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Relationship Between Root Chemical Signals and Grain Quality of Rice

Agricultural Sciences in China 2007年第1期6卷 47-57页

作者： YANG Jian-chang CHANG Er-hua ZHANG Wen-jie WANG Zhi-qin liU li-jun Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou University Yangzhou 225009 PR. China

This study was designed to elucidate the relationship between root chemical signals and the quality of rice. Various rice genotypes were used. Zeatin （Z）＋ zeatin riboside （ZR）, abscisic acid （ABA）, 1-aminocylopropane -1-carboxylic acid （ACC）, and organic acids in roots during grain filling and the appearance quality, cooking/eating quality were investigated. The correlations among them were analyzed. The results showed that Z ＋ ZR concentrations in the roots at mid- and lategrain-filling stages were significantly and positively correlated with the gel consistency and alkali spreading value （r = 0.72^＊ - 0.90^＊＊）, whereas negatively correlated with the amylose content （r = -0.68^＊ - -0.78^＊＊）. ABA concentrations in roots at mid- grain-filling stage were significantly and negatively correlated with the gel consistency and alkali spreading value （r = -0.90^＊＊-0.91^＊＊）, and positively correlated with the amylose content （r = 0.87^＊＊）. ACC concentrations in root exudates at mid-grain-filling stage were very significantly correlated with the percentage of chalky grains and chalkiness （r = 0.97^＊＊ - 0.98^＊＊）, and those at late-grain-filling stage Were significantly correlated with chalkiness and chalky size （r = 0.69^＊ - 0.96^＊＊）. The more the malic acid and succinic acid exuded from roots for a cultivar, the greater the breakdown values and the smaller the setback values in the starch profile, and the results were reversed for a cultivar with more tartaric acid and citric acid exuded from roots during the grain-filling period. The cultivar with more lactic acid in exudates had smaller gel consistency and alkali spreading values, but had greater amylose content. When roots were treated with exogenous ZR, ABA, and ACC during grain filling, effects of the chemicals on the rice quality were consistent with the relationships of the endogenous hormones （Z ＋ ZR, ABA, and ACC） with the quality indexes. Using rape cake as organic fertilizer can increase the concentrations of mali

关键词： rice root chemical signals appearance quality cooking and eating quality

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In Vitro Construction of Effective M1GS Ribozymes Targeting HCMV UL54 RNA Segments

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Acta Biochimica et Biophysica Sinica 2005年第3期37卷 210-214页

作者： Yun-Zhen SU Hong-Jian li Yue-qin li Hao-jun CHEN Dong-Sheng TANG Xin ZHANG Hong JIANG Tian-Hong ZHOU College of life Science and Technology,Jinan University,Guangzhou 510632,China College of life Science and Technology Jinan UniversityGuangzhou 510632 China

Seven sequence-specific ribozymes(M1GS RNAs)derived in vitro from the catalytic RNAsubunit of Escherichia coli RNase P and targeting the mRNAs transcribed by the UL54 gene encoding theDNA polymerase of human cytomegalovirus were screened from 11 ribozymes that were designed based onfour rules:(1)the NCCA-3′ terminal must be unpaired with the substrate;(2)the guide sequence(GS)mustbe at least 12 nt in length;(3)the eighth nucleotide must be U,counting from the site-1;and(4)around thecleavage site,the sites-1/+1/+2 must be U/G/C or C/G/*** investigation of the factors affecting thecleavage effect and the optimal ratio for M1GS/substrate was carried *** was determined that the optimalratio for M1GS/substrate was 2:1 and too much M1GS 1ed to substrate *** indicated *** M1GS that cleaved HCMV UL54 RNA segments in vitro were successfully designed and *** studies support the use of ribozyme M1GS as antisense molecules to silence HCMV mRNA in vitro,andusing the selection procedure as a general approach for the engineering of RNase P ribozymes.

关键词： ribonuclease P (RNase P) guide sequence HCMV DNA polymerase

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Water-requirement Characteristics and Water-saving Irrigation Indices of Dry-raised Rice Seedlings in Paddy Field

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Agricultural Sciences in China 2001年第1期 107-115页

作者： YANG Jian-chang WANG Wei WANG Zhi-qin liU li-jun DING Zhi-jia ZHU qing-sen Agricultural College Yangzhou University Yangzhou 225009 Agricultural College Yangzhou University Yangzhou 225009 Agricultural College Yangzhou University Yangzhou 225009 Agricultural College Yangzhou University Yangzhou 225009 Seed Station of Yiling Township Jiangdu County Agricultural College Yangzhou University Yangzhou 225009

The dry-raised seedlings (D-RS)of rice had obvious superiority in tillering and growth after transplanting. Especially under the condition of water-saving irrigation or low soil moisture, D-RS had more productive tillers, higher dry matter accumulation, larger grain-filling rate, slower senescence of leaves and stronger root activity during ripening, and higher grain yield, compared with the moist-raised seedlings (MRS). D-RS had smaller yield reduction than M-RS when subject to heavy water stress. The results suggested that D-RS had the ability to adapt to lower soil moisture in paddy field. Basedon the response of D-RS to soil moisture at each growth stage, the water-saving and high-yielding irrigation indices through controlling lowlimit soil water potential were proposed, i.e. soil water potential was - 15 - - 20 kPa from the recovery to the criticalleaf-age of productive tillering, - 20 - - 40 kPa from the critical leaf-age of productive tillering to secondary branch-differentiating stage, - 15 - - 25 kPa from secondary branch-differentiating stage to 20 days after heading and - 25 - - 35 kPa from 21 to 45 days after heading.

