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Synthesis of Sn nanocluster@carbon dots for photodynamic therapy application

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Chinese Chemical Letters 2021年第7期32卷 2287-2291页

作者： Xiaolong Hu Shuna Wang qinghua Luo Binghui Ge qin Cheng Chen Dong jiahui Xu Haizhen Ding Mingsheng Xu Antonio Claudio Tedesco Xin Huang Renquan Zhang Hong Bi School of Chemistry and Chemical Engineering Anhui Key Laboratory of Modem BiomanufacturingAnhui UniversityHefei 230601China Institutes of Physical Science and Information Technology Anhui UniversityHefei 230601China Department of Chemistry Center of Nanotechnology and Tissue Engineering-Photobiology and Photomedicine Research GroupFaculty of PhilosophySciences and Letters of Ribeirao PretoUniversity of Sao PauloRibeirao PretoSao Paulo 14040-901Brazil Department of Thoracic Surgery First Affiliated Hospital of Anhui Medical UniversityHefei 230032China

Recently,photodynamic therapy(PDT)has been extensively applied in clinical and coadjuvant treatment of various kinds of ***,the photosensitizer(PS)of PDT still lack of high production of singlet oxygen(^(1)O_(2)),low cytotoxicity and high ***,we propose a facile method for establishing a new core-shell structured Sn nanocluster@carbon dots(CDs)***,Sn^(4+)@S-CDs complex is synthesized using the sulfur-doped CDs(S-CDs)and SnCl4 as raw materials,and subsequently the new PS(Sn nanocluster@CDs)is obtained after vaporization of Sn4+@S-CDs ***,the obtained Sn nanocluster@CDs show an enhanced fluorescence as well as a higher ^(1)O_(2) quantum yield(QY)than *** high ^(1)O_(2) QY(58.3%)irradiated by the LED light(400-700 nm,40 mW/cm^(2)),induce the reduction of 4 T1 cancer cells viability by 25%.More intriguingly,no visible damage happens to healthy cells,with little impact on liver tissue due to renal excretion,both in vitro and in vivo experiments demonstrate that Sn nanocluster@CDs may become a promising PS,owning a high potential for application in PDT.

关键词： Sn nanocluster@CDs Photodynamic therapy Photosensitizer Biocompatibility Singlet oxygen

近区爆炸作用下砌体填充墙损伤破坏与动态响应的数值模拟

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振动与冲击 2021年第9期40卷 1-11页

作者：胡嘉辉吴昊方秦同济大学土木工程学院上海200092 陆军工程大学爆炸冲击防灾减灾国家重点实验室南京210007

民用建筑中广泛使用的砌体填充墙在爆炸荷载作用下产生的碎块严重威胁着建筑物内部人员和设备的安全。尤其是近区爆炸作用下,墙体会产生更为严重的局部破坏,碎片飞散速度更快,但已有的相关研究工作仍旧较少。基于已有普通黏土烧结砖填... 详细信息

民用建筑中广泛使用的砌体填充墙在爆炸荷载作用下产生的碎块严重威胁着建筑物内部人员和设备的安全。尤其是近区爆炸作用下,墙体会产生更为严重的局部破坏,碎片飞散速度更快,但已有的相关研究工作仍旧较少。基于已有普通黏土烧结砖填充墙的近区爆炸试验,开展了近区爆炸作用下砌体填充墙损伤破坏和动态响应的数值模拟,对比验证了Load Blast法、任意拉格朗日欧拉(arbitrary Lagrange-Eulerian,ALE)法和冲量法模拟近区爆炸荷载作用、预测墙体损伤破坏和动态响应的适用性。通过冲量法进一步讨论了相同比例距离下的爆炸距离、砌块砂浆界面黏结强度以及砌块材料模型的影响。结果表明:近区爆炸中,相同比例距离下,墙体损伤通常随爆炸距离增加而加剧,由局部破坏向整体坍塌破坏模式发展;墙体的抗爆性能随砌块砂浆黏结强度增强而得到强化,主要受剪切失效应力控制;MAT_BRITTLE_DAMAGE和MAT_WINFRITH_CONCRETE模型较MAT_SOIL_AND_FOAM模型预测效果更好。

关键词：砌体填充墙近区爆炸动态响应数值模拟比例距离

黄芪多糖对HD11鸡巨噬细胞转录组和代谢组的影响

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畜牧兽医学报 2024年第3期55卷 1290-1301页

作者：陈富斌徐国伟王磊刘琴冯海鹏张康郭志廷韩松伟刘佳惠古雪艳张景艳李建喜 Huub F.J.Savelkoul 中国农业科学院兰州畜牧与兽药研究所兰州730050 甘肃农业大学动物医学院兰州730070 荷兰瓦赫宁根大学细胞生物与免疫学教研组荷兰瓦赫宁根市

