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Analysis of Simple Sequence Repeats Information from Floral Expressed Sequence Tags Resources of Papaya (<i>Carica papaya</i>L.)

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American Journal of Plant Sciences 2017年第9期8卷 2315-2331页

作者： priyanka priyanka Dileep Kumar Anurag Yadav Kusum Yadav U. N. Dwivedi Department of Biochemistry University of Lucknow Lucknow India College of Basic Sciences & Humanities Sardarkrushinagar Agricultural University Dantiwada Banaskantha India

Papaya (Carica papaya L.) is one of the most economically, medicinally and nutritionally important tropical fruit crops. Expressed sequence tags (ESTs) derived simple sequence repeat (SSR) markers are more valuable as they are derived from conserved genic portion. Development of EST-SSRs markers through in silico approach is cheaper, less time consuming and labour-intensive. In this study, we aimed to mine SSRs and developed EST-SSR primers from papaya floral ESTs. A total of 75,846 papaya floral ESTs were downloaded from public database National Centre for Biotechnology Information (NCBI). A total of 26,039 floral unigenes (7961 contigs and 18,078 singletons) were generated after assembly of these ESTs. From these floral unigenes, 433,782 perfect SSRs, 204,968 compound SSRs and 6061 imperfect SSRs were mined, respectively. In perfect SSRs, mononucleotide repeats were most abundant (94.7%) followed by tri- (3.1%) and di-nucleotide repeats (1.7%). The frequencies of tetra-, hexa- and penta-nucleotide repeats accounted for only (0.17%), (0.04%) and (0.03%), respectively. In mononucleotide repeats, the most abundant motif was A/T (69.3%) and in di- and tri-nucleotide repeats were AG/CT (61%) and AAG/CTT (31%), respectively. In imperfect SSRs, mononucleotide repeats (56.5%) were most abundant. 176 different types of motifs were identified. A total of 3807 primer pairs for floral papaya ESTs were successfully designed. These developed EST-SSR primers are being used for the genetic improvement of papaya such as study of cross-transferability across genera/species, evaluation of genetic diversity, and identification of sex-specific markers. These EST derived SSRs can also be used in filling gaps in existing linkage maps in papaya.

关键词： Papaya (Carica papaya L.) In Silico Simple Sequence Repeats Expressed Sequence Tags (ESTs) SSR Mining EST-SSR SSR Motifs Primer Pairs

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Role of self-expanding metal stents in patients with malignant colorectal obstruction: A systematic review and meta-analysis

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World Journal of Meta-Analysis 2015年第6期3卷 232-253页

作者： Nirav Thosani Subhas Banerjee Vikesh Khanijow Bhavana Rao priyanka priyanka Atilla Ertan Sushovan Guha Division of Gastroenterology and Hepatology Stanford University School of Medicine Division of Gastroen- terology Hepatology and Nutrition Department of Internal Medicine the University of Texas Health Medical School at Houston Ertan Digestive Disease Center Memorial Hermann Hospital--TMC

AIM: To assess the safety and efficacy of self-expandable metal stents(SEMSs) for malignant colorectal obstruction. METHODS: Data regarding technical success, clinical success, and procedure related complications were collected from included studies. Der Simonian-Laird random effects model was used to generate the overall outcome. Thirty international studies with a total of 2058 patients with malignant colorectal obstruction were included. RESULTS: The technical and clinical success rates for SEMS placement were 94%(95%CI: 92-96) and 91%(95%CI: 88-93), respectively. Overall complication rate for SEMS was 23%(95%CI: 18-29). Stent migration8%(95%CI: 6-10) and stent obstruction 8%(95%CI: 6-11) were the most common complications, followed by perforation 5%(95%CI: 4%-7%). Surgical or endoscopic re-interventions were needed in 14%(95%CI: 10-18) of patients. Endoscopic repeat stent placement was required in 8%(95%CI: 6-10), while surgical intervention was needed in 6%(95%CI: 4-8).CONCLUSION: SEMS are effective when used as palliation or bridge to surgery for malignant colorectal obstruction with high technical and clinical success. About 14% of patients require repeat endoscopic or surgical intervention for stent failure or to manage stent related complications.

