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2016—2023年上海市浦东新区和松江区畜禽源大肠埃希菌耐药性比较

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中国兽医杂志 2025年第1期61卷 38-47页

作者：方雅红张妤孙冰清姜芹龚志亮李闽商军张亦菲上海市动物疫病预防控制中心上海长宁201103 上海市浦东新区畜牧水产技术推广中心上海浦东201299 上海市松江区动物疫病预防控制中心上海松江201699

为了调查2016—2023年上海市浦东新区和松江区(下文简称“两区”)畜禽源大肠埃希菌(***)的耐药性变迁情况,本试验从两区畜禽养殖场分别采集401和490份畜禽肛拭子进行大肠埃希菌的分离鉴定,并调查养殖场的抗菌药物使用情况;采用微量肉汤... 详细信息

为了调查2016—2023年上海市浦东新区和松江区(下文简称“两区”)畜禽源大肠埃希菌(***)的耐药性变迁情况,本试验从两区畜禽养殖场分别采集401和490份畜禽肛拭子进行大肠埃希菌的分离鉴定,并调查养殖场的抗菌药物使用情况;采用微量肉汤稀释法测定分离的大肠埃希菌对10大类14种抗菌药物的敏感性。结果显示,两区不同养殖场的抗菌药物使用情况存在差异。两区分别分离到333和350株大肠埃希菌,分离率为83.04%和71.43%。两区分离菌株对四环素(87.09%,82.00%)、磺胺异噁唑(77.78%,82.00%)、氨苄西林(82.58%,70.30%)和复方新诺明(70.87%,76.90%)的耐药率较高,对头孢他啶(6.61%,6.90%)和黏菌素(0.90%,0.90%)较为敏感,未检测到美罗培南耐药菌株;多重耐药菌株占比分别为87.99%和81.71%。8年间,两区分离菌株对多数抗菌药物的耐药率、多重耐药菌株占比和耐药谱数量整体呈下降趋势,松江区下降趋势更明显。结果表明,浦东新区和松江区畜禽源大肠埃希菌对部分抗菌药物的耐药率高且多重耐药情况严重,在减抗背景下,两区耐药水平均有缓解,松江区缓解程度更大。

关键词：大肠埃希菌耐药性兽用抗菌药减量化行动浦东新区松江区

CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription

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Cellular & Molecular Immunology 2021年第2期18卷 339-349页

作者： Yu Song Peishan Li Liping qin zhiliang Xu Baichun Jiang Chunhong Ma Changshun Shao Yaoqin Gong Key Laboratory of Experimental Teratology Ministry of EducationInstitute of Molecular Medicine and GeneticsSchool of Basic Medical SciencesShandong UniversityJinanShandongChina Key Laboratory of Experimental Teratology Ministry of EducationDepartment of ImmunologySchool of Basic Medical ScienceShandong UniversityJinanShandongChina State Key Laboratory of Radiation Medicine and Protection Institutes for Translational MedicineSoochow UniversitySuzhouJiangsuChina

Toll-like receptors (TLRs) play critical roles in innate immunity and inflammation. The molecular mechanisms by which TLR signaling is fine-tuned remain to be completely elucidated. Cullin 4B (CUL4B), which assembles the CUL4B-RING E3 ligase complex (CRL4B), has been shown to regulate diverse developmental and physiological processes by catalyzing monoubiquitination for histone modification or polyubiquitination for proteasomal degradation. Here, we identified the role of CUL4B as an intrinsic negative regulator of the TLR-triggered inflammatory response. Deletion of CUL4B in macrophages increased the production of proinflammatory cytokines and decreased anti-inflammatory cytokine IL-10 production in response to pathogens that activate TLR3, TLR4, or TLR2. Myeloid cell-specific Cul4b knockout mice were more susceptible to septic shock when challenged with lipopolysaccharide, polyinosinic-polycytidylic acid or Salmonella typhimurium infection. We further demonstrated that enhanced TLR-induced inflammatory responses in the absence of CUL4B were mediated by increased GSK3β activity. Suppression of GSK3β activity efficiently blocked the TLR-triggered increase in proinflammatory cytokine production and attenuated TLR-triggered death in Cul4b mutant mice. Mechanistically, CUL4B was found to negatively regulate TLR-triggered signaling by epigenetically repressing the transcription of Pten, thus maintaining the anti-inflammatory PI3K-AKT-GSK3β pathway. The upregulation of PTEN caused by CUL4B deletion led to uncontrolled GSK3β activity and excessive inflammatory immune responses. Thus, our findings indicate that CUL4B functions to restrict TLR-triggered inflammatory responses through regulating the AKT-GSK3β pathway.

