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Sequence Comparison of MHCClassⅡβ(Exon 2)and Phylogenetic Relation-ship Between Poultry and Mammalian

Agricultural Sciences in China 2005年第4期4卷 299-309页

作者： XUmi-fu LIKui CHENGuo-hong qiangba-yang-zong MODe-lin LIChang-chun FANBin LIUBang LaboratoryofMolecularBiologyandAnimalBreeding CollegeofAnimalScienceandTechnologyHuazhongAgriculturalUniversityWuhan430070PR.China DepartmentofAnimalScienceandTechnology YangzhouUniversityYangzhou225009P.R.China DepartmentofAnimalHusbandryandVeterinary Collegeof4gricultureandAnimalHusbandryTibetanUniversityLinzhi860000P.R.China

A fragment spanning over exon 2 and intron 2 of major histocompatibility complex B-LB Ⅱ genes was amplified using PCR, cloned and sequenced in 13 individuals from eight Chinese indigenous chicken breeds and one introduced breed. Another 41 sequences of MHC class Ⅱ β from ten vertebrate species were cited from the NCBI GenBank. Thirteen new B-LB Ⅱ alleles were found in the chicken breeds sampled. Alignment of the exon 2 sequences revealed 91.1-97.8% similarity to each other within the chickens sampled, and the chickens shared 84.1-87.0% homology to Phasianus colchicus, 78.5-81.5% similarity to Coturnix japonica. The sequences in poultry showed 62.6-68.1% identity to HLA-DRB1, 50-61.5% similarity to DQB (HLA-, SLA- and H2-BB), 53.7-60% to HLA-DPB and 53.3-57.8% similarity to HLA-DOB. The frequency of nonsynonymous substitutions of nucleotide was higher than that of synonymous substitutions, and the frequencies of nonsynonymous and synonymous substitutions in poultry B-LB Ⅱ genes were lower than those observed in mammalian DRB1 and DQB1 genes. The deduced amino acid sequences of MHC class Ⅱ β1 domain exhibited extreme difference in conversed region and variable region patterns among the various species, but the two conserved cysteines forming disulfide-bond were shown consistent in poultry with that in mammalian species; and the carbohydrate attachment site was found more conserved in chicken, Homo sapiens, Bos taurus, Ovis aries and Capra hircus than in Sus scrofa and rodent animals. Compared with exon 2 of DQB1 genes of Homo sapiens, ruminant species and Sus scrofa, the differentia that the deletion of six nucleotides at position195 to 200 of exon 2 of DQB1 genes, and insertion of three nucleotides at position 247 to 249 of the exon 2 existed in rodent species were found, which led to the absence of three AA residues at position 65, 66, and 67 within β1 domain of DQB1 chain, and the insertion of one AA residue at position 85. The difference of the deletion of six nuc

关键词： Major histocompatibility complex Class Ⅱ β-chain Phylogenetic relationship Poultry Mammalian Nucleotide deletion

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Advances in self-assembly-based circularly polarized luminescent materials

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Science China Chemistry 2024年第10期67卷 3247-3257页

作者： Bing-Hao Liu yang zong Na Liu zong-Quan Wu State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012China The School of Pharmaceutical Sciences Jilin UniversityChangchun 130021China

Circularly polarized luminescent(CPL)materials have garnered attention for their diverse applications,including three-dimensional(3D)imaging,bio-imaging,chiral sensing,and other emerging ***,the fabrication of CPL-activated materials required the use of chiral luminescent small *** introduction of the idea of self-assembly has transformed the production of CPL-activated materials,providing new and promising opportunities for their *** utilizing self-assembly,it is now feasible to produce chiral nano-assemblies with CPL activity not only from chiral molecules but also from non-chiral substances and inorganic *** addition,self-assembly routes provide a way to increase the asymmetry factor,creating possibilities for additional refinement and adjustment of CPL material *** review offers a summary of the current progress and developments in the self-assembly of small molecules and polymers,with a specific emphasis on their capacity to create or enhance CPL in different *** aim of this review is to inspire interest in optical materials that are based on the principles of self-assembly.

