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Laparoscopic radical prostatectomy： oncological and functional results of 126 patients with a minimum 3-year follow-up at a single Chinese institute

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Asian Journal of Andrology 2009年第5期11卷 548-556,I0001,I0002页

作者： Xin Gao jian-Hua zhou liao-Yuan li jian-guang Qiu Xiao-yong pu Department of Urology The Third Affiliated Hospital Sun Yat-Sen University Guangzhou 510630 China

In this study we evaluate the oncological and functional results of the largest cohort of patients in China treated by laparoscopic radical prostatectomy （LRP） and with at least 3 years of follow-up. 126 inconsecutive patients （range 56-78 years, median 62.5） who had an LRP were retrospectively analyzed. The mean prostate specific antigen level and Gleason score was 13.4 ng mL^-1 and 6.4, respectively. Twenty-seven patients had unilateral or bilateral nerve preservation and 29 had pelvic lymphadenectomy. Multivariate analysis was used to adjust for differences in clinical and pathological features when comparing the risk for biochemical progression-free survival （bPFS）. Urinary continence was assessed by incontinence questionnaire and erectile function by the Sexual Health Inventory for Men score. The mean operative duration was 250 min and blood loss 354 mL. Five patients received blood transfusion and nine had complications, including rectal injury （two）, ureteral injury （one）, active bleeding （one）, bladder neck stenosis （two）, paralytic ileus （one）, subcutaneous hematoma （one） and port-site hernia （one）. The overall positive surgical margin rate was 20.6% and correlated with pathological stage and Gleason score respectively （P = 0.03, P 〈 0.001 respectively）. All patients had 〉 3 years of follow-up （range 3-6.75 years, mean 4.6, median 4.75）. At 3 years of follow-up, the overall survival rate was 100% and the bPFS was 81.0% in all patients; 124 patients （98.4%） were continent; 22 of 27 patients （81.5%） who underwent nerve preservation retained erectile function. Our series confirms that LRP is an effective, safe and precise technique at Chinese institution.

关键词： complication follow-up laparoscopic surgery prostate cancer prostatectomy survival

Roles of Rho/Rock Signaling Pathway in Silica-induced Epithelial-mesenchymal Transition in Human Bronchial Epithelial Cells

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Biomedical and Environmental Sciences 2013年第7期26卷 571-576页

作者： HU yong Bin li Xiang liANG guan Nan DENG Zheng Hao jianG Hai Ying zhou jian Hua Department of Pathology Xiangya Basic Medical School Central South University

Objective To investigate the roles of Rho/Rock signaling pathway in silica-induced Epithelial-mesenchymal transition （EMT） in human bronchial epithelial cells （BEC） in vitro. Methods Human BEC were incubated with silica with various concentrations for indicated times. Cell viability was assayed by MTT test. Morphologic Changes were observed by microscope. Mesenchymal marker a-smooth muscle actin （a-SMA）, vimentin （Vim）, and epithelial marker E-cadherin （E-cad） were analyzed by Western Blot. The pull-down assay was used to measure Rho activity. In the prevention experiments, the specific inhibitor for Rho effector ROCK （Y27632） was used to inhibit the activity of Rho. Results Human BEC stimulated with silica were converted from a ＂cobblestone＂ epithelial structure into an elongated fibroblast-like shape structure. Incubation of human BEC with silica induced de novo expression of a-SMA and Vim, and loss of E-cad. Also, silica treatment resulted in Rho activation in human BEC. Y27632 up-regulated the E-cad expression but attenuated a-SMA and Vim expression in silica-stimulated cells. Conclusion The activation of Rho/ROCK signaling pathways is most likely involved in Silica-induced EMT in human bronchial epithelial cells.

