检索结果-南通市图书馆

The transcriptional regulators of virulence for Pseudomonas aeruginosa:Therapeutic opportunity and preventive potential of its clinical infections

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2023年第5期10卷 2049-2063页

作者： Xiaolong Shao Chunyan Yao Yiqing Ding Haiyan Hu guoliang qian Mingliang He Xin Deng College of Plant Protection Laboratory of Plant ImmunityKey Laboratory of Integrated Management of Crop Diseases and PestsNanjing Agricultural UniversityNanjingJiangsu 210095China Department of Biomedical Sciences City University of Hong KongKowloon TongHong Kong SARChina School of Pharmaceutical Sciences Sun Yat-Sen UniversityUniversity TownGuangzhouGuangdong 510006China Tung Biomedical Sciences Centre City University of Hong KongKowloon TongHong Kong SARChina Shenzhen Research Institute City University of Hong KongShenzhenGuangdong 518057China

In Pseudomonas aeruginosa(***),transcription factors(TFs)are important mediators in the genetic regulation of adaptability and pathogenicity to respond to multiple environmental stresses and host *** *** genome harbours 371 putative TFs;of these,about 70 have been shown to regulate virulence-associated phenotypes by binding to the promoters of their target *** the past three decades,several techniques have been applied to identify TF binding sites on the *** genome,and an atlas of TF binding patterns has been *** virulence-associated regulons of TFs show complex crosstalk in ***'s regulatory *** this review,we summarise the recent literature on TF regulatory networks involved in the quorum-sensing system,biofilm formation,pyocyanin synthesis,motility,the type III secretion system,the type VI secretion system,and oxidative stress *** discuss future perspectives that could provide insights and targets for preventing clinical infections caused by *** based on the global regulatory network of transcriptional regulators.

关键词： Crosstalk Pseudomonas aeruginosa Regulatory network Transcriptional regulators Virulence

Unlocking the bacterial contact-dependent antibacterial activity to engineer a biocontrol alliance of two species from natural incompatibility to artificial compatibility

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Stress Biology 2021年第1期1卷 230-242页

作者： qianhua Wu Bozhen Wang Xi Shen Danyu Shen Bingxin Wang Qinggang Guo Tao Li Xiaolong Shao guoliang qian College of Plant Protection Laboratory of Plant ImmunityKey Laboratory of Integrated Management of Crop Diseases and PestsNanjing Agricultural UniversityNo.1 WeigangNanjingJiangsu 210095People’s Republic of China Institute of Plant Protection Hebei Academy of Agricultural and Forestry SciencesIntegrated Pest Management Center of Hebei ProvinceKey Laboratory of IPM on Crops in Northern Region of North ChinaMinistry of Agriculture and Rural Affairs of ChinaBaoding 071000People’s Republic of China Shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesShanghai 200241People’s Republic of China

Plant growth-promoting rhizobacteria(PGPR)contain various biocontrol bacteria with broad-spectrum antimicrobial activity,and their single species has been extensively applied to control crop *** development of complex biocontrol community by mixing two or more PGPR members together is a promising strategy to enlarge the efficacy and scope of ***,an effective method to assess the natural compatibility of PGPR members has not yet been established to ***,we developed such a tool by using the bacterial contactdependent antibacterial activity(CDAA)as a *** showed that the CDAA events are common in two-species interactions in the four selected representative PGPRs,represented by the incompatible interaction of Lysobacter enzymogenes strain OH11(OH11)and Lysobacter antibioticus strain OH13(OH13).We further showed that the CDAA between OH11 and OH13 is jointly controlled by a contact-dependent killing device,called the type IV secretion system(T4SS).By deleting the respective T4SS synthesis genes,the T4SS in both strains was co-inactivated and this step unlocked their natural CDAA,resulting in an engineered,compatible mutant alliance that co-displayed antibacterial and antifungal ***,this study reveals that releasing bacterial CDAA is effective to rationally engineer the biocontrol community.

关键词： PGPR Biocontrol Contact-dependent antibacterial activity T4SS Engineering

Identification of atypical T4SS effector proteins mediating bacterial defense

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

mLife 2023年第3期2卷 295-307页

作者： Xi Shen Zixiang Yang Zihan Li Dan Xiong Jinxing Liao Weimei He Danyu Shen Xiaolong Shao Ben Niu Yongxing He Yong-Gui Gao guoliang qian State Key Laboratory of Biological Interactions and Crop Health Key Laboratory of Integrated Management of Crop Diseases and PestsCollege of Plant ProtectionNanjing Agricultural UniversityNanjingChina College of Life Science Northeast Forestry UniversityHarbinChina Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations Lanzhou UniversityLanzhouChina School of Biological Sciences Nanyang Technological UniversitySingaporeSingapore

