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Electroacupuncture Attenuates Ischemic Brain Injury and Cellular Apoptosis via Mitochondrial Translocation of Cofilin

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Chinese Journal of Integrative Medicine 2021年第9期27卷 705-712页

作者： CHEN Bin LIN Wan-qing LI Zuan-fang ZHONG Xiao-yong WANG Jing YOU Xiao-fang ZHAO Hong-jia qi da-shi Department of Rehabilitation Medicine and National Clinical Research Base of Traditional Chinese Medicine the Affiliated People's Hospital of Fujian University of Traditional Chinese MedicineFuzhou(350004)China Academy of Integrative Medicine Fujian University of Traditional Chinese MedicineFuzhou(350108)China Shanghai Key Lab of Birth Defect Children's Hospital of Fudan UniversityShanghai(200032)China

Objective:To investigate the potential mechanisms of electroacupuncture(EA)to prevent ischemic ***:The method of middle cerebral artery occlusion(MCAO)was employed to establish a rat model of ischemic ***-eight Sprague-dawley rats were divided into the sham group,MCAO+EA control(EC)group,and MCAO+EA(EA)group according to a random number table(n=26 per group).EA was applied to the acupoints of Baihui(DU 20)and Shenting(DU 24)5 min and 6 h,respectively after the onset of MCAO for 30 *** in the sham and EC groups received only light isoflurane anesthesia for 30 min after *** neuroprotective effects of EA were evaluated by rota-rod test,neurological deficit scores and infarct ***,Nissl staining and immunostaining were performed to examine brain damage,rod formation,cellular apoptosis,and neuronal loss induced by *** activities of caspase-3,and expression levels of cofilin and p-cofilin in mitochondria and cytoplasm after ischemic injury were determined by Western ***:Compared with the EC group,EA significantly improved neuromotor function and cognitive ability after ischemic stroke(Pdation in the cortical penumbra area compared with the EC rats(Pdamage(infarct volume and neuropathy),cellular apoptosis and neuronal loss induced by ischemia were remarkably suppressed by EA in the cortical penumbra of rats(P<0.05 or P<0.01).Conclusion:EA treatment after ischemic stroke may attenuate ischemic brain injury and cellular apoptosis through the regulation of mitochondrial translocation of cofilin,a novel mechanism of EA therapy.

关键词： electroacupuncture cofilin apoptosis ischemic stroke mitochondrial translocation Chinese medicine

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Capillary Electrophoresis in the Presence of Fosfomycin

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Wuhan University Journal of Natural Sciences 2000年第2期5卷 210-214页

作者： XIE Min jie, FENG Yu qi , da shi lu College of Chemistry and Environmental Science, Wuhan University, Wuhan 430072, China Received date: 1999 09 29 Corresponding author Foundation item: Supported by the National Natural Science Foundation o Wuhan University College of Chemistry and Environmental Science Wuhan China (GRID:grid.49470.3e) (ISNI:***)

Fosfomycin, a sodium salt ofcis-(3-methyloxiranyl) phosphonic acid, was used as electrolyte in binary methanol-water media for capillary electrophoresis. The variety of electroosmotic flow with pH*, methanol concentration and ionic strength was investigated. The migration behavior of nine bases was examined under various conditions, and the separation of thymine, cytosine, 5-flurouracil, 4, 6-diamino-pyrimidine, purine was accomplished.

关键词： Key words fosfomycin electrophoresis electroomotic flow bases

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Flexible molecules dedicate to release strain of inverted inorganic perovskite solar cell

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Journal of Energy Chemistry 2025年第1期100卷 87-93页

作者： Hongrui Sun Sanlong Wang Pengyang Wang Yali Liu Shanshan qi Biao shi Ying Zhao Xiaodan Zhang Institute of Photoelectronic Thin Film Devices and Technology Renewable Energy Conversion and Storage Center State Key Laboratory of Photovoltaic Materials and CellsNankai University Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin Haihe Laboratory of Sustainable Chemical Transformations Engineering Research Center of Thin Film Photoelectronic Technology of Ministry of Education Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)

The tensile strain in inorganic perovskite films induced by thermal annealing is one of the primary factors contributing to the inefficiency and instability of inorganic perovskite solar cells （IPSCs）,which reduces the defect formation ***,a flexible molecule 5-maleimidovaleric acid （5-MVA） was introduced as a strain buffer to release the residual strain of *** anhydride and carboxyl groups in 5-MVA interact strongly with the uncoordinated Pb2+through Lewis acid-base reaction,thus tightly“pull”the perovskite *** in-between soft carbon chain increased the structural flexibility of CsPbI2.85Br0.15perovskite materials,which effectively relieved the intrinsic internal strain of CsPbI2.85Br0.15,resisted the corrosion of external strain,and also reduced the formation of defects such as VIand *** addition,the introduction of 5-MVA improved crystal quality,passivated residual defects,and narrowed energy level ***,power conversion efficiency （PCE） of NiOxbased inverted IPSCs increased from 19.25% to 20.82% with the open-circuit voltage enhanced from 1.164 V to 1.230 *** release of strain also improved the stability of CsPbI2.85Br0.15perovskite films and devices.