旨在通过分析黄芪多糖对鸡巨噬细胞HD11转录组和代谢组的作用,探讨黄芪多糖(Astragalus polysaccharide, APS)对鸡巨噬细胞HD11的免疫调节机制。设置空白对照组(CONT组)和APS处理组(A50组),其中,A50组用含50μg·mL^(-1)APS的完全培养... 详细信息

旨在通过分析黄芪多糖对鸡巨噬细胞HD11转录组和代谢组的作用,探讨黄芪多糖(Astragalus polysaccharide, APS)对鸡巨噬细胞HD11的免疫调节机制。设置空白对照组(CONT组)和APS处理组(A50组),其中,A50组用含50μg·mL^(-1)APS的完全培养液培养HD11细胞12 h;CONT组用完全培养液培养HD11细胞12 h。采用代谢组学与转录组学技术分析CONT组与A50处理组细胞中差异基因和差异代谢物,并进行2个组学的联合分析。结果显示:在A50处理组与CONT组的转录组分析比较中,共检测到差异基因845个,其中上调基因415个,下调基因430个;差异表达基因主要富集在细胞因子-细胞因子受体相互作用、Toll样受体信号通路、吞噬体、AGE-RAGE等信号通路,其中,细胞因子-细胞因子受体相互作用、Toll样受体信号2条通路最为显著性;在A50处理组与CONT组的代谢组分析比较中,共筛选出差异代谢物89个,其中,下调差异代谢物为70个,上调的差异代谢物为19个。在A50处理组与CONT组的转录与代谢组联合分析中共同富集到可信度排名前十的通路有嘌呤代谢、碳代谢、磷酸戊糖途径、谷胱甘肽代谢、组氨酸代谢、氨基糖和核苷酸糖代谢、α同亚麻酸代谢、ABC转运体、铁死亡和酪氨酸代谢。APS可能是通过TLR2受体信号通路、糖酵解和磷酸戊糖途径的代谢重编程,进而发挥对HD11细胞的免疫调节作用。

关键词：黄芪多糖巨噬细胞转录代谢

健脾化瘀解毒方治疗慢性萎缩性胃炎临床疗效回顾性研究

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中国中西医结合消化杂志 2024年第6期32卷 481-486页

作者：赵自明倪家慧李紫昕张伦李关英覃雪婷钟毅刘伟潘华峰弋焰广东省第二中医院(广东省中医药工程技术研究院广东省中医药研究开发重点实验室) 广州510095 广州中医药大学广州中医药大学第五临床医学院

目的:回顾性总结分析健脾化瘀解毒方(Jianpi Huayu Jiedu Decoction,SWF)对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)的临床疗效,对比观察SWF与胃复春治疗CAG的疗效异同。方法:回顾性收集2017年10月—2023年12月在广东省第二中... 详细信息

目的:回顾性总结分析健脾化瘀解毒方(Jianpi Huayu Jiedu Decoction,SWF)对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)的临床疗效,对比观察SWF与胃复春治疗CAG的疗效异同。方法:回顾性收集2017年10月—2023年12月在广东省第二中医院消化科门诊及住院的350例CAG患者的电子病历,分别随机选择符合标准的160例为治疗组(SWF),70例为对照组(胃复春),分析两组患者的萎缩、肠化和异型增生三大病理(分级、评分、疗效)情况、中医证候评分与疗效、临床疗效。结果:治疗后,治疗组患者的三大病理分级构成比、病理积分、病理积分差值、证侯评分和证侯评分差值均较对照组有显著改善(均P肠化>异型增生;治疗组的病理疗效、证侯疗效、临床疗效总有效率分别为90.0%、91.9%、83.8%,高于对照组的64.3%、77.1%和48.6%(均P<0.01)。结论:SWF可提高CAG患者的病理疗效、证候疗效和临床疗效,作用优于胃复春;治疗后SWF组CAG患者的病理疗效和证侯疗效总有效率相当。

关键词：慢性萎缩性胃炎健脾化瘀解毒方临床疗效

基于YOLOv3目标检测的秧苗列中心线提取方法

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农业机械学报 2020年第8期51卷 34-43页

作者：张勤王家辉李彬华南理工大学机械与汽车工程学院广州510641 华南理工大学自动化科学与工程学院广州510641

针对秧苗列中心线的检测结果易受到水田中的浮萍、蓝藻以及水面反射、风速、光照情况等自然条件影响的问题,提出一种基于YOLOv3目标检测的秧苗列中心线检测算法。基于透视投影计算提取图像的ROI(Region of interest)区域,采用ROI图像构... 详细信息

