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An intravenous clarithromycin lipid emulsion with a high drug loading, H-bonding and a hydrogen-bonded ion pair complex exhibiting excellent antibacterial activity

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Asian Journal of Pharmaceutical Sciences 2016年第5期11卷 618-630页

作者： Haoyu Gong Sicong Geng Qi Zheng puxiu wang Lifeng Luo Xiuzhi wang Yan Zhang Yu Zhang Haibing He Xing Tang Shenyang Pharmaceutical University

The aim of this study was to develop an intravenous clarithromycin lipid emulsion(CLE)with good stability and excellent antibacterial activity. The CLE was prepared by the thinfilm dispersed homogenization method. The interaction between clarithromycin(CLA) and cholesteryl hemisuccinate(CHEMS) was confirmed by DSC, FT-IR and^1H NMR analysis. The interfacial drug loading, thermal sterilization, freeze–thaw stability, and in vitro and in vivo antibacterial activity were investigated systematically. DSC, FT-IR and^1H NMR spectra showed that CHEMS(CLA: CHEMS, M ratio 1:2) could interact with CLA through H-bonding and a hydrogen-bonded ion pair. The CHEMS was found necessary to maintain the stability of *** showed that almost 88% CLA could be loaded into the interfacial *** optimized CLE formulation could withstand autoclaving at 121 °C for 10 min and remain stable after three freeze–thaw cycles. The in vitro susceptibility test revealed that the CLA–CHEMS ion-pair and CLE have similar activity to the parent drug against many different bacterial strains. The in vivo antibacterial activity showed that the ED50 of intravenous CLE was markedly lower than that of CLA solution administrated orally. CLE exhibited pronounced antibacterial activity and might be a candidate for a new nanocarrier for CLA with potential advantages over the current commercial formulation.

关键词： Clarithromycin Cholesteryl hemisuccinate H-bonding and hydrogen-bonded ion pair complex Antibacterial activity Thin-film dispersed homogenization

电影文化地理的时空建构意义——空间美学视野下的国产电影综述

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电影评介 2020年第14期 1-4页

作者：王璞琇中国传媒大学艺术研究院

一、概念辨析与综述视角选择依据文化地理是时空相交错而产生的意义在客体世界的投射和体现。在《宗教生活的基本形式》中,法国社会学家涂尔干指出,空间和时间是社会构造物。研究者姑且不论时空在先验世界中是否以实体方式存在,只谈在... 详细信息

一、概念辨析与综述视角选择依据文化地理是时空相交错而产生的意义在客体世界的投射和体现。在《宗教生活的基本形式》中,法国社会学家涂尔干指出,空间和时间是社会构造物。研究者姑且不论时空在先验世界中是否以实体方式存在,只谈在文艺作品中,时空带有非常明显的建构色彩;它一方面是当下流行的时空观在艺术品中的体现,另一方面又协助当代社会中的人建构和接受新的时空观。

关键词：客体世界时空观姑且不论时空建构先验世界国产电影涂尔干文艺作品

载人航天器座舱大气控制系统优化设计方法的探讨

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航天医学与医学工程 1988年第2期 85-92页

作者：王普秀航天医学工程研究所北京

本文把最优化原理用于载人航天器座舱大气控制系统设计,以系统的重量、体积、可靠性、成本和技术复杂程度为目标,建立了系统及其部组件的目标函数和有关性能计算公式,并用BASIC 语言编制了计算机设计程序,在微型计算机上进行了实例计算... 详细信息

本文把最优化原理用于载人航天器座舱大气控制系统设计,以系统的重量、体积、可靠性、成本和技术复杂程度为目标,建立了系统及其部组件的目标函数和有关性能计算公式,并用BASIC 语言编制了计算机设计程序,在微型计算机上进行了实例计算,证明本优化设计方法能够用于载人航天座舱大气控制系统总体设计。

关键词：生保系统航天器座舱座舱大气净化系统最优化设计控制系统

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梅兰芳的艺术合作网络探微

内蒙古师范大学学报（哲学社会科学版） 2020年第2期49卷 118-121页

作者：王璞琇中国传媒大学艺术研究院北京100024

国内外对于梅兰芳的学术研究研究颇多,大凡都是以梅兰芳在舞台上的具体表演为研究核心,而本文侧重于从艺术社会学方面的前沿理论"艺术界"的角度来研究梅兰芳所处的艺术关系网络的合作模式,意在能够在这一领域内,对戏曲艺术发展的规律和... 详细信息

