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Identification,development,and application of cross-species intron-spanning markers in lentil(Lens culinaris Medik.)

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The Crop Journal 2018年第3期6卷 299-305页

作者： Debjyoti Sen gupta Jitendra Kumara Sunanda gupta Sonali Dubey priyanka gupta Narendra Pratap Singh Gaurav Sablok Division of Crop Improvement ICAR-Institute of Pulses ResearchKanpur 208024UPIndia Division of Plant Biotechnology ICAR-Institute of Pulses ResearchKanpur 208024UPIndia Department of Biodiversity and Molecular Ecology Research and Innovation CentreFondazione Edmund MachVia E.Mach 138010 San Michele all'AdigeTrentoItaly

Lentil(Lens culinaris Medik) is one of the most important food legumes in the world. The use in lentil of molecular marker-assisted breeding is limited, owing to the low availability of polymorphic markers. In the present study, we developed a set of polymorphic intron-spanning markers(ISMs) using a cross-species mapping approach. In this approach, putative unique transcripts(PUTs) of L. culinaris were mapped onto the Medicago truncatula genome, exploiting its closeness with the lentil genome. Spliced alignment of the PUTs resulted in a total of 25,717 alignments, allowing the development of 1703 ISMs. From these, a subset of 105 ISMs were synthesized and validated with a 51% amplification success rate in 32 lentil genotypes. Of these ISMs, 40(74%) were polymorphic and generated 2–11 alleles per locus in a genetically diverse panel of 32 lentil genotypes including wild *** set of polymorphic ISMs along with their functional annotation data will be useful in lentil breeding.

关键词： Allele diversity Cross-species Genotypes Introns Lentil Molecular markers

Population structure analysis and determination of neurotoxin content in a set of grass pea (Lathyrus sativus L.) accessions of Bangladesh origin

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The Crop Journal 2018年第4期6卷 435-442页

作者： priyanka gupta Sripada M. Udupa Debjyoti Sen gupta Jitendra Kumar Shiv Kumar International Center for Agricultural Research in the Dry Areas Rabat Institute Rabat Morocco Department of Plant Sciences North Dakota State University Fargo ND 58105 USA ICAR-Indian Institute of Pulses Research Kanpur Uttar Pradesh 208024 India

Grass pea(Lathyrus sativus L.) is a crop that is considered one of the more resilient to climate change. With protein-rich seeds and leaves, it has strong potential as human food as well as animal feed and fodder. However, genetic improvement in this crop remains stagnant owing to the poor characterization of its genetic resources. In this study, we characterized 118 accessions of grass pea with 18 EST-SSR markers. A total of 118 accessions, 101 of L. sativus(100 cultivated accessions from Bangladesh and one wild accession) and 17 wild accessions of other Lathyrus species, were used. A total of 67 alleles were detected, with an average of 3.72 alleles per locus and average polymorphism information content of 0.52. A dissimilarity matrix was formed and hierarchical cluster analysis performed using the UPGMA method grouped genotypes into four main clusters. Cluster analysis based on the genetic dissimilarity revealed a clear grouping of the 100 cultivated and 18 wild accessions into four main groups. Group I consisted of 20 accessions with high β-N-oxalyl-L-α,β-diaminopropionic acid(β-ODAP) concentration. Of these 20 accessions, 17 were wild accessions. Only one wild accession(L. cicera) was clustered in group II, which contained 35 accessions. Most of the group II accessions contained low β-ODAP. Group III was represented by 34 accessions, many of them with high β-ODAP. Group IV consisted of 29 accessions, a few of which had very high β-ODAP concentrations. Analysis of molecular variance of the microsatellite data showed significantly higher values of molecular variance between(83%) than within(17%) populations. These characterized accessions will be useful in grass pea breeding programs.

