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Clinical Observation on Treatment of 60 Cases of Osteoarthritis of Knee Joint by Electroacupuncture

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Journal of Acupuncture and Tuina Science 2003年第4期1卷 38-40页

作者： DAI qi-ping qiu Min-lei SHAO ping HU dong WU Xue-fei Shanghai TCM Hospital 200071 China Shanghai TCM Hospital 200071 China Shanghai TCM Hospital 200071 China Shanghai TCM Hospital 200071 China

Purpose To observe therapeutic effect of electroacupuncture Neixiyan (Ex-LE 4) and Dubi (ST 35) in treating osteoarthritis of knee *** All the 120 cases were randomly divided into electroacupuncture and control groups, 60 cases in each group, and they were given electroacupuncture and Ritalin slowreleased tablet respectively, and pain, mobility and swelling degree of knee joint were observed before and after *** In treatment group, the average score increased by 18, while in control group, it increased by 12.33 (Pqi-ping (1966-), female, attending physicianTranslator: Wu Xue-fei

关键词： Electroacupuncture Point Ex-LE 4 (Neixiyan) Point ST 35 (Dubi) Osteoarthritis Knee Joint

Electrocatalytic cleavage of a lignin β-O-4 model compound and coupling with nitrogen-containing aromatics using Prussian blue analogue-derived nickel–cobalt spinel

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Journal of Energy Chemistry 2025年第1期100卷 628-636页

作者： Yi qi Xueying Chen Bowen Liu Xuliang Lin Xueqing qiu Yanlin qin Guangdong Provincial Key Laboratory of Plant Resources Biorefinery School of Chemical Engineering and Light Industry Guangdong University of Technology Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Guangdong Basic Research Center of Excellence for Ecological Security and Green Development Guangdong University of Technology

Electrochemical conversion of lignin for the production of high-value heterocyclic aromatic compounds has great *** demonstrate the targeted synthesis and cation modulation of NiCo2O4spinel nanoboxes,synthesized via cation exchange and calcination *** catalysts exhibit excellent efficacy in the electrocatalytic conversion of lignin model compounds,specifically 2-phenoxy-1-phenylethanol,into nitrogen-containing aromatics,achieving high conversion rates and *** catalysts were synthesized via a cation exchange and calcination oxidation process,using Prussian blue nanocubes as *** porous architecture and polymetallic composition of the NiCo2O4spinel demonstrated superior performance in electrocatalytic oxidative coupling,achieving a 99.2 wt%conversion rate of the 2-phenoxy-1-phenylethanol with selectivities of 37.5 wt%for quinoline derivatives and 31.5 wt%for *** innovations include the development of a sustainable one-pot synthesis method for quinoline derivatives,the elucidation of a multistage reaction pathway involving CAO bond cleavage,hydroxyaldol condensation,and CAN bond formation,and a deeper mechanistic understanding derived from DFT *** work establishes a new strategy for lignin valorization,offering a sustainable route to produce high-value nitrogen-containing aromatics from renewable biomass under mild conditions,without the need for additional reagents.

关键词： Spinel Biomass valorization Electrocatalysis Lignin oxidation Coupling

Method validation of lymphocyte subgroup phenotyping analysis in cynomolgus monkeys and Sprague-Dawley rats using flow cytometry

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Method validation of lymphocyte subgroup phenotyping analysi...

Method validation of lymphocyte subgroup phenotyping analysis in cynomolgus monkeys and Sprague-Dawley rats using flow cytometry

2013年（第三届）中国药物毒理学年会暨药物非临床安全性评价研究论坛

作者： SUN Jian-hua WANG Ying SUN Xiao-xun HUANG Gui-hua GAO Jing-li qi qiu-ping Center for Drug Safety Evaluation and Research Shanghai Institute of Materia MedicaChinese Academy of Sciences