关键词： Silica Bronchial epithelial cell Epithelial-to-mesenchymal transition Rho

Therapeutic effects of adipose-derived stem cells-based microtissues on erectile dysfunction in streptozotocin-induced diabetic rats

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Asian Journal of Andrology 2017年第1期19卷 91-97页

作者： Feng zhou Hua Xin yong-De Xu Hong-En Lei Bi-Cheng Yang Rui-li guan Meng li jian-Quan Hou Zhong-Cheng Xin Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing 100034 China Department of Urology First Affiliated Hospital of Soochow University Soochow University Suzhou 215006 China Department of Ophthalmology Beijing Chao-Yang Hospital Capital Medical University Beijing 100043 China Department of Urology General Hospital of Ningxia Medical University Ningxia Medical University Ningxia 750021 China

This study aimed to explore the therapeutic effects of adipose-derived stem cells （ADSCs）-based microtissues （MTs） on erectile dysfunction （ED） in streptozotocin （STZ）-induced diabetic rats. Fifty-six 8-week-old Sprague-Dawley rats received intraperitoneal injection of STZ （60 mg kg-1）, and 8 weeks later, the determined diabetic rats randomly received intracavernous （IC） injection of phosphate buffer solution （PBS）, ADSCs, or MTs. Another eight normal rats equally got IC injection of PBS. MTs were generated with a hanging drop method, and the injected cells were tracked in ADSC- and MT-injected rats. Four weeks after the treatments, intracavernous pressure （ICP）, histopathological changes in corpus cavernosum （CC）, and functional proteins were measured. Rat cytokine antibody array was used to detect ADSCs or MTs lysate. The results showed that MTs expressed vascular endothelial growth factor （VEGF）, nerve growth factor （NGF）, and tumor necrosis factor-stimulated gene-6 （TSG-6）. MTs injection had a higher retention than ADSCs injection and MTs treatment improved ICP, neuronal nitric oxide synthase （nNOS） expression, smooth muscle, and endothelial contents in diabetic rats, ameliorated local inflammation in CC better. Thus, our findings demonstrate that IC injection of MTs improves erectile function and histopathological changes in STZ-induced diabetic rats and appears to be more promising than traditional ADSCs. The underlying mechanisms involve increased cell retention accompanied with neuroprotection and anti-inflammatory behaviors of the paracrine factors.

关键词： adipose-derived stem cells diabetes erectile dysfunction microtissues paracrine factors

Genetic dissection of crown root traits and their relationships with aboveground agronomic traits in maize

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Journal of Integrative Agriculture 2023年第11期22卷 3394-3407页

作者： SHA Xiao-qian guan Hong-hui zhou Yu-qian SU Er-hu GUO jian li yong-xiang ZHANG Deng-feng liU Xu-yang HE guan-hua li Yu WANG Tian-yu ZOU Hua-wen li Chun-hui College of Agriculture Yangtze UniversityJingzhou 434000P.R.China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Crops Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China Institute of Maize Research Inner Mongolia Academy of Agricultural&Animal Husbandry SciencesHohhot 010031P.R.China

The crown root system is the most important root component in maize at both the vegetative and reproductive stages. However, the genetic basis of maize crown root traits(CRT) is still unclear, and the relationship between CRT and aboveground agronomic traits in maize is poorly understood. In this study, an association panel including 531 elite maize inbred lines was planted to phenotype the CRT and aboveground agronomic traits in different field environments. We found that root traits were significantly and positively correlated with most aboveground agronomic traits, including flowering time, plant architecture and grain yield. Using a genome-wide association study(GWAS)coupled with resequencing, a total of 115 associated loci and 22 high-confidence candidate genes were identified for CRT. Approximately one-third of the genetic variation in crown root was co-located with 46 QTLs derived from flowering and plant architecture. Furthermore, 103 (89.6%) of 115 crown root loci were located within known domestication-and/or improvement-selective sweeps, suggesting that crown roots might experience indirect selection in maize during domestication and improvement. Furthermore, the expression of Zm00001d036901, a high-confidence candidate gene, may contribute to the phenotypic variation in maize crown roots, and Zm00001d036901 was selected during the domestication and improvement of maize. This study promotes our understanding of the genetic basis of root architecture and provides resources for genomics-enabled improvements in maize root architecture.