关键词： CUL4B TLR signaling Transcriptional repression

尼莫地平对家兔实验性肠缺血再灌注损伤的保护作用

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西南国防医药 2001年第2期11卷 82-84页

作者：王鹏巨吴勤万勇尹致良成都军区总医院干部病房成都610083 成都军区总医院ICU 成都610083 成都军区总医院腹部外科成都610083

目的:观察尼莫地平对家兔实验性肠缺血再灌注后低血压、肠屏障功能障碍及小肠组织学损害的影响。方法:夹闭肠系膜上动脉90min后松夹,复制家兔肠缺血再灌注模型。动物分为a.假手术组,b.模型组,c.氢化可的松治疗组,d.尼莫地平治疗组。结果... 详细信息

目的:观察尼莫地平对家兔实验性肠缺血再灌注后低血压、肠屏障功能障碍及小肠组织学损害的影响。方法:夹闭肠系膜上动脉90min后松夹,复制家兔肠缺血再灌注模型。动物分为a.假手术组,b.模型组,c.氢化可的松治疗组,d.尼莫地平治疗组。结果:(1)再灌注后b,c,d组血压持续下降,但观察终点d组血压显著高于b,c组(P<0.01);(2)再灌注后b,c,d组心率持续减慢,以d组更为突出;(3)门静脉血内毒索含量a组显著低于b,c,d组(P<0.01),d组显著低于b,c组(P<0.01);(4)小肠病理组织学a组正常,b组呈重度损害;c、d组呈中度损害。结论:尼莫地平能减轻家兔肠缺血再灌注后低血压和小肠病理组织学损害,降低门静脉血内毒素含量,但同时有减慢心率的作用。

关键词：小肠缺血再灌注损伤钙通道阻滞剂尼莫地平治疗

基于改进YOLOv4深度学习的有雾海面船只识别

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应用科技 2023年第5期50卷 37-45页

作者：孙智文秦志亮彭若松马林伟马本俊刘雪芹赵杰臣青岛哈尔滨工程大学创新发展中心山东青岛266000 哈尔滨工程大学青岛创新发展基地山东青岛266000

为了提高有雾场景下船只检测识别的准确率,本文运用四分法计算求解大气光值实现对暗通道先验去雾算法的优化,引入空洞卷积方法和K-means++聚类算法改进YOLOv4算法,提出改进的暗通道先验去雾算法和改进YOLOv4深度学习的船只检测方法。通... 详细信息

为了提高有雾场景下船只检测识别的准确率,本文运用四分法计算求解大气光值实现对暗通道先验去雾算法的优化,引入空洞卷积方法和K-means++聚类算法改进YOLOv4算法,提出改进的暗通道先验去雾算法和改进YOLOv4深度学习的船只检测方法。通过与不同去雾算法和船只识别算法进行对比实验分析,改进后的方法更好地实现了海面船只的实时检测及分类识别。实验结果表明该方法解决了原去雾算法中去雾图像亮度偏暗等问题,提高了船只识别的准确率与实时性,对海上有雾环境条件下的船只实时检测研究具有一定的理论指导意义。

关键词：船舶检测船舶分类识别图像去雾暗通道先验去雾算法深度学习 YOLOv4算法 K-means++方法空洞卷积方法

Ensemble of single-atom catalysis and defect engineering in Cu_(1)/CeO_(2) nanozymes for tumor therapy

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Science China Chemistry 2023年第9期66卷 2590-2599页

作者： Hao-Xin Liu zhiliang Gao Han Yan Shan-qing Li Wei-Wei Wang Xuetao qin Hongning Sun Jiwei Cui Chun-Jiang Jia Key Laboratory of Colloid and Interface Chemistry Key Laboratory of Special Aggregated Materials of the Ministry of EducationSchool of Chemistry and Chemical EngineeringShandong UniversityJinan 250100China School of Materials and Environmental Engineering Chizhou UniversityChizhou 247000China