关键词： Metal stent Colorectal cancer Colon cancer Rectal cancer Intestinal obstruction Bowel obstruction Malignant obstruction Colonic obstruction

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Small molecule inhibitor DDQ-treated hippocampal neuronal cells show improved neurite outgrowth and synaptic branching

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Neural Regeneration Research 2025年第9期20卷 2624-2632页

作者： Jangampalli Adi Pradeepkiran priyanka Rawat Arubala P.Reddy Erika Orlov PHemachandra Reddy Department of Internal Medicine Texas Tech University Health Sciences CenterLubbockTXUSA Department of Nutritional Sciences College of Human SciencesTexas Tech UniversityLubbockTXUSA Department of Pharmacology&Neuroscience Texas Tech University Health Sciences CenterLubbockTXUSA Department of Neurology Texas Tech University Health Sciences CenterLubbockTXUSA Department of Speech Language and Hearing SciencesTexas Tech University Health Sciences CenterLubbockTXUSA Department of Public Health Texas Tech University Health Sciences CenterLubbockTXUSA

The process of neurite outgrowth and branching is a crucial aspect of neuronal development and *** and dendrites,sometimes referred to as neurites,are extensions of a neuron's cellular body that are used to start *** we explored the effects of diethyl(3,4-dihydroxyphenethylamino)(quinolin-4-yl)methylphosphonate(DDQ)on neurite developmental features in HT22 neuronal *** this work,we examined the protective effects of DDQ on neuronal processes and synaptic outgrowth in differentiated HT22cells expressing mutant Tau(mTau)*** investigate DDQ chara cteristics,cell viability,biochemical,molecular,western blotting,and immunocytochemistry were *** outgrowth is evaluated through the segmentation and measurement of neural *** neural processes can be seen and measured with a fluorescence microscope by manually tracing and measuring the length of the neurite *** neuronal processes can be observed and quantified with a fluorescent microscope by manually tracing and measuring the length of the neuronal ***-treated mTau-HT22 cells(HT22 cells transfected with cDNA mutant Tau)were seen to display increased levels of synaptophysin,MAP-2,andβ-***,we confirmed and noted reduced levels of both total and p-Tau,as well as elevated levels of microtubule-associated protein 2,β-tubulin,synaptophysin,vesicular acetylcholine transporter,and the mitochondrial biogenesis protein-pe roxisome prolife rator-activated receptor-gamma coactivator-1α.In mTa u-expressed HT22 neurons,we observed DDQ enhanced the neurite characteristics and improved neurite development through increased synaptic *** findings conclude that mTa u-HT22(Alzheimer's disease)cells treated with DDQ have functional neurite developmental chara *** key finding is that,in mTa u-HT22 cells,DDQ preserves neuronal structure and may even enhance nerve development function with mTa u inhibition.

关键词： diethyl(3 4-dihydroxyphenethylamino)(quinolin-4-yl)methylphosphonate(DDQ) hippocampal neuronal cells HT22 neurite outgrowth neuronal development small molecule

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Bianchi Type-Ⅱ Inflationary Models with Stiff Matter and Decaying Cosmological Term

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Chinese Physics Letters 2014年第7期31卷 27-31页

作者： Shri Ram, priyanka Department of Mathematical Sciences Indian Institute of Technology Banaras Hindu University Varanasi 221 005 India

We deal with Einstein＇s field equations with a time-decaying cosmological term of the forms （i） ∧=β（a/a）＋ α/a62 and （ii）∧ = α/a^2, where a is the average scale factor of the universe, α and β are constants for a spatially homogeneous and anisotropic LRS Bianchi type-Ⅱ spacetime. Exact solutions of the field equations for stiff matter are obtained by applying a special law of variation for the Hubble parameter. Anisotropic cosmological models are presented with a constant negative deceleration parameter which corresponds to the accelerated phase of the present universe. The cosmological constant A is obtained as a decreasing function of time that is approaching a small positive value at the present epoch, which is corroborated by the consequences from recent supernovae Ia observations. The physical and kinematical behaviors of the models are also discussed.