针对秧苗列中心线的检测结果易受到水田中的浮萍、蓝藻以及水面反射、风速、光照情况等自然条件影响的问题,提出一种基于YOLOv3目标检测的秧苗列中心线检测算法。基于透视投影计算提取图像的ROI(Region of interest)区域,采用ROI图像构建数据集,对YOLOv3模型进行训练,训练过程中通过减少YOLOv3模型的输出降低运算量,利用模型识别定位ROI内的秧苗,并输出其检测框,对同列秧苗的检测框进行自适应聚类。在对秧苗图像进行灰度化和滤波处理后,在同类检测框内提取秧苗SUSAN(Smallest univalue segment assimilating nucleus)角点特征,采用最小二乘法拟合秧苗列中心线。试验结果表明,该算法对于秧苗的不同生长时期,以及在大风、蓝藻、浮萍和秧苗倒影、水面强光反射、暗光线的特殊场景下均能成功提取秧苗列中心线,鲁棒性较好,模型的平均精度为91.47%,提取的水田秧苗列中心线平均角度误差为0.97°,单幅图像(分辨率640像素×480像素)在GPU下的平均处理时间为82.6 ms,能够满足视觉导航的实时性要求。为复杂环境下作物中心线的提取提供了有效技术途径。

关键词：水田秧苗中心线提取深度学习 ROI图像自适应检测框聚类

Effect of mitochondrial ALDH2 overexpression on high glucose-induced H9C2 cardiac cells pyroptosis

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Effect of mitochondrial ALDH2 overexpression on high glucose...

Herbal decoctosome is a novel form of medicine

2019中国生理学会学术年会暨张锡钧基金第十五届全国青年优秀生理学学术论文交流会及第十三届全国青年生理学工作者学术会议

作者：曹瑞平 Wang jiahui Fang Dian Gao qin Department of Physiology Bengbu Medical College

Purpose: To observe the occurrence of pyroptosis in high glucose induced H9 C2 myocardial cell injury and explore the changes and possible effects of mitochondrial ALDH2 on ***: The ALDH2 gene over-expressing H9 C2 cardiac cell line were constructed, after verifying the successful construction of the cell line through real-time PCR and western blot, the cells were divided into 6 groups: normal control(NG), lentivirus negative control(GFP), ALDH2 over-expression(ALDH2-GFP), high glucose(HG), HG + GFP and HG+ ALDH2-GFP *** mitochondrial ALDH2 activity was detected by ALDH2 kit, and the expressions of ALDH2 at mRNA and protein level were detected by real-time PCR and western *** cell viability was measured by Cell Counting Kit-8 colorimetric *** changes of mitochondrial oxidative stress were measured through Mitosox reagent, the protein expression levels of NLRP3, ASC and Caspase-1 were assessed by western ***: Compared with the NG group, in HG group and HG+GFP group, the cell viability, the red fluorescence intensities by Mitosox were significantly increased, ALDH2 activity, mRNA and protein expressions were decreased;in the HG+ALDH2-GFP group, compared with the HG group and HG+GFP group, the cell viability, red fluorescence intensity were weakened, ALDH2 activity and expression were *** the HG and HG + GFP groups, pyroptosis key factors-NLRP3,ASC, Caspase-1 protein expressions were increased compared with the NG *** levels of NLRP3, ASC and Caspase-1 protein in the HG+ALDH2-GFP group were significantly lower than that in the HG ***: Pyroptosis was involved in high glucose-induced cardiac cell injury, overexpression of ALDH2 may reduce mitochondrial ROS production, thereby inhibiting the activation of NLRP3 inflammasome and improving myocardial damage induced by high glucose stimulation.

关键词： diabetes NLRP3 inflammasome myocardial injury pyroptosis ALDH2

来源： cnki会议评论

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Science China(Life Sciences) 2019年第3期62卷 333-348页

作者： Xiaoyun Li Zhu Liang Jianchao Du Zhiqing Wang Song Mei Zhiqing Li Yan Zhao DANDan Zhao Yiming Ma Jun Ye Jiantao Xu Yu Zhao jiahui Chang Yuhao qin Lanlan Yu Chenxuan Wang Chengyu Jiang State Key Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Department of Biochemistry Peking Union Medical College Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Department of Biophysics and Structural Biology Peking Union Medical College