关键词： Rice Dry-raised seedlings Soil water potential Irrigation indices

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OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models

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World Journal of Gastroenterology 2008年第30期14卷 4800-4804页

作者： jun Shi Pin-Kang Wei Shen Zhang Zhi-Feng qin jun li Da-Zhi Sun Yan Xiao Zhi-Hong Yu Hui-Ming lin Guo-Jing Zheng Xiao-Mei Su Ya-lin Chen Yan-Fang liu ling Xu Department of Traditional Chinese Medicine Shanghai Changzheng Hospital Shanghai 200003 China

AIM： To establish nude mouse human gastric cancer orthotopic transplantation models using OB glue paste technique. METHODS： Using OB glue paste technique, orthtopic transplantation models were established by implanting SGC-7901 and NKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto-metastasis of the two models were observed. RESULTS： The success rates of orthotopic transplanration of the two models were 94.20% and 96%. The rates of hepatic metastasis, pulmonary metastasis, peritoneal metastasis, lymphocytic metastasis and splenic metastasis were 42.13% and 94.20%, 48.43% and 57.97%, 30.83% and 36.96%, 67.30% and 84.06%, and 59.75% and 10.53%, respectively. The occurrence of ascites was 47.80% and 36.96%. CONCLUSION： OB glue paste technique is easy to follow. The biological behaviors of the nude mouse human gastric cancer orthotopic transplantation models established with this technique are similar to the natural processes of growth and metastasis of human gastric cancer, and, therefore, can be used as an ideal model for experimental research of proliferative metastasis of tumors.

关键词： Gastric tumor Tumor transplantation Disease models Animal Nude mice

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Separation and Identification of Isotopes Produced from ^(20)Ne+Be Reaction by Radioactive Ion Beam line in Lanzhou

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Chinese Physics Letters 1998年第11期15卷 790-792页

作者： SUN Zhi-yu ZHAN Wen-long GUO Zhong-yan XIAO Guo-qing WANG Jin-chuan li Jia-xing JIANG Shan-hong MENG Xiang-wei qin li-jun WANG Quan-jin Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000

The separation and identification of isotopes emitted from the projectile fragmentation reaction of 80 MeV/u 20Ne on 9Be target were carried out by using the radioactive ion beam line in Lanzhou(RIBLL).Many proton rich isotopes are *** intensity and purity fit the theoretical *** separation and identification abilities of RIBLL A/ΔA>300 and Q/ΔQ>150,are obtained.

关键词： MeV/u separation purity

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Mechanism of reduction of albumin expression induced by lipopolysaccharide in rat hepatocytes

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中华医学杂志(英文版) 2005年第20期 25-32页

作者： WANG Xin-ying, li Wei-qin, LU jun, li Ning and li Jie-shou Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, China Department of Orthopaedics, Zhongda Hospital, Nanjing 210009, China

Background The severity of hypoalbuminemia has been sho wn to be related to morbidity and mortality in some critical illnesses, illustra ting the need for better understanding of molecular mechanism of hypoalbuminemia . lipopolysaccharide (LPS) is a key mediator inducing hypoalbuminemia in sepsis and septic shock. The present study was designed to identify if the reduction of albumin expression is directly induced by LPS and modulated by activated extr acellular signal-regulated protein kinase (ERK) and p38 mitogen-activated prot ein kinase (MAPK) in rat hepatocytes. Methods Primary rat hepatocytes were divided into five groups. In two of them, hepatocytes were treated with normal saline or 1 μg/ml LPS, th en albumin mRNA expression was observed at 0, 2, 8, 12 and 24 hours after treatm ent. In another group, hepatocytes were pretreated with 100, 40 or 20 μmol/L o f cycloheximide (CHX, an inhibitor of protein synthesis) for 30 minutes followed by 1 μg/ml LPS for 24 hours. Then the RNA was extracted from the cells for RT -PCR to detect the expression of albumin. The other two groups were administer ed 1 μmol/L, 10 μmol/L and 50 μmol/L of SB203580 (p38 MAPK inhibitor) or PD98 059 (ERK inhibitor) 30 minutes prior to 1 μg/ml LPS treatment. After 24 hours of LPS treatment, the supernatant was collected and assayed for albumin concentr ations. Data were analyzed by one-way analysis of variance, followed by the Ne wman-Keul test; a Pline. The reductio n did not occur until 24 hours after LPS treatment, and albumin mRNA decreased b y 30% approximately compared to the control group at 24 hours (0.587 vs 0.832, P=0.007). CHX could inhibit the decline of albumin mRNA induced by LPS an d the effect was correlated with the dose of CHX. The ERK inhibitor PD98059 cau sed a significant increase in LPS-induced albumin production

