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A rapid absorbance-based growth assay to screen the toxicity of oligomer Aβ42 and protect against cell death in yeast

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Neural Regeneration Research 2020年第10期15卷 1931-1936页

作者： prashant bharadwaj Ralph Martins Centre of Excellence for Alzheimer’s Disease Research and Care School of Medical and Health SciencesEdith Cowan UniversityWestern AustraliaAustralia School of Pharmacy and Biomedical Sciences Curtin Health and Innovation Research Institute(CHIRI)Faculty of Health SciencesCurtin UniversityWestern AustraliaAustralia School of Psychiatry and Clinical Neuroscience.University of Western Australia Western AustraliaAustralia School of Biomedical Science Macquarie UniversitySydneyNSWAustralia

Multiple lines of evidence show that soluble oligomer forms of amyloidβprotein(Aβ42)are the most neurotoxic species in the brain and correlates with the degree of neuronal loss and cognitive deficit in Alzheimer’s *** many studies have used mammalian cells to investigate oligomer Aβ42 toxicity,the use of more simple eukaryotic cellular systems offers advantages for large-scale screening *** have previously established and validated budding yeast,Saccharomyces cerevisiae to be a simple and a robust model to study the toxicity of Aβ.Using colony counting based methods,oligomeric Aβ42 was shown to induce dose-dependent cell death in *** have adapted this method for high throughput screening by developing an absorbance-based growth *** further validated the assay with treatments previously shown to protect oligomer Aβ42 induced cell death in mammalian and yeast *** assay offers a platform for studying underlying mechanisms of oligomer Aβ42 induced cell death using gene deletion/overexpression libraries and developing novel agents that alleviate Aβ42 induced cell death.

关键词： Alzheimer’s disease amyloid toxicity autophagy Aβ42 oligomer high-throughput screening latrepirdine neuroprotection yeast model

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Finding chemopreventatives to reduce amyloid beta in yeast

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Neural Regeneration Research 2016年第2期11卷 244-245页

作者： Ian G.Macreadie Costa Arvanitis prashant bharadwaj School of Science and Health Innovations Research Institute RMIT University Centre of Excellence for Alzheimer’s disease Research and Care School of Medical Sciences Edith Cowan University CHIRI Biosciences Research Precinct School of Biomedical Sciences Curtin University

Alzheimer’s disease（AD）is the most common form of age-related dementia with the latest report（World Alzheimer Report,2015）showing 46.8 million people are currently affected by *** number is expected to double every 20 years unless there is effective therapeutic *** 42 amino acid peptide known as amyloid beta（or Aβ）has been implicated as one of the main causative agents of AD after its discovery in plaques in 1985（Masters et al.,1985）.

关键词： Finding chemopreventatives to reduce amyloid beta in yeast AD

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