OBJECTIVE To validate a flow-cytometry-based method for the measurement of lymphocyte subgroups in cynomolgus monkeys and Sprague Dawley rats,*** For cynomolgus monkeys,two panels of fluorescent antibodies,namely CD3-FITC/CD4-PerCP/CD8-PE and CD3-PE-Cy5.5/CD16-PE/CD20-FITC were used to analyze the proprotion of T cells including T helper cell(CD3 CD4) and T cytotoxic cells(CD3 CD8),NK cells(CD3 CD16) and B cells(CD3 CD20).For Sprague Dawley rats,other two panels of fluorescent antibodies,namely CD3 FITC/CD4-PE/CD8a-PerCP and CD3 FITC/CD45RA-PE-Cy5.5/CD161a-PE were used to analyze the proprotion of T cells,NK cells(CD3CD161) and B cells(CD3CD45RA).Blood samples from 10 monkeys and 10 rats were analyzed for intra-assay precision,sample stability and inter-subject ***,intra-animal precision was investigated in *** For cynomolgus monkeys,intra-assay CV and intra-animal CV for all parameters were within 15%.The positive binding signals of all isotype controls were less than 1%.Furthermore,monkey blood samples were stable for all parameters when kept at room temperature for 6 h or at 4℃for 1 *** after fixation were stable while stored in the refrigerator(+4℃) for 24 *** rats,intra-assay CVs for all parameters were within 15%.The positive binding signals of isotype controls of CD3/CD4 and CD3/CD8 were less than 1%.Furthermore,rat blood samples were stable for CD3/CD4 and CD3/CD8 while stored in the refrigerator(+4℃) for 24 ***,rat blood samples were unstable for CD3/161 a when kept at room temperature for 6h and unstable for CD3/45RA when kept at 4℃for 1 *** latter two cases,the positive binding signals of the isotype controls were around 2%.CONCLUSION All the parameters tested fulfilled the criteria of acceptance with a few minor exceptions related to stability data from rat blood samples. It is suitable for the measurement of lymphocyte subgroups in cynomolgus monkeys and Sprague Dawley rats.

关键词： lymphocyte phenotype flow cytometry

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Method validation of lymphocyte subgroup phenotyping analysi...

Method validation of lymphocyte subgroup phenotyping analysis in cynomolgus monkeys and Sprague-Dawley rats using flow cytometry

作者： SUN Jian-hua WANG Ying SUN Xiao-xun HUANG Gui-hua GAO Jing-li qi qiu-ping Center for Drug Safety Evaluation and Research Shanghai Institute of Materia MedicaChinese Academy of Sciences

OBJECTIVE To validate a flow-cytometry-based method for the measurement of lymphocyte subgroups in cynomolgus monkeys and Sprague Dawley rats,*** For cynomolgus monkeys,two panels of fluorescent antibodies,namely CD3-FITC/CD4-PerCP/CD8-PE and CD3-PE-Cy5.5/CD16-PE/CD20-FITC were used to analyze the proprotion of T cells including T helper cell(CD3~+CD4~+) and T cytotoxic cells(CD3~+CD8~+),NK cells(CD3~- CD16~+) and B cells(CD3~- CD20~+).For Sprague Dawley rats,other two panels of fluorescent antibodies,namely CD3~- FITC/CD4-PE/CD8a-PerCP and CD3-FITC/CD45RA-PE-Cy5.5/CD161a-PE were used to analyze the proprotion of T cells,NK cells(CD3~-CD161~+) and B cells(CD3~-CD45RA~+).Blood samples from 10 monkeys and 10 rats were analyzed for intra-assay precision,sample stability and inter-subject ***,intra-animal precision was investigated in *** For cynomolgus monkeys,intra-assay CV and intra-animal CV for all parameters were within 15%.The positive binding signals of all isotype controls were less than 1%.Furthermore,monkey blood samples were stable for all parameters when kept at room temperature for 6 h or at 4℃for 1 *** after fixation were stable while stored in the refrigerator(+4℃) for 24 *** rats,intra-assay CVs for all parameters were within 15%.The positive binding signals of isotype controls of CD3~+/CD4~+ and CD3~+/CD8~+ were less than 1%.Furthermore,rat blood samples were stable for CD3~+/CD4~+ and CD3~+/CD8~+ while stored in the refrigerator(+4℃) for 24 ***,rat blood samples were unstable for CD3/161a when kept at room temperature for 6h and unstable for CD3/45RA when kept at 4℃for 1 *** latter two cases,the positive binding signals of the isotype controls were around 2%.CONCLUSION All the parameters tested fulfilled the criteria of acceptance with a few minor exceptions related to stability data from rat blood samples. It is suitable for the measurement of lymphocyte subgroups in cynomolgus monkeys and S