关键词： Lathyrus Population structure EST-SSRβ-ODAP Genetic diversity

Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review

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World Journal of Gastroenterology 2016年第20期22卷 4824-4834页

作者： Mohammad Irshad priyanka gupta Dhananjay Singh Mankotia Mohammad Ahmad Ansari Clinical biochemistry division Department of Laboratory Medicine All India Institute of Medical Sciences New Delhi 110029 India

The present review describes the current status of multiplex quantitative real time polymerase chain reaction(q PCR) assays developed and used globally for detection and subtyping of hepatitis viruses in body fluids. Several studies have reported the use of multiplex q PCR for the detection of hepatitis viruses, including hepatitis A virus(HAV), hepatitis B virus(HBV), hepatitis C virus(HCV), hepatitis D virus(HDV), and hepatitis E virus(HEV). In addition, multiplex q PCR has also been developed for genotyping HBV, HCV, and HEV subtypes. Although a single step multiplex q PCR assay for all six hepatitis viruses, i.e., A to G viruses, is not yet reported, it may be available in the near future as the technologies continue to advance. All studies use a conserved region of the viral genome as the basis of amplification and hydrolysis probes as the preferred chemistries for improved detection. Based on a standard plot prepared using varying concentrations of template and the observed threshold cycle value, it is possible to determine the linear dynamic range and to calculate an exact copy number of virus in the specimen. Advantages of multiplex q PCR assay over singleplex or other molecular techniques in samples from patients with co-infection include fast results, low cost, and a single step investigation process.

关键词： Co-infection Viral genome Quantitative real-time polymerase chain reaction Genotyping techniques Serotyping Hepatitis viruses

An Empirical Model for the Classification of Diabetes and Diabetes_Types Using Ensemble Approaches

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Journal of Artificial Intelligence and Technology 2023年第4期3卷 181-186页

作者： Sushma Jaiswal priyanka gupta L.V.Narasimha Prasad Rajesh Kulkarni Guru Ghasidas Vishwavidyalaya KoniBilaspur(C.G.)India Department of CSE Institute of Aeronautical EngineeringHyderabadIndia MVSR Engineering College Nadargul HyderabadIndia

Diabetes is a hereditary disorder that interferes with human life at all *** is challenging for cells to absorb glucose from the bloodstream when an individual has *** two main subtypes of diabetes are type 1 diabetes and type 2 *** 1 diabetes develops when the pancreas cannot make enough insulin,whereas type 2 diabetes spreads due to insulin *** is a recurrent,and chronic illness that is *** modern healthcare systems,disease detection technology is *** diabetes in its early stages is crucial for initiating timely treatment and halting disease *** proposed method has the potential not only to forecast the likelihood of future diabetes onset but also to identify the specific type of diabetes a person may *** paper investigates a potential solution for a diabetes prediction model in light of the continually rising prevalence of diabetes among *** proposed framework is designed using two datasets:the Pima Indian dataset,which is used to forecast diabetes,and the DiabetesType dataset,which is used to identify the type of diabetes mellitus an individual *** research aims to apply machine learning classifiers and ensemble models,such as Bagging,Voting,Averaging,and Stacking,for diabetes *** this context,SMOTE(synthetic minority oversampling technique)and hyperparameter adjustment of the algorithms are considered and have substantially improved the *** developed heterogeneous ensemble model offers enhanced prediction rates with different performance *** the bagging technique,random forest attains a 96%accuracy rate,resulting in better predictions in the PID *** the DiabetesType dataset,the voting ensemble model provides a 98.5%accuracy *** study highlights that ensemble learning models are effective in predicting diabetes and can outperform earlier relevant studies.

