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Energy Buildup,Flux Confinement and Helicity Accumulation in the Solar Corona

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Chinese Journal of Astronomy and Astrophysics 2006年第1期6卷 77-86页

作者： you-qiu Hu wen Li School of Earth and Space Sciences University of Science and Technology of China Hefei230026

Starting from a dipole field ment of field lines on the photosphere, and a given distribution of footpoint displacewe find axisymmetric, force-free field solutions in spherical coordinates that have the same distribution of normal field on the photosphere and magnetic topology as the dipole field. A photospheric shear is introduced in the azimuthal direction in a region that strides across the equator and ends at latitude λs. The footpoint displacement has a sine distribution in latitude and a peak amplitude of φm. The magnetic energy E, azimuthal flux Fφ, and magnetic helicity lit in the solar corona are then calculated for each force-free field solution. It is found that for a given shear region range λs, all of the three quantities increase monotonically with increasing φm. In particular, both Fφ and HT have a linear dependence on φm. When φm reaches a certain critical value φmc, the force-free field loses equilibrium, leading to a partial opening of the field and the appearance of a current sheet in the equatorial plane. At this point, E, Fφ and HT reach their maximum values, Ec, Fφc and HTo Ec increases, and Fφc and HTc decrease with decreasing λs. It is found that Ec is always smaller than the open field energy, in agreement with the Aly conjecture. Of the three critical parameters, Ec has the weakest dependence on λs. Therefore, if one is interested in the transition of a magnetic configuration from a stable state to a dynamic one, the magnetic energy is probably the most appropriate marker of the transition.

关键词： Sun: magnetic fields -- Sun: force-free fields methods: numerical

GsMAPK4,a positive regulator of soybean tolerance to salinity stress

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Journal of Integrative Agriculture 2019年第2期18卷 372-380页

作者： qiu you-wen FENG Zhe FU Ming-ming YUAN Xiao-han LUO Chao-chao YU Yan-bo FENG Yan-zhong WEI Qi LI Feng-lan College of Life Science Northeast Agricultural UniversityHarbin 150030P.R.China Heilongjiang Institute of Education Harbin 150030P.R.China Heilongjiang Academy of Agricultural Sciences Harbin 150086P.R.China

Salt stress is one of the major factors affecting plant growth and yield in soybean under saline soil condition. Despite many studies on salinity tolerance of soybean during the past few decades, the detailed signaling pathways and the signaling molecules for salinity tolerance regulation have not been clarified. In this study, a proteomic technology based on two-dimensional gel electrophoresis(2-DE) and mass spectrometry(MS) were used to identify proteins responsible for salinity tolerance in soybean plant. Real-time quantitative PCR(qRT-PCR) and Western blotting(WB) were used to verify the results of 2-DE/MS. Based on the results of 2-DE and MS, we selected glucosyltransferase(GsGT4), 4-coumarate, coenzyme A ligase(Gs4 CL1), mitogen-activated protein kinase 4(GsMAPK4), dehydration responsive element binding protein(GsDREB1), and soybean cold-regulated gene(GsSRC1) in the salinity tolerant soybean variety, and GsMAPK4 for subsequent research. We transformed soybean plants with mitogen-activated-protein kinase 4(GsMAPK4) and screened the resulting transgenics soybean plants using PCR and WB, which confirmed the expression of GsMAPK4 in transgenic soybean. GsMAPK4-overexpressed transgenic plants showed significantly increased tolerance to salt stress, suggesting that GsMAPK4 played a pivotal role in salinity tolerance. Our research will provide new insights for better understanding the salinity tolerance regulation at molecular level.

关键词： soybean salinity tolerance two-dimensional gel electrophoresis GsMAPK4

Screen of Bovine Mammary Gland Epithelial Cell Specifcity Promotor

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Journal of Northeast Agricultural University(English Edition) 2012年第3期19卷 72-75页

作者： Liu Xiao-fei Li Qing-zhang qiu you-wen Gao Xue-jun Key Laboratory of Dairy Science of Education Ministry Northeast Agricultural University Harbin 150030 China Key Laboratory for Food Science and Engineering Harbin University of Commerce Harbin 150076 China

Three lactoproteins （α-Sl-casein, β-lactoglobulin, and β-casein） promotors were cloned, sequenced and compared relative luciferase expression. The results showed that the promotor activity of bovine α-S1-casein gene was the best, and would be used to produce pharmaceutically and medically important proteins in the mammary gland of transgenic animals and also for the construction of an inducible eukaryotic expression vector.

