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Root Extract of Polygonum Hydropiper Alters the Expression of Rat Uterine Protein Profile in Presence and Absence of Ovary in-situ during Periimplantation Period:An Evidence on SDS-PAGE

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Journal of Reproduction and Contraception 2009年第4期20卷 223-236页

作者： pranjiv goswami Ajit Hazarika Hirendra N Sarma Reproduction Research Laboratory Department of Zoology(Center with Potential for Excellence in Biodiversity)Rajiv Gandhi University Department of Zoology Chaiduar College

Objective To study its effects on reproductive performance in albino rats.Methods The chromatographic fraction （CF） of crude methanolic extract of the herb has been subcutaneously administered to female albino rats. Experiments were carried out in adult cyclic females and oavriectomised （OVX） females during early gestation period. Uterine horns were collected following the respective treatment regimen to stud）, the protein profile in 15% gel SDS-PAGE.Results The CF induced changes in the expression of protein in rat uterus. New proteins have been expressed in uterus of adult cyclic females having ovary in-situ. The OVX females treated with CF showed altered uterine protein profile compared with that of OVX control and OVX estradiol-17β （E2） treated rats. The CF exerted its effect on expression of uterine protein during early gestation period in rats. While uterine proteins of CF treated females were similar to that of controls during preimplantation period; many of the proteins on day 6 of gestation have been found either missing or expressed in lesser intensity.Conclusion The root of Polygonum hydropiper contains potential compound（s） which can alter the reproductive performance of female rats modulating uterine protein expression.

关键词： Polygonum hydropiper periimplantation uterine protein SDS-PAGE

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Chromatographic Fraction of Polygonum hydropiper Root Modulates the Expression of Transforming Growth Factor-βI (TGF-βI) in Rat Uterus during Days 2-6 of Gestation

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Journal of Reproduction and Contraception 2011年第3期22卷 153-167页

作者： pranjiv goswami Ajit Hazarika Hirendra N Sarma Department of Zoology (Centre with Potential for Excellence in Biodiversity) Rajiv Gandhi University Department of Zoology Chaiduar College

Objective To examine the effect of the root extract on the growth factor during early pregnancy from day 2 to day 6 of gestation. Methods A purified form of root extract of Polygonum hydropiper was obtained by Thin Layer Chromatography （TLC）. Localization of transforming growth factor （TGF）- β1 was studied by immunohistochemistry and the expression of the mRNA of TGF- β1 was studied by Reverse transcriptase Polymerase Chain Reaction （RT-PCR） in uterine tissue from day 2 to day 6 of gestation. Results The TLC fraction treated in a dose of 5 mg/kg body weight per day showed decreased the number of implantation sites. The expression of TGF- β1 from day 2 to day 6 of gestation was altered by the TLCfraction and suppression of the mRNA transcript of the TGF- β1 on day 6 of gestation during implantation. Conclusion The root extract of Polygonum hydropiper in a dose of 5 mg/kg body weight per day decreased the expression of TGF- β1 in the implantation sites of rat uterus.

关键词： Polygonum hydropiper implantation sites TGF- β1 IHC RT-PCR Thin Layer Chromatography (TLC)

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