关键词： classification diabetes mellitus ensemble learning PID random forest

Molecular basis of hepatocellular carcinoma induced by hepatitis C virus infection

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World Journal of Hepatology 2017年第36期9卷 1305-1314页

作者： Mohammad Irshad priyanka gupta Khushboo Irshad Clinical Biochemistry Division Department of Laboratory MedicineAll India Institute of Medical SciencesNew Delhi 110029India

Present study outlines a comprehensive view of published information about the underlying mechanisms operational for progression of chronic hepatitis C virus(HCV) infection to development of hepatocellular carcinoma(HCC). These reports are based on the results of animal experiments and human based studies. Although, the exact delineated mechanism is not yet established, there are evidences available to emphasize the involvement of HCV induced chronic inflammation, oxidative stress, insulin resistance, endoplasmic reticulum stress, hepato steatosis and liver fibrosis in the progression of HCV chronic disease to HCC. Persistent infection with replicating HCV not only initiates several liver alterations but also creates an environment for development of liver cancer. Various studies have reported that HCV acts both directly as well as indirectly in promoting this process. Whereas HCV related proteins, like HCV core, E1, E2, NS3 and NS5A, modulate signal pathways dysregulating cell cycle and cell metabolism, the chronic infection produces similar changes in an indirect way. HCV is an RNA virus and does not integrate with host genome and therefore, HCV induced hepatocarcinogenesis pursues a totally different mechanism causing imbalance between suppressors and proto-oncogenes and genomic integrity. However, the exact mechanism of HCC inducement still needs a full understanding of various steps involved in this process.

关键词： Hepatitis C virus Hepatocellular carcinoma Fibrosis Core NS5A Inflammation

Differential Effect of Aluminium on Enzymes of Nitrogen Assimilation in Excised Bean Leaf Segments

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Advances in Biological Chemistry 2016年第3期6卷 106-113页

作者： priyanka gupta Juliana Sarengthem Sonal Dhamgaye Rekha Gadre School of Biochemistry Devi Ahilya University Indore India

Aluminium is a potent toxicant in acidic soils. The present study was taken up to analyze the effects of Al on enzymes of nitrogen assimilation in excised bean (Phaseolus vulgaris) leaf segments so as to gain an insight of the mechanism involved. Supply of 0.001 to 0.1 mM AlCl3 to excised bean leaf segments affected the in vivo nitrate reductase activity differently in the presence of various inorganic nitrogenous compounds, being inhibited with 5 mM ammonium nitrate and 10 mM ammonium chloride but enhanced with 10 mM potassium nitrate. Al effect with 50 mM KNO3 varied with time, showing an increased activity at shorter duration, but decreased at longer duration. Al effect on in vivo NRA was dependent upon the nitrate concentration, thus, inhibiting it at 0, 1 and 50 mM KNO3, while increasing at 2 and 10 mM. Further, saturating and non-saturating effects were observed in the absence and presence of Al. Al supply influenced the in vitro NRA also, being increased at 10 mM, but decreased at 50 mM KNO3. Supply of Al to excised leaf segments substantially inhibited the glutamate dehydrogenase activity in the absence as well as presence of 5 mM NH4NO3 but increased the glutamate synthase activity. Inhibition of specific glutamate dehydrogenase activity by Al supply was also observed. However, specific glutamate synthase activity was increased in the presence of NH4NO3 only. The experiments demonstrated that effect of supply of aluminium on in vivo nitrate reductase activity depended upon nitrogenous source as well as nitrate concentration and it exerted reciprocal regulation of glutamate dehydrogenase and glutamate synthase activities, which depended upon N supply too.

关键词： Aluminium Effects Glutamate Dehydrogenase Glutamate Synthase Nitrate Reductase Bean Leaves Phaseolus vulgaris

Predictors of cerebrospinal fluid GeneXpert MTB/RIF positivity in patients with tuberculous meningitis:A prospective cohort study

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Asian Pacific Journal of Tropical Medicine 2024年第10期17卷 465-472页

作者： priyanka gupta Neeraj Kumar Ravindra Kumar Garg Hardeep Singh Malhotra Imran Rizvi Rajesh Verma Praveen Kumar Sharma Ravi Uniyal Shweta Pandey Amita Jain Parul Jain Department of Neurology King George’s Medical UniversityLucknowIndia Department of Microbiology King George’s Medical UniversityLucknowIndia