To remain competitive,proteobacteria use various contact-dependent weapon systems to defend against microbial *** bacterial-killing type IV secretion system(T4SS)is one such powerful *** commonly controls the killing/competition between species by secreting the lethal T4SS effector(T4E)proteins carrying conserved XVIPCD domains into competing *** this study,we sought knowledge to understand whether the bacterial-killing T4SS-producing bacteria encode T4E-like proteins and further explore their biological *** achieve this,we designed a T4E-guided approach to discover T4E-like proteins that are designated as atypical ***,this approach required scientists to perform simple BlastP search to identify T4E homologs that lack the XVIPCD domain in the genomes of T4SS-producing *** homologous genes were then screened in Escherichia coli to identify antibacterial candidates(atypical T4Es)and their neighboring detoxification proteins,followed by testing their gene cotranscription and validating their physical *** this approach,we did discover two atypical T4E proteins from the plant-beneficial Lysobacter enzymogenes and the phytopathogen Xanthomonas *** also provided substantial evidence to show that the atypical T4E protein Le1637-mediated bacterial defense in interspecies interactions between *** and its ***,the newly designed T4E-guided approach holds promise for detecting functional atypical T4E proteins in bacterial cells.

关键词： atypical effectors defense immunity protein T4SS toxic

A unique role of the pyrimidine de novo synthesis enzyme ODCase in Lysobacter enzymogenes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第9期23卷 3066-3077页

作者： Mingming Yang Yunxiao Tan Jiabing Ma Yingjia Zhao Xia Yan Nana Wang Pingping Wang Jiaqi Tan Suilong Ai Xiaofei Liang Bangshuai Chang Obadah E.A.Yousif Chao Zhao Bo Wang guoliang qian Lili Huang State Key Laboratory for Crop Stress Resistance and High-Efficiency Production Northwest A&F UniversityYangling 712100China College of Plant Protection Northwest A&F UniversityYangling 712100China College of Life Sciences Northwest A&F UniversityYangling 712100China College of Natural Resources and Environment Northwest A&F UniversityYangling 712100China Shaanxi Provincial Tobacco Corporation of China National Tobacco Corporation Xi’an 710061China Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjing 210014China College of Plant Protection Laboratory of Plant ImmunityNanjing Agricultural UniversityNanjing 210095China

Bacterial species of the genus Lysobacter are environmentally ubiquitous with strong antifungal biocontrol ***-stable antifungal factor(HSAF)secreted by the biocontrol bacterium Lysobacter enzymogenes OH11 has broad-spectrum and highly efficient antifungal *** the biosynthetic regulations of HSAF would lay an important foundation for strain engineering toward improved HSAF *** this work,we demonstrate that Le0752,an orotidine-5´-phosphate decarboxylase enzyme(ODCase)catalyzing a pivotal step of the UMP de novo biosynthesis pathway,is vital for HSAF-mediated antimicrobial activities and growth of *** OH11,but not for twitching *** gene regulates the production of HSAF by affecting the expression of lafB,a key gene in the HSAF biosynthesis operon,through the transcription factor ***,bioinformatics analysis revealed that Le0752 belongs to the Group III ODCases,whereas its homologs in the closely related genera Xanthomonas and Stenotrophomonas belong to Group I,which contains most ODCases from Gram-positive bacteria,Gram-negative bacteria and ***,the Group I ODCase PXO_3614 from the Xanthomonas oryzae *** PXO99A strain complemented the Le0752 mutant in regulating HSAF-mediated antagonistic ***,these results highlight the important requirement of de novo pyrimidine biosynthetic enzymes for antibiotic HSAF production in ***,which lays an important foundation for improving HSAF production via metabolic flow design and for dissecting the regulatory functions of bacterial ODCases.

关键词： ODCase pyrimidine de novo synthesis secondary metabolite HSAF Lysobacter enzymogenes

在线全文

学校读者我要写书评

暂无评论

涤棉织物分散/活性染料同浆印花工艺

丝绸 2000年第8期37卷 10-11页

作者：钱国良金玮吴江绸缎炼染二厂江苏吴江215228

涤棉织物分散 /活性染料同浆印花的工艺重点 ,一是前处理中要进行丝光 ;二是印花原浆采用半乳化浆 ,染料选用耐碱型分散染料和稳定性好、对涤纶沾污小的活性染料 ,色浆中尿素用量控制在 7% ;三是水洗中加清洗剂处理。

关键词：涤绵织物分散染料活性染料同浆印花

在线全文

学校读者我要写书评

暂无评论

TD-SCDMA业务初探

电信技术 2005年第5期 10-12页

作者：钱国良普天信息研究院

TD-SCDMA技术是我国通信史上第一次由我国提出并被广泛认可的3G国际标准,也是世界上惟一的TDD模式的标准。作为我国自主研发并具有知识产权的3G国际标准之一,TD-SCDMA的发展备受各方的关注。自2004年产业链成熟、多厂家供货环境形成后,T... 详细信息