As a class of nanomaterials with natural enzyme-like characteristics, nanozymes have shown their great potential in various applications. Reducible metal oxides featured with defect structures, and single-atom catalysts with isolated metal sites are regarded as two of the most promising nanozymes. However, the strategies to construct highly performed nanozymes by combining these advantages are rarely reported. Herein, we report the coordination-unsaturated single-atomic Cu species supported on sintered CeO_(2), which combines the advantages of defect engineering and single-atom catalysis, exhibiting a largely enhanced peroxidase(POD)-like activity. The high-temperature calcination induces the transformation of inert Cu_(1)O_(4) species into coordination-unsaturated Cu_(1)O_(3) sites. This novel Cu_(1)O_(3) active sites with an unsaturated coordination work as a new type of defect sites to greatly activate the isolated Cu atoms and accelerate the dissociation of H_(2)O_(2) to form hydroxyl radicals(·OH). The obtained nanozyme with a high POD-like activity possesses low cytotoxicity, showing potential applications for the tumor inhibition in vitro and in vivo.

关键词： single-atom catalysis oxygen defect Cu/CeO_(2) peroxidase-like activity tumor inhibition

Integration of genetic virtual screening patterns and latent multivariate modeling techniques for QSAR optimization based on combinations and/or interactions between peptides and proteins

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Science China Chemistry 2008年第5期51卷 487-496页

作者： LI zhiliang TIAN FeiFei WU ShiRong YANG ShanBin YANG ShengXi ZHOU Yuan ZHANG QiaoXia qin RenHui MEI Hu CHEN Gang LI GenRong Key Laboratory for Systematic Chemobiomedical Science and Technology under Chongqing Municipality College of Chemistry and Chemical EngineeringChongqing UniversityChongqing 400044China State Key Laboratory for Chemobiosensors and Chemobiometrics under MOST Hunan UniversityChangsha 410012China Key Laboratory for Biomechanics and Biomedical Engineering under both Ministry of Education and Chongqing Municipality Col-lege of Life Science and BioengineeringChongqing UniversityChongqing 400044China Technology Centre for Life Sciences and Biotechnology Singapore Polytechnic500 Dover RoadSingapore 139651Singapore

Both the concept and the model of snug quantitative structure-activity relationship (QSAR) were proposed and developed for molecular design through constructing QSAR based on some known mode of receptor/ligand interactions. Many disadvantages of traditional models can be avoided by using the proposed method because the traditional models only determined upon molecular structural features in sample sets themselves. A genetic virtual screening of peptide/protein combinations (GVSPPC) is proposed for the first time by utilizing this idea to examine peptide/protein affinity activities. A genetic algorithm (GA) was developed for screening combinative targets with an interaction mode for virtual receptors. GVSPPC succeeds in disposing difficulties in rational QSAR, in order to search for the ligand/receptor interactions on conditions of unknown structures. Some bioactive oligo-/poly-peptide systems covering 58 angiotensin converting enzyme (ACE) inhibitors and 18 double site mutation residues in camel antibody protein cAb-Lys3 were investigated by GVSPPC with satisfactory results (R cu 2 > 0.91, Q cv 2 > 0.86, E RMS = 0.19?0.95), respectively, which demonstrates that GVSPPC is more interpretable in the ligand-receptor interaction than the traditional QSAR method.

关键词： genetic virtual screening of peptide-protein combination (GVSPPC) quantitative structure-activity relationship (QSAR) genetic algorithm (GA) partial least square (PLS) principal component analysis (PCA)

Effectiveness of switching to bictegravir/emtricitabine/tenofovir alafenamide in virologically suppressed people with HIV with historical drug resistance mutations

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Chinese Medical Journal 2024年第22期137卷 2758-2760页

作者： Ziwei Chang Hongyan Zhu Yiting Zhang Yaling Chen Jiahui Li Jiamin qin Yueping Zhu Hongxia Wei Yongfeng Yang Meiyin Zou Feng Qian zhiliang Hu Department of Infectious Disease The Second Hospital of NanjingNanjing University of Chinese MedicineNanjingJiangsu 210003China Clinical Research Center The Second Hospital of NanjingNanjing University of Chinese MedicineNanjingJiangsu 210003China Department of Infectious Diseases The Affiliated Infectious Diseases HospitalSuzhou Medical College of Soochow UniversitySuzhouJiangsu 215007China Department of Infectious Diseases Nantong Third People’s HospitalAffiliated Nantong Hospital 3 of Nantong UniversityNantongJiangsu 226006China

To the Editor:China provides free antiretroviral therapy(ART)for people with human immunodeficiency virus(HIV),typically using two nucleoside reverse transcriptase inhibitors(NRTIs)and one non-nucleoside reverse transcriptase inhibitor(NNRTI).This regimen has significantly reduced mortality and slowed HIV spread over the past 20 years.

