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Transposon-based genetic diversity assessment in wild and cultivated barley

The Crop Journal 2017年第4期5卷 296-304页

作者： Surinder singh prabhjot singh nandha Jaswinder singh Department of Plant Science McGill University 21111 Lakeshore Road Sainte-Anne-de-Bellevue Quebec H9X 3V9 Canada Department of Plant Sciences University of Saskatchewan 51 campus drive Saskatoon SK S7N 5A8 Canada

Transposable element-based molecular markers can be utilized to investigate genetic diversity and to create genetic linkage *** this study,Class I and class II transposons were employed to obtain a comparative account of genetic diversity between wild and cultivated barley *** types of PCR-based techniques were used:IMP(Inter MITE Polymorphism),IRAP(Inter-Retrotransposon Amplified Polymorphism)and REMAP(Retrotransposon-Microsatellite Amplified Polymorphism).Specific primer pairs for IMP,IRAP,and REMAP detected a total of 200 bands with an average of 20 bands per *** mean polymorphic information content(PIC)and discrimination power(D)values in all 47 genotypes from these three types of transposon-based polymorphisms were 0.910 and0.935,*** Pair Group Method with Arithmetic mean(UPGMA)-based cluster analysis classified all 47 genotypes,both wild and cultivated,into separate groups consistent with their geographical *** followed by chromosome location of polymorphic bands enables precise gene introgression from wild gene pool to cultivated *** highly polymorphic nature of these marker systems makes them suitable for use in varietal identification and MAS-based breeding programs in barley and other cereals.

关键词： IMP REMAP IRAP Polymorphic information content (PIC) Discrimination power (D)

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Peripheral primitive neuroectodermal tumor of the kidney presenting with pulmonary tumor embolism: A case report

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World Journal of Radiology 2014年第10期6卷 846-849页

作者： Sathya Chinnaa Chandan J Das Sanjay Sharma prabhjot singh Amlesh Seth Suvendu Purkait Sandeep R Mathur Department of Radiology All India Institute Of Medical Sciences Department of Urology All India Institute Of Medical Sciences Department of Pathology All India Institute Of Medical Sciences

Peripheral primitive neuroectodermal tumor(PNET) of the kidney is a rare, aggressive tumor known for its recurrence and metastatic potential. Despite the frequency of venous extension to the renal veins and inferior vena cava, pulmonary tumor embolism at the initial presentation is not common. We report a case of 22-year-old female with PNET of the kidney who presented with tumor embolism in the inferior vena cava(IVC) and bilateral pulmonary artery. The patient underwent surgical resection and histopathological analysis confirmed the presence of tumor within the IVC and pulmonary arteries. The patient received adjuvant chemotherapy and is currently doing well on follow-up.

关键词： Primitive neuroectodermal tumor Pulmonary tumor embolism Computed tomography

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