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Agrobacterium Tumefaciens Based Transformation of Pelargonium x Hortrum cv. ‘Samba’ with Anti-1-aminocyclopropane-1-carboxylate Synthase cDNA to Regulate Ethylene Biosynthesis

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Engineering（科研） 2012年第10期4卷 4-7页

作者： Rajinder S. Ranu Jianguo Fan Sarada Krishnan pradeep agarwal. Amitva Mitra Colorado Sate University Laboratory of Plant Molecular Biology/Biotechnology Fort Collins CO USA 80523. Department of Plant Pathology The University of Nebraska Lincolin NE USA 68583-0722

Phytohormone, ethylene plays an important role in plant growth and development including fruit ripening and flower senescence. The synthesis of 1-aminocylo-propane-1- carboxylate (ACC), the immediate precursor of ethylene, from S-adenosyl-methionine is catalyzed by ACC synthase;and which is also a rate limiting step in the ethylene biosynthetic pathway. Therefore, it plays a key role in ethylene biosynthesis and the genes that code for ACC synthase are of special interest. Moreover, in zonal geraniums, ethylene bursts released from cuttings can have profound impact on the viability of explants for plant propagation. Biotechnological approach involving genetic modification that may reduce ethylene levels has potential for increasing the shelf-life of cuttings for plant propagation. These considerations have led us to clone several cDNA of ACC synthase genes from Pelargonium x hortorum cv. ?‘Sincerity’. To transform geranium cells with Agrobacterium tumefaciens an in vitro regeneration? system was developed using very young petiole explants. An Antisense construct of ACC synthase cDNA (PHSacc41) ligated into binary vector pAM696 was introduced into A. tumefaciens EHA 105 cells. Petiole explants were incubated with the Agrobacterium for 15 min and then co-cultivated for several days on MS medium containing 5 mM BAP and ?1 mM IAA in? the dark without the antibiotics. Selection for transformants was carried out in the presence of kanamycin and timentin. Transgenic plantlets generated were examined for inserted gene cassette by PCR and Southern blotting. Recovery of positive transformants that survived selection suggested that it is possible to transform and introduce genes via transformation in hybrid geraniums for genetic modification.

关键词： ACC Synthase Ethylene Biosynthesis Transformation Geranium Regeneration Agrobacterium Tumefaciens

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Free Vibration Analysis of RC Box-Girder Bridges Using FEM

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Sound & Vibration 2022年第2期56卷 105-125页

作者： Preeti agarwal.Priyaranjan Pal pradeep Kumar Mehta Motilal Nehru National Institute of Technology Allahabad Prayagraj211004India

The free vibration analysis of simply supported box-girder bridges is carried out using the finite element *** fundamental frequency is determined in straight,skew,curved and skew-curved box-girder *** is important to analyse the combined effect of skewness and curvature because skew-curved box-girder bridge behaviour cannot be predicted by simply adding the individual effects of skewness and *** first,an existing model is considered to validate the present approach.A convergence study is carried out to decide the mesh size in the finite element *** exhaustive parametric study is conducted to determine the fundamental frequency of box-girder bridges with varying skew angle,curve angle,span,span-depth ratio and cell *** skew angle is varied from 0°to 60°,curve angle is varied from 0°to 60°,span is changed from 25 to 50 m,span-depth ratio is varied from 10 to 16,and single cell&double cell are used in the present study.A total of 420 bridge models are used for parametric study in the *** shapes of the skew-curved bridge are also *** fundamental frequency of the skew-curved box-girder bridge is found to be more than the straight bridge,so,the skew-curved box-girder bridge is *** present study may be useful in the design of box-girder bridges.

关键词： Fundamental frequency skew-curved box-girder bridge single-cell double-cell curve angle skew angle FEM

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凝结热对低阶煤低温氧化过程的影响

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燃料化学学报 2006年第4期34卷 408-411页

作者：李永昕薛冰李再峰陈兴权 pradeep K agarwal 江苏工业学院化工系江苏常州213016 湛江师范学院科技开发中心广东湛江524048 美国怀俄明大学化工系美国怀俄明820713295

选用Pulse Calorimeter仪器，研究了低阶煤在干燥氧气下低温氧化过程的反应热和相对湿度为80％的氮气下凝结热与温度的变化，以研究凝结热对低阶煤低温氧化过程的影响。结果表明，随着温度的上升体系的反应热增加，而凝结热减少。在26℃... 详细信息

选用Pulse Calorimeter仪器，研究了低阶煤在干燥氧气下低温氧化过程的反应热和相对湿度为80％的氮气下凝结热与温度的变化，以研究凝结热对低阶煤低温氧化过程的影响。结果表明，随着温度的上升体系的反应热增加，而凝结热减少。在26℃～60℃的低温下，体系的凝结热明显高于反应热。因此，低温下凝结热是影响低阶煤的低温氧化过程的重要因素。研究还得到了低阶煤在干燥氧气下低温氧化过程的动力学方程及活化能。