关键词： lactoprotein screen specificity promotor

A highly selective electrochemical impedimetric sensor for imidacloprid determination based on WO_(3)/MoS_(2)nanosheets/molecularly imprinted polymer composite

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Rare Metals 2024年第3期43卷 1309-1315页

作者： Long-Hua Ding Ya-wen Wang qiu-wen Li Li-Li Zhang Ai-Zhu Wang Institute for Advanced Interdisciplinary Research School of Chemistry and Chemical EngineeringUniversity of JinanJinan250022China HCA Florida Healthcare Westside/Northwest Hospital Internal Medicine PlantationFL33324USA

In this study,an electrochemical impedimetric sensor was established for imidacloprid(IMI)detection based on WO_(3)/MoS_(2)and molecular imprinted polymer(MIP)*** layer was prepared on electrode surface by electropolymerization of o-phenylenediamine(o-PD)in the presence of IMI template,which ensured the specific rec-ognization toward IMI.

关键词： electrochemical polymer polymerization

Implicit Function Based Open-loop Analysis Method for Detecting the SSR Using Identified System Parameters

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CSEE Journal of Power and Energy Systems 2024年第5期10卷 2016-2026页

作者： Luonan qiu Tianhao wen Yang Liu Q.H.Wu School of Electric Power Engineering South China University of TechnologyGuangzhou 510640China

This paper proposes an implicit function based open-loop analysis method to detect the subsynchronous resonance(SSR),including asymmetric subsynchronous modal attraction(ASSMA)and asymmetric subsynchronous modal repulsion(ASSMR),of doubly-fed induction generator based wind farms(DFIG-WFs)penetrated power *** some important parameters of DFIG-WF are difficult to obtain,reinforcement learning and least squares method are applied to identify those important *** predicting the location of closed-loop subsynchronous oscillation(SSO)modes based on the calculation of partial differentials of characteristic equation,both ASSMA and ASSMR can be *** proposed method in this paper can select SSO modes which move to the right half complex planes as control parameters ***,the proposed open-loop analysis method is adaptive to parameter *** studies are carried out on the 4-machine 11-bus power system to verify properties of the proposed method.

关键词： Open-loop modal analysis reinforcement learning based parameter identification subsynchronous resonance

Dynamic steric confinement effect expands the frontier of Cu-catalyzed asymmetric propargylic aminations

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Science China Chemistry 2024年第10期67卷 3181-3183页

作者： Peng Chen Mao-Mao Zhang wen-Jing Xiao Liang-qiu Lu Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction Ministry of EducationCollege of ChemistryCentral China Normal UniversityWuhan 430079China Wuhan Institute of Photochemistry and Technology Wuhan 430082China State Key Laboratory for Oxo Synthesis and Selective Oxidation Lanzhou Institute of Chemical Physics(LICP)Chinese Academy of SciencesLanzhou 730000China School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007China

Chiralα-tertiary amines and related azacycles are commonly found in many natural products,pharmaceuticals and agrochemicals[1].Copper-catalyzed asymmetric propargylic amination(APA)reactions have been established as a reliable method to access enantio-enriched propargylic amines,largely due to the seminal contribution of Nishibayashi et al.[2]and van Maarseveen et al.[3]in 2008.

关键词： catalyzed asymmetric amines

Establishment of TaqMan Real-time Quantitative PCR Assay for Foreign Gene Copy Numbers in Transgenic Soybean

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Journal of Northeast Agricultural University(English Edition) 2012年第4期19卷 48-52页

作者： qiu you-wen Gao Xue-jun Qi Bang-ruo Li Lu Zhen Zhen Life Science and Biotechnique Research Center Northeast Agricultural University/Testing and Inspecting Center for GMO

TaqMan quantitative PCR technique was used to detect the copies of exogenous CaMV35S flanks sequence in transgenic soybean. With soybean lectin as the endogenous reference gene, and gene complex DNA in non-GMO soybeans as the endogenous reference standard, the gradient dilution method was used to separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies, and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation. The standard curve equation of endogenous reference gene was y =–3.422x＋35.201, R2=0.998; the standard curve equation of exogenous gene was y =–3.495x＋35.303, R2=0.999. The sample copies was got by putting Ct value into the standard curve equation, and it was the ratio of exogenous gene and reference gene. We found that CaMV35S gene in transgenic soy was single copy.