关键词： circularly polarized luminescence photochemistry self-assembly chiral materials

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Analytical solution for the effective elastic properties of rocks with the tilted penny-shaped cracks in the transversely isotropic background

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Petroleum Science 2024年第1期21卷 221-243页

作者： Zheng-Qian Ma Xing-Yao Yin Zhao-Yun zong Yuan-Yuan Tan Ya-Ming yang National Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)Qingdao266580ShandongChina School of Geosciences China University of Petroleum(East China)Qingdao 266580ShandongChina Pilot National Laboratory for Marine Science and Technology(Qingdao) Qingdao266200ShandongChina

Seismic prediction of cracks is of great significance in many disciplines,for which the rock physics model is ***,up to now,multitudinous analytical models focus primarily on the cracked rock with the isotropic background,while the explicit model for the cracked rock with the anisotropic background is rarely investigated in spite of such case being often encountered in the ***,we first studied dependences of the crack opening displacement tensors on the crack dip angle in the coordinate systems formed by symmetry planes of the crack and the background anisotropy,respectively,by forty groups of numerical *** on the conclusion from the experiments,the analytical solution was derived for the effective elastic properties of the rock with the inclined penny-shaped cracks in the transversely isotropic ***,we comprehensively analyzed,according to the developed model,effects of the crack dip angle,background anisotropy,filling fluid and crack density on the effective elastic properties of the cracked *** analysis results indicate that the dip angle and background anisotropy can significantly either enhance or weaken the anisotropy degrees of the P-and SH-wave velocities,whereas they have relatively small effects on the SV-wave velocity ***,the filling fluid can increase the stiffness coefficients related to the compressional modulus by reducing crack compliance parameters,while its effects on shear coefficients depend on the crack dip *** increasing crack density reduces velocities of the dry rock,and decreasing rates of the velocities are affected by the crack dip *** comparing with exact numerical results and experimental data,it was demonstrated that the proposed model can achieve high-precision estimations of stiffness ***,the assumption of the weakly anisotropic background results in the consistency between the proposed model and Hudson's published theory for the or

关键词： Effective elastic property Tilted crack Transverse isotropy Analytical solution Crack opening displacement

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Predicting dynamic compressive strength of frozen-thawed rocks by characteristic impedance and data-driven methods

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第7期16卷 2591-2606页

作者： Shengtao Zhou zong-Xian Zhang Xuedong Luo Yifan Huang Zhi Yu Xiaowei yang Faculty of Engineering China University of GeosciencesWuhan430074China Oulu Mining School University of OuluOulu90570Finland Zijin School of Geology and Mining Fuzhou UniversityFuzhou350116China Department of Earth Science&Engineering Imperial College LondonLondonSW72AZUK

In cold regions,the dynamic compressive strength(DCS)of rock damaged by freeze-thaw weathering significantly influences the stability of rock ***,testing the dynamic strength under freeze-thaw weathering conditions is often both time-consuming and ***,this study considers the effect of characteristic impedance on DCS and aims to quickly determine the DCS of frozen-thawed rocks through the application of machine-learning ***,a database of DCS for frozen-thawed rocks,comprising 216 rock specimens,was *** external load parameters(freeze-thaw cycle number,confining pressure,and impact pressure)and two rock parameters(characteristic impedance and porosity)were selected as input variables,with DCS as the predicted *** research optimized the kernel scale,penalty factor,and insensitive loss coefficient of the support vector regression(SVR)model using five swarm intelligent optimization algorithms,leading to the development of five hybrid *** addition,a statistical DCS prediction equation using multiple linear regression techniques was *** performance of the prediction models was comprehensively evaluated using two error indexes and two trend indexes.A sensitivity analysis based on the cosine amplitude method has also been *** results demonstrate that the proposed hybrid SVR-based models consistently provided accurate DCS *** these models,the SVR model optimized with the chameleon swarm algorithm exhibited the best performance,with metrics indicating its effectiveness,including root mean square error(RMSE)﹦3.9675,mean absolute error(MAE)﹦2.9673,coefficient of determination(R^(2))﹦0.98631,and variance accounted for(VAF)﹦*** suggests that the chameleon swarm algorithm yielded the most optimal results for enhancing SVR ***,impact pressure and characteristic impedance emerged as the two most influential parameters in DCS *** research is