关键词： mortality regimen resistance

消融Marshall韧带远段可降低交感神经活性减轻犬急性心房电重构

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中华心律失常学杂志 2017年第6期21卷 509-514页

作者：刘珊鲁志兵余小梅秦智亮罗达胡慧慧王晓莹马瑞松何文博何勃谢菁江洪武汉大学人民医院心内科武汉大学心血管病研究所 430060

目的探讨消融Marshall韧带远段对犬急性心房电重构的影响.方法通过左心耳电极发放1200次/min电刺激,2倍起搏阈值进行6 h快速心房起搏（6 h-RAP）建立急性心房电重构模型.采用随机数字表法将16只成年雄性杂种犬随机分为两组：第1组（n=... 详细信息

目的探讨消融Marshall韧带远段对犬急性心房电重构的影响.方法通过左心耳电极发放1200次/min电刺激,2倍起搏阈值进行6 h快速心房起搏（6 h-RAP）建立急性心房电重构模型.采用随机数字表法将16只成年雄性杂种犬随机分为两组：第1组（n=8）,6 h-RAP后立即消融Marshall韧带远段;第2组（n=8）,消融Marshall韧带远段后立即进行6 h-RAP.消融Marshall韧带远段前后记录心率变异性（HRV）,计算高频功率（HF,0.15～0.40 Hz）、低频功率（LF,0.04～0.15 Hz）以及低频/高频比值（LF/HF）.分别在基础状态、消融后和起搏每小时末测心房、心耳和肺静脉各部位的有效不应期（ERP）、心房颤动（房颤）的诱发窗口（WOV）.结果消融Marshall韧带远段后,HF明显升高[（27.37±12.19）nu对（44.48±18.17）nu,P=0.0001],LF和LF/HF明显降低[LF：（48.42±18.45）nu对（30.77±16.14）nu,P=0.0000;LF/HF：（2.31±1.52）对（0.95±0.93）,P=0.0001].第1组各部位ERP在快速起搏的前2 h显著缩短,在起搏第3～6 h ERP维持稳定,而ERP离散度和各部位WOV的总和（ΣWOV）逐渐增宽,房颤易诱发.消融Marshall韧带远段后各部位的ERP较6 h-RAP末明显延长,而ERP离散度和ΣWOV显著降低（P均〈0.05）.第2组消融Marshall韧带远段后,各部位的ERP较基线明显延长,ΣWOV明显减小.随后在6 h-RAP过程中ERP离散度和ΣWOV无明显变化.起搏过程中第1组和第2组ERP均缩短,但第2组的缩短程度小于第1组（P〈0.05）.结论消融Marshall韧带远段可减轻由6 h-RAP引起的急性心房电重构,其机制与降低心脏交感神经活性有关.

关键词： Marshall韧带射频消融心房颤动快速心房起搏

Ensemble of single-atom catalysis and defect engineering in Cu/CeOnanozymes for tumor therapy

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Science China Chemistry 2023年

作者： Haoxin Liu zhiliang Gao Han Yan Shanqing Li Weiwei Wang Xuetao qin Hongning Sun Jiwei Cui Chunjiang Jia Key Laboratory of Colloid and Interface Chemistry Key Laboratory of Special Aggregated Materials of the Ministry of Education School of Chemistry and Chemical Engineering Shandong University School of Materials and Environmental Engineering Chizhou University

As a class of nanomaterials with natural enzyme-like characteristics, nanozymes have shown their great potential in various applications. Reducible metal oxides featured with defect structures, and single-atom catalysts with isolated metal sites are regarded as two of the most promising nanozymes. However, the strategies to construct highly performed nanozymes by combining these advantages are rarely reported. Herein, we report the coordination-unsaturated single-atomic Cu species supported on sintered CeO2, which combines the advantages of defect engineering and single-atom catalysis, exhibiting a largely enhanced peroxidase(POD)-like activity. The high-temperature calcination induces the transformation of inert Cu1O4species into coordination-unsaturated Cu1O3sites. This novel Cu1O3active sites with an unsaturated coordination work as a new type of defect sites to greatly activate the isolated Cu atoms and accelerate the dissociation of H2O2to form hydroxyl radicals(·OH). The obtained nanozyme with a high POD-like activity possesses low cytotoxicity, showing potential applications for the tumor inhibition in vitro and in vivo.

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