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Peoples’ Perception and Conservation of Dactylorhiza hatagirea (D. Don) Soóin Manaslu Conservation Area, Central Nepal

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American Journal of Plant Sciences 2016年第12期7卷 1662-1672页

作者： Bikram Pandey Arbindra Timilsina Binita Pandey Chhabi Lal Thapa Kamal Bahadur Nepali pradeep Neupane resham Thapa Sunil Kumar Gaire Mohan Siwakoti Central Department of Botany Tribhuvan University Kirtipur Nepal Key Laboratory of Agricultural Water resources Center for Agricultural Resources Research Institute of Genetic and Developmental Biology The Chinese Academy of Sciences Shijiazhuang China University of Chinese Academy of Sciences Beijing China Central Department of Zoology Tribhuvan University Kirtipur Nepal National Botanical Garden Godawari Lalitpur Nepal

The present study analyzes the information and perception of the local community of Samagaun VDC, Manaslu Conservation Area Project (MCAP) regarding Dactylorhiza hatagirea (D. Don) Soó (Orchidaceae). We assessed the local peoples’ perception on its population status, its availability, factors causing its decline and management practices of this terrestrial orchids. A pre-designed questionnaire was used to gather information targeting the age group between 25 and 60 years (n = 75, 45 male and 30 female). Most of the informants (76%) believe that the abundance of this orchid is declining. Over grazing of domestic animals, over harvesting and lack of awareness among the local community were determined to be the major causes of decline of D. hatagirea in the study area. Protection measures as prescribed by the informants were control grazing, raising awareness among the individuals and sustainable harvestings for the long-term conservation of the species. Systematic management plans that incorporate the participation of local individuals and prioritization of their views will be applicable for the proper conservation of the species.

关键词： Local Perceptions Conservation and Management Orchid Dactylorhiza hatagirea Samagaun VDC Manaslu Conservation Area

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我国食品微生物定量风险评估的研究进展

食品科学 2015年第11期36卷 221-229页

作者：董庆利王海梅 pradeep K MALAKAr 刘箐宋筱瑜田明胜陆冉冉上海理工大学医疗器械与食品学院上海200093 英国食品研究所国家食品安全风险评估中心北京100021 上海市食品药品监督管理局执法总队上海200032 复旦大学公共卫生学院上海200032

近10余年来,我国政府重视食品安全并逐渐加强风险分析体系的构建和实施。根据历年来国家卫生与计生委的统计数据,表明由致病微生物导致的食物中毒发病率一直高于其他危害。食源性致病菌引起的食品安全风险是全球性问题,发展中国家面临... 详细信息

近10余年来,我国政府重视食品安全并逐渐加强风险分析体系的构建和实施。根据历年来国家卫生与计生委的统计数据,表明由致病微生物导致的食物中毒发病率一直高于其他危害。食源性致病菌引起的食品安全风险是全球性问题,发展中国家面临的情况更为严峻,因此加强我国微生物定量风险评估以减少与发达国家的差距,从国家层面上势在必行。特别是2009年我国颁布实施《食品安全法》和2011年成立国家食品安全风险评估中心(China National Center for Food Safety risk Assessment,CFSA)以来,已有不少针对国内具体食品致病菌情况开展的食品微生物定量风险评估(quantitative microbial risk assessment,QMrA)研究。本文对2000年至今我国已开展的QMrA研究,包括涉及的食品、致病菌、微生物预测模型、剂量-效应模型等进行详细综述。同时指出我国开展QMrA面临的技术性难题及解决方法,并对采用QMrA结果用于构建危害分析与关键控制点(Hazard Analysis and Critical Control Point,HACCP)和食品安全目标(food safety objective,FSO)以及执行目标(performance objective,PO)的应用前景进行探讨。建议加强风险评估与风险管理的互动交流、进一步完善风险监测、微生物限量制定和国际合作,在完善实施指南的基础上针对我国具体国情开展更多具有科学性和系统性的QMrA研究。

关键词：微生物定量风险评估致病菌预测微生物学

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Threats of Zika virus transmission for Asia and its Hindu-Kush Himalayan region

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Infectious Diseases of Poverty 2018年第1期7卷 387-393页