关键词： Freeze-thaw cycle Characteristic impedance Dynamic compressive strength Machine learning Support vector regression

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西藏藏猪肉营养成分的分析及评价

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营养学报 2019年第5期41卷 513-515页

作者：辜雪冬杨林池福敏罗章马长中强巴央宗西藏农牧学院

藏猪是中国特有的高原型地方猪种,主要生活在中国青藏高原2500~4300m海拔的农区和半农半林半牧区[1],在我国西藏、青海、四川、云南、甘肃等地有分布[2]。西藏藏猪主产区主要分布于林芝市、昌都市及那曲市嘉黎县,是西藏自治区重点建设... 详细信息

藏猪是中国特有的高原型地方猪种,主要生活在中国青藏高原2500~4300m海拔的农区和半农半林半牧区[1],在我国西藏、青海、四川、云南、甘肃等地有分布[2]。西藏藏猪主产区主要分布于林芝市、昌都市及那曲市嘉黎县,是西藏自治区重点建设的七个高原农产品产业带之一[3]。西藏藏猪生长极为缓慢,在放牧条件下,6月龄母猪体质量仅(15.34±2.15)kg,2岁以上成年母猪体重(33.04±2.76)kg,是我国目前生长速度最慢的猪品种之一[4]。国内研究主要为藏猪的遗传育种、生物适应性、疫病防治、藏猪加工等方面,对藏猪的营养成分研究偏少。本研究对西藏藏猪肉进行营养成分分析及评价,为合理开发利用西藏藏猪资源提供理论依据。

关键词：藏猪肉氨基酸矿物质脂肪酸

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Recombination and repeat-induced point mutation landscapes reveal trade-offs between the sexual and asexual cycles of Magnaporthe oryzae

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Journal of Genetics and Genomics 2024年第7期51卷 723-734页

作者： Xifang zong Yaxin Lou Mengshuang Xia Kunyang Zhao Jingxuan Chen Ju Huang Sihai yang Long Wang State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjingJiangsu 210000China State Key Laboratory for Crop Genetics and Germplasm Enhancement Bioinformatics CenterAcademy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjingJiangsu 210000China Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjingJiangsu 210000China

The fungal disease caused by Magnaporthe oryzae is one of the most devastating diseases that endanger many crops *** shows that sexual reproduction can be advantageous for fungal diseases as hybridization facilitates ***,the pervasive clonal lineages of *** observed in natural fields contradict this expectation.A better understanding of the roles of recombination and the fungi-specific repeat-induced point mutation(RIP)in shaping its evolutionary trajectory is essential to bridge this knowledge *** we systematically investigate the RIP and recombination landscapes in *** using a whole genome sequencing data from 252 population samples and 92 cross *** data reveal that the RIP can robustly capture the population history of ***,and we provide accurate estimations of the recombination and RIP rates across different *** ***,our results highlight a parent-of-origin bias in both recombination and RIP rates,tightly associating with their sexual potential and variations of effector *** bias suggests a critical trade-off between generating novel allelic combinations in the sexual cycle to facilitate host-jumping and stimulating transposon-associated diversification of effectors in the asexual cycle to facilitate host *** findings provide unique insights into understanding the evolution of blast fungus.

关键词： Magnaporthe oryzae Crossover Repeat-induced point mutation(RiP) Effectors Sexual reproduction

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An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

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Journal of Oceanology and Limnology 2024年第1期42卷 216-225页

作者： Zhongyi ZHANG Hang LIU Xiaohui PAN Yanan zong Leili FENG Lixian LIU Li GUO Guanpin yang College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institutes of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of ChinaQingdao 266003China

Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from *** genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional *** random insertional mutant library constructed using this method has become a method of forward genetics for gene ***,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous *** microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries *** this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was *** method will facilitate the genetic studies of ***,which should also be a reference for other microalgal species.