关键词： lipopolysaccharide · hepatocytes · albumin · hypoalbuminemia · signal transduction

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提升教师教育质量:德国教师教育见习制度发展新趋向

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教师教育研究 2015年第4期27卷 100-105页

作者：覃丽君西南大学教师教育学院重庆400715

德国教师教育见习制度在世界上独树一帜。进入21世纪以来,面对国内外各方面条件的发展与变化,为了提升教师教育质量,德国政府对其见习制度进行了调整,主要表现为扩展见习阶段准入条件、统一见习阶段服务时间、规范见习阶段的组成阶段及... 详细信息

德国教师教育见习制度在世界上独树一帜。进入21世纪以来,面对国内外各方面条件的发展与变化,为了提升教师教育质量,德国政府对其见习制度进行了调整,主要表现为扩展见习阶段准入条件、统一见习阶段服务时间、规范见习阶段的组成阶段及制定见习阶段教师教育标准,以上举措主要围绕德国教师教育质量整体提升、各州教师教育均衡发展及见习教师能力发展而展开。

关键词：德国教师教育见习制度发展趋向教育质量

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居民城郊游憩满意度实证研究——以山西省大同市民为例

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经济问题 2016年第4期 117-122页

作者：秦俊丽山西大同大学历史与旅游文化学院山西大同037009

对现有游憩满意度理论进行分析的基础上,结合游憩满意度模型,构建了市民游憩满意度指标体系。通过对657份有效样本的实证研究,运用均值分析比较大同市民城郊游憩满意度的高低,发现交通、资源和住宿满意度较高,而对服务设施、服务水平及... 详细信息

对现有游憩满意度理论进行分析的基础上,结合游憩满意度模型,构建了市民游憩满意度指标体系。通过对657份有效样本的实证研究,运用均值分析比较大同市民城郊游憩满意度的高低,发现交通、资源和住宿满意度较高,而对服务设施、服务水平及管理和购物满意度较低;通过单因素方差分析,得出游憩满意度在不同人口统计学特征的大同市民中的差异性。研究结论对大同市城郊休闲游憩系统的建设和完善,以及提高游憩经营管理水平等提供科学依据。

关键词：市民游憩满意度人口统计特征

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乡村振兴战略下休闲农业发展路径研究——以山西为例

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经济问题 2019年第2期 76-84页

作者：秦俊丽山西大同大学历史与旅游文化学院山西大同037009 北京大学城市与环境学院北京100871

休闲农业是利用农村景观资源和农业生产条件,发展观光、休闲、旅游的一种新型农业生产经营形态。在乡村振兴战略的大背景下,发展休闲农业可以深度开发农业资源潜力,调整农村产业结构,改善农业农村环境,增加农民收入。山西自然资源独特,... 详细信息

休闲农业是利用农村景观资源和农业生产条件,发展观光、休闲、旅游的一种新型农业生产经营形态。在乡村振兴战略的大背景下,发展休闲农业可以深度开发农业资源潜力,调整农村产业结构,改善农业农村环境,增加农民收入。山西自然资源独特,人文景观丰富多彩,具有发展休闲农业良好的资源条件和环境优势。在剖析休闲农业发展原则和动力模式基础上,分析了山西休闲农业总体发展布局、优势和存在的问题,探讨山西特色休闲农业发展模式,以期突破瓶颈、寻求机遇、科学规划,在乡村振兴战略的大背景下谋求休闲农业发展的最优路径。

关键词：休闲农业乡村振兴战略发展模式

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Multiple dependent reliability estimation of large range MEMS accelerometers in high temperature environment

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Journal of Measurement Science and Instrumentation 2019年第1期10卷 9-15页

作者： qin li HE Cheng YU li-xia WANG Wei qin li-jun School of Instrument and Electronics North University of ChinaTaiyuan 030051China School of Information and Communication Engineering North University of ChinaTaiyuan 030051China

In the past,only one performance parameter was considered in the reliability estimation of micro-electro-mechanical system (MEMS) accelerometers,resulting in a one-sided reliability evaluation. Aiming at the failure condition of large range MEMS accelerometers in high temperature environment,the corresponding accelerated degradation test is designed. According to the degradation condition of zero bias and scale factor,multiple dependent reliability estimation of large range MEMS accelerometers is carried out. The results show that the multiple dependent reliability estimation of the large range MEMS accelerometers can improve the accuracy of the estimation and get more accurate results.

关键词： large range MEMS accelerometers zero bias scale factor multiple dependent reliability estimation accelerated degradation test

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