作者： Meghnath Dhimal Sushma Dahal Mandira Lamichhane Dhimal Shiva raj Mishra Khem BKarki Krishna Kumar Aryal Ubydul Haque Md Iqbal Kabir pradeep Guin Azeem Mehmood Butt Harapan Harapan Qi-Yong Liu Cordia Chu Doreen Montag David Alexander Groneberg Basu Dev Pandey Ulrich Kuch ruth Muller Nepal Health research Council(NHrC) Ramshah PathKathmanduNepal Institute of Occupational edicine Social Medicine and Environmental MedicineGoethe UniversityFrankfurt am MainGermany Faculty of Social Sciences Goethe UniversityFrankfurt am MainGermany The University of Queensland BrisbaneAustralia Department of Public Health Baldwin Wallace UniversityBereaOhioUSA Department of Epidemiology National Institute of Preventive and Social MedicineMinistry of Health and Family WelfareDhakaBangladesh Public Health Foundation of India GurgaonHaryanaIndia Centre for Environmental Health GurgaonHaryanaIndia Translational Genomics Laboratory Department of BiosciencesCOMSATS Institute of Information Technology(CIIT)Islamabad 45550Pakistan Medical research Unit School of MedicineSyiah Kuala UniversityBanda AcehIndonesia WHO Collaborating Centre for Vector Surveillance and Management SKLIDCCIDICDCChina CDCBeijingChina Centre for Environment and Population Health Griffith UniversityNathanQueenslandAustralia Barts and the London School of Medicine Centre for Primary Care and Public HealthQueen Mary University of LondonLondonUK Department of Health Services Ministry of HealthGovernment of NepalKathmanduNepal

Asia and its Hindu Kush Himalayan(HKH)region is particularly vulnerable to environmental change,especially climate and land use changes further influenced by rapid population growth,high level of poverty and unsustainable *** has been a hotspot of dengue fever and chikungunya mainly due to its dense human population,unplanned urbanization and *** an urban cycle,dengue virus(DENV)and chikungunya virus(CHIKV)are transmitted by Aedes aegypti and *** mosquitoes which are also competent vectors of Zika virus(ZIKV).Over the last decade,DENV and CHIKV transmissions by *** have extended to the Himalayan countries of Bhutan and Nepal and ZIKV could follow in the footsteps of these viruses in the HKH *** already established distribution of human-biting Aedes mosquito vectors and a naïve population with lack of immunity against ZIKV places the HKH region at a higher risk of *** of the countries in the HKH region have already reported ZIKV *** have documented an increasing threat of ZIKV in Asia and its HKH region because of the high abundance and wide distribution of human-biting mosquito vectors,climate change,poverty,report of indigenous cases in the region,increasing numbers of imported cases and a naïve population with lack of immunity against *** outbreak anywhere is potentially a threat ***,in order to ensure international health security,all efforts to prevent,detect,and respond to ZIKV ought to be intensified now in Asia and its HKH *** prepare for possible ZIKV outbreaks,Asia and the HKH region can also learn from the success stories and strategies adopted by other regions and countries in preventing ZIKV and associated *** future control strategies for DENV,CHIKV and ZIKV should be considered in tandem with the threat to human well-being that is posed by other emerging and re-emerging vector-borne and zoonotic diseases,and by the continuing urgent need to strengthen public

关键词： Aedes aegypti Aedes albopictus Dengue virus Chikungunya virus Hindu Kush Himalayas Mountain Poverty Zika virus

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同时到达脉冲分离器

电子信息对抗技术 1987年第4期 48-52页

作者： pradeep Wahi Zygmond Turski 顾耀平

电子战 IFM(瞬时测频接收机)中的同时到达信号未被人们重视,因而降低了截获概率。这些信号经过一条强色散延迟线传播即可在时域内得以分离。静磁波(MSW)具有固有的色散性,因此极可能直接在微波频率上解决向时到达信号分离的问题。本文... 详细信息

电子战 IFM(瞬时测频接收机)中的同时到达信号未被人们重视,因而降低了截获概率。这些信号经过一条强色散延迟线传播即可在时域内得以分离。静磁波(MSW)具有固有的色散性,因此极可能直接在微波频率上解决向时到达信号分离的问题。本文介绍一种采用色散延迟线的同时到达脉冲分离器,报道了静磁波色散延迟线的实验结果。

关键词：同时到达信号 IFM 色散延迟线信道化接收机非搜索式接收机色散特性电子战电子对抗信号分离色散性 MSW 时域分离截获概率

Finger Millet: An Alternative Crop for the Southern High Plains

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American Journal of Plant Sciences 2015年第16期6卷 2686-2691页

