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An improved LC–MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study

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Journal of Pharmaceutical Analysis 2017年第2期7卷 95-102页

作者： Dhiraj M.Rathod Keyur R.Patel Hiren N.Mistri Arvind G.Jangid pranav S.Shrivastav Mallika Sanyal Department of Chemistry Kadi Sarva VishwavidyalayaGandhinagarAhmedabad-382015India Bioanalytical Research Department Accutest Research LabBodakdevAhmedabad-380059India Department of Chemistry School of SciencesGujarat UniversityAhmedabad-380009India Department of Chemistry St.Xavier's CollegeNavrangpuraAhmedabad-380009India

A highly sensitive and selective high performance liquid chromatography–tandem mass spectrometry method was developed and validated for the quantification of alverine（ALV） and its active metabolite, para hydroxy alverine（PHA）, in human plasma. For sample preparation, solid phase extraction of analytes was performed on Phenomenex Strata-X cartridges using alverine-d5 as the internal standard. The analytes were separated on Symmetry Shield RP18（150 mm×3.9 mm, 5 μm） column with a mobile phase consisting of acetonitrile and10 mM ammonium formate（65：35, v/v）. Detection and quantitation was done by electrospray ionization mass spectrometry in the positive mode using multiple reaction monitoring. The assay method was fully validated over the concentration range of 15.0–15,000 pg/mL for ALV and 30.0–15,000 pg/mL for PHA. The intra-day and inter-day accuracy and precision（% CV） ranged from 94.00% to 96.00% and 0.48% to 4.15% for both the analytes. The mean recovery obtained for ALV and PHA was 80.59% and 81.26%, respectively. Matrix effect,expressed as IS-normalized matrix factor ranged from 0.982 to 1.009 for both the analytes. The application of the method was demonstrated for the specific analysis of ALV and PHA for a bioequivalence study in 52 healthy subjects using 120 mg ALV capsules. The assay reproducibility was also verified by reanalysis of 175 incurred subject samples.

关键词： Alverine Para hydroxy alverine Liquid chromatography–tandem mass spectrometry Bioequivalence study Human plasma

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Oldest Asian Record of Snapping Shrimps(Malacostraca:Alpheidae)from the Kutch Basin,Western India and Associated Biota:Biostratigraphic,Paleoenvironmental and Paleoecological Significance

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Acta Geologica Sinica(English Edition) 2022年第6期96卷 1867-1883页

作者： Vivesh V.KAPUR Kamlesh KUMAR pranav J.PANDYA Amit KGHOSH Arindam CHAKRABORTY Anupam SHARMA Gaurav CHAUHAN M.G.THAKKAR Birbal Sahni Institute of Palaeosciences 53University RoadLucknow 226007India Department of Biology Shri R.R.Lalan CollegeBhujKachchh DistrictGujaratIndia Institute of Earth Sciences Academia SinicaTaipeiTaiwan 11529China Department of Earth and Environmental Science K.S.K.V.Kachchh UniversityBhujKachchh DistrictGujaratIndia

The oldest Asian record of alpheid shrimps,assigned to genus Alpheus,based on snapping claw fingertips from the Miocene Khari Nadi Formation in the Kutch Basin,western India reported herein,extends the fossil record of the family Alpheidae from Asia by~22 million *** early Miocene(Aquitanian)age is estimated based on the associated assemblage of calcareous nannofossils,Sphenolithus disbelemnos,Cyclicargolithus floridanus,and Reticulofenestra *** co-occurring microbiota includes bony fish otolith remains,identified as“genus Gobiidarum”,isolated teeth of Dasyatis rays,Sphyrna sharks,and teleosts,ctenoid and placoid scales,ostracods,belonging to the genera Paractinocythereis,Alocopocythere,Ruggieria,Aglaiocypris,Bairdoppilata,and echinoid *** together,the microfossil assemblage and data from chemical analyses using Energy Dispersive Spectroscopy,X-Ray Diffraction and Wavelength Dispersive X-Ray Fluorescence of host and associated lithologies suggests prevalence of a shallow(neritic)to coastal marine(intertidal)depositional *** present investigation also provides the oldest fossil evidence on the co-occurrence of Alpheus and gobiids(based on otoliths)that strongly advocates that the mutualistic association between these animal groups had developed by the Aquitanian.