关键词： Nannochloropsis oceanica genetic transformation random insertional mutant library zeocin pretreatment forward genetics

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猪MYOZ2基因表达及其功能分析

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家畜生态学报 2017年第2期38卷 14-17页

作者：王亚非张健商鹏强巴央宗西藏大学农牧学院动物科学学院西藏林芝860000

试验以不同猪种为研究对象,检测MYOZ2基因在不同组织中的表达量,探究MYOZ2基因对猪生长性能的影响。结果表明,MYOZ2基因只在猪心脏和背最长肌中表达丰度高,在猪肌细胞组织中特异性表达。MYOZ2基因在生长速度较慢的小型猪(藏猪和滇南小耳... 详细信息

试验以不同猪种为研究对象,检测MYOZ2基因在不同组织中的表达量,探究MYOZ2基因对猪生长性能的影响。结果表明,MYOZ2基因只在猪心脏和背最长肌中表达丰度高,在猪肌细胞组织中特异性表达。MYOZ2基因在生长速度较慢的小型猪(藏猪和滇南小耳猪)背最长肌中表达显著低于生长速度较快的引进猪(大约克猪和长白猪)(P0.05)。研究结果显示,MYOZ2基因只在猪心肌和背最长肌中特异表达,且该基因在快速生长猪肌肉组织中表达高于慢速生长猪,可能通过钙调磷酸酶-NFAT信号通路调节肌肉的生长,为猪生长性状的重要候选基因。

关键词：猪 MYOZ2 基因表达肌肉生长

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A Bio-inspired Mutual-hook Strategy for the Soft Finger to Improve Load-bearing Capacity and Grasping Stability

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Journal of Bionic Engineering 2024年第3期21卷 1290-1304页

作者： Jie Huang Lingjie Gai Xiaofeng zong Yunquan Li School of Automation China University of GeosciencesWuhan430074China Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex System Wuhan430074China The Shien-Ming Wu School of Intelligent Engineering South China University of TechnologyGuangzhou510641China

Soft grippers have great potential applications in daily life,since they can compliantly grasp soft and delicate ***,the highly elastic fingers of most soft grippers are prone to separate from each other while grasping objects due to their low stiffness,thus reducing the grasping stability and load-bearing *** tackle this problem,inspired from the venus flytrap plant,this work proposes a mutual-hook mechanism to restrain the separation and improve the grasping performance of soft *** novel soft gripper design consists of three modules,a soft finger-cot,two Soft Hook Actuators(SHAs)and two sliding ***,the soft finger-cot covers on the soft finger,increasing the contact area with the target object,two SHAs are fixed to the left and right sides of the finger-cot,and the sliding mechanisms are designed to make SHAs stretch *** demonstrate that the proposed design can restrain the separation of soft fingers substantially,and the soft fingers with the finger-cots can grasp objects three times heavier than the soft fingers without the proposed *** proposed design can provide invaluable insights for soft fingers to restrain the separation while grasping,thus improving the grasping stability and the load-bearing capacity.

关键词： Soft robotics Soft bionic finger-cot Soft hook actuator Grasping stability Load-bearing capacity

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Data-driven active vibration control for helicopter with trailing-edge flaps using adaptive dynamic programming

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Chinese Journal of Aeronautics 2024年第6期37卷 151-166页

作者： Yu CHEN Qun zong Xiuyun ZHANG Jinna LI School of Electrical and Information Engineering Tianjin UniversityTianjin 300072China School of Information and Control Engineering Liaoning Petrochemical UniversityFushun 113001China

The helicopter Trailing-Edge Flaps(TEFs)technology is one of the recent hot topics in morphing wing *** employing controlled deflection,TEFs can effectively reduce the vibration level of ***,designing specific vibration reduction control methods for the helicopters equipped with trailing-edge flaps is of significant practical *** paper studies the optimal control problem for helicopter-vibration systems with TEFs under the framework of adaptive dynamic programming combined with Reinforcement Learning(RL).Time-delay and disturbances,caused by complexity of helicopter dynamics,inevitably deteriorate the control performance of vibration *** solve this problem,a zero-sum game formulation with a linear quadratic form for reducing vibration of helicopter systems is presented with a virtual *** this context,an off-policy reinforcement learning algorithm is developed to determine the optimal control *** algorithm utilizes only vertical vibration load data to achieve a policy that reduces vibration,attains Nash equilibrium,and addresses disturbances while compensating for time-delay without knowledge of the dynamics of the helicopter *** effectiveness of the proposed method is demonstrated in a virtual platform.

关键词： Active control Adaptive dynamic program-ming Helicopter rotor Vibration control Zero-sum game

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