国内外对于梅兰芳的学术研究研究颇多,大凡都是以梅兰芳在舞台上的具体表演为研究核心,而本文侧重于从艺术社会学方面的前沿理论"艺术界"的角度来研究梅兰芳所处的艺术关系网络的合作模式,意在能够在这一领域内,对戏曲艺术发展的规律和方向做社会学的把握,并期望对艺术家和艺术界的良性发展起到一定的启迪和辅助作用。

关键词：梅兰芳艺术界合作惯例齐如山

miR-138-5p靶向调控RAB22A表达抑制胶质瘤细胞的增殖、迁移和侵袭

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现代肿瘤医学 2023年第3期31卷 401-406页

作者：李旭王璞修刘国力中国医科大学附属第一医院药学部辽宁沈阳110001

目的:探讨miR-138-5p对胶质瘤细胞增殖、迁移与侵袭的影响以及其作用机制。方法:U251细胞分为miR-NC组(对照组)、miR-mimics组、miR-inhibitor组以及miR-mimics+RAB22A-pcDNA3.1组。qRT-PCR检测miR-138-5p在胶质瘤细胞中的表达量;CCK8实验检测U251细胞的增殖能力;Transwell实验检测U251细胞的迁移和侵袭能力;Targetscan数据库预测miR-138-5p的下游靶基因;运用双荧光素酶报告基因实验验证细胞周期蛋白RAB22A是miR-138-5p的靶基因;RNA结合蛋白免疫沉淀实验进一步验证miR-138-5p与RAB22A之间的相互作用;运用蛋白免疫印迹实验检测组织和细胞中RAB22A的蛋白表达水平。结果:与正常人星形胶质细胞相比较,miR-138-5p在胶质瘤细胞中表达量降低(P<0.05);CCK8和Transwell实验结果表明,在U251细胞中,过表达miR-138-5p能显著抑制细胞的增殖、迁移和侵袭能力(P<0.05);运用Targetscan数据库预测miR-138-5p下游靶基因为RAB22A,miR-138-5p作用于RAB22A的3'UTR区域,双荧光素酶报告基因实验与蛋白免疫沉淀实验结果证实miR-138-5p与RAB22A之间的相互作用;RAB22A在胶质瘤细胞中明显高表达(P<0.05),在U251细胞中过表达miR-138-5p明显抑制RAB22A的表达(P<0.05)。结论:miR-138-5p在胶质瘤细胞中表达降低,miR-138-5p通过下调RAB22A表达而抑制细胞增殖、迁移和侵袭。

关键词： miR-138-5p RAB22A 胶质瘤增殖迁移侵袭

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霜层冰晶体的生长模拟及其热导率的计算

化工学报 2009年第5期60卷 1111-1115页

作者：蔡亮王荣汉侯普秀张小松东南大学能源与环境学院江苏南京210096 华南理工大学机械与汽车工程学院广东广州510641

目前对霜层热导率的研究中,大多把霜层看作均匀的结构,而实际上霜层的热导率不仅取决于密度,同时也受霜层结构的影响。本文分析了实验观察到的霜晶体结构,提出了不同阶段的霜层生长模型,并采用计算机方法模拟这个过程。以模拟所得的霜... 详细信息

目前对霜层热导率的研究中,大多把霜层看作均匀的结构,而实际上霜层的热导率不仅取决于密度,同时也受霜层结构的影响。本文分析了实验观察到的霜晶体结构,提出了不同阶段的霜层生长模型,并采用计算机方法模拟这个过程。以模拟所得的霜晶体结构为基础,对每个节点列出了能量平衡方程,进而求得了霜层的热导率。结果表明,这种模拟方法较准确地反映了霜层中晶体的生长过程,计算所得的热导率也反映出霜层不同生长阶段的特点。

关键词：霜生长模型热导率结霜冰晶体计算机模拟

Effect of forsythoside A on the transcriptional profile of bovine mammary epithelial cells challenged with lipoteichoic acid

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Effect of forsythoside A on the transcriptional profile of b...