关键词： biostratigraphy Crustacea Caridea Elasmobranchs Gobiidae invertebrate and vertebrate paleontology nannofossils Oligocene-Miocene Indian subcontinent

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Development of a sensitive and rapid method for quantitation of (S)-(-)- and (R)-(+)-metoprolol in human plasma by chiral LC–ESI–MS/MS

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Journal of Pharmaceutical Analysis 2014年第1期4卷 63-79页

作者： Primal Sharma Pritesh Contractor Swati Guttikar Daxesh P.Patel pranav S.Shrivastav Department of Chemistry School of Sciences Gujarat University Bioanalytical Research Department Veeda Clinical Research

A selective, sensitive and high throughput liquid chromatography-tandem mass spectro-metry （LC-ESI-MS/MS） method has been developed for separation and quantification of metoprolol enantiomers on a chiral Lux Amylose-2 （250 mm＆#215;4.6 mm, 5 mm） column. Solid phase extraction of （S）-（-）- and （R）-（t）-metoprolol and rac-metoprolol-d6 as an internal standard （IS） was achieved on Lichrosep DVB HL cartridges employing 200 mL human plasma. Both the analytes were chromatographically separated with a resolution factor of 2.24 using 15 mM ammonium acetate in water, pH 5.0 and 0.1% （v/v） diethyl amine in acetonitrile （50：50, v/v） as the mobile phase within 7.0 min. The precursor-product ion transitions for the enantiomers and IS were monitored in the multiple reaction monitoring and positive ionization mode. The method was validated over the concentration range of 0.500-500 ng/mL for both the enantiomers. Matrix effect was assessed by post-column analyte infusion experiment and the mean extraction recovery was greater than 94.0% for both the enantiomers at all quality control levels. The stability of analytes was evaluated in plasma and whole blood under different storage conditions. The method was successfully applied to a clinical study in 14 healthy volunteers after oral administration of 200 mg metoprolol tablet under fasting conditions. The assay reproducibility is shown by reanalysis of 68 incurred samples. The suitability of the developed method was assessed in comparison with＆amp;nbsp;different chromatographic methods developed for stereoselective analysis of metoprolol in biological matrices.

关键词： S-(-)-metoprolol R-(+)-metoprolol Chiral column Chromatographicseparation LC-ESI-MS/MS Human plasma

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Remdesivir, dexamethasone and angiotensin-converting enzyme inhibitors use and mortality outcomes in COVID-19 patients with concomitant troponin elevation

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世界心脏病学杂志:英文版(电子版) 2023年第9期15卷 427-438页

作者： Chukwuemeka A Umeh Heather Maoz Jessica Obi Ruchi Dakoria Smit Patel Gargi Maity pranav Barve Internal Medicine Hemet Global Medical CenterHemetCA 92543United States

BACKGROUND There are indications that viral myocarditis,demand ischemia,and renin-angio-tensin-aldosterone system pathway activation play essential roles in troponin elevation in coronavirus disease 2019(COVID-19)*** medications and steroids are used to treat viral myocarditis,but their effect in patients with elevated troponin,possibly from myocarditis,has not been *** To evaluate the effect of dexamethasone,remdesivir,and angiotensin-converting enzyme(ACE)inhibitors(ACEI)on mortality in COVID-19 patients with elevated *** Our retrospective observational study involved 1788 COVID-19 patients at seven hospitals in Southern California,United *** did a backward selection Cox multivariate regression analysis to determine predictors of mortality in our study ***,we did a Kaplan Meier survival analysis in the subset of patients with elevated troponin,comparing survival in patients that received dexamethasone,remdesivir,and ACEI with those that did *** The mean age was 66 years(range 20-110),troponin elevation was noted in 11.5%of the patients,and 29.9%*** patients'age[hazard ratio(HR)=1.02,P<0.001],intensive care unit admission(HR=5.07,P<0.001),and ventilator use(HR=0.68,P=0.02)were significantly associated with *** the subset of patients with elevated troponin,there was no statistically significant difference in survival in those that received remdesivir(0.07),dexamethasone(P=0.63),or ACEI(P=0.8)and those that did *** Although elevated troponin in COVID-19 patients has been associated with viral myocarditis and ACE II receptors,conventional viral myocarditis treatment,including antiviral and steroids,and ACEI did not show any effect on mortality in these patients.