作者： Prasanna H. Gowda P. V. Vara Prasad Sangamesh V. Angadi Umesh M. rangappa pradeep Wagle USDA-ArS Grazinglands research Laboratory El Reno OK USA Department of Agronomy Kansas State University Manhattan KS USA Agriculture and Science Center New Mexico State University Clovis NM USA Department of Microbiology and Plant Biology and Center for Spatial Analysis University of Oklahoma Norman OK USA

In the Southern High Plains, dairies are expanding to take advantage of favorable climatic conditions. Currently, corn (Zea mays L.) and forage sorghum [Sorghum bicolor (L.) Moench] are the two major crops grown in the region to meet silage demands for the expanding dairy industry, but they have relatively large water requirements of about 840 and 690 mm, respectively, to achieve desirable results. With rising energy costs and declining water levels in the underlying Ogallala Aquifer, crops that use less water, like finger millet (Eleusine coracana (L.) Gaertn) could become alternate forage crops for dairies to corn or forage silage. In this study, we evaluated the adaptability of five finger millet accessions to the Southern High Plains and compared nutritional quality of their forage to that of corn and sorghum. results indicated that finger millet can be grown in the Southern High Plains. Comparison of nutrient composition has shown that the quality of finger millet is relatively higher than that of corn and sorghum in terms of calcium, potassium, and phosphorus levels in their forage. However, potential forage yield of most commonly grown corn and sorghum in the region is higher than that of finger millet. Therefore, finger millet may provide a unique opportunity to improve the dairy-fed silage quality by mixing it with corn or sorghum silage while meeting the growing regional forage demand. Further field research is needed to measure its water requirements in the Southern High Plains.

关键词： Ogallala Aquifer region Semi-Arid Forage Texas Panhandle

巴氏消毒及冷藏温度作用下副溶血弧菌菌株失活异质性的比较

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上海海洋大学学报 2020年第3期29卷 420-428页

作者：俞文英张昭寰钱慧刘海泉王敬敬 pradeep K MALAKAr 陈雪潘迎捷赵勇上海海洋大学食品学院上海201306 上海水产品加工及贮藏工程技术研究中心上海201306 农业农村部水产品贮藏保鲜质量安全风险评估实验室上海201306 上海德诺产品检测有限公司上海200436

温度是影响细菌生长与失活的关键因素,在食品生产中常用来控制致病微生物潜在的风险。但由于不同菌株间个体的差异,细菌在相同温度作用下呈现出不同的失活趋势,这种行为方式称为菌株的失活异质性,容易导致微生物风险控制的不确定性和变... 详细信息

温度是影响细菌生长与失活的关键因素,在食品生产中常用来控制致病微生物潜在的风险。但由于不同菌株间个体的差异,细菌在相同温度作用下呈现出不同的失活趋势,这种行为方式称为菌株的失活异质性,容易导致微生物风险控制的不确定性和变异性。比较了19株副溶血性弧菌(16株临床菌株和3株环境菌株)在巴氏消毒温度(65℃)及冷链温度(10℃)作用下的失活情况,并结合Weibull模型,拟合相应的失活参数(t_r值),探究了不同菌株间的失活异质性。在65℃处理条件下,19株副溶血性弧菌的t_r值介于22.62~67.23 s,VPC-1为耐热性最强菌株,而VPC-10为耐热性最弱菌株,热失活参数t_r值最适的概率分布为Normal (44.82, 12.27)。在10℃条件下,t_r值介于113.96~371.38 h,VPC-3为耐冷性最强菌株,VPC-2为耐冷性最弱菌株,冷失活参数t_r值最适的概率分布为Loglogistic (51.45,148.88,4.67)。结果表明,副溶血性弧菌的热失活和冷失活间没有显著的相关性,菌株的失活异质性广泛存在于副溶血性弧菌之中,仅基于单一菌株进行失活模型的拟合,很难描述其整体的失活趋势。同时,初步构建了菌株失活异质性的随机模型,并使用概率分布代替了传统的失活参数。

关键词：副溶血性弧菌巴氏消毒温度冷链温度失活异质性 Weibull模型

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A pathogenic fungus Alternaria mali was firstly identified from tea leaf spot disease in Kaiyang,Guizhou Province,China

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A pathogenic fungus Alternaria mali was firstly identified f...

A monograph on amylases from <i>Bacillus</i>spp.