关键词：低阶煤凝结热低温氧化

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Development and validation of microbial bioassay for quantification of Levofloxacin in pharmaceutical preparations

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Journal of Pharmaceutical Analysis 2015年第1期5卷 18-26页

作者： Nishant A.Dafale Uttam P.Semwal Piyush K.agarwal.pradeep Sharma G.N.Singh Microbiology Division Indian Pharmacopoeia Commission

The aim of this study was to develop and validate a simple,sensitive,precise and cost-effective onelevel agar diffusion（5＋1） bioassay for estimation of potency and bioactivity of Levofioxacin in pharmaceutical preparation which has not yet been reported in any *** 16 microbial *** pumilus ATCC-14884 was selected as the most significant strain against *** was optimized by investigating several factors such as buffer pH,inoculums concentration and reference standard *** of Levofioxacin in commercial sample Levoflox tablet was done by FTIR *** potency recovery value for Levofioxacin in Levoflox tablet was estimated as 100.90%.A validated bioassay method showed linearity（r^2 = 0.988）,precision（Interday RSD=1.05%,between analyst RSD=1.02%） and accuracy（101.23%,RSD=0.72%）.Bioassay was correlated with HPLC using same sample and estimated potencies were 100.90% and 99.37%.*** show that bioassay is a suitable method for estimation of potency and bioactivity of Levofioxacin pharmaceutical preparations.

关键词： Levofloxacin Antibiotic resistance Microbiological bioassay HPLC Pharmacopoeia

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Diagnostic Accuracy of IS6110 Insertion Gene, <i>Hsp65</i>, and Xpert MTB/RIF for Rapid Diagnosis of Pulmonary Tuberculosis

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Journal of Tuberculosis Research 2017年第1期5卷 1-12页

作者： Aymen Awad Abdelhaleem Almonther Abdallah Hershan pradeep Kumar agarwal Endemic and Tropical Diseases Unit Medical Research Center Jazan University Jazan Saudi Arabia Faculty of Medical Laboratory Sciences Alneelain University Khartoum Sudan Faculty of Medicine Jazan University Jazan Saudi Arabia Jazan Chest Disorders Hospital Jazan Saudi Arabia

Purpose: To evaluate diagnostic accuracy of IS6110 insertion genes, hsp65, and Xpert MTB/RIF for rapid diagnosis of pulmonary tuberculosis. Methods: Sixty patients, medically reported HIV negative, clinically suspected of having pulmonary tuberculosis, were included in this study, and consented before enrolment. Sputum samples were gathered once, and tested by smear for Acid Fast Bacilli (AFB). Cultured in the Loewenstein-Jensen (LJ) medium for M. tuberculosis growth, M. tuberculosis DNA was detected by conventional PCR targeting IS6110, and hsp65 genes using specific primers, and automated nested real-time PCR targeting rpoB gene. Sensitivity, specificity and diagnostic accuracy were calculated for each method compared to culture. Results: Compared with culture as reference method, smear, IS6110, hsp65, and Xpert MTB/RIF had sensitivity 77.14%, 100%, 100%, and 100%, specificity 92%, 96%, 96%, and 96.97%, and diagnostic accuracy 83.33%, 98.33%, 98.33% and 98.21% respectively. Molecular diagnostic methods had the highest diagnostic accuracy, whereas smear had the lowest. No statistical significance, (p value > 0.05) was detected between the patients’ demographic data and the presence or absence of TB infection. Conclusion: The diagnostic accuracy that we got from the molecular methods, confirmed the diagnostic value of molecular detection of M. tuberculosis in pulmonary cases, supporting the application of automated and conventional PCR in rapid analysis. Smear could be more efficient when used for treatment monitoring. Combination between one-molecular techniques with smear as a routine method could be valid for rapid diagnosis of TB.

关键词： Rapid Diagnosis Pulmonary TB Molecular Tests

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相对湿度对低阶煤低温氧化过程的影响

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煤炭转化 2007年第4期30卷 5-8页

作者：薛冰柳娜李永昕 pradeep K agarwal 江苏工业学院化工系江苏常州助教213164 美国怀俄明大学化工系美国怀俄明教授820713295

采用pulse calorimeter仪器,分别研究了在湿的氮气气氛下和湿的氧气气氛下的相对湿度对低阶煤低温氧化过程的影响.结果表明,在湿的氮气气氛下,相对湿度对凝结热(包括吸附热)有显著的影响,低阶煤低温氧化过程释放的热随着湿度的升高而增... 详细信息

采用pulse calorimeter仪器,分别研究了在湿的氮气气氛下和湿的氧气气氛下的相对湿度对低阶煤低温氧化过程的影响.结果表明,在湿的氮气气氛下,相对湿度对凝结热(包括吸附热)有显著的影响,低阶煤低温氧化过程释放的热随着湿度的升高而增加;同样在湿的氧气气氛下,随着湿度的增加体系放出的热明显增加;另一方面,随着温度的增加,体系的凝结热和吸附热减少;而在湿氧气下体系释放的热随温度的增加起始先减少后增加.