Analysis of differentially expressed microRNAs in bovine mammary epithelial cells treated with lipoteichoic acid

中国畜牧兽医学会兽医产科学分会第十六次学术研讨会

作者： Shen puxiu Yu Jingcheng Long Xiaochuan Huang Xiankai Tong Chao wang Xinzhuang

Objective:To screen the differentially expressed genes and related mechanisms of Staphylococcus aureus lipoteichoic acid（LTA） stimulation of bovine mammary epithelial cells and forsythiin A（FTA） ***:Bovine mammary epithelial cells（BMECs） were stimulated with LTA in vitro,and then treated with ***-Seq technique was used to detect differentially expressed *** was verified by *** analysis of differentially expressed genes was performed using gene Ontology（GO） and Kyoto Encyclopedia of Genes and Genomes（KEGG）.Result:The results showed that cell division cycle 20 B（CDC20 B）,endothelial cell surface expressed chemotaxis and apoptosis regulator（ECSCR）,complement factor H-related 5（CFHR5）and phospholipase A2 group ⅣA（PLA2G4A） were down-regulated after FTA *** contrast,Kruppel-like factor 15（KLF15） and Metallothionein 1 E（MT1 E） were *** DEGs are involved in processes such as apoptosis,inflammation and development of ***-qPCR was used to verify the RNA-Seq *** results showed a consistent trend of RNA-Seq expression,confirming our speculated mRNA expression ***:Kruppel-like factors（KLFs） are a family of transcription factors whose different domains enable them to play different roles in ***15 has been implicated in many cellular processes（such as apoptosis）,as well as in many *** A2（PLA2）,secreted and released by activated monocytes,macrophages and *** is involved in the release of inflammatory mediators and other biological ***2 G4 A is one of *** this study,upregulation of KLF15 expression among these six DEGs can inhibit the activation of P53,AKT/mTOR and P38/MAPK/ERK1/2 signalling pathways,thus reducing *** of PLA2 G4 A expression can inhibit PLA2G4A/AA and reduce the expression of inflammatory *** discovery of the role of DEGs verified the protective effect of FTA on BMECs after injury;however,the more speci

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Analysis of differentially expressed microRNAs in bovine mam...

Analysis of miRNA expression changes in bovine endometrial stromal cells treated with lipopolysaccharide

中国畜牧兽医学会兽医产科学分会第十六次学术研讨会

作者： Shen puxiu Yu Jingcheng Yan Chenbo Yang Dexin Tong Chao wang Xinzhuang College of Veterinary Medicine Henan Agricultural University

Objective:The purpose of this study was to screen the target genes and related molecular mechanisms involved in differential expression of microRNAs(miRNAs) in bovine mammary epithelial cells(BMECs) induced by Staphylococcus aureus lipoteichoic acid(LTA),so as to provide new targets for the diagnosis and treatment of ***:BMECs were treated with LTA,and RNA-Seq was used to screen differentially expressed miRNAs and their target *** verification was performed by ***:Compared to the control group,43 miRNAs were significantly up-regulated and eight miRNAs were significantly ***,724 genes were significantly upregulated and 13 genes were significantly down-regulated in LTA group relative to the control ***-miR-196 a,bta-miR-2285 aj-5 p,bta-miR-143,bta-miR-2433,bta-miR-2284 f and bta-miR-2368-3 p were selected from 51 differentially expressed miRNAs and are discussed in this *** gene prediction revealed that the target genes of these six miRNAs were all differentially expressed,including MT1 E,SPDYA,FGL1,TLR2,PAPOLG,ZDHHC17 and ***,the Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed that the target genes with differentially expressed miRNAs were enriched in mitogen-activated protein kinase(MAPK) signalling pathway,rheumatoid arthritis and ***:Bovine mastitis can be divided into clinical and subclinical *** detection and treatment have little effect on reproductive performance and can prevent transmission to other *** identification of specific and sensitive biomarkers is important for the early detection and treatment of *** are key regulatory factors in cell signaling pathways and various pathological *** conclusion,the results of this study suggest that bta-miR-143 may promote the expression of downstream pathways,such as NF-κB and MAPK,by promoting TLR2 receptor expression after LTA ***-miR-1

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Analysis of miRNA expression changes in bovine endometrial s...