关键词： Perovskite solar cells

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A matrix metalloproteinase-responsive hydrogel system controls angiogenic peptide release for repair of cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2025年第2期20卷 503-517页

作者： qi Liu Jianye Xie Runxue Zhou Jin Deng Weihong Nie Shuwei Sun Haiping Wang Chunying shi Department of Human Anatomy Histology and EmbryologySchool of Basic MedicineQingdao UniversityQingdaoShandong ProvinceChina Department of Neurology The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina Department of General Practice The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina

Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic ***,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in ***,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic *** metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ***,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel ***-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in *** results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor *** findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.

关键词： angiogenesis biomaterial blood-brain barrier cerebral ischemia/reperfusion injury control release drug delivery inflammation QK peptides matrix metalloproteinase-2 neuroprotection self-assembling nanofiber hydrogel

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Single-cell technologies: current and near future

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Science China Life Sciences 2025年第01期 1-4页

作者： Chenfei Wang qi Liu Xiaohui Fan Tieliu shi Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration Ministry of EducationOrthopedics DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Key Laboratory of Advanced Theory and Application in Statistics and data Science (MOE) School of StatisticsEast China Normal University

Single-cell technologies enable the indepth exploration of multiple biological hierarchies at the scale of individual cells,which have deepened our knowledge of cellular diversity, tissue organization, and overall organism function(Sun et al.,2024). In 2009, single-cell RNA sequencing(sc RNA-seq) was first developed as a powerful tool to dissect gene expression and uncover transcriptional *** the rapid advancement of technology, single-cell approaches have expanded to encompass other omics, such as genomics, epigenomics, proteomics,and metabolomics.

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核质因素对T型杂种小麦种子质量及其稳定性的影响

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西南农业学报 1999年第1期12卷 26-31页

作者：刘仲齐饶世达蒲宗君四川省农科院作物所

１９９３～１９９６，以Ｔ型不育系、保持系和恢复系为材料，对影响Ｔ型杂种小麦种子质量及其稳定性的核质因素进行了研究。结果表明，多数不育系种子的粒重和发芽率仅分别相当于保持系的９０％和５０％左右，年份间的变异较大；有些不... 详细信息

１９９３～１９９６，以Ｔ型不育系、保持系和恢复系为材料，对影响Ｔ型杂种小麦种子质量及其稳定性的核质因素进行了研究。结果表明，多数不育系种子的粒重和发芽率仅分别相当于保持系的９０％和５０％左右，年份间的变异较大；有些不育系种子的发芽率在年份间的变异系数高达２３．７％。只有极少数不育系的种子质量与保持系基本相同，且稳定性较好。不育系种子的皱缩程度和发芽率在基因型间存在极显著的差异。不育系和恢复系杂交产生的杂交种子，其粒重主要取决于不育系，发芽率则与双亲都有关系，当不育系种子的的发芽率在７０％以上时，杂交种的发芽率与双亲发芽率的乘积呈高度正相关，ｒ＝０．８５０＊＊。正反交试验表明，恢复基因可以有效地消除Ｔ型胞质对种子质量的负效应。普通小麦中的非恢复基因也能有效地抑制Ｔ质对种子发育的不良效应，但这类基因的频率很低，其遗传规律尚需进一步的研究。

关键词：杂种小麦种子质量稳定性 T型细胞质小麦

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不同地域乌拉尔甘草基因组的FISH分析与染色体识别

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西北植物学报 2010年第2期30卷 262-268页

作者：徐汉杰汤佳立戚大石李宗芸徐州师范大学生命科学学院江苏省药用植物生物技术重点实验室江苏徐州221116

在核型分析与染色体识别基础上,分别以番茄45S和5S rDNA为探针,对3种不同地域的乌拉尔甘草进行FISH分析。结果表明:内蒙古鄂托克前旗的乌拉尔甘草核型公式为2n=2x=16=6m+10sm(2SAT),新疆阿勒泰地区的乌拉尔甘草核型公式为2n=2x=16=4m+12... 详细信息

在核型分析与染色体识别基础上,分别以番茄45S和5S rDNA为探针,对3种不同地域的乌拉尔甘草进行FISH分析。结果表明:内蒙古鄂托克前旗的乌拉尔甘草核型公式为2n=2x=16=6m+10sm(2SAT),新疆阿勒泰地区的乌拉尔甘草核型公式为2n=2x=16=4m+12sm(2SAT),内蒙古喀喇沁旗乌拉尔甘草核型公式为2n=2x=16=4m+12sm(2SAT);其第8染色体均带有随体。3种乌拉尔甘草基因组内均有1对5S rDNA和1对45SrDNA杂交位点。核型分析显示,5S rDNA杂交位点均位于第2染色体的短臂部位,45S rDNA杂交位点均位于第8染色体的次缢痕和随体部位。45S与5S rDNA在3种乌拉尔甘草中期分裂相上的位点数和分布情况高度一致,表明来自3种不同地域的乌拉尔甘草在染色体结构水平上没有较大的分化。