关键词： maize root aboveground agronomic traits GWAS candidate genes

Sepsis-induced immunosuppression:mechanisms,diagnosis and current treatment options

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Military Medical Research 2023年第2期10卷 242-261页

作者： Di liu Si-Yuan Huang jian-Hui Sun Hua-Cai Zhang Qing-li Cai Chu Gao li li Ju Cao Fang Xu yong zhou Cha-Xiang guan Sheng-Wei Jin Jin Deng Xiang-Ming Fang jian-Xin jiang ling Zeng Department of Trauma Medical Center Daping HospitalState Key Laboratory of TraumaBurns and Combined InjuryArmy Medical UniversityChongqing 400042China Department of Respiratory Disease Daping HospitalArmy Medical UniversityChongqing 400042China Department of Laboratory Medicine the First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Critical Care Medicine the First Affiliated Hospital of Chongqing Medical University400016 ChongqingChina Department of Physiology School of Basic Medicine ScienceCentral South UniversityChangsha 410078China Department of Anesthesia and Critical Care the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityZhejiang 325027 WenzhouChina Department of Emergency the Affiliated Hospital of Guizhou Medical UniversityGuizhou Medical University550001 GuiyangChina Department of Anesthesiology the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310052China.

Sepsis is a common complication of combat injuries and trauma,and is defined as a life-threatening organ dysfunction caused by a dysregulated host response to *** is also one of the significant causes of death and increased health care costs in modern intensive care *** use of antibiotics,fluid resuscitation,and organ support therapy have limited prognostic impact in patients with *** its pathophysiology remains elusive,immunosuppression is now recognized as one of the major causes of septic ***-induced immunosuppression is resulted from disruption of immune *** is characterized by the release of antiinflammatory cytokines,abnormal death of immune effector cells,hyperproliferation of immune suppressor cells,and expression of immune *** targeting immunosuppression,especially with immune checkpoint inhibitors,preclinical studies have demonstrated the reversal of immunocyte dysfunctions and established host ***,we comprehensively discuss recent findings on the mechanisms,regulation and biomarkers of sepsis-induced immunosuppression and highlight their implications for developing effective strategies to treat patients with septic shock.

关键词： Sepsis Immunosuppression Immune monitoring Immunomodulatory therapy

Artificial intelligence empowers the second-observer strategy for colonoscopy:a randomized clinical trial

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Gastroenterology Report 2023年第1期11卷 216-223页

作者： pu Wang Xiao-Gang liu Min Kang Xue Peng Mei-ling Shu guan-Yu zhou Pei-Xi liu Fei Xiong Ming-Ming Deng Hong-Fen Xia jian-Jun li Xiao-Qi Long Yan Song liang-Ping li Department of Gastroenterology Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuanP.R.China Department of Gastroenterology The Affiliated Hospital of Southwest Medical UniversityLuzhouSichuanP.R.China Department of Gastroenterology Xinqiao HospitalThird Military Medical UniversityChongqingP.R.China Department of Gastroenterology Suining Central HospitalSuiningSichuanP.R.China

Background:In colonoscopy screening for colorectal cancer,human vision limitationsmay lead to highermiss rate of lesions;artificial intelligence(AI)assistance has been demonstrated to improve polyp ***,there still lacks direct evidence to demonstrate whether AI is superior to trainees or experienced nurses as a second observer to increase adenoma detection during *** this study,we aimed to compare the effectiveness of assistance fromAI and human observer during ***:A prospective multicenter randomized study was conducted from 2 September 2019 to 29 May 2020 at four endoscopy centers in *** patients were randomized to either computer-aided detection(CADe)-assisted group or observer-assisted *** primary outcome was adenoma per colonoscopy(APC).Secondary outcomes included polyp per colonoscopy(PPC),adenoma detection rate(ADR),and polyp detection rate(PDR).We compared continuous variables and categorical variables by using R studio(version 3.4.4).Results:A total of 1,261(636 in the CADe-assisted group and 625 in the observer-assisted group)eligible patients were ***(0.42 vs 0.35,P=0.034),PPC(1.13 vs 0.81,Plinical outcome of colonoscopy and reduce disturbance to routine practice(*** No.:ChiCTR1900025235).

关键词： artificial intelligence colon cancer screening adenoma early detection computer-aided detection

A randomized controlled trial of postoperative tumor lysate-pulsed dendritic cells and cytokine-induced killer cells immunotherapy in patients with localized and locally advanced renal cell carcinoma

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Chinese Medical Journal 2012年第21期125卷 3771-3777页

作者： ZHAN Hai-lun GAO Xin pu Xiao-yong li Wei li Zhi-jian zhou Xiang-fu QIU jian-guang Department of Urology Third Affiliated Hospital of Sun Yat-senUniversity Guangzhou.Guangdong 510630 China Department of Urology Zhongshan City People's HospitalZhongshan Guangdong 528403 China

Background It remains a challenge to inhibit the local recurrence or distant metastasis of localized or locally advanced renal cell carcinoma （RCC） after surgical resection. We investigated the feasibility, safety and efficacy of immunotherapy using autologous tumor lysate （TL）-pulsed dendritic cells （DCs） and cytokine-induced killer （CIK） cells in patients with localized or locally advanced RCC.

