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银对几种藻类的毒性浓度及其积累

南京大学学报（自然科学版） 1989年第3期25卷 132-138页

作者：钱凯先洪英南京大学生物系蠹南大学生物系

银对水生生物是一种有毒金属.本文以不同浓度的AgNO_3加入培养液中,通过对藻类生长的测定研究了银对三种常贝藻类的毒性浓度。1-2.5ppm的银对球衣藻和斜生栅藻是安全浓度范围,当银增至10ppm时,这两种藻类被明显致毒,生长繁殖被完全抑止... 详细信息

银对水生生物是一种有毒金属.本文以不同浓度的AgNO_3加入培养液中,通过对藻类生长的测定研究了银对三种常贝藻类的毒性浓度。1-2.5ppm的银对球衣藻和斜生栅藻是安全浓度范围,当银增至10ppm时,这两种藻类被明显致毒,生长繁殖被完全抑止.颤藻在10~3ppm银浓度中生长正常,对银具有较大的耐性,同时,以原子吸收光谱测定证明每0.2克(干重)颤藻可从这一浓度的含银水体中吸收10.17微克银,对银具有一定的积累能力.本文试验结果可供考虑含银水质排放标准、含银水体的生物净化及银的生物回收可能性之参考.

关键词：银球衣藻毒性浓度斜生栅藻藻

C_(60)对DNA光动力学作用的FT-Raman光谱研究

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中国激光 1998年第7期25卷 661-666页

作者：严庆丰钱凯先李文铸浙江大学生物科学与技术系

采用傅里叶变换激光Ｒａｍａｎ光谱术从分子水平研究了富勒烯Ｃ６０对小牛胸腺ＤＮＡ的光动力学作用。结果表明光激发Ｃ６０对ＤＮＡ各组分基团均有不同程度的损伤，其中ＤＮＡ的空间构像破坏显著，腺嘌呤次之，骨架磷酸基团、脱氧核糖... 详细信息

采用傅里叶变换激光Ｒａｍａｎ光谱术从分子水平研究了富勒烯Ｃ６０对小牛胸腺ＤＮＡ的光动力学作用。结果表明光激发Ｃ６０对ＤＮＡ各组分基团均有不同程度的损伤，其中ＤＮＡ的空间构像破坏显著，腺嘌呤次之，骨架磷酸基团、脱氧核糖、嘧啶环等也遭到破坏并导致ＤＮＡ链断裂。这种结果并非由于Ｃ６０与ＤＮＡ分子之间直接的电子相互作用，而是通过单线态氧（１Ｏ２）或·ＯＨ。

关键词： DNA 激光拉曼光谱术光动力学作用富勒烯碳60

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对Folin-酚法测定藻类蛋白质的改进

海洋与湖沼 1989年第2期20卷 192-196页

作者：钱凯先王澍生浙江大学生物科学与技术系北京蔬菜研究中心

在实验室中用以测定藻类蛋白质的方法很多,其中,Folin-酚法是应用最广泛的一种微量测定方法。该法综合了Folin和双缩脲法的优点,其所显的颜色比酚试剂单独使用时增加3—15倍,而灵敏度比双缩脲法增加近100倍。但是,Folin-酚法在测定藻类... 详细信息

在实验室中用以测定藻类蛋白质的方法很多,其中,Folin-酚法是应用最广泛的一种微量测定方法。该法综合了Folin和双缩脲法的优点,其所显的颜色比酚试剂单独使用时增加3—15倍,而灵敏度比双缩脲法增加近100倍。但是,Folin-酚法在测定藻类蛋白质时存在着样品的蛋白质提取问题,藻类细胞一般具有坚厚的细胞壁。

关键词： Folin-酚法藻类蛋白质蛋白质测定

C_(60)对体外培养癌细胞的光致作用研究

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生物化学与生物物理进展 1997年第3期24卷 237-241页

作者：钱凯先严庆丰黄文栋李文铸浙江大学生物科学与技术系杭州310027 浙江大学物理学系杭州310027

研究结果表明用C60 磷脂酰胆碱与体外培养的HeLa细胞融合后 ,以 4 0 0 0lx光强度激发C60(C60 浓度 2 0mg/L)对HeLa细胞具有显著的光致杀伤作用 .生化测定证实光激发C60 导致膜蛋白巯基含量减少、膜脂过氧化、丙二醛含量增高 ,SDS PAGE... 详细信息

研究结果表明用C60 磷脂酰胆碱与体外培养的HeLa细胞融合后 ,以 4 0 0 0lx光强度激发C60(C60 浓度 2 0mg/L)对HeLa细胞具有显著的光致杀伤作用 .生化测定证实光激发C60 导致膜蛋白巯基含量减少、膜脂过氧化、丙二醛含量增高 ,SDS PAGE证明膜蛋白交联 ,荧光偏振显示膜流动性降低 ,电镜超微膜结构破坏 ,经MTT法检测 ,大部分细胞死亡 .C60 的强烈光致作用证实了Arbogast等认为光激发C60 可产生单线态氧的观态 .