关键词： Coronavirus disease 2019 Troponin elevation Remdesevir Ace inhibitor Steroids

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Determination of lercanidipine in human plasma by an improved UPLC–MS/MS method for a bioequivalence study

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Journal of Pharmaceutical Analysis 2016年第2期6卷 87-94页

作者： Darshan V.Chaudhary Daxesh P.Patel Priyanka A.Shah Jaivik V.Shah Mallika Sanyal pranav S.Shrivastav Department of Chemistry School of Sciences Gujarat University Navrangpura Ahmedabad Gujarat 380009 India Laboratory of Metabolism Center for Cancer Research National Cancer Institute National Institutes of Health Building 37 Room 3106 Bethesda MD 20892 USA Department of Chemistry St. Xavier's College Navrangpura Ahmedabad Gujarat 380009 India

An improved and reliable ultra-performance liquid chromatography/tandem mass spectrometry （UPLC- MS/MS） method has been developed and validated for the determination of lercanidipine in human plasma. Plasma samples with lercanidipine-d3 as an internal standard （IS） were prepared by solid phase extraction on Phenomenex Strata-X cartridges using 100 μL of human plasma. Chromatographic analysis was performed on UPLC BEH C18 （50 mm × 2.1 mm, 1.7 μm） column under isocratic conditions. Linear calibration curves were obtained over a wide dynamic concentration range of 0.010-20.0 ng/mL. Matrix effect was assessed by post-column infusion, post-extraction spiking and standard-line slope methods. The mean extraction recovery was 〉 94% for the analyte and IS. Inter-batch and intra-hatch precision （% CV） across five quality controls was 〈 5.8%. Bioequivalence study was performed with 36 healthy sub- jects after oral administration of 10 mg of lercanidipine and the assay reproducibility was evaluated by reanalysis of 133 incurred samples.

关键词： Lercanidipine UPLC-MS/MS Bioequivalence Solid phase extraction Human plasma

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Selective and rapid determination of raltegravir in human plasma by liquid chromatography–tandem mass spectrometry in the negative ionization mode

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Journal of Pharmaceutical Analysis 2015年第2期5卷 101-109页

作者： Ajay Gupta Swati Guttikar Priyanka A.Shah Gajendra Solanki pranav S.Shrivastav Mallika Sanyal Chemistry Department Kadi Sarva Vishwavidyalaya Sarva Vidyalaya Campus Sector 15/23 Bioanalytical Research Department Veeda Clinical Research Department of Chemistry School of Sciences Gujarat University Department of Chemistry St.Xavier’s College

A selective and rapid high-performance liquid chromatography–tandem mass spectrometry method was developed and validated for the quantification of raltegravir using raltegravir-d3 as an internal standard（IS）. The analyte and IS were extracted with methylene chloride and n-hexane solvent mixture from 100 mL human plasma. The chromatographic separation was achieved on a Chromolith RP-18 e endcapped C18（100 mm 4.6 mm） column in a run time of 2.0 min. Quantitation was performed in the negative ionization mode using the transitions of m/z 443.1-316.1 for raltegravir and m/z 446.1-319.0 for IS. The linearity of the method was established in the concentration range of 2.0–6000 ng/m *** mean extraction recovery for raltegravir and IS was 92.6% and 91.8%, respectively, and the IS-normalized matrix factors for raltegravir ranged from 0.992 to 0.999. The application of this method was demonstrated by a bioequivalence study on 18 healthy subjects.