关键词：低阶煤低温氧化相对湿度

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低阶煤低温氧化过程热力学规律的研究

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煤炭转化 2007年第3期30卷 1-4页

作者：薛冰柳娜李永昕 pradeep K agarwal 江苏工业学院化工系江苏常州助教213016 美国怀俄明大学化工系美国怀俄明教授820713295

采用pulsecalorimeter仪器,分别研究了低温下低阶煤在湿的氮气气氛下凝结热与蒸发热间的热力学规律,以及在湿的氧气气氛下反应热与蒸发热间的热力学规律;还研究了低阶煤在干燥氧气下经三次低温氧化反应的热力学规律.结果表明,在湿的氮气... 详细信息

采用pulsecalorimeter仪器,分别研究了低温下低阶煤在湿的氮气气氛下凝结热与蒸发热间的热力学规律,以及在湿的氧气气氛下反应热与蒸发热间的热力学规律;还研究了低阶煤在干燥氧气下经三次低温氧化反应的热力学规律.结果表明,在湿的氮气下,凝结热和蒸发热均随温度的增加而减少,在温度299.15K^323.15K范围内,凝结热明显大于蒸发热值,随着温度增加二者差值变小;在湿氧气气氛下,随温度增加,反应热先减少后增加,其蒸发热与湿氮气条件相比,二者的热值及热力学规律基本相同;低阶煤经三次低温氧化反应过程,其中二、三次氧化反应的反应热与第一次相比显著降低.

关键词：低阶煤凝结热低温氧化

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低阶煤在湿的氧气下低温氧化过程热力学规律

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煤炭转化 2007年第2期30卷 1-5页

作者：李永昕薛冰柳娜 pradeep K agarwal 江苏工业学院化工系江苏常州博士教授213164 美国怀俄明大学化工系美国怀俄明教授820713295

采用pulse calori meter仪器,研究了低阶煤在湿的氧气下低温氧化过程的反应热,考察了在湿氧气下热释放速率与温度的关系,结果表明,其热力学规律与在干燥氧气以及湿氮气气氛下的热力学规律不同;在约299K(26℃)到333K,随着温度的增加,体... 详细信息

采用pulse calori meter仪器,研究了低阶煤在湿的氧气下低温氧化过程的反应热,考察了在湿氧气下热释放速率与温度的关系,结果表明,其热力学规律与在干燥氧气以及湿氮气气氛下的热力学规律不同;在约299K(26℃)到333K,随着温度的增加,体系放出的热减少;333K以后,随着温度的上升,体系的放热值增加.结果还表明,在低温(299K^333K)时,体系的放热以凝结热为主;而温度在333K以后,体系的放热以氧化反应热为主.

关键词：低阶煤低温氧化热力学

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Comment on “Factors affecting single-step transepithelial photorefractive keratectomy outcome in the treatment of mild, moderate, and high myopia:a cohort study”

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International Journal of Ophthalmology(English edition) 2023年第5期16卷 836-836页

作者： Suraj Kumar Chaurasiya Vishmarika Sharma pradeep agarwal Department of Optometry and Vision Science C L Gupta Eye InstituteRam Ganga Vihar Phase II(Extn.)Uttar PradeshMoradabad 244001India Department of Paediatric and Neuro Ophthalmology C L Gupta Eye InstituteRam Ganga Vihar Phase II(Extn.)Uttar PradeshMoradabad 244001India

Dear Editor,I would like to congratulate the author on their excellent paper. With great interest, I read Al-Mohaimeed et al’s study[1]“Factors affecting single-step transepithelial photorefractive keratectomy outco... 详细信息

关键词： mild treatment

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Comment on: Observation of peripheral refraction in myopic anisometropia in young adults

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International Journal of Ophthalmology(English edition) 2024年第7期17卷 1385-1386页

作者： Suraj Kumar Chaurasiya Mahendra Singh pradeep agarwal.Yu-Qin Du Yue-Hua Zhou Ming-Wei Ding Ming-Xu Zhang Yu-Juan Guo Shan-Shan Ge Department of Optometry and Vision Science CL Gupta Eye InstituteRam Ganga Vihar Phase II(Extn.)MoradabadUttar Pradesh 244001India Department of Paediatric and Neuro Ophthalmology CL Gupta Eye InstituteRam Ganga Vihar Phase II(Extn.)MoradabadUttar Pradesh 244001India Eye School of Chengdu University of TCM Ineye Hospital of Chengdu University of TCMChengdu 610036Sichuan ProvinceChina Beijing Ming Vision and Ophthalmology Beijing 100010China

Dear Editor,We read with great interest the recently published article titled“Observation of peripheral refraction in myopic anisometropia in young adults”by Du et al[1].The study conducted at the InEye Hospital of Chengdu University of TCM provides valuable insights into the relationship between anisometropia and peripheral refraction in myopic young *** commend the authors for their thorough investigation and adherence to ethical *** the study contributes significantly to our understanding of myopic anisometropia,we would like to draw attention to some limitations that merit consideration for a comprehensive interpretation of the findings.

关键词： Observation young insight

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