关键词： Anisotropy

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Seismic analysis of nailed vertical excavation using pseudo-dynamic approach

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Earthquake Engineering and Engineering Vibration 2016年第4期15卷 621-631页

作者： Piyush Sarangi priyanka Ghosh Department of Civil Engineering Indian Institute of Technology

An attempt has been made to study the behavior of nailed vertical excavations in medium dense to dense cohesionless soil under seismic conditions using a pseudo-dynamic approach. The effect of several parameters such as angle of internal friction of soil （φ）, horizontal （kh） and vertical （kv） earthquake acceleration coefficients, amplification factor （fa）, length of nails （L）, angle of nail inclination （a） and vertical spacing of nails （S） on the stability of nailed vertical excavations has been explored. The limit equilibrium method along with a planar failure surface is used to derive the formulation involved with the pseudo-dynamic approach, considering axial pullout of the installed nails. A comparison of the pseudo-static and pseudo-dynamic approaches has been established in order to explore the effectiveness of the pseudo-dynamic approach over pseudo-static analysis, since most of the seismic stability studies on nailed vertical excavations are based on the latter. The results are expressed in terms of the global factor of safety （FOS）. Seismic stability, i.e., the FOS of nailed vertical excavations is found to decrease with increase in the horizontal and vertical earthquake forces. The present values of FOS are compared with those available in the literature.

关键词： earthquake factor of safety pseudo-dynamic approach soil nailing vertical excavation

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Using Markov chains to predict the natural progression of diabetic retinopathy

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International Journal of Ophthalmology(English edition) 2015年第1期8卷 132-137页

作者： priyanka Srikanth Jaslok Hospital & Research Centre

AIM: To study the natural progression of diabetic retinopathy in patients with type 2 ***: This was an observational study of 153 cases with type 2 diabetes from 2010 to 2013. The state of patient was noted at end of each year and transition matrices were developed to model movement between years. Patients who progressed to severe non-proliferative diabetic retinopathy(NPDR) were *** Chains and Chi-square test were used for statistical ***: We modelled the transition of 153 patients from NPDR to blindness on an annual basis. At the end of year 3, we compared results from the Markov model versus actual data. The results from Chi-square test confirmed that there was statistically no significant difference(P =0.70) which provided assurance that the model was robust to estimate mean sojourn times. The key finding was that a patient entering the system in mild NPDR state is expected to stay in that state for 5y followed by 1.07 y in moderate NPDR, be in the severe NPDR state for 1.33 y before moving into PDR for roughly8 y. It is therefore expected that such a patient entering the model in a state of mild NPDR will enter blindness after 15.29 ***: Patients stay for long time periods in mild NPDR before transitioning into moderate ***, they move rapidly from moderate NPDR to proliferative diabetic retinopathy(PDR) and stay in that state for long periods before transitioning into blindness.

关键词： diabetes mellitus diabetic retinopathy Markov Chains Chi-square test transition probability matrix

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The network of cytokines,receptors and transcription factors governing the development of dendritic cell subsets

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Protein & Cell 2011年第8期2卷 620-630页

作者： priyanka Sathe Li Wu Walter and Eliza Hall Institute 1G Royal ParadeParkvilleVictoria 3050Australia Department of Medical Biology University of MelbourneAustralia Tsinghua University School of Medicine Beijing 100084China

The pathways leading to the development of different dendritic cell(DC)subsets have long been *** recent years,a number of precursors on the route to DC development,both under steady state and inflammatory conditions,have been described,and the nature of these pathways is becoming *** addition,the development of various knockout mouse models and an in vitro system modelling DC development have revealed the role of numerous cytokines and transcription factors that influence DC ***,we review recent findings on the factors important in DC development in the context of the developmental pathways that have been described.

关键词： dendritic cell cytokines receptors transcription factors

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Transcriptional co-activators: emerging roles in signaling pathways and potential therapeutic targets for diseases

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Signal Transduction and Targeted Therapy 2023年第12期8卷 5686-5726页

作者： priyanka Dey Talukdar Urmi Chatterji Cancer Research Laboratory Department of ZoologyUniversity of Calcutta35 Ballygunge Circular RoadKolkata 700019 West BengalIndia

Specific cell states in metazoans are established by the symphony of gene expression programs that necessitate intricate synergic interactions between transcription factors and the *** of these regulatory molecules is associated with cell state transitions,which in turn is accountable for diverse maladies,including developmental disorders,metabolic disorders,and most significantly,cancer.A decade back most transcription factors,the key enablers of disease development,were historically viewed as‘undruggable’;however,in the intervening years,a wealth of literature validated that they can be targeted indirectly through transcriptional co-activators,their confederates in various physiological and molecular *** co-activators,along with transcription factors,have the ability to initiate and modulate transcription of diverse genes necessary for normal physiological functions,whereby,deregulation of such interactions may foster tissue-specific disease ***,it is essential to analyze how these co-activators modulate specific multilateral processes in coordination with other *** proposed review attempts to elaborate an in-depth account of the transcription co-activators,their involvement in transcription regulation,and context-specific contributions to pathophysiological *** review also addresses an issue that has not been dealt with in a comprehensive manner and hopes to direct attention towards future research that will encompass patient-friendly therapeutic strategies,where drugs targeting co-activators will have enhanced benefits and reduced side *** insights into currently available therapeutic interventions and the associated constraints will eventually reveal multitudes of advanced therapeutic targets aiming for disease amelioration and good patient prognosis.