关键词： Raltegravir LC–ESI–MS/MS Negative ionization mode Human plasma Bioequivalence study

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Variable rate fertilizer application technology for nutrient management:A review

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International Journal of Agricultural and Biological Engineering 2023年第4期16卷 11-19页

作者： pranav Pramod Pawase Sachin Madhukar Nalawade Girishkumar Balasaheb Bhanage Avdhoot Ashok Walunj Pravin Bhaskar Kadam Anil G Durgude Mahesh R Patil Department of Farm Machinery and Power Engineering Dr.ASCAETMPKVRahuri(MS)India CAAST-CSAWM MPKVRahuri(MS)India Department of Soil Science and Agricultural Chemistry MPKVRahuri(MS)India Department of Statistics MPKVRahuri(MS)India

The efficient and effective application of fertilizers to crops is a major ***,constant rate or equal dose of fertilizer is applied to each *** rate fertilizer application across entire field can result in over or under incorporation of *** application is influenced by soil parameters as well as geographical variation in the *** nutrient management depends on selection of nutrient,application rate and placement of nutrient at the optimal distance from the crop and soil *** rate technology(VRT)is an input application technology that allows for the application of inputs at a certain rate,time,and place based on soil properties and spatial variation in the field or *** are two approaches for implementing VRT,one is sensor based and another is map *** sensor based approach;with suitable sensors,measures the soil and crop characteristics on-the-go calculating the amount of nutrients required per unit area/plant and micro controlling unit which uses suitable algorithms for controlling the flow of fertilizer with required amount of *** map based approach;Grid sampling and soil analysis are used to create a prescription *** to the soil and crop conditions,the microcontroller regulates the desired application *** sensor-based VRT system includes a fertilizer tank,sensors,GPS,microcontroller,actuators,and other components,whereas the map-based system does not require an on-the-go *** approaches of VRT for fertilizer application in orchards and field crops are reviewed in this *** use of this advance technology surely increases the fertilizer use efficiency;improve crop yield and profitability with reduced environment impacts.

关键词： nutrient sensor prescription map spatial variation VRT

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Liquid chromatography-tandem mass spectrometry method for simultaneous determination of albendazole and albendazole sulfoxide in human plasma for bioequivalence studies

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Journal of Pharmaceutical Analysis 2016年第4期6卷 226-234页

作者： Dhiraj M.Rathod Keyur R.Patel Hiren N.Mistri Arvind G.Jangid pranav S.Shrivastav Mallika Sanyal Department of Chemistry Kadi Sarva Vishwavidyalaya Bioanalytical Research Department Accutest Research Lab Department of Chemistry School of SciencesGujarat University Department of Chemistry St.Xavier's College

An improved high performance liquid chromatography-tandem mass spectrometry（LC-MS/MS） method has been developed for sensitive and rapid determination of albendazole（ABZ） and its active metabolite,albendazole sulfoxide（ABZSO）,in the positive ionization *** method utilized solid phase extraction（SPE） for sample preparation of the analytes and their deuterated internal standards（ISs） from100 μL human *** chromatography was carried out on Hypurity C_（18） column using acetonitrile-2.0 mM ammonium acetate,pH 5.0（80：20,v/v） as the mobile *** assay exhibited a linear response over the concentration range of 0.200-50.0 ng/mL for ABZ and 3.00-600 ng/mL for *** recoveries of the analytes and ISs ranged from 86.03%-89.66%and 89.85%-98.94%,*** effect,expressed as IS-normalized matrix factors,ranged from 0.985 to 1.042 for the both *** method was successfully applied for two separate studies in healthy subjects using single dose of 400 mg conventional tablets and 400 mg chewable ABZ tablets,respectively.

