information can be judged.

关键词： lead rapid testing method computer video information leakage detection displayed image electrical information equipment evaluating digital video electromagnetic information leakage test spectrum typical image electromagnetic leakage electromagnetic radiation spectrum HDMI cable correlation coefficient electromagnetic emissions digital video broadcasting electromagnetic compatibility digital video cable

Hydrogen Bonding Effects on the Photophysical Properties of 2,3-dihydro-3-keto- 1H-pyrido[3,2,1-kl] phenothiazine

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Chinese Journal of Chemical Physics 2012年第3期25卷 254-260,373页

作者： Song-qiu yang Guo-zhong He State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical Physics ChineseAcademy of Sciences Dalian 115023 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Time-dependent density functional theory （TDDFT） and femtosecond transient absorption spectroscopy were used to investigate the photophysical properties of 2,3-dihydro-3-keto-lH- pyrido[3,2,1-kl]phenothiazine （PTZ4） and 3-keto-lH-pyrido[3,2,1-kl]phenothiazine （PTZ5）. The calculated results obtained from TDDFT suggest that the red-shifts of the absorption spectra of these two fluorophores in methanol are due to the formation of hydrogen-bonded complexes at the ground state. Four conformers of PTZ4 were obtained by TDDFT. The two fluorescence peaks of PTZ4 in tetrahydrofuran （THF） came from the ICT states of the four conformers. The fluorescence of PTZ4 in THF showed a dependence on the excitation wavelength because of butterfly bending. The excited state dynamics of PTZ4 in THF and methanol were obtained by transient absorption spectroscopy. The lifetime of the excited PTZ4 in methanol was 53.8 ps, and its relaxation from the LE state to the ICT state was completed within several picoseconds. The short lifetime of excited PTZ4 in methanol was due to the formation of out-of-plane model hydrogen bonds between PTZ4 and methanol at the excited state.

关键词： 2,3-dihydro-3-keto-lH-pyrido[3,2,1-kl]phenothiazine Time-dependent densityfunctional theory Femtosecond transient absorption spectroscopy Hydrogen bonding effect

Explicit G2-constrained Merging of a Pair of Bezier Curves by Control Point Optimization

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自动化学报 2014年第7期40卷 1505-1508页

作者： LU Li-Zheng qiu Yu-yang School of Statistics and Mathematics Zhejiang Gongshang University Hangzhou 310018 China

This paper presents a simple and explicit method for G~2-constrained merging of a pair of Bezier curves by minimizing the l_2 distance defined in terms of control points. After expressing the l_2 distance as a quadratic function of two parameters, the optimally merged curve can be explicitly obtained, which is achieved by control point optimization such that the l_2 distance is minimized. The existence of the unique solution is shown by proving that the l_2 distance is convex. The proposed method is explicit and efficient since it is non-iterative and expressed by known control points. Numerical examples demonstrate the effectiveness of the new method.

关键词：贝塞尔曲线控制点合并 G2 优化二次函数距离最小化

Tightening the Penrose inequality

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Science China(Physics,Mechanics & Astronomy) 2022年第12期65卷 122-130页

作者： Hossein Khodabakhshi Hong Lü Run-qiu yang Center for Joint Quantum Studies Department of PhysicsSchool of ScienceTianjin UniversityTianjin300350China

The Penrose inequality estimates the lower bound of the mass of a black hole in terms of the area of its *** bound is relatively loose for extremal or near extremal black *** propose a new Penrose-like inequality for static black holes involving the mass,area of the black hole event horizon and *** inequality includes the Penrose inequality as its corollary,and it is saturated by both the Schwarzschild and Reissner-Nordstr?m black *** the spherically symmetric case,we prove this new inequality by assuming both the null and trace energy conditions.

关键词： black hole Penrose-like inequality trace energy condition

氮磷添加对宁夏荒漠草原植物物种多样性和群落稳定性的影响

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草地学报 2025年第1期33卷 222-230页

作者：杨云涛邱开阳王玉娟司浩宇鲍平安黄业芸何毅谢应忠宁夏大学林业与草业学院宁夏银川750021 平罗县农业综合执法大队宁夏石嘴山753000 宁夏草牧业工程技术研究中心宁夏银川750021 宁夏大学农业农村部饲草高效生产模式创新重点实验室宁夏银川750021 西北土地退化与生态恢复国家重点实验室培育基地宁夏银川750021

为深入了解荒漠草原植物物种多样性和群落稳定性对N、P添加的响应,本研究以宁夏盐池县荒漠草原为研究对象,自2020年开始,每年开展N、P添加试验,连续进行3年。试验采用随机区组设计,N肥选用尿素,P肥选用过磷酸钙,本文使用2022年9月的植... 详细信息