关键词：富勒烯癌细胞光致作用物理化学生物学

Interannual variability of boreal summer intraseasonal oscillation over the northwestern Pacific influenced by the Pacific Meridional Mode

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 6-11页

作者： Haoyu Zhou Pang-Chi Hsu Lin Chen Yitian qian Key Laboratory of Meteorological Disaster Ministry of Education/Joint International Research Laboratory of Climate and Environmental Change/Collaborative Innovation Center on Forecast and Evaluation of Meteorological DisastersNanjing University of Information Science and TechnologyNanjingChina

During the boreal summer,intraseasonal oscillations exhibit significant interannual variations in intensity over two key regions:the central-western equatorial Pacific(5°S-5°N,150°E-150°W)and the subtropical Northwestern Pacific(10°-20°N,130°E-175°W).The former is well-documented and considered to be influenced by the ENSO,while the latter has received comparatively less attention and is likely influenced by the Pacific Meridional Mode(PMM),as suggested by partial correlation analysis *** elucidate the physical processes responsible for the enhanced(weakened)intraseasonal convection over the subtropical northwestern Pacific during warm(cold)PMM years,the authors employed a moisture budget *** findings reveal that during warm PMM years,there is an increase in summer-mean moisture over the subtropical northwestern *** increase interacts with intensified vertical motion perturbations in the region,leading to greater vertical moisture advection in the lower troposphere and consequently resulting in convective *** a process is pivotal in amplifying intraseasonal convection *** observational findings were further verified by model experiments forced by PMM-like sea surface temperature patterns.

关键词： Boreal summer intraseasonal oscillation Interannual variability Pacific Meridional Mode Moisture budget analysis

Recent advances in exploring new blood-based biomarkers for the early diagnosis of gastric cancer

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Science China Chemistry 2025年第1期 35-45页

作者： Xinyu Peng qian Ma Da Han School of Life Science Shanghai University Institute of Molecular Medicine Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine State Key Laboratory of Oncogenes and Related Genes Renji Hospital School of Medicine Shanghai Jiao Tong University Department of Research and Development Intellinosis Biotechnologies Co.Ltd.

Gastric cancer(GC) is a prevalent and lethal malignancy worldwide, and the 5-year survival rate is less than 30%. The absence of distinctive symptoms often leads to late-stage diagnosis, contributing to a grim prognosis. Early detection is pivotal for improving the survival rates and outcomes of GC patients. Traditional diagnostic methods, including gastroscopy and histopathological examination, are restricted in the early diagnosis of GC. Conventional protein biomarkers, such as carcinoembryonic antigen(CEA), carbohydrate antigen 19-9(CA19-9), and carbohydrate antigen 72-4(CA72-4), exhibit insufficient sensitivity and specificity for GC screening, which diminishes their clinical value. Recently, novel circulating biomarkers, such as circulating tumor cells(CTCs), circulating tumor cell DNA(ct DNA), and circulating free RNA(cf RNA), have emerged as promising candidates in the burgeoning field of liquid biopsy due to their superior sensitivity and specificity. This review focuses on the latest research on blood-based biomarkers for GC diagnosis and examines the clinical potentials and challenges associated with these emerging biomarkers.

关键词：

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The pivotal role of microglia in injury and the prognosis of subarachnoid hemorrhage

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Neural Regeneration Research 2025年第7期20卷 1829-1848页

作者： Wenjing Ning Shi Lv qian Wang Yuzhen Xu Department of Rehabilitation The Second Affiliated Hospital of Shandong First Medical UniversityTaianShandong ProvinceChina Department of Central Laboratory The Affiliated Taian City Central Hospital of Qingdao UniversityTaianShandong ProvinceChina

Subarachnoid hemorrhage leads to a series of pathological changes,including vascular spasm,cellular apoptosis,blood–brain barrier damage,cerebral edema,and white matter ***,which are the key immune cells in the central nervous system,maintain homeostasis in the neural environment,support neurons,mediate apoptosis,participate in immune regulation,and have neuroprotective *** evidence has shown that microglia play a pivotal role in the pathogenesis of subarachnoid hemorrhage and affect the process of injury and the prognosis of subarachnoid ***,microglia play certain neuroprotective roles in the recovery phase of subarachnoid *** approaches aimed at modulating microglia function are believed to attenuate subarachnoid hemorrhage *** provides new targets and ideas for the treatment of subarachnoid ***,an in-depth and comprehensive summary of the role of microglia after subarachnoid hemorrhage is still *** review describes the activation of microglia after subarachnoid hemorrhage and their roles in the pathological processes of vasospasm,neuroinflammation,neuronal apoptosis,blood–brain barrier disruption,cerebral edema,and cerebral white matter *** also discusses the neuroprotective roles of microglia during recovery from subarachnoid hemorrhage and therapeutic advances aimed at modulating microglial function after subarachnoid ***,microglia in subarachnoid hemorrhage are targeted with TLR inhibitors,nuclear factor-κB and STAT3 pathway inhibitors,glycine/tyrosine kinases,NLRP3 signaling pathway inhibitors,Gasdermin D inhibitors,vincristine receptorαreceptor agonists,ferroptosis inhibitors,genetic modification techniques,stem cell therapies,and traditional Chinese ***,most of these are still being evaluated at the laboratory *** clinical studies and data on subarachnoid hemorrhage are required to improve the treatment of subarachnoid hemor