TD-SCDMA技术是我国通信史上第一次由我国提出并被广泛认可的3G国际标准,也是世界上惟一的TDD模式的标准。作为我国自主研发并具有知识产权的3G国际标准之一,TD-SCDMA的发展备受各方的关注。自2004年产业链成熟、多厂家供货环境形成后,TD-SCDMA独立组网在技术能力方面已经日渐完善,市场优势日益彰显,其优势突出表现在系统抗干扰和系统容量之间得到了很好地均衡、对混合业务的高效支持以及系统自身有良好的可持续发展和技术演进性等。本专题从TD-SCDMA采用的智能无线、软件无线电等技术,网络规划、业务探讨以及目前能否独立组网等方面进行全面的介绍,旨在让读者更好地了解TD-SCDMA技术。

关键词： CDMA业务 SCDMA系统移动数据业务市场需求 3G业务频谱利用率语音业务建网成本市场潜力业务实现用户群 TD 极大的 ITU 优势技术

变电所统计型电压表数据实时采集系统的开发和应用

在线全文

学校读者我要写书评

暂无评论

供用电 2005年第4期22卷 24-25页

作者：钱国良平湖市供电局浙江平湖314200

0 引言近年来,对电能质量的考核要求越来越高,电压合格率是衡量电能质量的重要指标,因此,如何提高电压合格率是各供电部门的重要工作之一.变电所实行无人值班以后,对母线电压的监视是通过调度自动化系统实现的,变电所的电压交流量由电... 详细信息

0 引言近年来,对电能质量的考核要求越来越高,电压合格率是衡量电能质量的重要指标,因此,如何提高电压合格率是各供电部门的重要工作之一.变电所实行无人值班以后,对母线电压的监视是通过调度自动化系统实现的,变电所的电压交流量由电压变送器转换为0～5 V的模拟量送入RTU,再远传到调度端,由调度端换算成实际电压.由于变送器的精度低、误差大,远方监视得到的电压与变电所母线实际电压有较大偏差,会误导监控人员的控制行为,造成电压合格率的下降.

关键词：电压合格率实时采集系统变电所表数据应用开发统计电能质量调度自动化

在线全文

学校读者我要写书评

暂无评论

胶状溶杆菌OH17菌株遗传操作系统的构建

南京农业大学学报 2017年第4期40卷 649-654页

作者：刘琳琳钱国良刘凤权南京农业大学植物保护学院江苏南京210095

[目的]建立胶状溶杆菌(Lysobacter gummosus)OH17菌株的遗传操作系统,通过基因重组方法来研究调控基因的功能及次级代谢产物的生物合成途径。[方法]分别将整合型突变质粒p JQ-pilR和用于同源重组的、包含参与抗菌物质生物合成的DNA片段... 详细信息

[目的]建立胶状溶杆菌(Lysobacter gummosus)OH17菌株的遗传操作系统,通过基因重组方法来研究调控基因的功能及次级代谢产物的生物合成途径。[方法]分别将整合型突变质粒p JQ-pilR和用于同源重组的、包含参与抗菌物质生物合成的DNA片段的质粒p SEVA321-pilR,通过电转化法各自导入*** OH17之中。[结果]通过电转化获得正确转化子,结合基因重组获得突变菌株,采用PCR验证和酶切验证重组质粒的导入和目标基因pilR的缺失,进一步对突变菌株的发酵产物进行了HPLC分析,结果表明突变株合成热稳定抗真菌因子(heat-stable antifungal factor,HSAF)能力明显下降,通过将pilR回补,其合成HSAF的能力得到一定程度恢复。[结论]成功构建了*** OH17遗传操作系统,包括基因定位缺失突变系统和基因功能互补系统,为以后对OH17及其他胶状溶杆菌进行代谢产物合成及其调控基因的功能研究奠定了遗传操作基础。

关键词：胶状溶杆菌遗传操作系统基因敲除基因互补

在线全文

学校读者我要写书评

暂无评论

基于C-NCAP的某车乘员约束系统的改进

辽宁工程技术大学学报（自然科学版） 2008年第3期27卷 417-419页

作者：张建荣韩忠浩钱国强辽宁工业大学汽车与交通工程学院辽宁锦州121001

为了提高某车在C-NCAP中的等级,在MADYMO中建立了某车正面100%碰撞的乘员约束系统的模型,经过调整验证了模型的正确性。按照C-NCAP的规定原车碰撞结果中驾驶员胸部压缩量偏高,分别采用了施加限力器和改变方向盘转向刚度的方法提高驾驶... 详细信息

为了提高某车在C-NCAP中的等级,在MADYMO中建立了某车正面100%碰撞的乘员约束系统的模型,经过调整验证了模型的正确性。按照C-NCAP的规定原车碰撞结果中驾驶员胸部压缩量偏高,分别采用了施加限力器和改变方向盘转向刚度的方法提高驾驶员的胸部压缩量,通过仿真分析改进方向盘转向刚度的方法达到了法规的要求,并通过分析提出了相关的其它改进方案。

关键词： C-NcAP 乘员约束系统