关键词： therapeutic eventually potential

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A novel strategy for automatic test data generation using soft computing technique

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Frontiers of Computer Science 2015年第3期9卷 346-363页

作者： priyanka CHAWLA Inderveer CHANA Ajay RANA Computer Science and Engineering Department Thapar University Patiala 147004 India Amity School of Engineering Amity University Noida 201301 India

Software testing is one of the most crucial and analytical aspect to assure that developed software meets pre- scribed quality standards. Software development process in- vests at least 50% of the total cost in software testing process. Optimum and efficacious test data design of software is an important and challenging activity due to the nonlinear struc- ture of software. Moreover, test case type and scope deter- mines the quality of test data. To address this issue, software testing tools should employ intelligence based soft comput- ing techniques like particle swarm optimization （PSO） and genetic algorithm （GA） to generate smart and efficient test data automatically. This paper presents a hybrid PSO and GA based heuristic for automatic generation of test suites. In this paper, we described the design and implementation of the proposed strategy and evaluated our model by performing ex- periments with ten container classes from the Java standard library. We analyzed our algorithm statistically with test ad- equacy criterion as branch coverage. The performance ade- quacy criterion is taken as percentage coverage per unit time and percentage of faults detected by the generated test data. We have compared our work with the heuristic based upon GA, PSO, existing hybrid strategies based on GA and PSO and memetic algorithm. The results showed that the test case generation is efficient in our work.

关键词： software testing particle swarm optimization,genetic algorithm soft computing test data generation

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Exogenous Peroxidase Mitigates Cadmium Toxicity, Enhances Rhizobial Population and Lowers Root Knot Formation in Rice Seedlings

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Rice science 2021年第2期28卷 166-177页

作者： priyanka SINGH Chitra POKHARIA Kavita SHAH Department of Biochemistry Faculty of ScienceBanaras Hindu UniversityVaranasi 221005India Environmental Biotechnology Laboratory Department of Environment and Sustainable DevelopmentInstitute of Environment and Sustainable DevelopmentBanaras Hindu UniversityVaranasi 221005India

Soil cadmium (Cd) causes toxicity and oxidative stress, alters biochemical processes and rootknot formation in rice. Irrigation of exogenous peroxidase (POX) together with its co-substrate H2O2(POXRice + H2O2), is likely to have protective effect upon the biochemical and nodular changes in ricegrown in Cd-rich soil. Exposure to Cd concentration of 1.00 mg/L increased oxidative stress, loss of cellviability, electrolyte leakage and root knot formation, whereas it significantly lowered the chlorophyll leveland rhizobium growth in rice. Irrigation of exogenous POXRice + H2O2 to Cd-stressed rice seedlingsreversed the Cd-induced alterations in rice to levels similar in control (non-stressed) seedlings. Resultsprovided strong evidence of exogenous POXRice + H2O2-mediated reversal and restoration of physiologicaland biochemical processes as well as increased resistance of rice seedlings to root knot *** with POXRice + H2O2 appeared to contribute towards bringing normoxic conditions in the otherwisehypoxic soil environment by enhancing the O2 in pot-experiments due to reduced Cd uptake, enhancedmineral homeostasis of essential elements viz. P, Fe, Mo, Mg and Mn for maintenance of root architecturedamaged by lipid peroxidation and reduction in oxidative stress by reducing Cd-induced reactive oxygenspecies generation. Therefore, the mitigation of Cd-toxicity in rice through this novel approach appeared tobe a promising mode to limit Cd-uptake, modulate protective and tolerance mechanisms for sustainablerice yield in Cd-contaminated rice-croplands and prevent nematode attack in rice, however, more detailedstudies are needed prior to large scale applications.

关键词： cadmium peroxidase rice rhizobium root knot reactive oxygen species antioxidant enzyme

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