为深入了解荒漠草原植物物种多样性和群落稳定性对N、P添加的响应,本研究以宁夏盐池县荒漠草原为研究对象,自2020年开始,每年开展N、P添加试验,连续进行3年。试验采用随机区组设计,N肥选用尿素,P肥选用过磷酸钙,本文使用2022年9月的植被调查数据。结果表明:(1)高水平N复合中、高水平P添加显著降低了物种丰富度指数和Shannon-Wiener指数,高水平N复合中水平P添加显著降低了Simpson指数;(2)Godron稳定性分析发现,对照组欧式距离最大,群落稳定性最低;在单独添加P时,群落稳定性随着P添加量的增加而逐渐增强;(3)荒漠草原物种多样性与群落稳定性表现为正相关关系。综上所述,高水平的N、P添加可以通过抑制荒漠草原植物生长,使得物种多样性降低,单独P添加以及中、低水平的P与高水平的N复合添加可有效提高群落的稳定性。

关键词：养分添加退化荒漠草原生态修复欧式距离植物多样性

Some Sufficient Conditions for Tunnel Numbers of Connected Sum of Two Knots Not to Go Down

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Acta Mathematica Sinica,English Series 2011年第11期27卷 2229-2244页

作者： Guo qiu yang Feng Chun LEI School of Astronautics Harbin Institute of Technology Harbin 150001 P. R. China Department of Mathematics Harbin Institute of Technology Harbin 150001 P. R. China School of Mathematical Sciences Dalian University of Technology Dalian 116024 P. R. China

In this paper, we show the following result： Let Ki be a knot in a closed orientable 3- manifold Mi such that （Mi,Ki） is not homeomorphic to （S^2 × S^1,x0 × S^1）, i = 1,2. Suppose that the Euler Characteristic of any meridional essential surface in each knot complement E（Ki） is less than the difference of one and twice of the tunnel number of Ki. Then the tunnel number of their connected sum will not go down. If in addition that the distance of any minimal Heegaard splitting of each knot complement is strictly more than 2, then the tunnel number of their connected sum is super additive. We further show that if the distance of a Heegaard splitting of each knot complement is strictly bigger than twice the tunnel number of the knot （twice the sum of the tunnel number of the knot and one, respectively）, then the tunnel number of connected sum of two such knots is additive （super additive, respectively）.

关键词： Tunnel number Heegaard splitting Heegaard distance meridional surface

碳、氮、钾添加对荒漠草原凋落物分解特征的影响

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草业学报 2025年第2期34卷 41-53页

作者：骆欣怡邱开阳金涛鲍平安黄业芸何毅谢应忠宁夏大学林业与草业学院宁夏银川750021 宁夏草牧业工程技术研究中心宁夏银川750021 农业农村部饲草高效生产模式创新重点实验室宁夏银川750021 盐池北部荒漠草原宁夏野外科学观测研究站宁夏银川750021 宁夏贺兰山国家级自然保护区管理局宁夏银川750021

凋落物分解是草地生态系统能量流动和物质循环的重要过程,而荒漠草原凋落物分解如何响应碳(C)、氮(N)、钾(K)添加尚不清楚,揭示这一关系对深入理解荒漠草原养分循环过程具有重要意义。本研究选用网袋分解法,进行随机区组设计,在试验样... 详细信息

凋落物分解是草地生态系统能量流动和物质循环的重要过程,而荒漠草原凋落物分解如何响应碳(C)、氮(N)、钾(K)添加尚不清楚,揭示这一关系对深入理解荒漠草原养分循环过程具有重要意义。本研究选用网袋分解法,进行随机区组设计,在试验样地分别设置C_(1)(0 g·m^(-2)·a^(-1))、C_(2)(52.54 g·m^(-2)·a^(-1))、C_(3)(705.09 g·m^(-2)·a^(-1));N_(1)(0 g·m^(-2)·a^(-1))、N_(2)(5 g·m^(-2)·a^(-1))、N_(3)(20 g·m^(-2)·a^(-1));K_(1)(0 g·m^(-2)·a^(-1))、K_(2)(9.0 g·m^(-2)·a^(-1))、K_(3)(18.0 g·m^(-2)·a^(-1))3个C、N、K浓度梯度,研究外源C、N、K添加对宁夏荒漠草原优势种短花针茅和赖草凋落物分解特征的影响。结果表明:(1)C、N、K添加对荒漠草原两个物种凋落物分解的影响具有显著差异(P短花针茅;(3)短花针茅和赖草凋落物均在秋季分解较快。综上,同一生境不同物种凋落物分解速率存在差异,高添加量的K、N和低添加量的C相较于其他养分添加梯度,对凋落物分解速率的促进作用更明显。

关键词：荒漠草原凋落物分解特征养分添加