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中国药理学与毒理学杂志 2013年第3期 513-513页

作者： SUN Jian-hua WANG Ying SUN Xiao-xun HUANG Gui-hua GAO Jing-li qi qiu-ping Center for Drug Safety Evaluation and Research Shanghai Institute of Materia MedicaChinese Academy of Sciences

OBJECTIVE To validate a flow-cytometry-based method for the measurement of lymphocyte subgroups in cynomolgus monkeys and Sprague Dawley rats,*** For cynomolgus monkeys,two panels of fluorescent antibodies,namely CD3-FITC /CD4-PerCP /CD8-PE and CD3-PE-Cy5.5 /CD16-PE /CD20-FITC were used to analyze the proprotion of T cells including T helper cell(CD3 + CD4 +) and T cytotoxic cells(CD3 + CD8 +),NK cells(CD3 CD16 +) and B cells(CD3-CD20 +).For Sprague Dawley rats,other two panels of fluorescent antibodies,namely CD3 FITC /CD4-PE /CD8a-PerCP and CD3-FITC /CD45RA-PE-Cy5.5 /CD161a-PE were used to analyze the proprotion of T cells,NK cells(CD3-CD161 +) and B cells(CD3-CD45RA +).Blood samples from 10 monkeys and 10 rats were analyzed for intra-assay precision,sample stability and inter-subject ***,intra-animal precision was investigated in *** For cynomolgus monkeys,intra-assay CV and intra-animal CV for all parameters were within 15%.The positive binding signals of all isotype controls were less than 1%.Furthermore,monkey blood samples were stable for all parameters when kept at room temperature for 6 h or at 4℃ for 1 *** after fixation were stable while stored in the refrigerator(+ 4℃) for 24 *** rats,intra-assay CVs for all parameters were within 15%.The positive binding signals of isotype controls of CD3 + /CD4 + and CD3 + /CD8 + were less than 1%.Furthermore,rat blood samples were stable for CD3 + /CD4 + and CD3 + /CD8 + while stored in the refrigerator(+ 4℃) for 24 ***,rat blood samples were unstable for CD3 /161a when kept at room temperature for 6h and unstable for CD3 /45RA when kept at 4℃ for 1 *** latter two cases,the positive binding signals of the isotype controls were around 2%.CONCLUSION All the parameters tested fulfilled the criteria of acceptance with a few minor exceptions related to stability data from rat blood *** is suitable for the measurement of lymphocyte subgroups in cynomolgus mo

关键词： lymphocyte phenotype flow cytometry

Amino Acids Catalyzed Direct Aldol Reactions in Aqueous Micelles

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Amino Acids Catalyzed Direct Aldol Reactions in Aqueous Mice...

Surface-confined persistent luminescence with amplified FRET efficiency for cell-cell communication monitoring

第三届全国有机化学学术会议

作者： PENG, Yi-Yuan WANG, qi DING, qiu-ping HE, Jia-qi CHENG, Jin-Pei Department of Chemistry, Nankai University, Tianjin 300071 Department of Chemistry, Jiangxi Normal University, Nanchang 330027

＜正＞ Since the discovery of its roles as a good small-organic-moleculecatalyst in intramolecular aldol reactions, proline has drawn considerableattention in synthetic chemistry due to its similarity to the type-Ialdolases. Recently, List and others have reported some new directasymmetric intermolecular reactions catalyzed by proline, includingaldol, Mannich, Michael, and other analogous reactions. Except for tworecent examples proline catalyzed al

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Science China Chemistry 2025年

作者： Ziyun Miao Wenjing Dai Yubin Jin Bing qi Jiayi Chen Lingling qiu Yurong Wei Jie Wang The Key Lab of Health Chemistry&Molecular Diagnosis of Suzhou College of ChemistryChemical Engineering&Materials ScienceSoochow University