Objective:To identify the clinical,laboratory,and radiological markers that could predict a positive GeneXpert result in patients suspected of tuberculous ***:In this prospective,observational study,patients with tuberculous meningitis were systematically *** clinical,laboratory[including cerebrospinal fluid(CSF)microscopy,culture,and GeneXpert],and neuroimaging factors were *** participants were administered anti-tuberculous treatment and corticosteroids.A six-month follow-up was done to evaluate the ***:Of the 116 patients studied,54(46.6%)tested positive for GeneXpert,while 62(53.4%)were *** cranial nerve involvement(OR 3.71,95%CI 1.052-13.09,P=0.04)and the presence of basal exudates on neuroimaging(OR 5.22,95%CI 2.03-13.42,P=0.001)emerged as independent predictive factors for a positive GeneXpert result.A positive CSF GeneXpert result(P=0.002)and drug resistance(P=0.004)were significantly linked to adverse ***,a high score on the Medical Research Council stageⅢ(OR 5.64,95%CI 1.18-26.87,P=0.03)and elevated CSF cell counts(OR 1.002,95%CI 1.00-1.001,P=0.03)were identified as independent predictors of poor ***:Third cranial nerve involvement and the presence of basal exudates were significant indicators of a positive GeneXpert *** stageⅢdisability and elevated CSF cell counts predicted poor outcomes.

关键词： Tuberculous meningitis Mycobacterium tuberculosis Cerebrospinal fluid Ziehl-Neelsen staining GeneXpert

Role of topical heparin in the management of burns:experience in a district government hospital of Karnataka in South India

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Plastic and Aesthetic Research 2015年第1期2卷 111-114页

作者： Ashish gupta Thangam J.Verghese priyanka gupta Ashok K.gupta Department of Plastic and Microvascular Surgery SPS Apollo HospitalsLudhiana 141003PunjabIndia Department of Surgery Kasturba Medical CollegeMangalore 575001KarnatakaIndia Department of Pediatric Hematology and Oncology SPS Apollo HospitalsLudhiana 141003PunjabIndia Department of Plastic Surgery and Burns DMCHLudhiana 141001PunjabIndia

Aim:Heparin is a multifaceted compound with uses not only as an anticoagulant,but also as an anti-inflammatory,anti-allergenic,anti-histaminic,anti-serotonin,anti-proteolytic and neoangiogenic *** aim of the study was to study the effect of topical heparin in the management of second-degree ***:Between December 2005 and January 2007,60 consecutive patients,aged 10-60 years,with first-and second-degree thermal injuries ranging from 10%to 60%,were randomly enrolled in the study divided into a control group(C)and a heparin group(H)of 30 patients ***:Patients treated with topical heparin experienced statistically significant improved pain relief,faster healing,fewer complications and shorter hospital *** majority of the patients admitted were in an economically productive age group and were predominantly *** distribution between the two groups according to age,type of burns and extent of burns was not statistically ***:The current study demonstrates the efficacy of topical heparin in the treatment of first-and second-degree burns.

关键词： Benefits burns cost epidemiology heparin

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具有免疫刺激活性的药用植物概览(英文)

中西医结合学报 2011年第2期9卷 117-128页

作者： Sunil Kumar priyanka gupta Shalini Sharma Dinesh Kumar Institute of Pharmaceutical Sciences Kurukshetra University

人类使用药用植物治疗疾病可以追溯至远古时代。如今仍有很多人依赖传统医药进行日常保健。印度阿育吠陀医学及其他印度的古老文献中记载了多种植物的药用功效。这些药用植物中,有很多被认为能够提高人的机体对于外界感染的自然抵抗力,... 详细信息

人类使用药用植物治疗疾病可以追溯至远古时代。如今仍有很多人依赖传统医药进行日常保健。印度阿育吠陀医学及其他印度的古老文献中记载了多种植物的药用功效。这些药用植物中,有很多被认为能够提高人的机体对于外界感染的自然抵抗力,即很多药用植物具有免疫刺激活性。本文对这些具有免疫刺激活性的药用植物进行了综述。

关键词：佐剂,免疫植物,药用综述