关键词： Albendazole Albendazole sulfoxide LC-MS/MS Solid phase extraction Bioequivalence study

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Clostridium perfringens bacteremia caused by choledocholithiasis in the absence of gallbladder stones

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World Journal of Gastroenterology 2012年第39期18卷 5632-5634页

作者： Antwan Atia Tejas Raiyani pranav Patel Robert Patton Mark Young Departments of Gastroenterology James H Quillen VA Medical CenterJohnsonMountain HomeTN 37615United States General Internal Medicine Quillen College of MedicineEast Tennessee State UniversityJohnsonMountain HomeTN 37615United States

A 67-years-old male presented with periumbilical abdominal pain, fever and jaundice. His anaerobic blood culture was positive for clostridium perfringens. Computed tomogram scan of the abdomen and abdominal ultrasound showed normal gallbladder and common bile duct (CBD). Subsequently magnetic resonance cholangiopancreaticogram showed choledocholithiasis. Endoscopic retrograde cholangiopancreaticogram-with sphincterotomy and CBD stone extraction was performed. The patient progressively improved with antibiotic therapy Choledocholithiasis should be considered as a source of clostridium perfringens bacteremia especially in the setting of elevated liver enzymes with cholestatic pattern.

关键词： Choledocholithiasis Clostridium perfrin-gens Bacteremia

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The epigallocatechin gallate derivative Y_6 reverses drug resistance mediated by the ABCB1 transporter both in vitro and in vivo

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Acta Pharmaceutica Sinica B 2019年第2期9卷 316-323页

作者： Yan Wen Ruiqiang Zhao pranav Gupta Yingfang Fan Yunkai Zhang Zhenguang Huang Xiaohui Li Yuangang Su Lijuan Liao Yu-An Xie Donghua Yang Zhe-Sheng Chen Gang Liang Department of Pharmaceutical Sciences College of Pharmacy and Health Sciences St.John's University Department of Pharmacy the First Affiliated Hospital of Guangxi Medical University Guangxi Colleges and Universities Key Laboratory of Biological Molecular Medicine Research Guangxi Medical University Department of Biochemistry and Molecular Biology School of Preclinical Medicine Guangxi Medical University Department of Hepatobiliary Surgery Zhujiang Hospital Southern Medical University Department of Biotechnology School of Preclinical Medicine Guangxi Medical University The Affiliated Tumor Hospital of Guangxi Medical University College of Pharmacy Guangxi Medical University

Previously, we reported that Y_6, a new epigallocatechin gallate derivative, is efficacious in reversing doxorubicin(DOX)–mediated resistance in hepatocellular carcinoma BEL-7404/DOX cells. In this study, we evaluated the efficacy of Y_6 in reversing drug resistance both in vitro and in vivo by determining its effect on the adenosine triphosphate-binding cassette protein B1 transporter(ABCB1 or P-glycoprotein, P-gp). Our results showed that Y_6 significantly sensitized cells overexpressing the ABCB1 transporter to anticancer drugs that are ABCB1 substrates. Y_6 significantly stimulated the adenosine triphosphatase activity of ABCB1. Furthermore, Y_6 exhibited a higher docking score as compared with epigallocatechin gallate inside the transmembrane domain of ABCB1. In addition, in the nude mousetumor xenograft model, Y_6(110 mg/kg, intragastric administration), in combination with doxorubicin(2 mg/kg, intraperitoneal injection), significantly inhibited the growth of BEL-7404/DOX cell xenograft tumors, compared to equivalent epigallocatechin gallate. In conclusion, Y_6 significantly reversed ABCB1-mediated multidrug resistance and its mechanisms of action may result from its competitive inhibition of the ABCB1 drug efflux function.

关键词： Epigallocatechin gallate(EGCG) 5,3’,4’,3″,4″,5″-6-O-ethylEGCG(Y6) Drug resistance Resistance reversal ABCB1 P-gp

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