Microbial communication monitoring is vital for understanding the mechanisms beneath gut microbiota equilibrium, infections,and biomanufacturing. Herein, we reported monitoring bacterial communication by surface-confined persistent ***, we constructed core-shell structured Zn1.4Ga1.2Ge0.4O4@Zn1.4Ga1.2Ge0.4O4:Cr（ZGGO@ZGGO:Cr） persistent luminescence nanoparticles（PLNPs） with amplified FRET efficiency for the detection of azoreductase（AzoR） that participates in bacterial communication. ZGGO@ZGGO:Cr nanoparticles were functionalized with an azo black hole quencher（ZGGO@ZGGO:Cr-BHQ-3）. Due to the proximity of surface-confined persistent luminescence with the surface-bound BHQ-3,ZGGO@ZGGO:Cr-BHQ-3 exhibited a FRET efficiency up to 1.6 times that of traditional ZGGO:Cr-BHQ-3. Further, the specific catalytic reduction of azo bonds in BHQ-3 by bacterial extracellular AzoR recovers the persistent luminescence of ZGGO@ZGGO:Cr. ZGGO@ZGGO:Cr-BHQ-3 displayed superior sensitivity and speed in detecting extracellular AzoR over ZGGO:Cr-BHQ-3, attributed to its surface-confined persistent luminescence. We found that the peak secretion of AzoR occurs during the exponential growth period of bacteria including S. aureus and E. coli based on the persistent luminescence recovery rate of ZGGO@ZGGO:Cr-BHQ-3. Furthermore, ZGGO@ZGGO:Cr-BHQ-3 was used to probe the communication between different bacterial species. R. palustris is a kind of lycopene-production bacteria. We found that the cohabitation of R. palustris and S. putrefaciens boosted both AzoR secretion and lycopene production. Moreover, the secretion and activity of AzoR positively correlated with lycopene production, suggesting that AzoR may participate in the communication between R. palustris and S. putrefaciens to promote lycopene production. This study offers a robust method to enhance PLNP-based biosensing sensitivity and provides a promising strategy for microbial communication monitoring.

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海斯特·白兰与简·爱之性格比较

南京农业大学学报（社会科学版） 2006年第4期6卷 88-92页

作者：齐秋萍黄汉平暨南大学文学院广东广州510632

19世纪中叶女作家夏洛蒂.勃朗特创作的简.爱,与同时代男作家纳丹尼尔.霍桑笔下的海斯特.白兰,作为维多利亚时代典范的叛逆女性形象为人们所熟悉。虽同为时代的反叛者,但相距近两百年的这两位女性形象,却具有迥然各异的性格特色。本文试... 详细信息

19世纪中叶女作家夏洛蒂.勃朗特创作的简.爱,与同时代男作家纳丹尼尔.霍桑笔下的海斯特.白兰,作为维多利亚时代典范的叛逆女性形象为人们所熟悉。虽同为时代的反叛者,但相距近两百年的这两位女性形象,却具有迥然各异的性格特色。本文试从宗教对主人公心理内化、与文本中其他人物关系以及文本中反复出现的特定意象隐喻三方面,从多侧面观照两位女主人公人物性格。

关键词：性格比较宗教内化人物关系特有意象

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储罐液位异常检测系统研究

软件 2018年第3期39卷 32-36页

作者：齐秋平文必龙吴婷东北石油大学计算机与信息技术学院黑龙江大庆163318

目前,对于时序数据的异常检测方面有很多较为成熟的模型,但并没有一套基于储罐液位数据进行分析、预测以及异常检测的系统。油田上不同开采工程的数据大都来自测量仪表、液位仪、传感器,具有时序性的特点,为充分时序数据的价值,提出了... 详细信息

目前,对于时序数据的异常检测方面有很多较为成熟的模型,但并没有一套基于储罐液位数据进行分析、预测以及异常检测的系统。油田上不同开采工程的数据大都来自测量仪表、液位仪、传感器,具有时序性的特点,为充分时序数据的价值,提出了一套基于油田储罐液位数据的异常检测系统,用于检测储罐液位数据。该系统以对油田储油罐液位的特征提取、异常检测、模式识别、预测分析建立框架,并可将其推广到油田钻井、测井、录井、井下作业等业务的异常检测。

关键词：时序数据储罐液位异常检测特征提取预测分析

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