Traditionally, herbal medicine is consumed by drinking decoctions produced by boiling herbs with water. The functional components of the decoction are heat stable. Small RNAs(sRNAs) were reported as a new class of functional components in decoctions. However, the mechanisms by which sRNAs survive heat treatment of the decoction and enter cells are *** studies showed that plant-derived exosome-like nanoparticles(ELNs), which we call botanosomes, could deliver therapeutic reagents in vivo. Here, we report that heat-stable decoctosomes(ELNs) from decoctions have more therapeutic effects than the decoctions in vitro and demonstrate therapeutic efficacy in vivo. Furthermore, sRNAs, such as HJT-sRNA-m7 and PGY-sRNA-6, in the decoctosome exhibit potent anti-fibrosis and anti-inflammatory effects, respectively. Decoctosome is comprised of lipids, chemical compounds, proteins, and s RNAs. A medical decoctosome mimic is called bencaosome. A single lipid sphinganine(d22:0) identified in the decoctosome was mixed and heated with the synthesized sRNAs to form the simplest bencaosome. This simple bencaosome structure was identified by critical micelle concentration(cmc) assay that sRNAs coassembled with sphinganine(d22:0) to form the lipid layers of vesicles. The heating process facilitates co-assembly of sRNAs and sphinganine(d22:0) until a steady state is reached. The artificially produced sphinganine-HJT-sRNA-m7 and sphinganinePGY-sRNA-6 bencaosomes could ameliorate bleomycin-induced lung fibrosis and poly(I:C)-induced lung inflammation, respectively, following oral administration in mice. Our study not only demonstrates that the herbal decoctosome may represent a combinatory remedy in precision medicine but also provides an effective oral delivery route for nucleic acid therapy.

关键词： decoctosome bencaosome sphinganine

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基于信息融合的智能推料机器人设计与试验

农业机械学报 2023年第6期54卷 78-84,93页

作者：张勤任海林胡嘉辉华南理工大学机械与汽车工程学院广州510641

饲料的定期推送是奶牛饲喂过程中的重要环节,针对现有推料机器人功能单一,无法满足奶牛饲喂需求的问题,开发了奶牛智能推料机器人。构建奶牛、饲料和牛栏参照物识别与分割的YOLACT模型,融合掩膜图像、深度图与ORB-SLAM3定位信息,实现觅... 详细信息

饲料的定期推送是奶牛饲喂过程中的重要环节,针对现有推料机器人功能单一,无法满足奶牛饲喂需求的问题,开发了奶牛智能推料机器人。构建奶牛、饲料和牛栏参照物识别与分割的YOLACT模型,融合掩膜图像、深度图与ORB-SLAM3定位信息,实现觅食奶牛的快速定位与机器人导航信息的提取;基于信息融合提出智能推料算法,根据觅食奶牛的定位信息、投料时间信息、机器人的导航信息,自动选择工作模式,控制机器人沿着预定的轨迹,实现推料、集料送料、清料等多模式推料功能,满足奶牛个性化自由采食需求,提升饲料利用率。试验结果表明:觅食奶牛的位置识别定位精度为±0.1 m,奶牛识别率为100%,机器人导航精度为±0.8 cm,智能推料准确率为100%,算法运行速率为12 f/s,满足复杂环境下机器人智能推料的要求。

关键词：奶牛智能推料机器人实例分割动态物体剔除视觉SLAM

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饲喂辅助机器人的智能推料方法与试验研究

华南理工大学学报（自然科学版） 2022年第6期50卷 111-120页

作者：张勤胡嘉辉任海林华南理工大学机械与汽车工程学院广东广州510640

在中、大型牧场的奶牛饲喂中,现有的推料机器人功能单一,只能完成均匀推料功能,无法满足牛只的个性化采食需求。针对这个问题,文中提出了一种饲喂辅助机器人的智能推料方法。首先,引入二维码标牌作为牛颈枷的定位标签,基于YOLOv4深度学... 详细信息

在中、大型牧场的奶牛饲喂中,现有的推料机器人功能单一,只能完成均匀推料功能,无法满足牛只的个性化采食需求。针对这个问题,文中提出了一种饲喂辅助机器人的智能推料方法。首先,引入二维码标牌作为牛颈枷的定位标签,基于YOLOv4深度学习模型得到二维码标牌和牛头的检测框区域;然后,通过预处理和预测算法对二维码标牌的检测框区域进行实时识别与跟踪,并将二维码标牌与牛头进行匹配,确定觅食奶牛所在的采食颈枷位置;最后,根据牛-码位置匹配信息、余料分布信息控制机器人推板改变推料角度,实现个性化推料,以满足奶牛个体自由采食需求。实验结果表明:所提出的智能推料方法对二维码的识别率为96.0%;在二维码标牌连续丢失60帧的情况下,对二维码的跟踪预测精度在±2.85%以内;每帧图像在图形处理器中处理的时间为34.4 ms;智能送料的准确率为100%,满足牛舍复杂环境下机器人智能推料的实时性要求。

关键词：机器人智能推料奶牛饲喂二维码深度学习