关键词：乌拉尔甘草 rDNA 荧光原位杂交染色体识别

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不同染色技术和双色荧光原位杂交技术对宽翅菘蓝(Isatis violascens Bunge)的细胞遗传学分析

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现代生物医学进展 2011年第3期11卷 485-488页

作者：戚大石汤佳立徐州医学院江苏徐州221121 中国药科大学生命科学与技术学院江苏南京210009

为了解十字花科菘蓝属植物宽翅菘蓝(宽翅菘蓝,Isatis violascens Bunge)的染色体结构,本文利用45SrDNA和5SrDNA双色荧光原位杂交、银染技术和CMA3对宽翅菘蓝进行分子细胞遗传学研究。45SrDNA和5SrDNA荧光原位杂交结果显示宽翅菘蓝染色... 详细信息

为了解十字花科菘蓝属植物宽翅菘蓝(宽翅菘蓝,Isatis violascens Bunge)的染色体结构,本文利用45SrDNA和5SrDNA双色荧光原位杂交、银染技术和CMA3对宽翅菘蓝进行分子细胞遗传学研究。45SrDNA和5SrDNA荧光原位杂交结果显示宽翅菘蓝染色体上有1对45SrDNA信号和1对5SrDNA信号;银染结果显示其中期染色体有1对银染点;CMA3染色结果发现宽翅菘蓝中期染色体存在1对CMA3信号。

关键词：宽翅菘蓝银染 45SrDNA 双色荧光原位杂交 CMA3染色

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高纯度少突胶质前体细胞改良培养方法的建立

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上海交通大学学报（医学版） 2010年第11期30卷 1444-1447页

作者：王兴启刘轩杨丽华戚大石刘静姚瑞芹徐州医学院神经生物学教研室徐州221002

目的对传统培养方法进行改良,以获得更高纯度的少突胶质前体细胞(OPCs)。方法新生1～2d Sprague-dawley(SD)大鼠的大脑皮质混合胶质细胞原代培养8d后,采用恒温摇床振荡分离法、差速贴壁法和胰酶消化法结合条件限定培养基纯化培养细胞,... 详细信息

目的对传统培养方法进行改良,以获得更高纯度的少突胶质前体细胞(OPCs)。方法新生1～2d Sprague-dawley(SD)大鼠的大脑皮质混合胶质细胞原代培养8d后,采用恒温摇床振荡分离法、差速贴壁法和胰酶消化法结合条件限定培养基纯化培养细胞,台盼蓝染色光学显微镜观察细胞形态;纯化培养2d后进行A2B5和daPI免疫荧光染色鉴定和细胞纯度分析。结果光学显微镜观察发现纯化培养细胞的胞膜完整,细胞无损伤;免疫荧光染色后细胞纯度分析显示,A2B5染色阳性细胞占daPI染核细胞的百分比为98.14%。结论通过对传统的混合胶质细胞培养及振荡分离和纯化方法的改进,能够培养出高纯度的OPCs。

关键词：少突胶质前体细胞细胞培养纯化鉴定大鼠

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High-selectivity electroreduction of low-concentration CO2 with large concentration fluctuation

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Science China Chemistry 2025年

作者： Mengyu qi Yanbin Ma Chao Zhang Bingwei Li Xueqing Yang Zhaolin shi Simeng Liu Changhua An Jiqing Jiao Tongbu Lu MOE International Joint Laboratory of Materials Microstructure Analysis and Testing CenterInstitute for New Energy Materials and Low Carbon TechnologiesSchool of Materials Science and EngineeringSchool of Chemistry and Chemical EngineeringTianjin University of Technology Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University

Electrochemical CO2reduction reaction（CO2RR） has been regarded as one of the most promising solutions to achieving “zero carbon emission”. In most of the CO2RR-related studies, high-purity CO2has been employed as the feed gas; however, in practice, CO2is generally emitted in low concentrations, so it is of great significance to realize high-selectivity electroreduction of low-concentration CO2with large concentration fluctuation. In this work, we constructed a dual-active-site catalyst and successfully achieved CO2local enrichment and conversion for low-concentration CO2. Operando experiments reveal that the catalyst has one type of site for activating CO2and one type of site for binding the reaction intermediates. The dual-active-site catalyst displays a selectivity for formic acid consistently above 97% over a broad potential window（from-0.9 to-1.6 V ***）. Even when fed with a low-concentration CO2stream（volume ratio from 50% down to 10%）, the dual-active-site catalyst could display high activity and selectivity（>91%）. In particular, the selectivity is still above 85% when the CO2volume ratio is as low as 5%. This work offers a feasible route for converting low-concentration CO2via a synergistic effect for dual-active-site catalysts.

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