关键词： apoptosis blood–brain barrier brain edema microglia neuroinflammation neuron neuroprotection subarachnoid hemorrhage vasoconstriction white matter injury

Epigenetic regulation of the inflammatory response in stroke

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Neural Regeneration Research 2025年第11期20卷 3045-3062页

作者： Jingyi Liang Fei Yang Zixiao Li qian Li School of Basic Medical Sciences Capital Medical UniversityBeijingChina Department of Neurobiology School of Basic Medical SciencesCapital Medical UniversityBeijingChina Laboratory for Clinical Medicine Beijing Key Laboratory of Neural Regeneration and RepairCapital Medical UniversityBeijingChina Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina National Center for Healthcare Quality Management in Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Chinese Institute for Brain Research BeijingChina Research Unit of Artificial Intelligence in Cerebrovascular Disease Chinese Academy of Medical SciencesBeijingChina Beijing Engineering Research Center of Digital Healthcare for Neurological Diseases BeijingChina Department of Biochemistry and Molecular Biology School of Basic Medical SciencesCapital Medical UniversityBeijingChina Beijing Key Laboratory of Cancer Invasion and Metastasis Research Capital Medical UniversityBeijingChina

Stroke is classified as ischemic or hemorrhagic,and there are few effective treatments for either *** mechanisms play a critical role in secondary brain injury following a stroke,which manifests as cytokine release,blood–brain barrier disruption,neuronal cell death,and ultimately behavioral *** the inflammatory response has been shown to mitigate this cascade of events in experimental stroke ***,in clinical trials of anti-inflammatory agents,longterm immunosuppression has not demonstrated significant clinical benefits for *** may be attributable to the dichotomous roles of inflammation in both tissue injury and repair,as well as the complex pathophysiologic inflammatory processes in *** acute harmful inflammatory responses or inducing a phenotypic shift from a pro-inflammatory to an anti-inflammatory state at specific time points after a stroke are alternative and promising therapeutic *** agents that can modulate inflammation requires a detailed understanding of the inflammatory processes of ***,epigenetic reprogramming plays a crucial role in modulating post-stroke inflammation and can potentially be exploited for stroke *** this review,we summarize current findings on the epigenetic regulation of the inflammatory response in stroke,focusing on key signaling pathways including nuclear factor-kappa B,Janus kinase/signal transducer and activator of transcription,and mitogen-activated protein kinase as well as inflammasome *** also discuss promising molecular targets for stroke *** evidence to date indicates that therapeutic targeting of the epigenetic regulation of inflammation can shift the balance from inflammation-induced tissue injury to repair following stroke,leading to improved post-stroke outcomes.

关键词： DNA methylation histone modification intracerebral hemorrhage ischemic stroke neuroinflammation neuroprotection non-coding RNA RNA methylation subarachnoid hemorrhage treatment

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黑曲霉菊糖酶的分离纯化及其活性测定

微生物学通报 1998年第4期25卷 195-198页

作者：董强董云玲钱凯先于洪琴马庆恒山东省医科院基础医学研究所济南250062 山东省计划生育研究所浙江大学生命科学学院

从一株黑曲霉p319的菊芋培养液中，采用硫酸铵盐析、SephadexG-25、DEAE-cellulose-52、SephadexG-100柱层析等方法，分离纯化得到三个菊糖酶组份EI、EII、EⅢ，三者经聚丙烯酰胺凝胶电泳验证均达到均一。并对其活性进行了初步测定，三... 详细信息

从一株黑曲霉p319的菊芋培养液中，采用硫酸铵盐析、SephadexG-25、DEAE-cellulose-52、SephadexG-100柱层析等方法，分离纯化得到三个菊糖酶组份EI、EII、EⅢ，三者经聚丙烯酰胺凝胶电泳验证均达到均一。并对其活性进行了初步测定，三者比活分别达到29．8u／mg，4．9u／mg，1．2u／mg。

关键词：菊芋菊糖菊糖酶黑曲霉分离纯化活性