关键词： real-time PCR transgenic soybean copy lectin CaMV35S flanking sequence

The role of the auxin-response genes MdGH3.1 and MdSAUR36 in bitter pit formation in apple

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Horticultural Plant Journal 2024年第5期10卷 1085-1098页

作者： Daqing Huang wen Peng Na Gong Lina qiu Yongzhang Wang Haiyong Qu College of Horticulture Qingdao Agricultural UniversityQingdaoShandong 266109China

Apples often exhibit bitter pits in response to metabolic disorders during ripening and storage;however, the mechanisms underlying the bitter pit(BP) development remain unclear. Here, metabolome and transcriptome analyses were performed to investigate BP pulp of 'Fuji'. Two auxin-response genes, MdGH3.1 and MdSAUR36, were screened. Their expression as well as the auxin content in BP pulp were found to be higher than those in healthy pulp(P < 0.01). In the field, excess CO(NH2)2increased the incidence of BP. Moreover, the auxin content and MdGH3.1 expression increased in apples after nitrogen fertilization. On Day 30 before harvest, the two genes were transiently transferred to the fruit, and 20.69% and 23.21% of BP fruits were harvested. After 10 μmol·L-1auxin was infiltrated at low pressure into postharvest fruit, the increase in MdGH3.1 expression occurred earlier than that in MdSAUR36. MdGH3.1 increased the expression of MdSAUR36, but MdSAUR36 did not increase expression of MdGH3.1. Therefore, we suggest that MdGH3.1 acts upstream of MdSAUR36 during BP formation and that these genes induce BP formation by regulating auxin and phenylpropanoid biosynthesis.

关键词： Apple Malusdomestica Auxin Bitter pit Flavonoids Nitrogen fertilizer Widely targeted metabolomics

BMSC-derived Exosomes Ameliorate Peritoneal Dialysis-associated Peritoneal Fibrosis via the Mir-27a-3p/TP53 Pathway

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Current Medical Science 2024年第2期44卷 333-345页

作者： Jun-li ZHAO Lin ZHAO qiu-nan ZHAN Miao LIU Ting ZHANG wen-wen CHU Department of Nephrology Shanghai University of Medicine&Health Sciences Affiliated Zhoupu HospitalShanghai 201318China Orthopedic Department Guangming Traditional Chinese Medicine Hospital of Pudong New AreaShanghai 201399China

Objective:Peritoneal fibrosis(PF)is the main cause of declining efficiency and ultrafiltration failure of the peritoneum,which restricts the long-term application of peritoneal dialysis(PD).This study aimed to investigate the therapeutic effects and mechanisms of bone marrow mesenchymal stem cells-derived exosomes(BMSC-Exos)on PF in response to ***:Small RNA sequencing analysis of BMSC-Exos was performed by second-generation sequencing.C57BL/6J mice were infused with 4.25%glucose-based peritoneal dialysis fluid(PDF)for 6 consecutive weeks to establish a PF model.A total of 36 mice were randomly divided into 6 groups:control group,1.5%PDF group,2.5%PDF group,4.25%PDF group,BMSC-Exos treatment group,and BMSC-Exos+TP53 treatment *** transcription quantitative polymerase chain reaction(RT-qPCR)was performed to measure the expression level of miR-27a-3p in BMSC-Exos and peritoneum of mice treated with different concentrations of *** and Masson staining were performed to evaluate the extent of *** therapeutic potential of BMSC-Exos for PF was examined through pathological examination,RT-qPCR,Western blotting,and peritoneal function ***-mesenchymal transition(EMT)of HMrSV5 was induced with 4.25%*** were divided into control group,4.25%PDF group,BMSC-Exos treatment group,and BMSC-Exos+TP53 treatment *** Counting Kit-8 assay was used to measure cell viability,and transwell migration assay was used to verify the capacity of BMSC-Exos to inhibit EMT in HMrSV5 ***:Small RNA sequencing analysis showed that miR-27a-3p was highly expressed in BMSC-derived exosomes compared to *** RT-qPCR results showed that the expression of miR-27a-3p was upregulated in BMSC-Exos,but decreased in PD *** found that PF was glucose concentration-dependently enhanced in the peritoneum of the PD *** with the control mice,the PD mice showed high solute transport and decreased ultrafiltration volume as well as an obvious f

关键词： peritoneal fibrosis bone marrow mesenchymal stem cell-derived exosomes miR-27a-3p TP53 epithelialmesenchymal transition