中国植物病理学会2019年学术年会

作者： An Xiaoli Wang Xue Dharmasena Dissanayake-saman-pradeep ren Yafeng Wang Delu Song Baoan Chen Zhuo College of Forestry Guizhou University State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering Guizhou University

Leaf spot disease was an important disease of tea(Camellia sinensis) in Kaiyang county,Guizhou Province,which mainly damaged young leaves and shoot of tea and led to a huge loss of the production of *** spots initially represent brown and round,and then the diameter of the spot was 5-8 mm during later period,with the color of the center in the spot changing *** leaf spot disease always occurs in early spring and the region with 1300 m of *** 2016,disease incidence of leaves was estimated at 84% to 92%,depending on the *** order to identify the pathogens of this disease,we isolated and identified the pathogens from tea leaf spot disease in this *** gene of the internal transcribed spacer(ITS),parts of the 18 S nrDNA(SSU),translation elongation factor1-alpha(EF1),the Alternaria allergen a1(Alt),glyceraldehyde-3-phosphate dehydrogenase(GDP),28 S nrDNA(LSU),anonymous region(OPA1,OPA2) and endopolygalacturonase(endo)of the isolates were amplified,sequenced and deposited in *** to the Koch’s postulate,the pathogenicity test was conducted on tea leaves using the methods of the puncture,cut and *** result indicated that the isolates on PDA,OA and MEA medium represented initially round form,and white mycelium on PDA and MEA,and black brown mycelium on *** reverse sides of the isolates firstly displayed light yellow on PDA and MEA,and then rapidly became dark brown from the center on *** represent dark brown,geniculate,lenth:11.98±9.14 μm,width;4.47±0.64μm,ranging from 4 to 39×3 to 5 μm length/*** conidia,narrow ovoid,lenth;22.94±4.48 μm,width;11.09±1.68;14 to 34×7 to 14 μm length/width,with 4 to 8 transverse septa and with conspicuously ornamented *** parsimony phylogenetic analysis based on concatenated sequences of combined OPA1(1-610),OPA2(611-1200) and endo(1201-1658) indicated that the strain AXLKY01910 was identical to reference strains Alternaria mali strain EGS38-029,and the cl

关键词： Alternaria mali identification pathogenicity test

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Advances in Bioscience and Biotechnology 2013年第2期4卷 227-241页

作者： Sailas Benjamin r. B. Smitha V. N. Jisha S. pradeep S. Sajith S. Sreedevi Prakasan Priji K. N. Unni M. K. Sarath Josh Enzyme Technology Laboratory Biotechnology Division Department of Botany University of Calicut Kerala India

Owing to the production of alpha, beta and gamma amylase subtypes;starch degrading microbes, especially bacteria have an invincible role in the food, fermentation, textile and paper industries. Of them, α-amylases from Bacillus spp. have contributed tremendous advancements in bio-industry, especially in starch, detergent and pharmaceutical arena. Though general reviews are seen in literature on amylases, no focused review is available yet solely on α-amylases produced by Bacillus spp. Hence, this focused review on α-amylases from the genus Bacillus is designed in such a way that it should give a vivid picture on most of the aspects on bacillial α-amylases in a handy module with an industrial perspective. With a short introduction on amylases in general, α-amylases from various species of Bacillus reviewed herein encompasses production of α-amylases by submerged and solid-state fermentations;nutrients and other factors required for maximizing production;immobilization strategies for whole cells or purified enzyme;an overview on the molecular weight of the enzyme;followed by distinct sections for purification, characterisation, stability and crystal structure;and concluded with a section on industrial applications of the α-amylases from Bacillus spp.

关键词： Bacillus Amylases Production Fermentation Crystal Immobilization Stability Molecular Weight Characteristics Applications

Cognitive radio Sensing Using Hilbert Huang Transform

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Wireless Engineering and Technology 2010年第1期1卷 36-40页

作者： K. A. Narayanankutty Abhijith A. Nair Dilip Soori Deepak pradeep V. ravi Teja Vishnu K.B. Electronics and Communication Engineering Department Amrita School of Engineering

Vast segments of the frequency spectrum are reserved for primary (licensed) users. These legacy users often un-der-utilize their reserved spectrum thus causing bandwidth waste. The unlicensed (secondary) users can take advantage of this fact and exploit the spectral holes (vacant spectrum segments). Since spectrum occupancy is transient in nature it is imperative that the spectral holes are identified as fast as possible. To accomplish this, we propose a novel adaptive spectrum sensing procedure. This procedure scans a wideband spectrum using Hilbert Huang Transform and detects the spectral holes present in the spectrum.

关键词： Empirical Mode Decomposition (EMD) Ensemble Empirical Mode Decomposition (EEMD) Intrinsic Mode Function (IMF) Noise Assisted Data Analysis (NADA) Cognitive radio Guard Band Spectrum Sensing