关键词： renal cell carcinoma " dendritic cells " cytokine-induced killer cells " immunotherapy

Large area^(3)He tube array detector with modular design for multi‑physics instrument at CSNS

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Nuclear Science and Techniques 2023年第1期34卷 1-12页

作者： lin Zhu jian‑Rong zhou Yuan‑guang Xia liang Xiao Hong Luo Xiao‑Juan zhou Wen‑Qin Yang Bei‑Ju guan Xing‑Fen jiang Yan‑Feng Wang Hong Xu Hai‑Yun Teng li‑Xin Zeng Jia‑Jie li Lei Hu Ke zhou yong‑Xiang Qiu Pei‑Xun Shen Jun Xu li‑jiang liao Xiao‑Zhuang Wang Gui‑An Yang Huai‑Chan Chen Ju‑Ping Xu Zhi‑Duo li Song‑lin Wang jian Zhuang Yu‑Bin Zhao Jun‑Rong Zhang Wen Yin Zhi‑Jia Sun Yuan‑Bo Chen Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Spallation Neutron Source Science Center Dongguan 523803China University of Chinese Academy of Sciences Beijing 100049China

The multi-physics instrument(MPI)is the first user cooperative instrument at the China Spallation Neutron Source(CSNS).It was designed to explore the structures of complex materials at multiple scales based on the neutron total scattering *** imposes the requirements for the detector,including a high detection efficiency to reduce the measurement time and a large solid angle coverage to cover a wide range of momentum *** satisfy these demands,a large-area array of 3He-filled linear position-sensitive detectors(LPSDs)was constructed,each with a diameter of 1 inch and pressure of 20 *** uses an orbicular layout of the detector and an eight-pack module design for the arrangement of 3He LPSDs,covering a range of scattering angles from 3°to 170°with a total detector area of approximately 7 *** detector works in air,which is separated from the vacuum environment to facilitate installation and *** characteristics of the MPI detector were investigated through Monte Carlo(MC)simulations using Geant4 and experimental *** results suggest that the detectors are highly efficient in the wavelength range of the MPI,and an efficiency over 25%is achievable for above 0.1 A neutrons.A minimal position resolution of 6.4 mm full width at half maximum(FWHM)along the tube length was achieved at a working voltage of 2200 V,and a deviation below 2 mm between the real and measured positions was attained in the beam *** detector module exhibited good consistency and an excellent counting rate capacity of up to 80 kHz,which satisfied the requirements of experiments with a high event *** of its operation over the past year have shown that the detector works steadily in sample experiments,which allows the MPI to serve the user program successfully.

关键词： Neutron detector LPSD Position resolution Counting rate capacity

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旋流煤粉燃烧的一维综合数值模拟

燃料化学学报 2002年第2期30卷 124-129页

作者：张健朱成凯普勇周力行清华大学工程力学系北京100084

发展了综合考虑气固两相旋流流动、气相燃烧、颗粒相变与燃烧及两相辐射传热的旋流煤粉燃烧一维数学模型 ,给出了气固两相能量方程中颗粒相变源项的计算表达式。应用这一模型对涡旋燃烧炉环形通道内多组工况下的旋流气体燃烧和煤粉燃... 详细信息

发展了综合考虑气固两相旋流流动、气相燃烧、颗粒相变与燃烧及两相辐射传热的旋流煤粉燃烧一维数学模型 ,给出了气固两相能量方程中颗粒相变源项的计算表达式。应用这一模型对涡旋燃烧炉环形通道内多组工况下的旋流气体燃烧和煤粉燃烧进行了数值模拟 ,计算得到的炉内温度分布和燃烧效率与实验数据基本相符。表明本文建立的模型可用于旋流煤粉燃烧的一维综合数值模拟。

关键词：旋流煤粉燃烧一维数值模拟涡旋燃烧炉环形通道